Objective The increasing evidences indicate that the inhalable particle matter (PM10) is responsible for respiratory and cardiovascular morbidity and mortality.Generation of superoxide anion,hydroxyl radical and apoptosis in HLF cells induced by PM10 were studied in this paper to present some data for elucidating the mechanism of effects of PM10.Methods The superoxide anion and hydroxyl radical were detected respectively by cytochrome C assay and apoptosis inspected with Annexin V-FITC/PI double staining by flow cytometry.Results PM10 could generate the superoxide anion and hydroxyl radical,with significant dose-dependent manners,and the levels of the superoxide anion as well as hydroxyl radical were decreased by chitosan oligosaccharides (COS),with the significant protection effects (P

BACKGROUND:Recently, the role of mesenchymal stem cels in aplastic anemia has been widely explored. However, its underlying mechanism remains unclearly. OBJECTIVE: To study the effect of umbilical cord blood and bone marrow mesenchymal stem cels on hematopoietic support and secretory function of T lymphocytes in patients with aplastic anemia. METHODS: Cord blood and bone marrow samples from 48 cases of aplastic anemia and 48 healthy lying-in women to isolate mesenchymal stem cels using flow cytometry. Mesenchymal stem cels from the cord blood and bone marrow were respectively co-cultured with cord blood mononuclear cels to count burst forming units-erythroid and colony forming units-granulocyte/macrophage. Mesenchymal stem cels were co-cultured with T lymphocytes from aplastic anemia patients undergoing phytohemagglutinin stimulation, and ELISA was used to detect interleukin-2, interleukin-4 and interferon-γ levels secreted from T lymphocytes. RESULTS AND CONCLUSION:The number of burst forming units-erythroid and colony forming units-granulocyte/macrophage significantly increased in normal bone marrow or umbilical cord blood mesenchymal stem cels co-cultured with cord blood mononuclear cels (P < 0.05), but reduced remarkably in umbilical cord blood mesenchymal stem cels from aplastic anemia patients co-cultured with cord blood mononuclear cels (P < 0.05). Levels of interleukin-2, interleukin-4 and interferon-γ from T lymphocytes were inhibited significantly after co-culture with normal bone marrow mesenchymal stem cels compared with phytohemagglutinin-induced T lymphocytes (P < 0.05). There was a similar inhibitory effect after co-culture with normal umbilical cord blood mesenchymal stem cels. There was a significantly reduction in the capacity of inhibiting interleukin-2, interleukin-4 and interferon-γ levels from T lymphocytes after co-culture with bone marrow mesenchymal stem cels from aplastic anemia patients (P < 0.05). Aplastic anemia patients show some functional defects in their bone marrow mesenchymal stem cels that have a weaker inhibitory role than normal bone marrow or umbilical cord blood mesenchymal stem cels in the hematopoietic support and secretory function of T lymphocytes. These findings indicate that mesenchymal stem cels from aplastic anemia patients can influence the pathological progress through weakening hematopoietic support and secretory function of T lymphocytes. Cite this article:Li GC, Song YP, Zhang YJ, Li G, Wang H, Xie J.Effects of mesenchymal stem cels on hematopoietic support and secretory function of T lymphocytes in patients with aplastic anemia. Zhongguo Zuzhi Gongcheng Yanjiu. 2016;20(1):107-112.

Objective Toinvestigatetheeffectofmicroribonucleicacid(miRNA)-155ontwo subtypesofregulatoryTcell(Treg):inducedTreg(iTreg)andnaturalTreg(nTreg).Methods NaveT cells and nTreg were isolated from peripheral blood mononuclear cell (PBMC)of healthy donors by magnetic cell sorting. Cells were divided into 3 groups during culture,including control group (nave T cells were cultured with the presence of interleukin-2 ),iTreg group (nave T cells were cultured with the presence of interleukin-2 and transforming growth factor-β)and nTreg group(nTreg cells was cultured with interleukin-2).Each group was divided into 3 subgroups (none,scramble or miRNA-155 antagomir subgroup,3 wells in each subgroup). Expression level of miRNA-155 gene of none subgroup in 3 groups was detected by low density chip analysis method. The levels of surface marker CD25,Foxp3,CD127 of each subgroup in 3 groups were detected by flow cytometry. The percentage of CD4 +CD25 +Foxp3 +SOCS1 +Treg and suppressive function of Tregofeachsubgroupin3groupswerealsodetectedbyflowcytometry.Results Comparedwithcontrolgroup and iTreg group,the expression level of miRNA-155 was significantly lower and SOCS1 was significantly higher in nTreg group (all in P<0.05 ). After the addition of miRNA-155 antagomir,no significant change was observed in the important surface markers of Treg like Foxp3,CD25,CD127. Compared with control group and iTreg group,the expression of SOCS1 in nTreg group increased significantly (both in P <0.05 ). The expression level of miRNA-155 of none subgroup in iTreg group was lower. The expression of SOCS1 increased after the miRNA-155 was inhibited by antagomir (miRNA-155 antagomir subgroup). In iTreg group,the suppressive function of Treg in miRNA-155 antagomir subgroup was higher than that in none subgroup at the ratioof1∶8,1∶16and1∶32(allinP<0.05).Conclusions AntagonismofmiRNA-155invitrohasno significant effect on the suppression function of nTreg,but can increase the SOCS1 expression level and suppression in vitro of iTreg.

Objective: To compare the evaluation effects of abdominal enhanced computed tomography (CT) coronal imaging versus three-dimensional (3D) vascular reconstruction for critical blood vessels in right colon cancer. Methods: The retrospective and descriptive study was conducted. The clinicopathological data of 50 patients with right colon cancer who were admitted to Changhai Hospital Affiliated to Naval Medical University from January to September in 2018 were collected. There were 33 males and 17 females, aged from 33 to 86 years, with an average age of 63 years. All the 50 patients underwent abdominal multi-slice CT examination on the same CT equipment. The CT examination data were analyzed by two-dimensional (2D) coronal imaging and 3D vascular reconstruction. Observation indicators: (1) anatomical type of Henle trunk; (2) the length of Henle trunk and surgical trunk; (3) the positional relationship between ileocolic vein (ICV) and ileocolic artery (ICA). Measurement data with normal distribution were represented as Mean±SD, and count data were represented as absolute numbers. Kappa coefficients were used to measure the consistency between anatomical types of Henle trunk on 2D coronal images and on 3D vascular reconstructed images. Pearson coefficients were used to evaluate the correlation between the length of Henle trunk and surgical trunk on 2D coronal images and on 3D vascular reconstructed images. Bland-Altman method was used to assess the consistency between the length of Henle trunk and surgical trunk on 2D coronal images and on 3D vascular reconstructed images. Results: (1) Anatomical type of Henle trunk: on the 2D coronal images, 43 of 50 patients had the Henle trunk and 7 had no Henle trunk. On the 3D vascular reconstructed images, 44 of 50 patients had the Henle trunk and 6 had no Henle trunk. There were 2, 21, 17, 3 patients classified as type 0, Ⅰ, Ⅱ, Ⅲ of Henle trunk on the 2D coronal images of 43 patients. There were 6, 19, 16, 3 patients classified as type 0, Ⅰ, Ⅱ, Ⅲ of Henle trunk on the 3D vascular reconstructed images of 44 patients. Six patients with no Henle trunk, 2 in type 0, 18 in type Ⅰ, 15 in type Ⅱ, and 3 in type Ⅲ had the same anatomical type of Henle trunk on the 2D and 3D images. The consistency between anatomic types of Henle trunk on 2D coronal images and on 3D vascular reconstructed images was high (κ=0.830, 95% confidence interval: 0.705-0.956, P<0.05). (2) The length of Henle trunk and surgical trunk: on the 2D coronal images, 43 of 50 patients had the length of Henle trunk as (10±5)mm, and 42 of 50 patients had the length of surgical trunk as (34±12)mm. On the 3D vascular reconstructed images, 44 of 50 patients had the length of Henle trunk as (9±5)mm, and 43 of 50 patients had the length of surgical truck as (35±12)mm. The correlation between the length of Henle trunk and surgical trunk on 2D coronal images and on 3D vascular reconstructed images was positive (r=0.872, 0.979, P<0.05). Bland-Altman plot showed a high consistency between the length of Henle trunk and surgical trunk on 2D coronal images and on 3D vascular reconstructed images (P<0.05). (3) The positional relationship between ICV and ICA: on the 2D coronal images, 24 of 50 patients had anterior crossing between ICV and ICA, 26 had posterior crossing between ICV and ICA. On the 3D vascular reconstructed images, 24 of 50 patients had anterior crossing between ICV and ICA, 26 had posterior crossing between ICV and ICA. There was a complete consistency in the positional relationship between ICV and ICA on the 2D coronal images and on 3D vascular reconstructed images. Conclusion Abdominal enhanced CT coronal imaging and 3D vascular reconstruction have the similar evaluation effects for position of critical blood vessels in right colon cancer, with a good consistency.