1.The Relationship Between the Dynamic Change of High-sensitivity C-reactive Protein,IL-1?,IL-6 and the Prognosis of the Patients with Acute Traumatic Brain Injury
Conglin JIA ; Zhiping SI ; Yunlong TONG
Journal of Medical Research 2006;0(11):-
Objective To study the dynamic change of high-sensitivity C-reactive protein(hCRP),interleukin-1(IL-1?)interleukin-6(IL-6)in acute traumatic brain injury,and the correlation between the dynamic change and the scale of brain injury and the prognosis of the patients.Methods 192 cases patients were divided into different groups according to the Glasgow Coma Scale(GCS)and the Glasgow outcome Scale(GOS)three months later discharged from hospital respectively.The concentration of serum CRP,IL-1?,IL-6 were detected with immunoturbidimetry and ABC-ELISA 24 with in hours after traumatic brain injury and the concentration of serum after iniwn at ond day,three days and seven days was detected as well.Results Serum hCRP levels were remarkably elevated and reached peak value within 24 hours,and then gradually decreased.There were a negative correlation between hCRP,IL-1?,IL-6 and the patient's GCS groups(r=-0.162,P
2.Detection of Single Nucleotide Polymorphism by Real-time Polymerase Chain Reaction Coupled with High Specific Invader Assay in Single Tube
Menglin ZHENG ; Xiemin QI ; Huan TONG ; Yunlong LIU ; Bingjie ZOU ; Qinxin SONG ; Guohua ZHOU
Chinese Journal of Analytical Chemistry 2015;(7):1001-1008
A method for the real-time polymerase chain reaction ( PCR ) coupled with high specific invader assay to detect single nucleotide polymorphism ( SNP) was established. To reduce the background signal, the amount of flap endonuclease 1 ( FEN1 enzyme ) and wild-type detection probe was optimized. Under the optimum conditions including 0. 05 μmo/L invasive oligonucleotide probe, 0. 125 μmol/L wild-type detection probe, 0. 5 μmol/L mutation detection probe, 0. 25 μmol/L each fluorescence resonance energy transfer (FRET) probe and 1. 5 U FEN1, the background signal of wild-type sample and mutation sample was dramatically decreased and the background interference to the detecting results was thus eliminated. A total of 21 cases of aldehyde dehydrogenase-2*2 ( ALDH2*2 ) , 19 cases of cytochrome p450 2 C19*2 ( CYP2 C19*2 ) and 19 cases of CYP2C19*3 were analyzed with the established method, and the genotypes of ALDH2*2 were 10 cases of GG homozygote, 8 cases of GA heterozygote and 3 cases of AA homozygote; the genotypes of CYP2C19*2 were 9 cases of GG homozygote, 8 cases of GA heterozygote and 2 cases of AA homozygote;and the genotypes of CYP2C19*3 were 18 cases of GG homozygote and 1 case of GA heterozygote. These results were consistent with those by pyrosequencing. The established method was specific, simple, short time-consuming and low cost, and could be used for the detection of SNP genotyping with non-polluting in single closed tube.
3.Diagnosis of two cases from one family with Joubert syndrome caused by novel mutations of TCTN1 gene by whole exome sequencing.
Huanhuan WANG ; Wenting JIANG ; Mengyao DAI ; Bing XIAO ; Yan XU ; Yu SUN ; Yu LIU ; Xiaomin YING ; Yunlong SUN ; Wei WEI ; Xing JI
Chinese Journal of Medical Genetics 2019;36(7):686-689
OBJECTIVE:
To explore the pathogenesis of two fetuses from one family affected with Joubert syndrome (JS).
METHODS:
Whole exome sequencing was employed to screen potential mutations in both fetuses. Suspected mutations were verified by Sanger sequencing. Impact of intronic mutations on DNA transcription was validated by cDNA analysis.
RESULTS:
Two novel TCTN1 mutations, c.342-8A>G and c.1494+1G>A, were identified in exons 2 and 12, respectively.cDNA analysis confirmed the pathogenic nature of both mutations with interference of normal splicing resulting in production of truncated proteins.
CONCLUSION
The genetic etiology of the family affected with JS has been identified.Above findings have enriched the mutation spectrum of TCTN1gene and facilitated understanding of the genotype-phenotype correlation of JS.
Abnormalities, Multiple
;
diagnosis
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genetics
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Cerebellum
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abnormalities
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Eye Abnormalities
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diagnosis
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genetics
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Humans
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Kidney Diseases, Cystic
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diagnosis
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genetics
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Membrane Proteins
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genetics
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Mutation
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Pedigree
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Retina
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abnormalities
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Whole Exome Sequencing
4.Experimental measurement and modeling analysis of active and passive mechanical properties of arterial vessel wall.
Yundi FENG ; Hao WU ; Yunlong HUO
Journal of Biomedical Engineering 2020;37(6):939-947
Coronary artery diseases (CAD) have always been serious threats to human health. The measurement, constitutive modeling, and analysis of mechanical properties of the blood vessel wall can provide a tool for disease diagnosis, stent implantation, and artificial artery design. The vessel wall has both active and passive mechanical properties. The passive mechanical properties are mainly determined by elastic and collagen fibers, and the active mechanical properties are determined by the contraction of vascular smooth muscle cells (VSMC). Substantial studies have shown that, the two-layer model of the vessel wall can feature the mechanical properties well, and the circumferential, axial and radial strain and stress are of great significance in arterial wall mechanics. This study reviewed recent investigations of mechanical properties of the vessel wall. Challenges and opportunities in this area are discussed relevant to the clinical treatment of coronary artery diseases.
Biomechanical Phenomena
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Coronary Vessels
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Humans
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Models, Cardiovascular
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Myocytes, Smooth Muscle
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Stress, Mechanical
5.Genotypic and phenotypic analysis of a patient with de novo partial monosomy 18p and partial trisomy 18q.
Bing XIAO ; Xing JI ; Hui YE ; Yu LIU ; Ying CAO ; Yunlong SUN ; Wei WEI ; Wenjuan QIU
Chinese Journal of Medical Genetics 2019;36(5):484-487
OBJECTIVE:
To explore the genetic cause for a patient with intellectual disability, short stature and multiple congenital anomalies, and to correlate the result with the clinical phenotype.
METHODS:
Routine karyotyping analysis was carried out on GTG-banded metaphase chromosomes. Single nucleotide polymorphism (SNP) microarray was used to detect microdeletions or microduplications in the patient. Fluorescence in situ hybridization (FISH) was used to ascertain the origin of aberrant chromosomes.
RESULTS:
The karyotype of the patient was 46,XY,der(18), while both of his parents had a normal karyotype. SNP array identified a 1.23 Mb deletion at 18p11.32-pter (chr18: 136 227-1 370 501, hg19) and a 33.76 Mb duplication at 18q21.1-qter (chr18: 44 250 359-78 013 728, hg19) in the patient. Above finding was confirmed by dual-color FISH with one color for 18p and another for 18q. The patient presented with some common features of 18p deletion and 18q duplication including intellectual disability and growth retardation, in addition with some features of 18p deletion including pectus excavatum, short stature and growth hormone (GH) deficiency. The patient showed progressive improvement of stature with GH therapy. Comparison of patients with previously reported dup(18q)+del(18p) recombinations suggested that, even for patients with similar breakpoints, their phenotypes have ranged from normal to severe and there were no consistent findings.
CONCLUSION
As aberrations involving double chromosomal segments often result in phenotypic variability, it has been difficult to correlate the genotype of our patient with his phenotype.
Abnormalities, Multiple
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Chromosome Deletion
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Chromosomes, Human, Pair 18
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Genotype
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Humans
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In Situ Hybridization, Fluorescence
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Karyotyping
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Monosomy
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Phenotype
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Trisomy
6.Current progress of computational modeling for guiding clinical atrial fibrillation ablation.
Zhenghong WU ; Yunlong LIU ; Lv TONG ; Diandian DONG ; Dongdong DENG ; Ling XIA
Journal of Zhejiang University. Science. B 2021;22(10):805-817
Atrial fibrillation (AF) is one of the most common arrhythmias, associated with high morbidity, mortality, and healthcare costs, and it places a significant burden on both individuals and society. Anti-arrhythmic drugs are the most commonly used strategy for treating AF. However, drug therapy faces challenges because of its limited efficacy and potential side effects. Catheter ablation is widely used as an alternative treatment for AF. Nevertheless, because the mechanism of AF is not fully understood, the recurrence rate after ablation remains high. In addition, the outcomes of ablation can vary significantly between medical institutions and patients, especially for persistent AF. Therefore, the issue of which ablation strategy is optimal is still far from settled. Computational modeling has the advantages of repeatable operation, low cost, freedom from risk, and complete control, and is a useful tool for not only predicting the results of different ablation strategies on the same model but also finding optimal personalized ablation targets for clinical reference and even guidance. This review summarizes three-dimensional computational modeling simulations of catheter ablation for AF, from the early-stage attempts such as Maze III or circumferential pulmonary vein isolation to the latest advances based on personalized substrate-guided ablation. Finally, we summarize current developments and challenges and provide our perspectives and suggestions for future directions.
7.Results analysis of unexpected antibody screening for blood donors and cost comparison of two detection modes
Liqin HUANG ; Lifeng WU ; Tong LI ; Ran LI ; Heng LIU ; Dandan DU ; Yunlong CHEN ; Xuezheng ZENG ; Jinfeng ZENG
Chinese Journal of Blood Transfusion 2022;35(3):324-327
【Objective】 To establish a routine screening method for unexpected antibodies of blood donors, analyze the results of centralized screening for unexpected antibody of blood donors in the blood center, and compare the cost of centralized and decentralized screening modes. 【Methods】 A total of 35 591 blood donors were screened for unexpected antibodies from March 31, 2021 to July 31, 2021, using microcolumn gel method. Unexpected antibody screening reactive samples were further confirmed by the Transfusion Research Institute of Shenzhen Blood Center, and the demographic characteristics were further determined through the analysis of unexpected antibody positive population. The direct cost and indirect cost of centralized and decentralized unexpected antibody screening mode were compared. 【Results】 Forty unexpected antibody positive samples were confirmed in Shenzhen, with the positive rate at 0.11%(40/35 591), among which MNS, Rh and Lewis system accounted for 35% (14/40), 32.5% (13/40) and 17.5% (7/40), respectively. Males and females accounted for 45% (18/40) and 55% (22/40), respectively (P<0.01). No significant difference was noticed by age and repeated-donor or not (P>0.05). Unexpected antibody screening in a centralized way saved about 1.16 million yuan per year. 【Conclusion】 It is necessary to carry out unexpected antibody screening for all blood donors, and centralized screening is more economical than decentralized screening.
8.Identifying potential anti-COVID-19 pharmacological components of traditional Chinese medicine Lianhuaqingwen capsule based on human exposure and ACE2 biochromatography screening.
Xiaofei CHEN ; Yunlong WU ; Chun CHEN ; Yanqiu GU ; Chunyan ZHU ; Suping WANG ; Jiayun CHEN ; Lei ZHANG ; Lei LV ; Guoqing ZHANG ; Yongfang YUAN ; Yifeng CHAI ; Mingshe ZHU ; Caisheng WU
Acta Pharmaceutica Sinica B 2021;11(1):222-236
Lianhuaqingwen (LHQW) capsule, a herb medicine product, has been clinically proved to be effective in coronavirus disease 2019 (COVID-19) pneumonia treatment. However, human exposure to LHQW components and their pharmacological effects remain largely unknown. Hence, this study aimed to determine human exposure to LHQW components and their anti-COVID-19 pharmacological activities. Analysis of LHQW component profiles in human plasma and urine after repeated therapeutic dosing was conducted using a combination of HRMS and an untargeted data-mining approach, leading to detection of 132 LHQW prototype and metabolite components, which were absorbed
9. The influence of cognition on suicidal ideation of major depressive patients
Wei LI ; Chengjun JI ; Fude YANG ; Qingtao BIAN ; Yongsheng TONG ; Zhiren WANG ; Kebing YANG ; Haipeng CAI ; Xin WANG ; Yunlong TAN
Chinese Journal of Behavioral Medicine and Brain Science 2019;28(9):827-831
Objective:
To analyze the influence of cognition on suicidal ideation in patients with major depressive disorder.
Methods:
A total of 108 inpatients with major depressive disorder from January 2017 to September 2018 in Beijing Huilongguan Hospital were enrolled.They were divided into suicidal ideation group (
10.Clinical practice guideline for body composition assessment based on upper abdominal magnetic resonance images annotated using artificial intelligence.
Han LV ; Mengyi LI ; Zhenchang WANG ; Dawei YANG ; Hui XU ; Juan LI ; Yang LIU ; Di CAO ; Yawen LIU ; Xinru WU ; He JIN ; Peng ZHANG ; Liqin ZHAO ; Rixing BAI ; Yunlong YUE ; Bin LI ; Nengwei ZHANG ; Mingzhu ZOU ; Jinghai SONG ; Weibin YU ; Pin ZHANG ; Weijun TANG ; Qiyuan YAO ; Liheng LIU ; Hui YANG ; Zhenghan YANG ; Zhongtao ZHANG
Chinese Medical Journal 2022;135(6):631-633