This study was aimed to provide experimental evidence for clinical application of curcumin to strengthen the effect of 5-Fluorouracil (5-FU) in gastric cancer treatment, so as to reduce the dose of 5-FU and its side-effect in the future treatment. MTT assay was used to detect medication effect on MGC-803 cell growth inhibition; AO/EB double staining fluorescence microscopic observation and FITC/PI double staining flow cytometry were used to detect medication effect on cell apoptosis. Flow cytometry was based on PI staining used to detect medication effect on MGC-803 cell cycle. The results showed that curcumin (25μmol·L-1) with low-dose (2.4μmol·L-1) and middle-dose (4.8μmol·L-1) 5-FU can effectively inhibit MGC-803 cell growth, induce cell apoptosis and block cell cycle in S phase. Which showed dose-time dependent manner. The combined use of curcumin with low-dose 5-FU was more effective than the middle-dose (4.8μmol·L-1) 5-FU alone (P < 0.01); similarly, the combined use of curcumin with middle-dose 5-FU was more effective than the use of high-dose (9.6μmol·L-1) 5-FU alone (P < 0.01). It indicated that curcumin can enhance the antitumor effect of 5-FU against MGC-803 cells in a dose-time dependent manner. It was concluded that the study provided a preliminary experimental basis for using curcumin as an adjuvant to 5-FU with the benefit of reducing its dose and toxicity in the clinical treatment of gastric cancer.

Objective To explore the effects of behavioral activation (BA) intervention on patients with chronic obstructive pulmonary disease (COPD) complicated with anxiety and depression. Methods Totally 70 patients with COPD complicated with anxiety and depression who were hospitalized in a ClassⅢ Grade A hospital from August 2018 to April 2019 were selected using convenient sampling and divided into experimental (n=35) and control (n=35) groups according to the time of admission. Patients in the control group received conventional hospital care and psychological guidance in the Department of Respiratory Medicine, while patients in the experimental group received BA intervention on this basis. Anxiety, depression and fatigue scores were compared between the two groups. Results A total of 33 patients in control group and 32 patient in experimental group completed the study. After BA intervention, the anxiety and depression scores of the experimental group were lower than those of the control group, and there were statistically significant differences between the two groups (P＜0.05). The total score of multi-dimensional fatigue scale of COPD patients in the experimental group was lower than that of the control group, the difference was statistically (P＜ 0.05), and there was no statistically significant difference in the score of mental fatigue between the two groups (P＞0.05). Conclusions BA intervention can ameliorate the anxiety and depression in patients and reduce their sense of fatigue, thus improving their quality of life.