Objective To explore the effects of experiential teaching mode in pediatrics of traditional Chinese medicine. Methods One hundred students were randomly divided into trial group and control group, 50 students in each group. Trial group used the experiential teaching mode, while students in the control group were taught with traditional teaching mode. Results The degree of interest in pediatrics of traditional Chinese medicine, communication with the children and the parents of the patients, and examination results in the trial group were superior to the control group (P<0.05). Conclusion The effects of experiential teaching mode in pediatrics of traditional Chinese medicine are satisfied, and worth popularizing in teaching.

Objective To distinguish two Bithynia species,Bithynia fuchsiana and Bithynia robusta collected from Heng County,Guangxi Zhuang Autonomous Region,by using morphological and DNA barcoding methods. Methods The adult B. fuchsiana and B. robusta were collected from the biotope such as rivers,ditches and ponds in Heng County of Guangxi Zhuang Autonomous Region,China. The two species specimens were identified by measuring shell morphological parameters,compar?ing the characters of the male reproductive system,and using the COI gene barcoding technique and building phylogenetic tree. Results B. fuchsiana and B. robusta were similar morphologically in the shell appearance;they had the similar snail height, snail width,shape and male reproductive structure. The DNA sequence analysis showed that the COI gene of the two Bithynia species had low sequence divergence with 11 variation sites among 22 sequences. The length of the COI gene segment was 517 bp and no insertion sites and deletion loci after sequence edited. All individuals of the two species gathered to one clade in the phylogenetic tree based on COI gene. Conclusion According to the evidence of morphology and COI gene coding sequence,B. fuchsiana and B. robusta from Heng County,Guangxi Zhuang Autonomous Region,are likely to be the same species.

Objective To investigate the cardioprotective effects of 3-nitropropionic acid (3-NPA) pretreatment against ischemia-reperfusion (I/R) injury. Methods Sixteen male Wistar rats weighing 200-250 g were randomly divided into 2 groups ( n = 8 each):(1) 3-NPA group received intraperitoneal 3-NPA 4 mg?kg-1 24 h before the animals were sacrificed and (2) control group received normal saline instead of 3-NPA. The animals were sacrificed and the hearts were immediately removed and mounted on Langendorff apparatus and perfused with K-H solution saturated with 95% O2 and 5% CO2 at 37℃ . After being perfused for 30 min the hearts were subjected to 30 min global ischemia by suspension of perfusion followed by 60 min reperfusion. The HR, left ventricular developed pressure (LVDP) and ? dp/dtmaxd were recorded before ischemia and at 30 and 60 min of reperfusion. Coronary effluent was collected at 15 min of reperfusion for determination of CK and LDH activity. At the end of 60 min reperfusion the hearts were removed for determination of myocardial MDA content and SOD activity.Results LVDP and ? dp/dtmax recovered significantly better in 3-NPA group than in control group. The myocardial MDA content, CK and LDH release were significantly lower in 3-NPA group than in control group. The myocardial SOD activity was significantly higher in 3-NPA group than in control group. Conclusion Chemical preconditioning with 3-NPA protects the heart from I/R injury.

Objective To investigate the effectiveness of treating postherpetic neuralgia (PHN) by intradermal drug injection.Methods Ten rabbits of both sexes weighing 2.3-2.8 kg were anesthetized with intravenous urethane 1.5 g/kg. In group A (n = 5) 30% horseradish peroxidase(HRP) 500 ?l and in group B (n = 5) 1%-2% fluorescent nuclear yellow (NY) 500?l were injected intradermally at 6-8 points along the both sides of spine in the scapula region. After 48-72 h the animals were sacrificed and C4 -T10 spinal ganglia, cervical and thoracic sympathetic ganglia and celiac ganglia were harvested for identification of labeled neurocytes. Results Labeled neurocytes were found in C4-T10 spinal ganglia, cervical and thoracic sympathetic ganglia and celiac ganglia. There were more labeled neurocytes in the C6-T8 spinal ganglia. There were more labeled neurocytes in the sympathetic ganglia than in the spinal ganglia. The distribution of fluorescent labeled neurocytes corresponded to neurocytes labeled by HRP method. At the same segment there were more fluorescent labeled neurocytes than neurocytes labeled by HRP. Conclusion There is an ascending axoplasma streaming channel from nerver ending to the neurocytes in the ganglion as shown by morphological study and the good therapeutic effect of intradermal drug injection in the treatment of PHN may be related to this channel.

Objective: To observe the effect of on modified St.Thomas solution with mitochondrial ATP-sensitive potassium channel opener diazoxide on guinea pig papillary muscles protection after myocardial hypoxia. Methods: Twenty-four guinea pigs were randomly divided into three groups. In control group, cardioplegia was routine St.Thomas solution. In treatment group, cardioplegia was used modified St.Thomas solution. In blocker group, the muscle was treated with the specific potassium channel blocker glibenchamide 15 minutes before arrest used diazoxide cardioplegia. Myocardial electrophysical before and after cardioplegic arrest in guinea pig papillary muscles were studied. Results: 1, Time of recovery was shortened significantly in treatment group (P

Objective To supply the foundation of Shuyuwan as drug curing on wounded stress disease, by exploring its immunity pharmacology. Methods The effect of the Shuyuwan upon the wounded stress mice IL-2 mRNA and IL-2R mRNA gene expression were detected by Q-RT-PCR. Results The Shuyuwan used in the wounded stress mice can improve IL-2 mRNA and IL-2R mRNA level significantly (P

ObjectiveTo observe the curative effect of lumbar traction and Huantiao point-injection in the same side on lower limb pain induced by prolapse of lumbar intervertebral disc.Methods86 patients with lower limb pain induced by prolapse of lumbar intervertebral disc were randomly divided into the treatment group and control group with 43 cases in each group. Patients of the treatment group were treated by Huantiao point-injection in the same side and lumbar traction, and cases of the control group were treated by lumbar traction only.ResultsThe recovery rate of the treatment group was higher than that of the control group (P<0.01) after 3 weeks treatment.ConclusionThe effect of lumbar traction and Huantiao point-injection in the same side on lower limb pain induced by prolapse of lumbar intervertebral disc is better than lumbar traction only.

Objective To observe the effects of pulsed electromagnetic fields (PEMFs) on behavior and mechanical paw withdrawal thresholds (MPWTs) after acute skeletal muscle contusion (ASMC) in rats, and to in-vestigate the application of PEMFs in rats with ASMC during the early stage. Methods Forty-two Sprague-Dawley rats were randomly divided into a PEMF group (P group) , control group (C group) and blank control group ( BC group). ASMC models were set up in groups P and C, and no intervention was applied in the BC group. A PEMF was administered to animals in the P group immediately after the ASMC was inflicted. The behavior of the rats in each group was then observed. The MPWT of each rat was tested 2 days before and 0, 12, and 18 hours after the ASMC was inflicted). Results In the P and C groups, MPWT of the left hind paw at the 12th and 18th hour after ASMC was significantly lower than the baseline pain threshold 2 days before the ASMC. At 18 hours, the MPWT was signifi-cantly higher than at 12 hours in the P group. MPWT at 12 hours in the P group and at both 12 and 18 hours in the C group were significantly lower than in the BC group. MPWT in the P group at 18 hours was significantly higher than in the C group. Conclusions The behavior of rats treated with PEMF immediately after ASMC was improved, and their pain threshold was still elevated 18 hours later.

Objective To observe the effects of pulsed electromagnetic fields (PEMFs) on histological changes and myogenic differentiation factor D (MyoD) expression in rats with acute skeletal muscle contusion ( ASMC), and to explore the effects of PEMF therapy on rats with ASMC in its very early stages. Methods Forty-two rats were randomly divided into three groups : a treatment group, a control group and a blank control group. ASMC models were established with all the animals in the treatment and control groups. PEMF treatment was admin-istered to the treatment group immediately after the establishment of the ASMC model. Seven rats in each group were sacrificed at the 12th and 18th h after the models were set up. Their triceps surae muscles were sampled and treated with haematoxylin-eosin staining for study using immunofluorescence techniques and a fluorescence microscope. Re-suits In the control group at the 12th h and 18th h, HE staining showed pale cytoplasm and polymorphism in the cell nuclei ; in the treatment group these effects were significantly lighter, but in both groups it was more serious than in the blank control group. In the treatment and control groups, the fluorescence intensity of MyoD at the 18th h was higher than at the 12th h, and at each time point in both groups it was higher than in the blank control group. At the 18th h, fluorescence in the treatment group was stronger than in the control group. Conclusion MyoD expression in rats with ASMC is upregulated by thel8th h after early PEMF treatment. This might be one of the mechanisms ac-celerating the regeneration of skeletal muscles after trauma.