After our country enters WTO, confronted with the direct influence in big international market opening, editorial department will face fierce competition. Selecting the superior and eliminate the inferior will test every publishing house and every magazine directly. In order to improve the competition level, author has analyzed the current situation of China's periodical development, and then explored the scientific and technological periodical opportunity and challenge faced under the new situation.
China ; Periodicals as Topic ; economics

OBJECTIVETo study the effect of Astragali Radix on the denervated tibial muscle atrophy in rats, and discuss its mechanism.

METHODTotally 60 SPF-grade Sprague-Dawley rats were selected in the common peroneal nerve crush model, and then randomly divided into 6 groups: Astragali Radix high-dose, medium-dose, low-dose groups, the Mecobalamin group, the model group, and the sham operation group. They were administered with drugs after the operation. At 18 d, the pathological section staining and morphological analysis were performed. The wet-weight ratio and section area of tibial muscles were also measured. The real-time fluorescence quantification was adopted to detect the differential expression between Angptl4 and PI3K genes.

RESULT(1) Wet-weight ratio: The wet-weight ratio in Astragali Radix high-dose, medium-dose groups was much higher than that in the model group (P < 0.05 or P < 0.01). (2) Section area: The sham operation group was higher, with regular morphology; Whereas the model group showed significant decrease, with chaotic structure and obvious connective tissue proliferation; Astragali Radix groups and the mecobalamin group showed relatively small section areas, with chaotic structure and unobvious connective tissue proliferation. Compared with the model group, Astragali Radix groups showed significant increase (P < 0.01). (3) Motor end plate: The sham operation group was in uniform brownish black color and oval or round shape; Astragali Radix medium-dose and high-dose group and the mecobalamin group showed rough line edges; Astragali Radix medium-dose and low-dose groups and the model group showed decline in the number, with irregular morphology, rough line edges and a light color. (4) Angptl4 and PI3K: Compared with the model group, the Astragali Radix high-dose group showed significant increase (P < 0.05).

CONCLUSIONAstragali Radix has a significant effect in preventing and treating denervated tibial muscle atrophy. It may delay the muscle atrophy by increasing Angptl4 and PI3K gene expressions.

Angiopoietin-like 4 Protein ; Angiopoietins ; genetics ; Animals ; Astragalus Plant ; Astragalus membranaceus ; chemistry ; Dose-Response Relationship, Drug ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Gene Expression Regulation ; drug effects ; Male ; Muscular Atrophy ; drug therapy ; genetics ; pathology ; prevention & control ; Phosphatidylinositol 3-Kinases ; genetics ; Rats ; Rats, Sprague-Dawley ; Tibia

Carcinoma in Situ ; pathology ; virology ; Carcinoma, Squamous Cell ; pathology ; virology ; Diagnosis, Differential ; Female ; Humans ; Paget Disease, Extramammary ; pathology ; virology ; Papillomavirus Infections ; Precancerous Conditions ; pathology ; virology ; Vulvar Neoplasms ; classification ; pathology ; virology ; Warts ; pathology ; virology

Language ; Periodicals as Topic ; Publishing

OBJECTIVETo analyze the status and papers information of Chinese Journal of Applied Physiology, and provide a guide for authors, readers and editing staffs.

METHODSWe retrieved papers of Chinese Journal of Applied Physiology from 2009 to 2013 from CNKI, and performed a statistical analysis of papers information contained in the text using bibliometrics method. Papers information such as document type, found support, author affiliation type, and discipline classification, etc. was analyzed.

RESULTSSeven hundred and forty two papers including 27 full English articles in total were published in Chinese Journal of Applied Physiology from 2009 to 2013. More papers were supported by national, provincial, and the other foundation, it was about 82.6% of all papers.

CONCLUSIONChinese Journal of Applied Physiology has stable manuscript resources, various discipline papers, and play an important role for development of applied physiology in China.

Bibliometrics ; China ; Periodicals as Topic

ObjectiveTo study the reliability to asses s interstitial fibrosis in myocardium of advanced age spontaneous hypertension rats(SHR) by acoustic densitometry. MethodsFifteen 20-month-old spontanous hypertension rats and fifteen age matched Wistar Kyoto(WKY) as control group were included. Ultrasonic backscatter parameters were acquired at interventricular septum, left ventricular posterior wall, left ventricular lateral wall. Normalized values of integrated backscatter of left ventricular lateral wall were compared with results by stereological approach under eletronic and light microscope. Results Compared with control group, SHR group showed different integrated backscatter at interventricular septum, left ventricular posterior wall and left ventricular lateral wall(P

Objective To explore the application of acoustic densitometry(AD) on evaluating effects of simvastatin, probucol, captopril and Chinese medicine on the atherosclerosis (AS) characterization of rabbit. Methods Sixty male New Zealand rabbits were divided randomly into five groups:normal group, regression control group(RC), simvastatin group, probucol group, captopril and Chinese medicine group. The intima-media thickness (IMT), lumen acoustic densitometry (AII-L), intima-media acoustic densitometry (AII-I), adventitia acoustic densitometry (AII-A) and corrected acoustic densitometry (AIIc) of abdominal aorta were measured with high frequency ultrasound at the beginning, the 12th and 24th week of the experiment. Abdominal aorta was also examined through pathology at 24th week of the experiment. Results Analysis of abdominal aortas from 8 rabbits in vitro showed the different effects of the different frequencies of transducer on the AII value of lumen, intima-media and the adventitia. AIIc was calculated to evaluate acoustic density of intima-media to avoid the influence of individual divergence.AIIc in 4 drug-therapy groups were significantly higher than AS model group (P

Oyster protein was extracted from fresh oyster using salting-out method. It was determined by Kjeldahl procedure that the protein content was about 38. 6% and free amino-ni-trogen content of total nitrogen was about 21. 6%. Hydrolytic characteristic of oyster protein was analyzed by hydrolytic enzymes. SDS-PAGE results showed there were 4 types of protein zones. And the average molecular weight was 16172, 17633, 43227 and 57909, respectively.

Objective To evaluate the ventricula r septal defect qualitatively and quantitatively by real-time three-dimensional echocardiography (RT-3DE).Methods RT-3DE were performed in 10 pig hearts with 22 ventricular septal defects in multiple orientations and cross sections. The diameters and areas of ventricular septal defects were measured both in RT-3DE and anatomic view. Echo images and parameters derived by RT-3DE were compared with anatomic view. Results Technically satisfactory RT-3DE images were obtained in all preparations. The location, size, morphology, and spatial relationships with surrounding cardiac structure of ventricular septal defects were displayed instantly. No significant differences were found between RT-3DE and anatomic measurements. Conclusions RT-3DE has advantages of instantly displaying the ventricular septal defects in anyplane and has important surgical and interventional implications.

0.05 ), while defect dimensions by 2-D E were lower than those in surgical operation(P