Objective To investigate the influencing factors for late-preterm birth of twins. Methods We retrospectively analyzed the clinical data of 301 twins delivered in Beijing Haidian Maternity and Child Health Hospital between August 1, 2011 and August 31, 2013. Twins with late-preterm births were classified as the observation group(n=138) and those delivered at term were classified as the control group (n=163). Comparison between the two groups was conducted on both maternal and neonatal complications. Two independent sample Student's t test, Chi-square test and logistic regression analysis were performed for statistical analysis. Results (1) The ratio of two male fetuses, dichorionic diamniotic twins, and iatrogenic preterm birth were 41.3% (57/138), 76.1% (105/138) and 65.2% (90/138), respectively, in the observation group, while 23.9% (39/163), 89.6% (146/163) and 96.9% (158/163), respectively, in the control group (χ2=10.40, 9.81 and 53.59, respectively, all P<0.05). Mean birth weight of twins in the observation group was less than that in the control group [(2 450±349) vs (2 640±304) g, t=7.12, P<0.05]. Maternal age, gravidity, parity, history of abortion, mode of conception and 1 min Apgar score of the neonates between the two groups were not significantly different (all P>0.05). (2) The risk factors for late-preterm birth of twins included hypertension diseases complicated pregnancy, gestational diabetes mellitus, premature rupture of membranes, fetal distress, anemia and umbilical cord abnormality [OR=0.190(95%CI: 0.094-0.387), 1.980 (95%CI: 1.009-3.883), 0.030(95%CI: 0.007-0.131), 0.062(95%CI: 0.006-0.642), 0.470(95%CI:0.243-0.908) and 2.779(95%CI:1.093-20.736), respectively, all P<0.01 or 0.05]. (3) The incidences of hyperbilirubinemia, respiratory diseases, hypoglycemia, anemia, and neonatal infection were 27.9%(77/276), 4.4%(12/276), 4.7%(13/276), 4.4%(12/276) and 3.6%(10/276), respectively, in the observation group, which were all higher than those in the control group [9.5%(31/326), 0.6%(2/326), 1.2%(4/326), 0.6%(2/326) and 0.3%(1/326), respectively,χ2=34.33, 9.18, 6.61, 9.18 and 9.16, respectively, all P<0.05]. The incidence of small for gestational age in the observation group was lower than that in the control group [3.6%(10/276) vs 12.3%(40/326),χ2=3.86, P<0.05]. Other complications in the two groups (intracranial hemorrhage, acidosis, and hemolysis) were not significantly different (all P>0.05). Conclusions Late-premature births in twin pregnancies are related to many maternal and fetal risk factors. Therefore, clear understanding of these risk factors might improve the pregnancy outcomes.

We described a simple method for determining glycated lipoproteins (glc Lps) in serum by agarose gel film electrophoresis, and color development with nitroblue tetrazolium. The resulting blue bands on the film were measured densitometrically at 545 nm to quantify ?-, pre-?-, and ?-glc Lps. The concentration of gfc ?-Lp in serum from diabetics was 2,2-fold higher than that in normal individuals. Diabetic patients with vascular complications had higher concentrations of glc ?-Lp than did patients without vascular complication. The concentration of glc ?-Lp (glc LDL) in serum may be a useful diagnostic indicator of diabetic vascular complications and their severity.

Aortic collagen advanced glycosylation endproduct (AGE) and collagen contents were determined in 8 streptozotocin-induced diabetic rats and 8 control rats. Ten weeks after a peritoneal injection of streplozotocin, the AGE and collagen contents were significantly increased in diabetic rats (P

Objective To study the microanatomy of the petrosal vein(PV) and discuss the surgical management of the vessel during microvascular decompression.Methods A total of 15 cadaveric(30 PVs) heads were obtained from dead adults.The position,morphology,branches,and variation of the PV,and anatomical relationship between the trigeminal,facial and acoustic nerves and the vessel were observed.Sixty patients with trigeminal neuralgia or hemifacial spasm were also enrolled in this study.Microvascular decompression was performed on the cases,and the PV and its branches were observed during the operation.Results The PV is formed by the union of 2 to 3 branches.It passes through the the subarachnoid space and terminates in the inner two third of the superior petrosal sinus.Classfied by number of the unilateral PVs,3 types of the vessel has been observed:single-stem(9 sides,30.0%),double-stem(17 sides,56.7%) and triple-stem types(4 sides,13.3%).Moreover,according to the anatomical relationship between the end of the PV and auditory meatus,the vein has 3 subgroups:interior-side(17/55,30.9%),intermediate(24/55,43.6%),and exterior-side(14/55,25.5%) subgroups.In our specimens,8 PVs(the main stem or a branch) touched the trigeminal nerve,and 19 PVs were less than 1 mm away from the nerve.None of the PVs connected to the faical and acoustic nerves.In the 60 patients who recieved microvascular decompression,107 PV branches(19 single-stem veins,35 double-stem vessels,and 6 triple-stem veins) were found during the operation,among which 33 belonged to interior-side subgroup,46 were classifed as intermediate type,and 28 were exterior-side veins.Conclusions Management of the PV is the key step for cerebellopontine angle surgery.The vein or its branches can be cut off when they compress the surrounding tissues or obstruct the surgical field.

With the rapid development of genome sequencing technologies, a large amount of prokaryote genomes have been sequenced in recent years. To further investigate the models and functions of genomes, the algorithms for genome annotations based on the sequence and homology features have been widely implemented to newly sequenced genomes. However, gene annotations only using the genomic information are prone to errors, such as the incorrect N-terminals and pseudogenes. It is even harder to provide reasonable annotating results in the case of the poor genome sequencing results. The transcriptomics based on the technologies such as microarray and RNA-seq and the proteomics based on the MS/MS have been used widely to identify the gene products with high throughput and high sensitivity, providing the powerful tools for the verification and correction of annotated genome. Compared with transcriptomics, proteomics can generate the protein list for the expressed genes in the samples or cells without any confusion of the non-coding RNA, leading the proteogenomics an important basis for the genome annotations in prokaryotes. In this paper, we first described the traditional genome annotation algorithms and pointed out the shortcomings. Then we summarized the advantages of proteomics in the genome annotations and reviewed the progress of proteogenomics in prokaryotes. Finally we discussed the challenges and strategies in the data analyses and potential solutions for the developments of proteogenomics.
Genomics ; Molecular Sequence Annotation ; Prokaryotic Cells ; metabolism ; Proteomics ; Tandem Mass Spectrometry

Objective:To evaluate the value of 2D-3D registration method which combines improved mutual information and image pyramid.Methods:The continuous image representation of the cubic B-spline curve and Parzen histogram estimation were fused into the algorithm. The chest was used as the research object. The reconstruction of the orthogonal X-ray image generated by the radiographic image and the image after a certain transformation with itself were used for registration experiments to study the registration accuracy and time.Results:After 50 sets of controlled experiments, compared with the traditional registration method, the displacement accuracy of this method in the X and Y directions was improved by 53.39% and 21.33%, and the registration time was shortened by 91.93%. Compared with the modified algorithms in recent years, the displacement accuracy of the improved algorithm in the X and Y directions was increased by 17.65% and 13.79%. And the registration time was further increased by 19.64%.Conclusions:This method can effectively improve the registration accuracy and efficiency of 2D and 3D images, and both meet the requirements of image registration within 2 mm during surgery. The high efficiency and accuracy of this method provide beneficial information for clinical diagnosis and radiotherapy automation, which also lays the foundation for tumor position error correction and automatic positioning of medical robotic arms.

Objective To investigate the effect of recurrent patella dislocation by grafting dual-bundle semitendinosus to anatomically reconstruct of medial patellofemoral ligament(MPFL)under arthroscopy.Methods From January 2006 to January 2010,29 cases(31 knees)with recurrent dislocation of the patella were performed arthroscopic surgery,including 11 males and 18 females(20 knees)with an average age of 22 years(range,16-32).The key point of surgery was reconstruction MPFL with dual-bundle free autogenous semitendinosus by using two anchors and bone groove to fixed on the patella,and using the interference screw to fixed on the femur.Adjusting the patella reset under arthroscopy.Tibial tubercle were transferred to inside in 5 cases.All patients were followed up for an average of 14 months(ranging 9-22).The imaging evaluation included congruence angle,lateral patellofemoral angle and lateral shift.The clinical therapeutic effect was evaluated with International Knee Documentation Committee(IKDC),Lysholm and Tegner scores.Results The fear test was negative after operation.There was no redislocation and fracture of the patella during follow-up.The congruence angle,lateral patellofemoral angle and lateral shift were 5.65°±2.23°,3.52°±2.63° and 0.25±0.46 respectively at the final follow-up.The mean IKDC,Lysholm and Tegner scores were 93.20±5.33,93.02±6.08 and 6.58±0.87 respectively at the final follow-up.All were showed statistically significant differences compared with preoperation(P＜0.05).Conclusion It is demonstrated that there was a reliable short-term effect to treat recurrent patella dislocation by grafting dual-bundle semitendinosus to anatomically reconstruct of MPFL under arthroscopy.

Objective To analyze the survey results of iodine nutritional level among women of childbearing age,pregnant,lactating women and infants before and after the adjustment of iodine concentration in salt of Zibo City,in order to provide a scientific basis for reasonable iodine supplement.Methods The iodine content in the residents' edible salt samples was determined in 2011 and 2015.Four groups of target populations including women of childbearing age,pregnant,lactating women and infants from 7 districts and towns of Zibo were investigated to test their urinary iodine level in 2011 and 2015.The monitoring results of the two years were compared and analyzed.Results A total of 2 100 and 2 160 residents edible salt were monitored in 2011 and 2015,and the median of salt iodine were 30.2 and 22.7 mg/kg,there was statistically significant difference in the constitute of salt iodine level between the two years (x2 =141.90,P ＜ 0.01).A total of 354,402,403,362 urine samples of women of childbearing age,pregnant,lactating women and infants' were detected and the median urinary iodine were 161.80,127.20,138.80,147.10 μg/L in 2011,and 350,706,350 and 352 of the corresponding target population's urine samples were detected and the median urinary iodine were 127.80,129.95,70.20 and 152.35 μg/L in 2015.Conclusions The salt iodine median has reduced and the urinary iodine in childbearing age women and infant is in the appropriate level,while it is insufficient in the pregnant women and lactating women after the adjustment of iodized salt concentration in Zibo.It is necessary to promote health education and instruct pregnant women and lactating women to supplement iodine,additionally,surveillance of the iodine nutrition status of the targeted populations should be strengthened.

BACKGROUND:Whether continuous passive motion improves osteoarthritis by enhancing the proliferation ability of chondrocytes is rarely reported.
OBJECTIVE:To analyze the therapeutic outcomes of continuous passive motion in rabbits with osteoarthritis and the underlying mechanism.
METHODS:Thirty-six New Zealand white rabbits were randomly al otted into three groups (n=12 per group). Rabbits in control group only underwent capsulotomy with no harm to the cartilage;osteoarthritis models were established in the rabbits of model and treatment groups using Hulth method. At 1 day after modeling, the treatment group rabbits were treated with continuous passive motion, 8 hours daily for consecutive 8 weeks. Interleukin-1 and tumor necrosis factorαlevels in the synovial fluid were detected by ELISA;col agen type II expression and the proliferation ability of chondrocytes were detected by MTT assay;Erk signaling pathway activation was determined using western blot assay.
RESULTS AND CONCLUSION:In the model group, interleukin-1 and tumor necrosis factorαlevels in the synovial fluid were significantly increased, and the expression level of col agen type II mRNA was remarkablely down-regulated. Continuous passive motion significantly downregulated interleukin-1 and tumor necrosis factorαlevels and up-regulated the col agen type II mRNA level (P<0.01). The model group showed significantly decreased proliferation ability of chondrocytes and down-regulated Erk signaling pathway activation, while after continuous passive motion, al above indicators were significantly improved (P<0.01). These results indicate that the continuous passive motion can al eviate osteoarthritis probably by influencing interleukin-1 and tumor necrosis factorαlevels, proliferation ability of chondrocytes, and col agen type II expression, as wel as regulating Erk signaling pathway activation.

Objective:To explore the lactate metabolism in brain tissue of the mice with early acute hypoxia-ischemia injury,and to provide data support for 9.4T 1 H-NMR spectroscopy in detecting the lactate level clinically.Methods:Eighty Kunming mice were randomly divided into sixteen groups (0 s,20 s,40 s,60 s,2 min,4 min, 6 min,8 min, 10 min, 12 min, 14 min, 16 min, 18 min,and 20 min)according to the duration of hypoxia-ischemia (n=5).The changes of lactate levels were detected by 9.4T 1 H-NMR spectroscopy. Results:After the initiation of hypoxia-ischemia injury,the lactate level began to increase rapidly to the highest value of (6.89 ± 0.34)μmol·g-1 at 20 s,then started to decline quickly from 40 s to 2 min,and eventually decreased to a stable level of (4.85±0.36)μmol·g-1 until 6 min.Compared with control group,the levels of lactate in brain tissue of the mice in hypoxic-ischemic groups were increased (P <0.01).Conclusion:40 s of acute hypoxia-ischemia may be the lactate cerebral neuron threshold during the anaerobic glycolysis. 9.4T1 H-MRS can provide the exact time window for detecting the lactate metabolism.