1.MicroRNA and Viral Infection.
Fulin ZHAO ; Yuning LI ; Zhaojun DUAN
Chinese Journal of Virology 2015;31(2):187-191
MicroRNA (miRNA) are small non-coding molecules of ribonucleic acid. They are about 22 nucleotides in length, single-stranded, and mediate post-translational regulation by the repression or degradation of messenger RNA(mRNA). miRNA play a key part in the proliferation, differentiation and death of cells. Viral infection is one of the most common causes of human disease. Some studies have found that miRNA has a very close relationship with viral infection, which has an effect on viral replication, the immune response and antiviral immunity. Use of miRNA may become the cornerstone of new methods for the diagnosis and treatment of viral infection. This article summarizes the progress of research into miRNA and viral infection.
Animals
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Humans
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MicroRNAs
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genetics
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metabolism
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Virus Diseases
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genetics
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metabolism
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virology
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Virus Physiological Phenomena
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Virus Replication
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Viruses
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genetics
2.Temperature Measurement for Validation of Clinical Moist Sterilizers
Qing ZHANG ; Jintao ZHANG ; Hong GAO ; Yuning DUAN ; Ping QIU
Chinese Journal of Nosocomiology 1994;0(04):-
OBJECTIVE To report the investigation on validating clinical moist sterilizer by applying wireless thermo-loggers in accordance with the advanced Euro and British standards.METHODS The validation had been implemented with microbiological tests and thermometric tests to measure the temperature distribution of the chamber of sterilizer,the temperature and time relation inside the tested package of small and challenge loads,the pressure of the chamber and the temperature beside the drainage.RESULTS The measurements presented the relation between temperature and time measurements of the spots inside the chamber of the tested sterilization loads.CONCLUSIONS The measurement results indicate directly the operation condition and the temperature-time relation inside sterilization loads.These measurements are helpful for controlling sterilization quality.The results of microbiological tests are negative,which are consistent with that of thermomeric tests.
3.Discovery of human bocavirus 4 in children with acute gastroenteritis in Lanzhou, China.
Jingyao XIANG ; Xin MA ; Yanqing GUO ; Zhaojun DUAN ; Yuning LI ; Dandi LI
Chinese Journal of Virology 2015;31(1):42-45
We studied the epidemiological characteristics of human bocavirus 4 (HBoV4) in children with a- cute gastroenteritis in Lanzhou (China). A total of 331 stool specimens were collected from children aged < 5 years with acute diarrhea at the First Hospital of Lanzhou University between July 2012 and June 2013. Specimens of HBoV were identified by nested polymerase chain reaction assays. Compared with related sequences in GenBank, the HBoV-positive strain isolated in the present study was,quite surprisingly, a rare genotype named HBoV4. This strain was a typical HBoV4,with high levels of nucleotide and amino acid homology to the Thailand strain, JQ267789 (98.9% and 98.7%, respectively), and the USA strain, GQ506568 (97.6% and 97.4%, respectively). This is the first report of HBoV4 as the causative agent for acute gastroenteritis in pediatric patients in China. This strain is one of two genotypes of HBoV that are currently circulating.
Child, Preschool
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China
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Feces
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virology
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Female
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Gastroenteritis
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virology
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Human bocavirus
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classification
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genetics
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isolation & purification
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Humans
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Infant
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Male
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Molecular Sequence Data
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Parvoviridae Infections
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virology
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Phylogeny
4.Role of prostaglandin E2 in regulation of renin secretion at juxtaglomerular apparatus
Limeng CHEN ; Yuning HUANG ; Yan QIN ; Dongyan LIU ; Yan LI ; Lin DUAN
Chinese Journal of Nephrology 2009;25(3):217-221
ObjectiveTo investigate the effect and mechanism of prostaglandin E2 (PGE2) in renin regulation at the juxtaglomerular apparatus (JGA). MethodsMacula densa cell line (MMDD1) was cultured on the special filter. In the medium on the apical lateral of the cells, low concentration of sodium chloride, chloride and different doses of angiotensin Ⅱ (Ang Ⅱ) were used to stimulate the PGE2 secretion. The PGE2 concentration was tested by ELISA. In the animal experiment, the response of plasma renin activity (PRA) to acute intraperitoneal administration of captopril (30 mg/kg) was determined, in conscious wild-type (WT) and cyclooxygenase COX-2-/- mice on C57BL/6 genetic backgrounds. PRA was measured in plasma obtained by tail vein puncture. Different concentrations of PGE2 were used to stimulate the renin secretion of primary cultured JGA cells from COX-2-/- mice and wild type mice. In specific Gsα gene delete mice (low renin producing mice), 24 h urine was collected to test the concentration of PGE2. The COX-2 mRNA and protein of the kidney cortex were observed by real-time PCR and immunohistochemicul staining. ResultsLow chloride could stimulate the PGE2 secretion both at the apical and basement of the macula densa cells. In COX-2-/- mice, the base PRA and were obviously lower than wild type mice. Captopril could stimulate the PRA of (COX)-2-/- mice increasing 32.8 times. But Ang Ⅱ had no effect on PGE2 secretion in macula densa cells. In primary cultured JGA cells, the decreasing renin seretion was partly recovered by PGE2 in cells from COX-2-/- mice. In low renin producing mice, the expression of COX-2 mRNA in the kidney cortex increased by (8.07±1.08) times (n=6, P=0.0022). The COX-2 protein of the kidney cortex and the urine PGE2 increased by several times. ConclusionsLow chloride is the primary stimulation messenger of PGE2 secretion in macula densa cells. The PRA in COX-2-/- mice can be stimulated by angiotensin converting enzyme inhibitor, but the Ang Ⅱ has no direct effect on macula densa cells. When renin production is abolished in JGA cells (Gsα delete mice), COX-2 mRNA and protein up-regulation is observed in kidney cortex and macula densa. PGE2 plays an important role in regulation of renin secretion and renin release in JGA by precise feedback mechanism.
5.Inactivation of EV71 by Exposure to Heat and Ultraviolet Light.
Jing XIE ; Dandi LI ; Guangcheng XIE ; Yaqian HU ; Qing ZHANG ; Xiangyu KONG ; Nijun GUO ; Yuning LI ; Zhaojun DUAN
Chinese Journal of Virology 2015;31(5):500-506
Enterovirus 71 (EV71) is a major agent of hand, foot and mouth disease that can cause a severe burden of disease to children. To identify an effective method for the control and prevention of EV71, we studied the effect of exposure to heat and ultraviolet (UV) light upon EV71 inactivation. We found that exposure to 50 degrees C could not inactivate the infectivity of EV71. However, exposure to 60 degrees C and 70 degrees C could inactivate EV71 effectively. EV71 could be inactivated after exposure to UV light at a distance between the sample and a lamp of 30 cm for 30 min or 60 min because viral genomic RNA was destroyed. However, fetal bovine serum (FBS) could attenuate the inactivation proffered by heat and UV light. Attenuation effects of FBS were correlated positively with FBS concentration. Hence, EV71 can be inactivated by exposure to heat and UV light, and our results could provide guidance on prevention of the spread of EV71.
Disinfection
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instrumentation
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methods
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Enterovirus A, Human
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genetics
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physiology
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radiation effects
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Enterovirus Infections
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virology
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Hot Temperature
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Humans
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Ultraviolet Rays
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Virus Inactivation
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radiation effects