OBJECTIVE:To detect the contents of chlorogenic acid and rutoside in Saussurea involucrate,and to optimize the decoction and extraction technology of S. involucrate from different producing areas. METHODS:The contents of chlorogenic acid and rutoside in 10 batches of S. involucrate from different producing areas were determined by HPLC. L9(34)orthogonal experiment was used to optimize the water amount,decoction times and decoction time using comprehensive score of extraction transport rate of chlorogenic acid and rutoside as index. The verification test was also conducted. RESULTS:The contents of chlorogenic acid and rutoside in 10 batches of S. involucrate were 0.380%-0.546% and 0.334%-0.617%;the optimal decoction technology was as follows as the amout of crude material of S. involucrate 100 g,soaking for 20 min,decocting for 3 times,12,10 and 10 fold of water,decocting 45,30 and 30 min,respectively. The extraction transfer rates of chlorogenic acid and rutoside were 96.2%(RSD=2.66%,n=3)and 89.3%(RSD=3.31%,n=3)in verification test. CONCLUSIONS:For S. involucrate from different producing areas,the contents of effective components are different;optimized decoction and extraction technology is stable and feasible.

Objective To analyze the therapeutic effect of three-dimensional conformal involvedfield radiotherapy (3D-CRT)combined with chemotherapy on limited disease stage small cell lung cancer (LD-SCLC).Methods The clinical data of 85 patients of LD-SCLC treated with 3D-CRT at the dose of 2 Gy/fraction,5 fractions per week for 5 - 7 weeks,with the median dose of 50 Gy ( 46 - 66 Gy),combined with 4 -8 cycles chemotherapy,64 males and 21 females,aged 29 -76,were collected and analyzed.Results The complete remission rate,partial remission rate,stability rate,and total effective rate were 36.5％,52.9％,10.6％,and 89.4％,respectively.The median survival time was 18 months,with the 1-,2-,and 3-year overall survival rates of 65.9％,33.8％,and 15.9％,respectively.The local recurrence rate,distant metastasis rate,and local recurrence + distant metastasis rate were 15.2％ (9/85),49.2％ (29/85),and 35.6％ (21/85),respectively.Body weight,response to therapy,cycles for chemotherapy,and concurrent chemo-radiotherapy were all independent prognostic factors for LD-SCLC.Cox muhivariable regression was used to analyze the prognostic factors.Conclusions Involved-field radiotherapy is effective for LD-SCLC.Distance metastasis is the main cause of treatment failure.

Objective The relationship between toll like receptor 4 (TLR4) the single nucleotide polymor-phisms of gene (SNP) and ischemic stroke reperfusion injury was evaluated by meta-analysis ,which was de-signed to provide evidence-based medicine for the prevention of ischemic stroke reperfusion injury . Methods In Medline ,PubMed ,EMBASE ,Cochrane ,CBM ,Chinese Journal Net ,academic conference materials and dissertations ,we searched for comprehensive information on the relationship between TLR4 gene SNP (rs10759932 ,rs11536891 ,rs11536879) in ischemic stroke Cohort study and case-control literature to determine whether the gene SNP (rs10759932 ,rs11536891 ,rs11536879) was associated with ischemic stroke reperfusion injury by genotype comparison .The heterogeneity test was performed by Stata11 .0 .The heterogeneity test was used to calculate the OR value .The heterogeneity between different studies was analyzed quantitatively . The fixed effect model was used and the percentage I2 was calculated .Results Meta analysis showed that 1943 cases of ischemia-reperfusion injury and 5043 cases of control group were analyzed ,TLR4 gene SNP (rs10759932 ,rs11536891 ,rs11536879) was associated with the risk of ischemic stroke reperfusion injury ,the dominant fixed effect models were (OR=1 .653 ,95％ CI:1 .416 -1 .930 ;OR=1 .653 ,95％ CI:1 .416 -1 .930 ;OR=1 .653 ,95％ CI:1 .416-1 .930 ;);a co-dominant fixed effect model (OR=1 .525 ,95％ CI:1 .350 -1 .723 ;OR= 1 .653 ,95％ CI:1 .416 -1 .930 ;OR= 1 .653 ,95％ CI:1 .416 -1 .930) .Conclusion TLR4 gene SNP (rs10759932 ,rs11536891 ,rs11536879) was associated with the occurrence of ischemic stroke reperfusion inju-ry by Meta-analysis .