1.The Application of Compound Tabletop in Medical Imaging Equipment.
Yunping WANG ; Wei WU ; Wenting RUI
Chinese Journal of Medical Instrumentation 2015;39(3):190-191
This paper mainly introduces the material composition and structure of two kinds of compound tabletop which currently used in large medical imaging equipment, also introduces the proper material choosing and production processing, together with the advantage and shortage of this two kinds of tabletop and different application occasion.
Construction Materials
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Diagnostic Imaging
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instrumentation
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Durable Medical Equipment
2.THE EXPLORATION OF STANDARDIZED PROCESSES AND METHODS OF HOSPITAL COST ACCOUNTING
Lixin XU ; Yunping LIANG ; Minji WU
Modern Hospital 2015;15(5):133-135
Based on the study of cost accounting of several large public hospitals in Guangdong Province according to the New Hospital Financial Regulations, the paper presents a detailed operational and standardized cost accounting rules, including the specific processes and methods of cost accounting.It has laid a solid foundation for cost accounting, financial management and medical service pricing in hospitals.
3.Methods and Strategies of Novel Proteins Identification in Proteomics
Jie MA ; Songfeng WU ; Yunping ZHU
Progress in Biochemistry and Biophysics 2006;0(08):-
The combination of tandem spectrometry and database searching is one of the most popular technologies for protein identification.However,only those proteins in the searching database could be identified,and current database is far from completeness.So it is necessary to mining the MS/MS data comprehensively,in which novel protein identification is the most important one.The definition of novel protein could be divided into three levels according to their annotations of sequences and functions.As a part of protein identification,the main approaches used to identify novel protein are basing on the following two different ways:de novo sequencing combined with similarity search and searching against nucleotide acid databases such as EST or genome databases.Several mature or newly developed methods and techniques were summarized,and the problems and strategies discussed here would be helpful for the related researches.
4.The Practice of Teaching Project and Teaching Pattern in Medical Electronics
Libo CHEN ; Jiaming TONG ; Yunping WU
Chinese Journal of Medical Education Research 2003;0(03):-
This article is concerned about the practice and exploration on medical electronics teaching project and teaching pattern we have done before and the Active Participation Pattern thus formed. That is guiding, putting for ward questions, discussing, debating and summarizing in class while setting research problem after class. We also have made media courseware in medical electronics and have used it in our teaching process, which has aroused the students' interest in study and has enhanced the teaching efficiency and quality. This new method of teaching pattern helps us gain the strong feedback and affirmation and is welcome by the students.
5.Defense mechanisms and childhood abuse of patients with borderline personality disorder
Jianjun HUANG ; Jiang WU ; Yingjun XI ; Kai LI ; Yunping YANG
Chinese Journal of Health Management 2012;(6):363-366
Objective To understnad the defense mechanisms of patients with borderline personality disorder (BPD) and to explore its relationship with childhood abuses.Methods One hundred and forty-two outpatients with BPD (group A),63 outpatients with other personality disorders (group B) and 20 outpatients without any Axis Ⅱ diagnoses (group C) were enrolled and completed Chinese versions of the Childhood Experience of Care and Abuse Questionnaire (CECA.Q) and the Defensive Style of Questionnaire (DSQ).Multivariate analysis of variance and Pearson correlation analysis were used for data analysis.Results Compared to group B and C,the score of defense mechanisms reported by group A were significantly increased in all eight premature defense mechanisms,such as projection(F =26.5,P < 0.05),passive aggression (F =18.1,P < 0.05),acting out (F =28.3,P < 0.05),complaint (F =16.7,P <0.05),fantasy(F =16.3,P < 0.05),splitting (F =28.8,P < 0.05),withdrawal (F =8.3,P < 0.05),somatization(F=7.6,P < 0.05),and part of the middle defense mechanisms,such as omnipotence-devaluation(F =9.2,P < 0.05),isolation(F =4.7,P < 0.05) and identification (F =10.0,P < 0.05).At the same time,the BPD patients got lower scores in the mature defense mechanisms,such as sublimation (F =4.1,P < 0.05) and repression(F =3.9,P < 0.05).Those who experienced physical abuse use more complaint (F =6.9,P < 0.05),denial mechanisms (F =5.2,P < 0.05) and less anticipation one (F =4.6,P < 0.05).Those who experienced sexual abuse use more somatization (F =4.4,P < 0.05) and isolation mechanism (F =3.6,P < 0.05).Conclusions BPD patients would like to use more immature and middle defense mechanisms and less mature ones.Emotional,physical and sexual abuse from parents and other adult care takers have a correlation with immature defense mechanisms,which indicates the importance of care about parental rearing patterns and mental health of children.
6.Application of 9.4 T 1 H-MR spectroscopy in determination of lactate in brain of mice with early acute hypoxia-ischeia injury and its significance
Yunping ZHANG ; Yangkang LI ; Chenyang LIU ; Jiahui ZHU ; Renhua WU
Journal of Jilin University(Medicine Edition) 2016;42(4):690-693
Objective:To explore the lactate metabolism in brain tissue of the mice with early acute hypoxia-ischemia injury,and to provide data support for 9.4T 1 H-NMR spectroscopy in detecting the lactate level clinically.Methods:Eighty Kunming mice were randomly divided into sixteen groups (0 s,20 s,40 s,60 s,2 min,4 min, 6 min,8 min, 10 min, 12 min, 14 min, 16 min, 18 min,and 20 min)according to the duration of hypoxia-ischemia (n=5).The changes of lactate levels were detected by 9.4T 1 H-NMR spectroscopy. Results:After the initiation of hypoxia-ischemia injury,the lactate level began to increase rapidly to the highest value of (6.89 ± 0.34)μmol·g-1 at 20 s,then started to decline quickly from 40 s to 2 min,and eventually decreased to a stable level of (4.85±0.36)μmol·g-1 until 6 min.Compared with control group,the levels of lactate in brain tissue of the mice in hypoxic-ischemic groups were increased (P <0.01).Conclusion:40 s of acute hypoxia-ischemia may be the lactate cerebral neuron threshold during the anaerobic glycolysis. 9.4T1 H-MRS can provide the exact time window for detecting the lactate metabolism.
7.Ultrasonography quantitative indicator for position of fetal conus medullaris
Shaozheng HE ; Guorong LYV ; Junxian RUAN ; Jiaxiang WU ; Yunping LIU
Chinese Journal of Perinatal Medicine 2015;18(10):770-773
Objective To determine the normal position of the fetal conus medullaris and assess its clinical significance.Methods A total of 550 singleton pregnant women who were examined by fetal ultrasonography in the Department of Ultrasound at the Second Hospital of Fujian Medical University from June 1, 2013 to September 31, 2014 were included.Inclusion criteria were pregnancies with regular menstruation cycle and known last menstrual period, gestational age (GA) confirmed by ultrasonographic examinations, and neonate abnormality excluded by pediatrician after born.Routine obstetric ultrasound examinations were performed to measure the biparietal diameter (BPD), femur length (FL), head circumference (HC) and abdominal circumference (AC).The conus distance (CD) was determined by measuring the distance between the distal end of the conus medullaris and the caudal end of the last vertebra.The average value was calculated for each group according to GA.Fifty normal fetuses were randomly selected for quality control.For inter-observer reliability assessment, the same data were collected and analyzed by two different operators.For intra-observer reliability assessment, the data were collected and analyzed twice by the same operator with an interval of half an hour.Linear regression correlation analysis was performed to analyze the relationship between CD and GA, BPD, FL, HC and AC.Intraclass correlation coefficient (ICC) and Bland-Altman analysis were used to analyze the repeatability of the analysis.Results In 518 (94.2%) of 550 fetuses, the CD was successfully measured.Positively correlations between the conus distance and the gestational age was observed.The CD was (10.0± 3.3) mm at > 14-≤ 15 weeks (n=17), (27.7±3.8) mm at > 20-≤ 21 weeks of gestation (n=18), (41.5±2.4) mm at > 26-≤ 27 weeks (n=8), (54.7±3.0) mm at > 32-≤ 33 weeks (n=17), and (71.9±2.7) mm at > 41-≤ 42 weeks (n=6).Linear regression correlations between CD (mm) and FL, AC, HC, BPD and GA were:CD=1.04× FL (mm)-8.71, CD=0.23 × AC (mm)-10.11, CD=0.28× HC (mm)-18.10, CD=0.90× BPD (mm)-17.65, CD=2.34× GA (weeks)-20.94 (r=0.990, 0.985, 0.978, 0.974 and 0.973, respectively, all P < 0.01).The measurement of the conus distance exhibited good reproducibility between different operators (ICC=0.984, 95%CI: 0.972-0.991) and for the same operator (ICC=0.992, 95%CI: 0.985-0.995) with the 95% limits of agreement of-1.8 to 3.5 mm and-4.3 to 2.1 mm.Bland-Altman analysis showed that most of the measured values were within 95% confidence interval suggesting good consistency.Conclusions The distance, between the end of the conus medullaris and the end of the last vertebral body under ultrasound scan, is positively correlated with FL and other ultrasonographic indicators, which implies that it could be used as a new index for position of fetal conus medullaris.
8.Analysis of clinical characteristics between patients with idiopathic pulmonary fibrosis combined with emphysema and without emphysema
Yunping WU ; Wei LIU ; Zhihe ZHOU ; Dong WANG ; Wenjuan MA
Chinese Journal of Postgraduates of Medicine 2014;37(28):27-30
Objective To investigate the differences of clinical features,pulmonary function tests,chest imaging and prognosis between patients with idiopathic pulmonary fibrosis (IPF) combined with emphysema and without emphysema.Methods A total of 43 patients with IPF and with pulmonary fibrosis and emphysema (CPFE) were selected as observation group,and 40 patients with IPF and without emphysema were selected as control group.The clinical data of two groups of patients,including pulmonary function,partial pressure of oxygen,cellular components of bronchoalveolar lavage fluid (BALF),survival time were retrospectively analyzed and compared between two groups.Results The smoking index was (29 ± 17) pack years in observation group,much higher than that in control group (17 ± 15) pack years,the difference was statistically significant (P < 0.05).The percentage of forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) was (70 ± 6)% in observation group,significantly lower than that in control group (84 ± 9)%,the difference was statistically significant (P < 0.05).The percentage of diffusion capacity of carbon monoxide (DLCO) in observation group was (43 ± 11)%,significantly lower than that in control group (55 ± 17)%,the difference was statistically significant (P < 0.05).The percentage of lung volume in observation group was (78 ± 12)%,significantly higher than that in control group (63 ± 11)%,the difference was statistically significant (P <0.05).The vital capacity was expected to account for partial pressure had no significant difference in percentage and resting arterial oxygen value between two groups (P > 0.05).The total number of BALF cells,macrophages,neutrophils,lymphoeytes and eosinophils ratio had no significant difference between two groups (P > 0.05).Conclusion Smoking is an important risk factor of IPF with emphysema,emphysema does not affect the prognosis of patients with IPF.
9.Screening of differently expressed genes in human prostate cancer cell lines with different metastasis potentials.
Anping, SONG ; Guoning, LIAO ; Mingfu, WU ; Yunping, LU ; Ding, MA
Journal of Huazhong University of Science and Technology (Medical Sciences) 2007;27(5):582-5
In order to screen the genes differentially expressed in two human prostate cancer cells with different metastasis potentials, suppression subtractive hybridization (SSH) was done twice on human prostate cancer cell line with high potential of metastasis PC3M-1E8 and its synogenetic cell line PC3M-2B4 with low metastasis potential. In the first subtraction PC3M-2B4 was used as tester and PC3M-1E8 as driver and the forward subtractive library was constructed. In the second on the tester and driver were interchanged and the reverse subtractive library was constructed. The screened clones of both libraries were sequenced and Gene Bank homology search was performed. Some clones were confirmed by quantitative real-time PCR. The results showed that two subtractive libraries containing 238 positive clones were constructed. Analysis of 16 sequenced clones randomly picked from two libraries showed that 4 differentially expressed gene fragments were identified as new EST with unknown functions. It was concluded that two subtractive libraries of human prostate cancer cell lines with different metastasis potentials were constructed successfully.
Cell Line, Tumor
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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Gene Library
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Neoplasm Metastasis/*genetics
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Nucleic Acid Hybridization/*methods
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Prostatic Neoplasms/*genetics
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Prostatic Neoplasms/pathology
10.Metabonomics study of urine samples of patients with hyperlipidemic pancreatitis
Yan ZHAO ; Jianbing WU ; Li PAN ; Xiaolei ZHANG ; Yunping QIU ; Mingming SU ; Wei JIA ; Xingpeng WANG
Chinese Journal of Pancreatology 2009;9(2):85-88
Objective Metabonomics method based gas chromatography-mass spectrometry (GC/MS)were used to analyze the urine samples of patients with hyperlipidemic pancreatitis (HLP) to describe the characteristics of metabolism changes of HLP,identify potential biomarkers,and investigate the role of metabonomics study in the management of AP.Methods 24 patients of HLP and 40 age,sex matched volunteers were enrolled and their urine samples were collected.The urine samples underwent preparation,derivation and GC/MS analysis,Orthogonal-Projection to Latent Structures-Discriminant Analysis (OPLS-DA)were performed to detect the metabolic profile difference between the HLP and control group.Results HLP patients can be precisely distinguished from healthy controls.21 metabolites (credibility > 700 ) were identified using the reference compounds available in the libraries of NIST and Wiley.It was identified that levels of nicotinic acid,aconitie acid,citric acid,hippurie acid,hydroxyphenylacetic acid,hydroxyphenylpropionicacid were decreased,while the levels of tryptophan,tyrosine,tyramine,16-hexadecanoic acid,18octadecanoie acid were increased.It was also suggested that there was change in tricarboxylic acid cycle and gut bacterial flora,as well as fat metabolism and metabolism of amino acid.Conclusions There are differences between healthy controls and HLP patients in the term of GC/MS metabolic profiling,and the biomarkers in the metabolites could be found through metabonomics analysis,and the mechanisms of the metabolic changes could be explored.It was noted that the research of metabolites in the urine samples may be a useful tool to help diagnose and understand the pathogenesis of HLP.Metabonomics analysis is a promising research method.