1.High Expression, Purification and Immuno-indentification of Recombinant HIV Surface Antigen gp120
Chen, CHEN ; Kun, LIU ; Jin-zhong, WANG ; Ming, MA ; Yun-qi, CENG ; Qi-min, CHEN ; Xiao-ke, ZHU ; Jian-hui, XIA
Virologica Sinica 2001;16(2):109-113
Different-length fragments of the HIV trans-membrane antigen (gp120) gene were expressed in Escherichia coli, using T7 expression system. SDS-PAGE stained by Coomassic Brilliant Blue showed no expression of full-length gp120 and poor expression of half-length gp120 fragments from the N-terminal, but high expression of 1/3-lgenth gp120 gene fragments from the N-terminal (including V1/V2 epitopes), more than 18% of total bacterial protein. Western blot showed fairly good reactivity to serum from HIV-infected individual. On this basis, we expressed the corresponding tp 120 fragments of the HIV strains epidemic in China. This study laid a solid foundation for exploration of high expression of gp 120 in E.coli and development of HIV serological diagnostic system for Chinese people.
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