Objective To study the influence of aging and chemotherapeutic drugs of breast cancer patients on chemotherapy-induced amenorrhea (CIA) and to guide the further selection of drugs for chemotherapy.Methods We prospectively studied the CIA of the premenopausal breast cancer patients and compared the difference in CIA between the patients who were at different aging periods or received different chemotherapeutics. Results 137 patients were followed up.The occurrence rate of CIA and long-term chemotherapy-induced amenorrhea(LCIA)were 73.72%(101/137)and 43.80%(60/137)respectively.The rate of CIA and LCIA was significandy higher in older women(＞40 years)than young women(≤40 years,X2=25.32,18.42,P＜0.05).The recovery rate of CIA in young women was 61.90%(13/21)which was significantly higher than the older women[35.00%(28/80);X2=4.99,P=0.025].The difference in the rate of LCIA was statistically significant between the older women (＞40 years)who received different anthracyclinebased chemotherapy(X2=6.92,P=0.031).Conclusion Age is an important factor for the CIA.Chemotherapy has less influence on the menstruation of younger women(≤40) and most amenorrhea is reversible.For older women,the rate of LCIA is different in patients who receive imported epirubicin,domestic epirubicin or pirarubicin.

Destroyed lung has resulted in the pathological changes of irreversible lung damage. The variety of reasons cause lobes of lung or the side of the whole lung loss of function and volume decrease. To the side of lung damage for patients.,it is necessary to take the whole side of surgical resection of lung disease at the right time Not only it can quickly alleviate the respiratory symptoms, but also can significantly improve the patients quality of life. Even some benign disease can be cured to achieve the purpose. Because of the extensive fibrosis of lung tissue loss of the affected lung function. Patients have varying degrees of shortness of breath and recurrent cough, cough purulent sputum, hemoptysis. Because of the thickened parietal pleura,those result in the effect of bad medical treatment. Most need surgical treatment, but the surgery cause perioperative patient physiological condition of interference, Major trauma surgical procedures, much more bleeding, and many and serious postoperative complications, those require a higher surgical technique. The article summed up the whole lung damage lung surgical methods and progress were reviewed.

The majority of women diagnosed with early-stage breast-cancer have an excellent long-term prognosis, but many still suffer from worsened life quality and infertility caused by chemotherapy-induced amenorrhea(CIA). The risk of CIA varies by patient age, but is not significantly influenced by chemotherapeutic regimens. CIA will improve disease-free survival and overall survival for patients with hormonal receptor positive tumors. For low-risk patients and patients with the intention of maintaining fertility, proper treatments need to be chosen to preserve their reproductive function.

Objective To define the correlation between nasopharyngeal carcinoma (NPC) cell radiosensitivity and gene mutation in the ATM/PI3K coding region. Methods The gene mutation in the ATM/PI3K region of nasopharyngeal carcinoma cell lines which vary in radiosensitivity,was monitored by reverse transcription polymerase chain reaction (RT PCR) and fluorescence marked ddNTP cycle sequencing technique.Results No gene mutation was detected in the ATM/PI3K region of either CNE1 or CNE2.Conclusion Disparity in intrinsic radiosensitivity between different NPC cell lines depends on some other factors and mechanism without being related to ATM/PI3K mutations.

Objective To compare the effect of spinal anesthesia by using 0.75% ropivacaine or 0.5% bupivacaine in a simulated state of rocking naval vessels, in order to choose an optimal anesthetic agent in a rocking naval vessels at sea. Methods Eighty patients undergoing elective surgery of abdomen or lower limb were randomly divided into 4 groups (n=20). Namely, horizontal level with 0.75% ropivacaine control group (R1), horizontal level with 0.5% bupivacaine control group (B1), simulated rocking with 0.75% ropivacaine group (R2), simulated rocking with 0.5% bupivacaine group (B2). The effect of anesthesia, the changes in hemodynamics and the incidence of relevant side-effects were determined and compared among the four groups. Results The anesthetic level (analgesic level) of group B2 was 2.7 segments higher than that in group B1, and the durations for fixed level was longer than group B1 (P

500?mol/L) of propofol was needed; In biological system near the range of anaesthetic concentration, propofol effectively scavenged oxygen free radical produced by respiratory burst of PMN. Conclusion: Propofol scavenging hydroxyl radical generated in biological and nonbiological system,may be one of the main mechanisms of its antioxidation,the intralipid component of Diprivan may affect its antioxidation.

The result of medical licensing examination is an objective reflection of the quality of medical education. The average scores and pass rates of medical licensing examination of the military medical university including The Third Military Medical University, were under the national average levels, which made us find the problems, therefore, reflection on curriculum structure, teaching method, administration mode and continuing education should be integrated to the requirement of the medical licensing examination, so as to let the students meet the complex demand of the society, as well as the special requirement of military service.

Objective To investigate the effect of exercise preconditioning on serum level of tumor necrosis factor α (TNF-α), interleukin (IL) -1β and IL-6 in rats after cerebral ischemia-reperfusion (I/R). Methods 24 male Sprague-Dawley rats were randomly divided into exercise preconditioning group (n=8), model group (n=8) and sham group (n=8). The middle cerebral arteries were occluded for 120 min and re-perfused. All the rats were evaluated with neurological deficit score 2 hours, 24 hours after I/R. The serum levels of TNF-α, IL-1β and IL-6 was detected with enzyme-linked immunosorbent, and the pathology was observed with HE staining 24 hours after I/R. Results The neurological deficit score decreased in the exercise preconditioning group compared with that in the model group, as well as the serum TNF-α, IL-1β and IL-6 (P<0.05) 24 hours after I/R. The pathological damage and interstitial edema alleviated in cerebral ischemia cortical, and the degeneration and necrosis of the neurons in the ischemic area significantly reduced in the exercise preconditioning group. Conclusion Exercise preconditioning may inhibit inflammatory response in I/R rats to protect neurological function from impairment.

Objective To induce adipose-derived mesenchymal stem cells (ADMSCs) differentiation into cardiomyocytes, so as to provide stem cells for myocardial regeneration.Method ADMSCs were isolated and cultured from adult adipose tissue in vitro.They were induced with 10μmol/L 5-azacytidine (5Aza) for 24h.At the 7th, 14th, 21st days after induction, the expression of α-sarcomeric actin, and myosin heavy chain were repeatedly detected by immunocytochemistry.The expression of ANP was detected by RT-PCR.Results At the 7th day after induction, there was no expression of α-sarcomeric actin and MHC.At the 14th day, there was a little expression of α-sarcomeric actin and MHC that could be seen in cells.At the 21st day, there was increased expression of α-sarcomeric and MHC, and the expression of ANP was positive results detected by RT-PCR.Conclusions 5-Aza can induced ADMSCs to differentiate into cardiomyocytes.ADMSCs might be a potential source of cell transplantation for myocardial.

In this paper,the constitute elements of Sino-US licensed doctors' practical skill examination evaluation index system are expounded.Through comparison,the differences on the formulation basis,classification principle,test content,and ability requirements are inferred;On the basis of referring to the advanced experience and practice of the United States,and by combining the requirements of the most basic requirements of global medical education,suggestions for improving the Chinese licensed doctors' practical skill examination evaluation index system are proposed in terms of integrating the test syllabus classification,adjusting the distribution of clinical practice ability examination content,increasing the proportion of medical humanities content test,as well as establishing a scientific examination evaluation index weight table.