Objective To study chronic bronchitis rats oxidant/antioxidant related indicators Ho flavored Dingchuan Decoction.Methods 50 SD rats were adopted improved smoked method to make chronic bronchitis model.From the 20th day of the modeling,blank control group and model control group gavage were treated with saline.The positive control group were treated with Cough Mixture.The experimental groupwere dealed with Ho flavored Dingchuan Decoction high,medium and low-dose.20 day later,the cotent of SOD,CAT,MDA in rat serum,lung tissue and balf were observed.Results Compare to blank control group,the cotent of SOD[(188.11 ± 9.37)U/ml、(276.11 ±30.28) U/ml,(8.80 ± 1.49)U/ml],CAT[(9.40 ±2.04)U/ml,(26.57±4.26)U/ml,(1.58±0.26) U/ml] in rat serum,lung tissue of the experimental group significantly reduced (P ＜0.01) ; MDA [(5.64 ± 0.52) μmol/L,(6.32 ± 2.10) μmol/L,(28.08 ± 2.43) μ mol/L) increased (P ＜0.05 or P ＜ 0.01).Ho flavored Dingchuan Decoction can effectively reverse these changes,and showed a significant dose-dependent.The Ho flavored Dingchuantang,in middle and high-dose serum SOD [(253.05 ± 7.81) U/ml,(256.92 ± 5.37)U/ml],CAT [(14.86 ± 2.49)U/ml,(18.22 ± 2.65)U/ml] higher than those of blank control group.And the Ho flavored Dingchuantang,in middle and high-dose lung tissue SOD [(470.91 ± 11.46)U/mgprot,(482.91 ± 14.32)U/mgprot),CAT [(46.87 ± 3.75)U/mgprot,(55.49 ±3.33)U/mgprot] higher than those of blank control group.Content close to the normal level.Conclusion The Ho flavored Dingchuan Decoction has significantly reduce the oxidative stress in chronic bronchitis.

The activity of eNOS was singificantly depressed by a high concentration of glucose in a concentration-and time-dependent manners after incubation of HUVECs with different concentrations of glucose and with high glucose plus insulin for different times.The physiological concentration of insulin can partially reverse the inhibitions of the activity and expression of eNOS induced by high concentration of glucose.

ObjectiveTo observe the expression of mCD14 and human leucocyte antigen (HLA)-DR on peripheral blood mononuclear cell( PBMC ) and cytokine in severe sepsis and its significance.MethodsThirty-five patients with severe sepsis (patients group) and 15 healthy volunteers (control group)were selected in this study.The expression of mCD14,HLA-DR on PBMC,serum tumor necrosis factor (TNF)- α,interleukin(IL)-10,the total score of acute physiology and chronic health evaluation(APACHE Ⅱ )and sepsis-related organ failure assessment (SOFA) score of the patients were measured at the 1st,3rd,5th day after admission.ResultsThe expression of mCD14 and HLA-DR on PBMC,the levels of serum TNF- α and IL-10 were ( 2.61 ± 1.59 )％,( 10.25 ± 5.35 )％,(96.66 ± 45.38) ng/L,( 149.74 ± 77.15 ) ng/L in patients group,(5.57 ± 1.53)％,(59.28 ± 14.76)％,(0.12 ±0.00) ng/L,(5.67 ±2.16) ng/L in control group,there were significant differences between two groups (P ＜ 0.05 or ＜ 0.01 ).In patients group,the mortality of 28 days was 28.6％(10/35).The expression of mCD14 and HLA-DR on PBMC,SOFA score and APACHE Ⅱ at the 1st,3rd day showed no statistical significance between non-survivor patients and survivor patients (P ＞ 0.05 ),but at 5th day the expressions of mCD14 and HLA-DR on PBMC in survivor patients were significantly higher than those in non-survivor patients [ (5.12 ± 2.03 )％ vs.(2.75 ± 0.67 )％; (35.12 ±9.29)％ vs.(13.06 ±5.87) ％](P＜0.01 or ＜ 0.05),SOFA score and APACHE Ⅱ were significantly lower than those in non-survivor patients[ (4.48 ± 1.71 ) scores vs.( 10.70 ± 3.16 ) scores; (9.36 ± 5.57 ) scores vs.(25.60 ± 10.88) scores](P＜ 0.01 ).The levels of serum TNF- α and IL-10 at the 1st,3rd,5th day showed no statistical significance between non-survivor patients and survivor patients(P ＞ 0.05).Conclusions The expressions of mCD14 and HLA-DR on PBMC in severe sepsis show closely related to the outcome.The changes of serum TNF- α and IL- 10 can not reflect the prognosis of severe sepsis in 5 days.

Objective To study the reporting status of notifiable infectious diseases in a general hospital,grasp epidemiological characteristics and patterns,and provide scientific basis for the preventive measures of infectious diseases.Methods Epidemic data of notifiable infectious diseases reported by a hospital in 2007 -2011 were ana-lyzed statistically.Results Reporting rate of notifiable infectious diseases was 0.40%(15 386/3 852 327)in 2007-2011 ,and were statistically different among each year (χ2 =94.93,P <0.01),the major age of reported cases were 14-45 years(46.70%),the main transmission routes were blood and sex (60.68%),followed by respiratory tract (33.06%).The reporting rates among different months were statistically different(χ2 =487.60,P <0.01 ),April ranked first for five consecutive years(0.53%),followed by December(0.51 %)and May(0.48%).The main repor-ted infectious diseases included viral hepatitis(42.82%),varicella(20.06%),syphilis(16.96%),rubella(5.41 %), and pulmonary tuberculosis(3.75%).Conclusion Bloodborne diseases,sexually transmitted diseases,and infec-tious respiratory diseases are the stress in the prevention and control of infectious disease,management should be in-tensified.

Objective To investigate the cardioprotective effects of 3-nitropropionic acid (3-NPA) pretreatment against ischemia-reperfusion (I/R) injury. Methods Sixteen male Wistar rats weighing 200-250 g were randomly divided into 2 groups ( n = 8 each):(1) 3-NPA group received intraperitoneal 3-NPA 4 mg?kg-1 24 h before the animals were sacrificed and (2) control group received normal saline instead of 3-NPA. The animals were sacrificed and the hearts were immediately removed and mounted on Langendorff apparatus and perfused with K-H solution saturated with 95% O2 and 5% CO2 at 37℃ . After being perfused for 30 min the hearts were subjected to 30 min global ischemia by suspension of perfusion followed by 60 min reperfusion. The HR, left ventricular developed pressure (LVDP) and ? dp/dtmaxd were recorded before ischemia and at 30 and 60 min of reperfusion. Coronary effluent was collected at 15 min of reperfusion for determination of CK and LDH activity. At the end of 60 min reperfusion the hearts were removed for determination of myocardial MDA content and SOD activity.Results LVDP and ? dp/dtmax recovered significantly better in 3-NPA group than in control group. The myocardial MDA content, CK and LDH release were significantly lower in 3-NPA group than in control group. The myocardial SOD activity was significantly higher in 3-NPA group than in control group. Conclusion Chemical preconditioning with 3-NPA protects the heart from I/R injury.

Objective To investigate the effects of comprehensive intervention on population death rate. Methods To analyze population crude death rate, cause-specific death rate, and compare the death rate in intervention group with that of control.Results The average crude death rate in communities of Changsha was 617 79 per 100 000, with 557 27 per 100 000 of standarized death rate. Cerebrovascular disease, cardiocascular disease, tumour, disease of respiratory system injury and intoxication were from the first rank to the fifth in order the death causes. The death rate in intervention group was lower than that of control group significantly, and the death rate of cerebrovascular disease and cardiocascular disease in intervention group were lower than that of control significantly.Conclusions Cerebrovascular disease and cardiocascular disease are the major causes of death. Intervention on risks factors of cardio- and cerebrovascular diseases can lower the death rate of cardio- and cerebrovascular diseases and the population death rate.

Objective: To observe the effect of on modified St.Thomas solution with mitochondrial ATP-sensitive potassium channel opener diazoxide on guinea pig papillary muscles protection after myocardial hypoxia. Methods: Twenty-four guinea pigs were randomly divided into three groups. In control group, cardioplegia was routine St.Thomas solution. In treatment group, cardioplegia was used modified St.Thomas solution. In blocker group, the muscle was treated with the specific potassium channel blocker glibenchamide 15 minutes before arrest used diazoxide cardioplegia. Myocardial electrophysical before and after cardioplegic arrest in guinea pig papillary muscles were studied. Results: 1, Time of recovery was shortened significantly in treatment group (P

AIM: To comparatively analyze the pain related factors levels and therapeutic response in patients treated with ~(99)Tc-MDP and ~(153)Sm-EDTMP for painful skeletal metastases. METHODS: Plasma endothelin (ET), calcitonin gene-related peptide (CGRP), thromboxane B_2 (TXB_2) and 6-keto-prostaglandin F_ 1? (6-k-PGF_ 1? ) levels were analyzed in 93 patients with painful skeletal metastases prior and 3 months after treatment. 55 cases were just treated with 153 Sm-EDTMP (group A); 19 cases were treated only with 99 Tc-MDP (group B); and 19 cases were treated with both 153 Sm-EDTMP and 99 Tc -MDP (group C). RESULTS: 69.1 %, 73.7 % and 89.5 % of the patients were experienced pain relief 3 months after treatment in groups A, B and C, respectively. Comparative analysis shows that: ET and 6-k-PGF_ 1? levels increased significantly 3 months after treatment in all patients (P

Objective To explore the relationship between paraoxonase1 (PON1) L55M gene polymorphisms and atherosclerotic cerebral infarction(CI)in Hunan Han people. Methods The study population was comprised of 153 atherosclerotic cerebral infarction patients and 153 healthy individuals. The PON1-L55M genotypes were determined by restriction fragment length polymorphism. Results MM genotype was not found both in CI and control group. In CI group, genotypic frequency of LL was 96.7% and LM was 3.3%. The allele frequency of L was 0.984 and M was 0.016. In control group, genotypic frequency of LL was 93.5% and LM was 6.5%. The allele frequency of L was 0.968 and M was 0.032. PON1-L55M polymorphism revealed no significant difference of genotype and allelic distribution in CI patients and controls.Conclusion The polymorphism of PON1-L55M is not related to the incidence of atherosclerotic cerebral infarction in Hunan Han people.

0.05),while significant differences were observed among different involvement depth,size,and location of tumors(P