25%, TCI of vecuronium was started at a target blood concentration of 0.3 ?g.ml-1 .Vecuronium was administrated by a specific TCI system incorporating the pharmacokinetic parameter set of Ruppy. Vecuronium target blood concentration was titrated to maintain T1/T2. at 25% .Results The average degree of NMB was 14.0%?2.2% in control group and 22.9%? 1.8% in TCI group. The difference was statistically significant ( P

ObjectiveTo discuss the effects and security o f intra-anmiotic administration of pulmonary surfactant for neonatal respiratory distress syndrome prophylaxis.MethodsForty-five cases of pr eterm delivery with immature fetal lungs were divided into two groups. Fifteen w omen of the study group were administered one dose of pulmonary surfactant into the amniotic cavity and delivered in several hours. Nothing was injected into th e amniotic cavity of the 30 women in the control group. The proportion of NRDS, mortality and the time in hospital were analyzed to make clear whether there wer e any differences between the two groups.ResulstsThere was n o difference between the two groups for neonatal Apgar score. Foam test demonstr ated that the proportion of lung maturity of the neonates was:56.3%(9/16)in the study group, 13.3%(4/30)in the control group,(P

Objective To study the effect of SARS during pregnancy on the pregnant outcome. Methods Six pregnant women who suffered from SARS in the second or third trimester were observed. The fetuses and infants were followed up. SARS specific antibody and coronavirus RNA were detected in blood and pharyngeal secretion samples for both mothers and their infants. Results Among the 6 cases,2 were twins. Two cases were preterm birth,3 were fetal distress. One fetal death in one twin,the other fetus complicated with fetal growth restriction. None of the 7 neonates had asphyxia or any signs of SARS.SARS coronavirus IgG was positive in 7 mothers and 4 infants. SARS specific IgM and coronavirus RNA were negative in all 7 newborns. Physical and mental development in 3 premature infants was slightly delayed while normal in the 4 full-term infants. Conclusions SARS during pregnancy may have some harmful effects on the perinatal outcome. No intrauterine transmission of coronavirus has been found.

Dexmedetomidine (Dex) is a novel and highly selective o2-adrenoceptor agonist,it has anxiolytic,antisympathetic and certain analgesic effects,its sedative effect can lead to mimic natural sleep.Dex is widely applied in mechanical ventilation,invasive procedures and perianesthesia with other drugs by intravenous route.Recent basic and clinical researches show that Dex can be applied safely and effectively with local anesthetics in regional block,to improve anesthetic effect,promote stable hemodynamics and optimize postoperative analgesia.

Objective To study the relationship between osteoporotic hip fracture (OHF) and polymorphism of cytochrome P450c19 genes. Methods By using a group correlation analysis, we made a case-control study of seven single nucleotide polymorphism (SNP) sites groupinge and haplotype in 400 OHF patients from Shaanxi Guanzhong population and 400 normal samples as controls. Results A total of 700 samples were successfully genotyped. Correlation analysis identified three blocks and selected seven tag SNPs. According to the variance analysis, rs7167343, rs8031463, and polymorphism of haplotype 3 were associated with hip fracture. Conclusion Cytochrome P450c19 genes have association with the incidence of osteoporotic fracture in the Han population we studied from Shaanxi Guanzhong region. Therefore, cytochrome P450c19 genes may affect the incidence of osteoporotic hip fracture in the Han population from Guanzhong region, Shaanxi Province.

Objective To investigate the effects of autoantibodies (β1-AA) against second extra-cellular loop of the β1-adrenergic receptor (β1-AR-ECⅡ) in sera of patients with dilated cardiomyopathy (DCM) on proliferation of rat CD4+T lymphocytes.Methods β1-AA in the sera of patients with DCM was purified by affinity chromatography .CD4+T lymphocytes were isolated by immunomagnetic microbeads from peripheral blood mononuclear cells of rats and its positive rate was detected by flow cytometry .CCK-8 meth-od was used to detect the proliferation of CD 4+T lymphocytes and flow cytometry was performed to measure the ratio of CD4+/CD8+T lymphocyte .Results The purity of isolated rat CD 4+T lymphocytes by immu-nomagnetic microbeads reached 97.7%.The proliferation of CD4+T lymphocytes stimulated by CD3/CD28 was inhibited by β1-AA in a concentration-dependent manner .However , IgG antibodies extracted from sera of healthy controls did not suppress lymphocyte proliferation (P>0.05).The suppression effect of β1-AA was inhibited after binding to antigenic peptides corresponding to β1-AR-ECⅡ and was completely blocked by metoprolol, a specific antagonist of β1-adrenergic receptor (β1-AR).In addition,β1-AA had no effects on the ratio of CD4+/CD8+T lymphocyte .Conclusion β1-AA isolated from DCM patients suppresses the pro-liferation of CD4+T lymphocytes through β1-AR pathway , which indicates that β1-AA can directly reduce the number of T lymphocytes and impair the function of T lymphocytes , resulting in immune system disorders and the development of DCM .

Objective To establish the model and software for quality assessment of fetal nuchal translucency ultrasound image using computer image recognition technology.Methods The proposed approach firstly divided the input image into four sub-image blocks:the nasal bone(NB) area,the nuchal translucency (NT) area,the midbrain area,and the jaw and chest area.For each sub-image block,the algorithm compared the image block with the corresponding area of the standard training image set,and then determined whether the current image block was the qualified one using the the Gabor feature and Bayesian decision.The input ultrasound image was determined to be qualified only if it had four qualified sub-image blocks.Results The difference between our automatic method and the manual screening by experts wasting small,the method obtained Kappa =0.795 and P ＜0.001.Moreover,the efficiency of our method was much higher than the manual screening method.Conclusions Image recognition technology can effectively assist the sonographer to assess the quality of fetal NT of ultrasound image.The proposed approach can reduce the subjectivity and randomness of the manual evaluation of NT image.

AIM: To investigate the effect of Thymosin ? 1 on the development and matutation of thymocytes. METHODS: The proportion of CD4+CD8+ thymocytes and the expression of smoothened (Smo) of the hedgehog (Hh)-signaling in CD4-CD8-thymocytes were examined to observe the effect of thymosin ? 1 on thymocyte development and matutation. RESULTS: Flowcytometric analysis showed that thymosin ? 1 showed activity at a low dose of 30 ?g/kg, and 30 ?g/kg thymosin ? 1 accelerated the replenishment and maturation of thymocytes according to the expression of Smo of the Hh-signaling in CD4-CD8-thymocytes, the potent negative regulator of proliferative responses. CONCLUSION: Thymosin ? 1 can accelerate thymocyte development from CD4-CD8- to CD4+CD8+.

Objective To develop a LC-MS/MS method for the determination of inosine in human plasma and to apply it in the isoprinosine pharmacokinetic researches .Methods With adefovir as the internal standard ,methanol -10 mmol/L ammonium ace-tate(15 :85 ,v/v) was adopted as the mobile phase .The chromatographic column was the Agilent SB-C18 column(5 μm ,150 mm × 4 .6 mm I .D .) .Electrospray ionization(ESI) source was applied and the detection was conducted by the multiple-reaction monito-ring(MRM) mode .The ion reactions for the quantitative analysis were m/z 135 .0-267 .3(inosine) and m/z 134 .1-272 .0(adefo-vir) .Results The linear range of plasma inosine was 10-3 000 ng/mL .The lower detection limit was 10 ng/mL .The inter-and in-tra-day precisions(RSD) were less than 5% .The average recovery rate was above 90% without the matrix effect .Conclusion This method has the strong specificity ,convenience in treating the sample and high sensitivity ,and is suitable for the inosine pharmacokinetic re-searches .

Objective To explore the relationship between HLA?B allele polymorphisms and nasopharyngeal carcinoma ( NPC) in Xinjiang, China and its clinical significance. Methods A total of 226 patients were assigned to NPC group, while 207 healthy volunteers were assigned to control group. PCR amplification with sequence?specific primers was used to determine HLA?B alleles. Comparison of HLA?B allele frequency between the above two groups, between Han and Uygur populations, and between patients with various clinical characteristics of NPC was made by chi?square test. The Kaplan?Meier method was used to calculate the survival rates and the log?rank univariate analysis was used to explore the relationship between survival rates and HLA?B allele frequency. Results In all the subjects or Han population alone, the allele frequency of HLA?B?46 in the NPC group was significantly higher than that in the control group ( P=0. 000;P=0. 000 ) . In Uygur population, however, there was no significant difference in the allele frequency of HLA?B?46 between the NPC group and the control group (P>0. 05). In the patients with NPC, those less than 30 years old had a significantly higher allele frequency of HLA?B?44 than those no less than 30 years old (P=0. 029);those with differentiated non?keratinizing carcinoma had a significantly higher allele frequency of HLA?B?48 than those with undifferentiated non?keratinizing carcinoma ( P=0. 029);those with stage T1+T2 disease had a significantly higher allele frequency of HLA?B?48 than those with stage T3+T4 disease ( P=0. 029) . The 5?year overall survival, disease?free survival, distant metastasis?free survival, and local relapse?free survival rates had no relationship with the expression of HLA?B?46, HLA?B?44, or HLA?B?48 in NPC patients ( all P>0. 05) . Conclusions HLA?B?46 allele is probably a NPC susceptibility gene in Han population in Xinjiang. HLA?B?44 is probably associated with early age of onset, while HLA?B?48 is probably associated with the pathological type and T stage of NPC. Therefore, HLA?B alleles are probably associated with the development and progression of NPC.