Objective To discuss the relationship between the incidence of lymph node metastasis and tumor size in patients with non small cell lung cancer,and to provide evidence for clinical operation and follow -up treat-ment.Methods 180 patients with non small cell lung cancer who were hospitalized and recieived Lobectomy opera-tion were selected,the clinical data of patients and resection of tumor size,lymph node metastasis were retrospectively analyzed,and then the relationship between the lymph node metastasis and tumor volume was investigated.Results 180 cases of patients with lymph node metastasis rate was 37.20%,in which hilar lymph node metastasis rate was 11.02 percent and mediastinal lymph node metastasis rate was 10.95%.Lung lymph node metastasis rates in lymph node metastasis,hilar and mediastinal lymph node metastasis of T1a were 13.21%,15.09% and 13.21%;those of T1b period were 38.09%,35.71% and 28.57%;of T2a period were 44.44%,37.78% and 31.11%;of T2b period were 64.26%,57.14% and 53.57%;and those of T3 period were 58.33%,66.67% and41.67%.Lymph node metastasis,hilar and mediastinal lymph node metastasis in lung lymph nodes transfer rate with patients of T1a had significantly differences compared with patients of T1b,T2a,T2b and T3 stage.(χ2 =5.363,6.175,4.372,8.778, 5.363,9.631,7.936,9.631,20.463,7.521,6.175,4.387,all P <0.05).Hilar lymph node metastasis and lung metastases of T1b and T2b had a significant difference (χ2 =12.353,4.585,all P <0.05).Conclusion For non -small cell lung cancer,tumor size is positively correlated with lymph node metastasis and the greater the tumor size, the greater the likelihood of lymph node metastasis.

Immunoglobulin A nephropathy (IgAN),characterized pathologically by deposition of IgA complexes in the glomerular mesangium,is the most common form of primary glomerulo-nephritis in children and adolescents and is considered to be one of the important cause of end-stage renal failure.Abnormal immune factors become the research hotspot in recent years,but the specific pathogenesis has not yet been fully elucidated.Toll-like receptors are a family of receptors,which have been evolutionarily conserved to recognize pathogen-associated molecular patterns.Toll-like receptors do not only play an important role in activation of innate immune,but also regulate acquired immune,which is a bridge connecting the natural immune and acquired immune.Studies have shown that Toll-like receptors plays an important role in the process of occurrence and development of IgAN.This paper reviews its biological characteristics and the pathogenesis of IgA nephropathy.

Objective To establish a better and easier method for producing resveratrol and soybean isoflavone from polydatin and genistin by microbic metabolic enzyme. Methods The fungal strain Aspergillus niger S was fermented with solid medium. The enzyme isolated from Aspergillus niger S was purified by precipitation with ammonium sulfate and column chromatography with Q sepharose fast flow and superdex G200. The purified enzyme could be used to transform both resveratrol and soybean isoflavone. An analytical method for all the transformed products was established by the improved RP-HPLC. The HPLC separation was performed using a C18 250mm?4.6mm column and a gradient elution program. The eluent was acetonitrile/0.1% acetic acid in a flow rate of 1ml/min. The wavelength for ultraviolet detection was 280nm. Results The molecular weight of the enzyme isolated from Aspergillus niger S was about 165 kD, which was quite different from other reported glucosidases. The optimal temperature for this enzyme was 50℃, and the enzymatic activity decreased when the temperature dropped below 40℃ or elevated over 60℃. The optimal pH values were 5-10, and the enzyme would lose its activity if the pH was below 4. It was revealed by improved RP-HPLC that the products were polydatin and genistin at 27min and 39min, respectively, before enzyme treatment, while the transformed products at 45min and 64min after the enzyme treatment were resveratrol and genistein, respectively. Conclusions This purified enzyme isolated from Aspergillus niger S can transform both resveratrol and soybean isoflavone from low active form to high active form. It is a convenient and accurate method for the determination of resveratrol and soybean isoflavone. This method may be very useful in industry as well as clinical experimentation.

Congenital cataract accounts for 10％ of childhood blindness worldwide and is the second leading cause of childhood blindness in China.30％-50％ of congenital cataract patients are caused by heritability,and majority of them is autosomal dominant traits.In addition,autosomal recessive and X-linked fashion also are found to lead to congenital cataract.Up to now,more than 20 disease-causing genes and 100 mutation locus have been mapped to different chromosomal locations in the human genome using genetic linkage methods.The cataract-associated genes were classified as four categories,including crystallin gene,membrane protein gene,transcription factor regulatory gene and others,and they induce different phenotypes of cataract by different molecular mechanisms.To understand the disease-causing genes and their mechanism is helpful for the genetic diagnosis and treatment of congenital cataract.The current researching progress in molecular genetics of cataract is summarized in this review.

Only realizing part of work in fixed assets management department, hospital account of fixed assets management system is unable to integrate the equipment using,management and finance departments. While the bar code management system can merge three departments into one system and manage the fixed assets of hospital with consistency of accounts, card and equipment.

BACKGROUND: The nano-hydroxyapatite (NHA) biomaterials compounded with secondary phase or multiphase materials have been widely used in the domain of stomatology, and there have been several reports regarding the application of compounded NHA with composite resin. OBJECTIVE: To analyze the application progress of NHA in the domain of stomatology, and to prospect the potential significance of NHA in the stomatology materials. METHODS: Databases of Pubmed, EBSCOhost and China Journal Full-text were retrieved to screen out the articles. Inclusion crteria: ①The articles possess originality and dependable point and evidence. ②The articles have conclusive viewpoint and allround analyses. ③The agents of articles contact with this review closely.Exclusion criteria:repetition research and independent literatures with this review. And 24 literatures in accordance with the standard were selected in this study. RESULTS AND CONCLUSION: Many clinical application and animal experiments indicate that NHA has been extensively used in the oral surgery, endodontic, implantation and the other aspects, with the deeply research for combination of NHA and composite resin, NHA hopefully displays greater effectiveness in the stomatology materials.

Objective To study the diagnosis and treatment of acute gangrenous cholecystitis in senile (patients), so as to decrease complications and lower the mortality rate. Methods The clinical data of 112 senile patients with acute gangrenous cholecystitis were retrospectived analysed.Results 19 cases(17.0%) of the 112 patients had hyperpyrexia, 8(7.1%) had icterus,11(17.0%) had a mass in the right upper abdomen, 26(23.2%) had symptoms of peritonitis; 11(9.8%) were complicated with acute edematous pancreatitis, 5(4.5%) had acute obstructive suppurative cholangitis, 6(5.4%) had septic shock. All of the 112 patients underwent surgery. At operation, 79 of 95 cases of acute calculous cholecystitis had (gangrene) of gallbladder,16(16.8%) had gangrene with perforation; in 17 cases with acute non-calculous cholecystitis had gangrene of gallbladder, 9(52.9%) had gangrene with perforation. Of the 112 patients, 86 underwent cholecystectomy, 18 underwent partial cholecystectomy, 5 underwent cholecystectomy with bile duct exploration and T tube drainage, and 3 underwent cholecystostomy 104 patients(92.9%) were cured, 8 patients(7.1%) died, and 9(8.7%) of the 104 cured patients had postoperative complications. (Conclusions) Acute gangrenous cholecystitis in senile patients should be definitely diagnosed as soon as (possible), co-existent diseases should be correctly treated, emergency operation should be performed with in 24 hours of onset of symptoms(or in the shortest time after admission), and a suitable operation should be (selected).

Objective To study the method and efficacy of repairing cup ear deformity.Methods The mild cup ears were corrected by V-Y advanced flaps and retroauricular flaps(seventeen ears),and the severe cup ears corrected by rotate composite flaps of concha auriculae and retroauricular flaps(six ears).The donor defects were sutured in place in seven cases and repaired by skin grafting in six cases.From March 2000 to November 2005,sixteen cases(twenty-three ears),eleven patients were male and five female,with age range from eight to thirty-six years,were treated by the procedures mentioned above.Among them,twelve cases were mild cupping(five bilateral ears) and four cases severe cupping(two bilateral ears).Results All flaps survived with primary healing after operation.Follow-up from six months to two years showed that all the patients had achieved satisfactory appearance of external ear and obvious improvement of contour of auricle and cavity of concha.Conclusion V-Y advanced flap and rotate composite flap are effective methods for the correction of cup ear deformity.The rational using of retroauricular flap and fixation of contour of auricle and cavity of concha are also the key points for the operation.

Objective To investigate the clinical effect of the application of the clinical effect of the use of lamivudine and the safety in the treatment of infantile enteritis.Methods From August 2013 to May 2016 in our hospital for diagnosis and treatment of 112 cases of enteritis were retrospectively analyzed,according to the application in the treatment of antibiotics into ceftazidime group in 60 cases,cefotiam group 52 cases,two groups of children with rehydration,correct electrolyte disorders such as basic treatment,the difference of clinical effect comparison.Results After 3d treatment,ceftazidime group stool frequency was significantly lower than that of cefotiam group(P<0.05); ceftazidime group the duration of diarrhea,stool leukocyte recovery time were significantly lower than cefotiam group(P<0.05); three day,five day after treatment of serum CRP,PCT levels were ceftazidime group significantly lower than cefotiam group(P<0.05); the two groups of serum CRP and PCT levels before treatment were significantly decreased(P<0.05); After five days treatment,5D after treatment,ceftazidime group the effective rate of 58.33％,effective 41.67％,invalid rate 0％,cefotiam group the effective rate was 42.31％,effective 53.85％,invalid rate 3.85％,ceftazidime treatment group is better than that of cefotiam group(P<0.05).Conclusion In the treatment of infantile enteritis,the effect is reliable and the safety is high.

Objective To investigate the differences in expression of hypoxia-inducible factor-1? (HIF-1?) in placental trophoblasts between normal pregnancy and pregnancy-induced hypertension (PIH) and the relationship of HIF-1? expression with magnesium sulfate treatment of PIH. Methods Immunohistochemical streptavidin biotin-peroxidase complex technique (SABC) was used to examine the expression of HIF-1? in 20 cases of PIH and 20 cases of normal placenta. Three groups of PIH were divided according to the degrees of severity. The results were analyzed by computer-assisted imaging and compared by t-test. Results There was significant difference in the expression of HIF-1? between the PIH group and the control group (P