Objective To study the process of brain protein hydrolysate of inactivate and virus removal.Methods The Parvoviridae parvovirus genera of porcine parvovirus (PPV),vesicular stomatitis virus rhabdovirus genera of vesicular stomatitis virus (VSV) were chosen as a model virus,wherein PPV represents no envelope deoxyribonucleic acid(DNA) virus,VSV represents the envelope ribonucleic acid(RNA) virus.Simulation of the production process of virus inactivation steps 100 ℃ × 30 min,ultrafiltration as inactivation/removal condition.The virus respectively according to 1 ∶ 9 into the brain protein hydrolysate,high temperature and ultra filtration virus inactivation/removal.In pig kidney cells (PK-15) in PPV cell culture,Africa green monkey kidney cells(Vero cells) cultured VSV,determination of virus titer.Results PPV and VSV through the sterilization,virus median tissue culture infective dose(TCID50) were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs) ;removal processaverage virus reduction coefficient were 6.15log/0.1mL(logs),5.37 log/0.1mL(logs).Conclusion The high temperature and ultra filtration produces brain protein hydrolysate solution process are effective virus inactivation/removal process.

OBJECTIVEThis study aimed to evaluate the effort of applying frontal and occipital bones in extensive cranioplasty and preserving multiple cranial bone flaps adhered to the dura mater in the treatment of sagittal synostosis.

METHODSFrom April 2008 to June 2013, sixty-three children with sagittal synostosis, aged 5 months to 3 years, were included in the study. The frontal bone flap was removed using an air drill. The occipital and bilateral temporal bone flaps were cut open but not detached from the dura mater or fixed to produce floating bone flaps. The skull bone was cut into palisade-like structures. Brain compression from both sides and the base of the skull was released and the brain expanded bilaterally through the enlarged space. Only a long strip-shaped bone bridge remained in the central parietal bone. Subsequently, the frontal bone flaps and occipital bone flap were pushed towards the midline and fixed with the parietal bone bridge to shorten the anteroposterior diameter of the cranial cavity and allow the brain to expand bilaterally to correct scaphocephaly. The CT images showed that both sides of the parietal bone of artificial sagittal groove gradually merged postoperative 1 year, and skull almost completely normal healing after operation 2 or 3 years, without deformity recurrence within 5 years. Among them all, 61 children's intelligence is normal and 2 children's lagged behind normal level, no further improvement.

RESULTSPatients were followed up 1 - 5 years (an average of 43 months). Skull growth was excellent in all patients, the anteroposterior diameter was shortened by 14.6 mm averagely, the transverse diameter was increased by 12.3 mm averagely, the prominent forehead was corrected, and scaphocephaly improved significantly. There were no complications such as death and skull necrosis.

CONCLUSIONSThe application of frontal and occipital bones in extensive cranioplasty and preserving multiple cranial bone flaps adhered to the dura mater can be used in the treatment of sagittal synostosis. Surgery without removing bone flaps is less traumatic and results in no massive bleeding. It can effectively relieve brain compression and promotes transversal expansion of the brain during surgery and subsequent normal brain development.

Bone and Bones ; Brain ; growth & development ; Child, Preschool ; Craniosynostoses ; surgery ; Dura Mater ; Frontal Bone ; surgery ; Humans ; Infant ; Parietal Bone ; surgery ; Recurrence ; Surgical Flaps ; Temporal Bone ; surgery

Objective To investigate the protective effects of ShenFu injection on kidney of experimental acute pancreatitis in mrs. Methods Twenty and one Wister rats were randomized into the sham-operated group,AP group and Shenfu group. The plasma level of MDA,MPO,SOD,TNF-α and the kidney level of MDA、ON、SOD and historical scores was determined. Results The values of plasma SOD、MDA、MPO、TNF-α and the kidney of MDA、ON and historical scores in Shenfu-gronp were higher than that in the sham-operated group but lower than that in AP group. The values of SOD of plasma and kidney in Shenfu group is higher than that in AP but lower than that in the sharm-operated group. Coneluion Shenfu injection can protect the early injure of kidney of severe acute panereatitis,which mechanism may be to improve the kidney microcirculation,to reduce the production of TNF-α,to decrease the inflammation of plasma and kidney and to decrease the injure of oxygen free radicals.

Objective To explore the correlation of RELL1 expression with clinical pathological features and prognosis of patients with colon cancer. Methods Immunohistochemical experiments of the RELL1 protein were performed on tissue chips from 80 colon cancer tissues and 80 adjacent tissues. The relationship between different expression levels of RELL1 protein and clinical pathological parameters was compared. Univariate and multivariate Cox risk proportional regression analyses were conducted on factors affecting the survival of patients with colon cancer. Kaplan-Meier survival curve analysis was conducted on the survival rates of patients with colon cancer and different levels of RELL1 expression. Log rank test was performed to evaluate differences in survival rates. Results The expression of RELL1 in colon cancer tissues was lower than that in adjacent tissues (P<0.05). The expression of RELL1 in cancer tissues is correlated with TNM stage and N stage (P<0.05). The 3-year overall survival (OS) rate of colon cancer patients with high RELL1 expression was higher than that of patients with low RELL1 expression (P<0.05). Multivariate Cox regression analysis showed that low RELL1 expression, advanced age, and high TNM stage were risk factors for poor prognosis in patients with colon cancer (P<0.05). Conclusion The expression of RELL1 is downregulated in colon cancer tissue, and the low RELL1 expression, advanced age, and high TNM stage can lead to adverse outcomes in patients.