ObjectiveTo discuss the clinical efficacy of distal medial arm perforator flaps.MethodsAccording to the basic anatomy of distal medial arm perforator flaps,the distal medial arm perforator flaps were designed for repairing skin defects around elbow joints and at upper part of forearms in 15 cases.ResultsAll flaps were survived and first intention of wounds was obtained.At 3-36 months of follow-up,flap shape and elbow joint function revealed satisfactory recovery.Conclusions With the constant anatomical position,good blood supply,safe surgical approaches and cryptic donor site,the distal medial arm perforator flap is an alternative to repair the skin defects around elbow joints and in proximal forearms.

Objective To explore the clinical application of perforating branch flap of medial vastus muscle. Methods Perforating branch flap (muscle branch) of medial vastus muscle was designed by using the surface projection of the medial vastus muscle artery as flap anxial line and the given point of direct cutaneous artery as flap center to repair skin and soft tissue defects of the knee in seven patients.Results All flaps survived well with primary healing in all patients, with one stage healing. After a follow-up for 1-18 months, all flaps turned out to be with good texture and satisfactory appearance and function of the flaps. Conclusion The surgery of perforating branch flap of medial vastus muscle is simple,safe and easy handling and provides a new feasible surgical procedure to repair skin and soft tissue defects of medial femur and around the knee.

Objective To discuss clinical application of descending genicular artery perforator flap.Methods According to the anatomic features of direction,branches and anastomosis of the descending genicular artery,the descending genicular genicular artery perforator flap at medial superior aspect of knee joint was designed to reconstruct the soft tissue defects at the anterior medial 1/3 of the calf and the anterior medial part and popliteal fossa of the knee,with the axis based on the anterior border of the Sartorius and with the pivot point on the site where the cutaneous branches from the superior medial genicular artery pierced out within the triangle concave surface bounded by the vastus medialis,the tendon of adductor magnus and the condylus medialis.Results All flaps survived well in five patients,with primary healing.After a follow-up of 1-12 months,all flaps turned out to be with good texture,near-normal color and good appearance.Conclusion With a constant anatomic location,excellent blood supply and easy surgical procedure,the descending genicular artery perforator flap is one of feasible ways for repair of soft tissue defects around the knee.

BACKGROUND:The anesthesia process is required strict in establishing rabbit models of ileal neobladder. It is required for cleaning operation field, quick postoperative recovery, and the choice of anesthetics and anesthesia does not influence smooth muscle peristalsis. OBJECTIVE:To compare the anesthetic effects between urethane and chloral hydrate in rabbits with ileal neobladder. METHODS:Eighteen rabbits were randomly assigned to urethane and chloral hydrate groups, and were respectively anesthetized with 20% urethane 5 mL/kg and 10% chloral hydrate 2 mL/kg through the ear vein. Double ureter was dissociated at 1 cm outside original bladder, and 15 cm-intestine obtained from 10 cm of ileocecal junction was implanted so as to establish models of ileal neobladder. RESULTS AND CONCLUSION:Heart rates were significantly lower compared with these before anesthesia in al cases (P≤ 0.05), but no differences were detected between two groups (P > 0.05). Compared with the chloral hydrate group, the maintain time and recovery time were longer and the mortality rate was higher in the urethane group. Above data indicated that chloral hydrate is a safe long-acting anesthetic in establishing rabbit models of ileal neobladder.

A novel and highly sensitive voltammetric enzyme-linked immunosensor was developed based on tyramine) oxidation deposition. It was shown that gold nano-particles(colloid Au) could be used as a platform to immobilize antibodies by adsorption. By a sandwich immunossary format with goat-anti-human IgG labled Horseradish peroxidase(HRP) as the second antibody and catalytic amplification by biotin-tyramine, the immunosensor′s) catalytic ability to hydrogen peroxide increased nearly 20 times), the sensor exhibitd a linear response to human IgG in the concentration range from 1.5 μg/L-22 mg/L, and the detection limit was 0.1 μg/L), the regression equation could be expressed as Δi_p(μA) =2.8859c(mg/L)+17.152 with a correlation) coefficient of 0.9872. The immunosensor can be used to quantitatively determine hIgG in the sample) of human serum).

This paper introduced the management process of acceptance of acute myocardial infarction patients with suspected COVID-19 by Cardiac Catheterization Laboratory which carried out general management in many aspects, such as system and process optimization, whole quality prevention and control management as well as special disinfection and isolation. This paper aimed at providing reference for medical staff to ensure the rapid and highly efficient treatment for patient and zero infection in medical staff, and providing basis for clinical nursing, prevention and control work.

Objective To explore the clinical efficacy of multi-channel functional electrical stimulation cycling ergometer for children with spastic cerebral palsy.Methods A total of 60 chil-dren with rehabilitation treatment for spastic cerebral palsy in Outpatient Department were selected and randomly divided into observation group and control group,with 30 cases in each group.The control group received conventional rehabilitation treatments such as exercise therapy,occupational therapy and Chinese medicine massage,while the observation group received multi-channel functional electrical stimulation cycling ergometer training on the basis of the conventional rehabilitation treat-ments.Before treatment and 12 weeks after treatment,the Gross Motor Function Measure(GMFM-88),Peabody Developmental Measure Scale-Gross Motor(PDMS-GM),Berg Balance Scale(BBS),and Activities of Daily Living(ADL)were used to assess the gross motor function,balance function,and activities of daily living of the children in both groups.Results Before treatment,there were no sig-nificant differences in GMFM-88(D area,E area score,and total score),PDMS-GM(posture score,mobility score,and manipulation score),BBS,and ADL scores between the two groups(P＞0.05).After treatment,the GMFM-88(D area,E area score and total score),PDMS-GM(posture score,mobility score and manipulation score),BBS and ADL scores in both groups improved significantly compared with those before treatment,and all the scores in the observation group were significantly better than those in the control group(P＜0.05).Conclusion Multi-channel functional electrical stimulation cycling ergometer can effectively improve the gross motor function,balance function,and activities of daily living of children with spastic cerebral palsy.

Objective:To explore the treatment method and clinical effect of chronic diabetic wounds of lower limbs in elderly patients with comorbidities.Methods:The clinical data of elderly comorbid patients with chronic diabetic wounds of lower limbs admitted to Chongming Hospital Affiliated to Shanghai University of Medicine & Health Sciences from December 2019 to November 2022 were retrospectively analyzed. Multidisciplinary consultation and comprehensive evaluation were conducted for the patient before surgery and the indicators related to the underlying disease were also adjusted to the safe range for surgery. The wound secretions were taken in time for bacterial culture and drug sensitivity test, and the wound was treated with dressing change and other standardized treatments. According to the location, shape, size and depth of the lower limb wound defect, as well as the infection degree, duration of infection, and extent of surrounding skin damage, debridement, vacuum sealing drainage, tension-reduced suture, skin or flap transplantation, limb (toe) amputation and other method were selected for treatment. After surgery, the prevention and control of the comorbidities of the elderly and nursing management were continued, and the wound healing was observed. According to the evaluation criteria of flap swelling at grade 4 (increased from grade Ⅰ to grade Ⅳ), the swelling degree of the lower leg and dorsum of the foot was evaluated in the early stage (3 to 12 weeks) and the later stage (13 to 52 weeks). At the last follow-up, according to the evaluation criteria of the ankle joint wound repair, the curative effect was comprehensively evaluated (excellent, good, general, poor), and the satisfaction evaluation (satisfied, general, unsatisfied) was also conducted.Results:A total of 85 patients were included, of which 41 were males and 44 were females; the age ranged from 65 to 93 years, with a mean of 71.4 years. Comorbidities of the elderly include hypertension, coronary heart disease, cerebrovascular accident, renal insufficiency, diabetes, tumor, gout, senile dementia, senile neurasthenia, sleep disorders, malnutrition, epilepsy, urinary incontinence, delirium, depression, etc. The wound duration ranged from 6 months to 15 years. The depth of the wound reached muscle or bone, and the defect area ranged form 3.5 cm×2.5 cm to 22.0 cm×10.5 cm. All 85 patients had positive bacterial cultures of wound secretions. Drug sensitivity test results: 72 cases were sensitive, 10 cases were intermediate, and 3 cases were completely resistant. Clinical medication was based on the drug sensitivity test result and the principles of antibiotic use. For the 3 resistant cases, nutrition, support and other treatments to enhance immunity were given, and the local wound was dressed with 0.45%-0.55% iodophor. Among the 85 cases, 65 were treated with vacuum sealing drainage, 10 patients were treated with tension-reduced suture, 19 were treated with full-thickness skin graft, and 21 were treated with local flap transfer, 20 were repaired by perforator pedicle flap, and 15 underwent amputation (toe). The follow-up period was 6 to 36 months, with an average of 12.5 months. Wound healed by primary intention in 57 cases; delayed healing in 21 cases, wound healed after dressing change or surgical repair; wound not healed in 7 cases, wound completely healed after amputation (toe) or other surgical treatment. Evaluation of the degree of swelling in the lower leg and dorsum of the foot: 18 cases of grade Ⅰ, 47 cases of grade Ⅱ, 15 cases of grade Ⅲ, and 5 cases of grade Ⅳ in the early stage; 62 cases of grade Ⅰ, 18 cases of grade Ⅱ, and 5 cases of grade Ⅲ in the later stage. Comprehensive evaluation of the therapeutic effect: 23 cases were excellent, 45 cases were good, 16 cases were general, and 1 case was poor, with an excellent-good rate of 80%. Satisfaction evaluation: 78 cases were satisfied, 7 cases were general, and the satisfaction rate was 91.8%.Conclusion:Generalist clinical thinking and multidisciplinary collaborative model are important strategies for treating chronic diabetic wounds of lower limbs in elderly patients with comorbidities. By controlling infection with antibiotics and dressing changes, and using reconstructive surgery techniques to repair the wound, the wound healing rate can be improved and satisfactory treatment outcomes can be achieved.

Objective:To investigate clinical curative effect of multiple composite tissues replantation with microsurgical technique in single hand.Methods:From May 2008 to December 2021, the clinical data of patients who underwent multiple composite tissue replantation in single hand in Chongming Hospital Affiliated to Shanghai University of Medicine & Health Sciences and Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, were retrospectively analyzed. According to the severed site, anatomical level, the injury cause, the severity of injury, the size of severed tissue, and replantation conditions (such as blood vessels for anastomosis), the severed tissue was evaluated and carried out modified classification (type Ⅰ-Ⅳ) before the operation, and the surgical plan was designed. The severed composite tissue was debrided in emergency, the severed bone and muscle tissue were fixed, and the arteries, veins and nerves were anastomosed by microsurgical technique under a high-power microscope. The ratio of arteries to veins was 1∶1-1∶2. If enough arteries and veins could not be found, arteriovenous shunt could be used to form arterial venous flap or venous arterialized flap, or form artery flap by just artery anastomosis, or form venous flap by just vein anastomosis. Then skin tissue was sutured after recanalization. After the operation, the patients received specialized treatment and guided rehabilitation exercise. The wound healing of hand, skin color, skin quality, swelling degree, sensation, pain degree of joint, and activity function were followed up after the operation. At the last follow-up, the patient’s self-evaluation of the treatment effect was divided into three levels: satisfactory, average, and unsatisfactory. One chief physician and one doctor-in-charge were responsible for the comprehensive evaluation of curative effect, according to the assessment standard for upper limb function issued by Chinese Medical Association of Hand Surgery. 80-100 points were excellent, 60-79 points were good, 40-59 points were fair, and <40 points were poor.Results:A total of 94 severed composite tissues in 35 patients, including of 20 male patients and 15 female patients were enrolled. The average age was 36.4 years (range, 16-65 years). Fifty severed tissues in 18 cases were classified into type Ⅰ, 40 severed tissues in 15 cases type Ⅱ, 4 severed tissues in 2 cases type Ⅲ. Among the 35 cases, 1 case of thumb pulp severed tissue was necrotic at the edge (0.3 cm×0.5 cm) due to local contusion, 1 case of index finger lateral severed tissue was necrotic at the edge (0.3 cm×0.8 cm) due to local avulsion injury, and healed after dressing change, and the other replanted composite tissue survived. The patients were followed up for 3 to 65 months (average, 18.5 months). The wounds were completely healed, and the color, elasticity, and quality of the replanted tissue was close to the surrounding normal skin. There was no obvious swelling or atrophy deformity of the hands. According to the patients’ self-assessment, 31 cases were satisfied and 4 cases were average. Comprehensive evaluation of hand function and appearance: excellent in 23 cases, good in 9 cases, fair in 3 cases, excellent and good rate was about 91.4% (32/35).Conclusion:Though multiple composite tissues replantation with microsurgical technique in single hand is difficult, and vascular anastomosis technical requirement is high, once surgery succeeds, the function and appearance can get good recovery, and patient satisfaction is also relatively high.

Objective To explore the clinical efficacy of multi-channel functional electrical stimulation cycling ergometer for children with spastic cerebral palsy.Methods A total of 60 chil-dren with rehabilitation treatment for spastic cerebral palsy in Outpatient Department were selected and randomly divided into observation group and control group,with 30 cases in each group.The control group received conventional rehabilitation treatments such as exercise therapy,occupational therapy and Chinese medicine massage,while the observation group received multi-channel functional electrical stimulation cycling ergometer training on the basis of the conventional rehabilitation treat-ments.Before treatment and 12 weeks after treatment,the Gross Motor Function Measure(GMFM-88),Peabody Developmental Measure Scale-Gross Motor(PDMS-GM),Berg Balance Scale(BBS),and Activities of Daily Living(ADL)were used to assess the gross motor function,balance function,and activities of daily living of the children in both groups.Results Before treatment,there were no sig-nificant differences in GMFM-88(D area,E area score,and total score),PDMS-GM(posture score,mobility score,and manipulation score),BBS,and ADL scores between the two groups(P＞0.05).After treatment,the GMFM-88(D area,E area score and total score),PDMS-GM(posture score,mobility score and manipulation score),BBS and ADL scores in both groups improved significantly compared with those before treatment,and all the scores in the observation group were significantly better than those in the control group(P＜0.05).Conclusion Multi-channel functional electrical stimulation cycling ergometer can effectively improve the gross motor function,balance function,and activities of daily living of children with spastic cerebral palsy.