1.Analysing the value of postprocessing technique with multi-slice helical CT in detection of the occult facture
Jie LI ; Yunchao ZHAO ; Zhenxian MA ; Yu DONG
The Journal of Practical Medicine 2016;32(15):2474-2476
Objective To investigate the value of postprocessing technique of multi-slice helical CT for the occult fracture located in complicated structure. Methods One hundred-thirteen patients with acute trauma but negative on plain X-ray film were reviewed retrospectively. All of the patients underwent MSCT ,and original data were reformed with reconstruction. Results Of one hundred-thirteen injuried patients , fractures were demonstrated with MSCT imaging in forty-eight cases. Conclusion The combination use of axial image of MSCT , reconstruction and post-processing is of high value in definite detection of occult fracture , and play an important role in selecting therapy project and evaluating prognosis.
2.The research on inflammatory reaction and differential expression of microRNAs in lung caner
Dongxu JIN ; Guangqiang ZHAO ; Yunchao HUANG ; Hu LI
Journal of International Oncology 2014;(7):518-520
MicroRNAisatypeofsingle-strandednon-codingRNA,directlybondingtothecomplemen-tary target gene mRNA.That leads to the inhibition of mRNA translation of the target molecule in order to reduce expression of the target gene.MicroRNAs are not only involved in the regulation of inflammatory reac-tion,but also play important roles in the formation of neoplasm.The researches showing that the inflammatory reaction interacting with miRNAs results in the differential expressions of microRNAs in lung cancer have been widespreadly concerned.Along with the deepening of the research on the mechanisms of inflammatory reaction and differential expression of microRNAs,it will provide new strategies for the prevention,diagnosis and treat-ment of lung cancer.
3.Influence of plasma protein on bacterial adhesion to biomaterials
Yanxing LI ; Suhua XIONG ; Yuming ZHAO ; Yunchao HUANG
Chinese Journal of Tissue Engineering Research 2008;12(36):7195-7198
Implant-associated infection is a serious problem in the clinical application of biomaterials.which greatly restricts the generalizatjon of cardiovascular biomaterials,while bacterial adhesion is the initial cause for implant-associated infections of cardiovascular biomaterials.Therefore.the prevention of bacteriaI adhesion to the biomaterials plays a crucial role in the cure of biomaterial center infection.It has reported that human plasma protein influences the bacterial adhesion to biomateriais.Some plasma proteins inhibit bacterial adhesion.whereas some promote jt.Study on the correlation between plasma protein and hacterial adhesion to the biomaterials is of great importance in the prevention and cure of biomaterials center infection.
4.Construction of a qseC-deleted mutant of Escherichia coli and evaluation of biofilm formation of the mutant
Kun YANG ; Yujie LEI ; Yunchao HUANG ; Lianhua YE ; Guangqiang ZHAO
Chinese Journal of Microbiology and Immunology 2011;31(9):776-780
Objective To construct a qseC-deleted mutant strain of E.coli by Red recombination and to study the effect of qseC gene on biofilm formation in the mutants.Methods The chloramphenicolresistant gene flanked by homologues of target genes was amplified by PCR and electro-transformed into E.coli MC1000.When induced by L-arabinose,the plasmid pKD46 could express three recombinant proteins of λ-prophage,which led to the replacement of target gene(qseC) with chloramphenicol-resistant gene.Then the chloramphenicol-resistant gene was eliminated by FLP-promoted recombination events.The biofilm formation of wild-type and mutant strain was detected by crystal violet staining.Results The qseC-deleted mutant of E.coli was confirmed by various PCR and DNA sequencing.Gene qseC was completely deleted.There was no significant difference in growth ability between the qseC mutant strain and the wild-type strain MC1000.The biofilm formation of wild-type and mutant strain was quantified by crystal violet staining.The absorbance determined with a plate reader at 570 nm was 1.00±0.15 and 0.47±0.10 respectively.Conclusion The qseC-deleted mutant of E.coli was constructed successfully.And the qseC gene plays an important role in regulation of biofilm formation in E.coli.
5.Application of multi-slice spiral CT angiography in diagnosis of cerebral vascular diseases
Dong ZHAO ; Yunchao WANG ; Lina JIANG ; Kejiang WANG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(11):1778-1779
Objective To evaluate the application of multi-slice CT angiography(MSCTA) in cerebral vascular diseases.Methods 16-slice spiral CT angiography of 32 patients which clinically suspected cerebrovascular diseases were analyzed retrospectively.Source images were processed by GE Lightspeed CT Scanner;all axial images were transferred to an external workstation.The reconstructed images were processed VR,MPR,MIP in working-station.Results 10 of the 32 patients were normal, 17 were aneurysms,could be clearly displayed with the source, size,and neck width.3 were AVM,the location and scope could be observed.2 were vaso-occlusive.Conclusion Multislice spiral CT angiography is a safe ,quick and noninvasive method in diagnosing the cerebrovascular disease,it may be proposed as a first choice technique for screening and preoperative assessment for cerebrovascular disease.CTA is a valuable diagnostic method for various intracranial cerebral disease.
6.Application of Intrapericardial Pneumonectomy and Atria Part Resection in Lung Cancer Operation
Kaiyun YANG ; Yunchao HUANG ; Yujie LEI ; Guangqiang ZHAO ; Xu LI ; Xi WANG
Journal of Kunming Medical University 1989;0(01):-
Objective To explore the significance and curative effects of intrapericardial pneumonectomy and atria part resection in lung cancer operation.Methods Fifty eight patients of lung cancer were performed with intrapericardial pneumonectomy and atria part resection.Results The operative mortality rate was 0.The incidence rate of complications in post-operation was 36.21%,a majority of which was arrhythmia and occupied 18.97%.Conclusion The application of intrapericardial pneumonectomy and atria part resection in lung cancer operation can not only improve the resection rate but also can offer the opportunity of absolute resection and further therapy in a part of lung cancer patients.
7.Analysis of the Clinical Characteristics of Malignant Chest Tumor Patients with Nosocomial Mixed Fungal-Bacterial Mixed Infection
Wanshi DUAN ; Ying CHEN ; Yunchao HUANG ; Guangqiang ZHAO ; Qiubo HUANG ; Yan LU
Journal of Kunming Medical University 2016;37(8):60-64
Objective To explore the clinical characteristics of and to provide help to the prevention and treatment of malignant chest tumor with nosocomial mixed fungal-bacterial infection.Methods From July 2007 to June 2015,pathogenic bacteria in sputum,blood,urine,chest incision,thoracic and abdominal fluid,and implantable medical biological material were cultivated in 5067 patients with malignant chest tumor suspected with infection.The clinical characteristics,source of specimen and pathogenic bacteria,the types of diseases,medical intervention activities of 142 cases detected with mixed fungal-bacterial infection were retrospectively analyzed.Fesult In 142 patients,104 patients at clinical stage Ⅲ-Ⅳ accounted for 73.2%,and 94 patients used antibiotics more than 14 days (66.2%);104 cases had implanted biological materials (74.7%);96 cases died (67.6%).A total of 167 strains bacteria were isolated.Sixty-one strains of G+ bacteria accounting for 36.5% were mainly Epidermis staphylococcus and Staphylococcus aureus;106 strains of G-bacteria accounting for 63.5% were mainly klebsiella pneumonia,Escherichia coli and baumanii;172 strains fungus mainly of Candida albicans were isolated (77.3%).Pathogenic bacteria sources were mainly sputum specimens + pharynx strip,blood culture and medical implant materials.In 72 lung cancer patients,squamous carcinoma and small cell carcinoma were 52.8% and 33.3% respectively,higher than adenocarcinoma (12.5%);In 42 esophageal cancer patients,postoperative patients were 42.9%.Parenteral nutrition patients with more than 10 days were 80.9% higher than that of parenteral nutrition in patients with less than 10 days (19.1%).Conclusion Among malignant chest tumor patients with nosocomial mixed fungal-bacterial infection,the bacteria were found in staphylococcus aureus,klebsiella pneumoniae and E.coli and the fungus was Candida albicans.For clinical stage Ⅲ-Ⅳ,patients with parenteral nutrition for more than 10 days,having history of chemo or radiotherapy,with antimicrobial use for more than 14 days,and with implanted biological materials,should be warned about nosocomial mixed fungal-bacterial infection.
8.Effects of prone ventilation on extra pulmonary acute lung injury
Maoxian YANG ; Yunchao SHI ; Jing SHU ; Huijie YU ; Peng SHEN ; Pengcheng XU ; Wenjing ZHAO
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care 2014;(4):294-297
Objective To investigate the effects and mechanism of prone ventilation in rats with lipopolysaccharide(LPS)-induced extra pulmonary acute lung injury(ALI). Methods Thirty-two male Sprague-Dawley(SD)rats were randomly divided into four groups:control group,ALI group,supine position group(ALIS group)and prone position group(ALIP group). The ALI animal model was reproduced by intra-peritoneal injection of LPS 6 mg/kg,and 2 mL/kg sterile saline was intra-peritoneally injected in control group. After 24 hours,the ALIS group and ALIP group were mechanically ventilated for 4 hours in supine and prone positions respectively. Arterial partial pressure of oxygen(PaO2),carbon dioxide partial pressure(PaCO2),wet to dry weight ratio of lung(W/D) were observed,and the levels of tumor necrosis factor-α(TNF-α),interleukins(IL-6,IL-10)in bronchoalveolar lavage fluid(BALF)were measured. The degrees of histopathological changes of lung were investigated and assessed under light microscope. Results Compared with control group,PaO2 was significantly lower in ALI,ALIS and ALIP groups〔mmHg(1 mmHg=0.133 kPa):68±7,82±8,93±7 vs. 116±7〕,and the PaCO2 in ALI and ALIS groups was obviously higher(mmHg:53±6,51±5 vs. 43±7,both P<0.05);lung W/D ratio as well as levels of TNF-α, IL-6 and IL-10 in BALF were significantly higher in ALI,ALIS and ALIP groups〔lung W/D ratio:5.11±0.32, 4.71±0.38,4.53±0.45 vs. 4.48±0.15,TNF-α(ng/L):548±82,431±64,347±53 vs. 162±16,IL-6(ng/L):772±119,587±178,490±139 vs. 127±16,IL-10(ng/L):211±43,295±36,352±59 vs. 104±31,all P<0.05〕. Compared with ALI group,PaO2 in ALIS and ALIP groups was significantly higher,and in ALIP group,PaCO2 was lower,lung W/D ratio,TNF-α and IL-6 in BALF were obviously lower,while IL-10 in BALF was obviously higher(all P<0.05). Compared with ALIS group,the changes in ALIP group were more significant(all P<0.05). Compared with ALIS group,under light microscope,it was shown that the hyper-inflated areas were less,normal and collapsed areas were greater on the ventral side,and the collapsed area was less and the normal ventilation area was greater on the dorsal side in ALIP group(all P<0.05). Conclusion In rats with LPS-induced extra pulmonary ALI,the prone ventilation can improve gas exchange,promote uniform distribution of ventilation,decrease pulmonary edema and in the mean time attenuate inflammatory response.
9.Relationship between bacterial adhesion to prosthetic valve materials and bacterial growth
Yunchao HUANG ; Kaiyun YANG ; Yujie LEI ; Guangqiang ZHAO ; Xiaoming LI ; Jia GAO
Chinese Journal of Tissue Engineering Research 2008;12(39):7777-7780
BACKGROUND:Studies have demonstrated that prosthetic valve endocarditis is primarily caused by bacteria adhering on the surface of the materials.Thus,the relationship of prosthetic valve materials with bacteria adhesion and growth is an important subject.OBJECTIVE:To explore the influence of prosthetic valve materials on bacteria growth through observing the relationship of bacteria adhesion on prosthetic valve materials and bacteria growth.DESIGN,TIME AND SETTING:Repetitive measurement was performed at the Department of Cardiac and Thoracic Surgery,Third Hospital of Kunming Medical College from January to March 2001.MATERIALS:Terylene(Dacron)was purchased from Man-made Blood Vessel Laboratory of Suzhou Weaves Belt Factory;polytetrafluoroethylene was provided by Teflon-GoreTexW.L.Gore & Associates,Inc.Arizona,USA;pyrolytic carbon was provided by Department of Biological Material of Sichuan Union University;staphylococcus anreus,Escherichia coli,staphylococcus epidermidis,and Pseudomonas aerugmosa were prepared by our laboratory.METHODS:The growth curve of staphylococcus aureus,staphylococcus epidermidis,Escherichia coli and Pseudomonas aeruginosa on dacron,pyrolytic carbon and pelytetrafluoroethylene were quantitatively determined respectively by plate counting and gamma.ray counting of 125 Ⅰ radiolabeled bacteria in vitro.Bacteria growing normally served as control.All bacteria were cultured for 30 hours,and bacteria concentration was determined every 2 hours.In addition,the adhesive capacities of foMr kinds of bacteria on dacron,pyrolytic carbon and polytetrafluoroethylene were detected Escherichia coli and Pseudomonas aeruginosa on dacron,pyrolytic carbon and polytetrafluoroethytene.RESULTS:There were no significant differences in adhesive capacities of each bacterium on dacron,pyrolytic carbon and polytetrafluoroethylene at the same time point(P>0.05).The differences in growth curve of four kinds of bacteria on prosthetic valve materials were not remarkable compared to the control(P>0.05).Different bacteria showed different adhesion degree on the materials:staphylococcus aureus exhibited strongest adhesion on dacron;staphylococcus epidermidis on pyrolytic carbon;Escherichia coli on dacron.The adhesive capacity of Pseudomonas aerugmosa on dacron reached peak within 12 hours,and gradually decreased,but maintained strong adhesion on the other materials.The adhesive capacmes of four bacteria on the materials did not increase or maintain with time.CONCLUSION:The adhesive capacity of one bacterium to different artificial valve materials and different bacteria to one prosthetic valve materials is different.The materials.show little influence on bacterium growth cycle.
10.Research on the clinical application value of bone turnover markers in bone metastases from non-small cell lung cancer
Zhiyu WANG ; Chen YANG ; Yunchao GAO ; Zan SHEN ; Yumei YANG ; Hui ZHAO ; Yang YAO
China Oncology 2010;20(3):202-206
Background and purpose:Bone metastases leads to the destruction of bones by changing the level of bone turnover markers.The purpose of this study was to assess the clinical application value of bone turnover markers in bone metastases for non-small cell lung cancer,which included the diagnosis and spread behavior of bone metastases.Methods:AKP,β-CTx,OST and BALP were measured in 76 NSCLC with bone metastases patients and 44 normal people.Results:The level ofAKP,β-CTx and BALP in patients with bone metastasis was significantly higher than in the subjects without bone metastases.There were significant correlations among the bone turnover markers.The levels of BALP and OST were significantly positively correlated with the extent of bone metastasis.Patients with high-levels of CTx and low-levels of BALP had a higher risk of pathologic fracture.Conclusion:In patients with bone metastases from NSCLC,bone turnover markers can help make diagnoses and evaluate severity of disease.It potentially has a wide range of uses in clinical practice.