Objective To screening myocardial Inflammatory gene and its function in myocardial injury after single bout of exhausted exercises.Methods Fifry male Sprague-Dawley rats were randomly divided into sedentary control groups and single bout of exhausted exercise groups.Rats were killed at 0,6,12 and 24 h after single bout of exhausted exercises,and the gene chip technology was used to screening myocardial inflammatory gene.Results After single bout of exhausted exercise,myocardial inflammatory gene mainly involved with Cc117, PF4, CCL2, CCL21b, CCL7, Cc112_predicted, I16r, I11r2, Ifngr, Ccr12 predicted,ICAM-1 and Adamts1,with a trend of inrease to decrease.The gene expression of Ce117, PF4, Cc112_predicted, I116r, I11r2,Ifngr,Ccr12_predicted and Adamtsl shgnificant significant apgrade at oh after exercise,and the gene expression of CCL2,CCL21b,CCL7 and ICAM-1 showed significant upgrade at 6h after exercise.Conclusions The significantly increase expression of the chemotaetic factors and their receptors,mediate inflammdtory cells to the ischemia myocardium might be the patho-mechanism of exercise-induced yocardial micro damage after single bout of exhausted exercise.

Intravenous immunoglobulin (IVIG) is safe and effective concentration formulation which is obtained from the plasma of health adults.Domestic IVIG was used in clinical since 1979 by Liao Qingkui,who are pediatric doctor form West China Second Hospital.Researches on clinical applications of IVIG are quickly developed at home and abroad,which now become the indispcnsable treatment for primary and secondary immunodeficiency diseases,autoimmune diseases,infectious disease(especially for virus),stem cell or organ transplantation and ICU areas.Treatment dose and course are administered accordding to individuals and pharmacokinetic characteristics of IVIG,immunoglobulin rose to peak of drug concentration quickly,and recovery to the original level of plasma after 3-4 weeks.Common therapeutic dose of IVIG recommended by the literature were 400mg/(kg·per time),3-5 d,or 1000 mg/(kg·per time),2d,or 2000 mg/(kg·per time),1d,which were usually used for idiopathic thrombocytopenic purpura,Kawasaki disease patients.Treatment perscription of 200-600 mg/(kg·per time),1 d,and once every 2-6 weeks are used for immunodeficiency diseases and infections.Now,the clinical application of IVIG was reviewed.

Objective To investigate the safety and feasibility of early removal of chest tubes after video-assisted thoracoscopic (VAST) lobectomy for lung cancer. Methods A retrospective study was performed based on the clinical data of sixty consecutive patients who underwent VATS lobectomy plus mediastinal lymph nodes dissection for lung cancer from October 2013 to September 2014 in a single center. Thirty patients were enrolled into the early removal management group (chest tubes removal when the drainage volume is less than 300 mL/d), while the other thirty patients were enrolled into the traditional management group (chest tubes removal at the drainage volume less than 100 mL/d ). Results Patients who underwent early removal management had a shorter time of chest tubes removal and postoperative hospital stay compared to the patients in the traditional management group [(2.10 ± 0.99) d vs. (3.83 ± 1.41) d, t = 5.485, P = 0.000; (7.97 ± 1.54) d vs. (9.20 ± 2.01)d, t = 2.669, P = 0.010)]. No statistically significant differences were observed among the drainage volume, postoperative complications and symptoms on fifteen days post-operation. No complication occurred on thirty days post-operation. Conclusions Early removal of the chest tubes after VATS lobectomy ,when the drainage volume is less than 300 mL/d,is safe and feasible. This leads to a shorter length of hospital stay without addtional risk of early postoperative complications.

OBJECTIVE:To investigate the status quo of the pharmaceutical care of drug consultation calls so as to facilitate the development of pharmaceutical care and promote rational drug use.METHODS:All the recordings on the pharmaceutical consultation calls from Jan.2001 to Sep.2008 were collected to analyze the compositions of consultants,problems consulted and the categories of the drugs consulted,etc.RESULTS:75.6% of the consultants were hospital employees;41.7% of the problems consulted were related to drug interactions and dosage and administration,and 22.8% of the drugs consulted were antibiotics.CONCLUSION:The drug consultation call model is convenient and feasible,and is proved to be a realistic professional pharmaceutical care.

Objective To form the basis of establishing a strategy for safe blood donor recruitment and to identify and subdivide the structures of donors.Methods Demographic data of blood donors, and blood testing results of HBsAg, anti-HCV, anti-HIV, and syphilis, from Nov. 2004 to Jun. 2005, were collected and analyzed.Results Among the 45270 voluntary blood donors, the total positive test rate were 3.11%, which was related to age, level of education and occupation of blood donors. About 76.7% of donors were young people, aged from 18 to 30. As the age increased, female donors were less than male ones. Among all the donors, 81.6% received higher education, which had lower positive test rate than those with lower level of education. Among the 45270 donors, farmers had the highest positive test rate; workers and service people came the second; students and medical staff had the lowest. As to donors with incomplete record, the positive test rate was higher than those with complete record.Conclusion During blood donor recruitment, quality control is important. People younger than 30, with student or higher education background, are relatively safe to recruit.

Objective To find out factors influencing voluntary blood donation activities and establish the most appropriate method for recruiting blood donors. Methods Questionnaire investigation of donors and data analysis by SPSS+ software. Results In Chengdu city, 2385 residents' knowledge on nonrenumerated blood donation was as follows: among the 2385 residents, the percentage of people who knew the government policies of blood donation was high (87.5%), but the percentage having common knowledge of blood donation was only 29.4%, of risk behavior was 32.5%. Among the 1349 participants who had donated blood, the percentages having common knowledge of blood donation and risk behavior were 33.4% and 38.1% respectively, compared to the 1036 participants who never donated (24.2% and 25.1%, respectively)(P

Integrating medical instruments with medical information systems becomes more and more important in healthcare industry. To make medical instruments without standard communication interface possess the capability of interoperating and sharing information with medical information systems, we developed a medical instrument integration gateway based on Integrating the Healthcare Enterprise Patient Care Device (IHE PCD) integration profiles in this research. The core component is an integration engine which is implemented according to integration profiles and Health Level Seven (HL7) messages defined in IHE PCD. Working with instrument specific Javascripts, the engine transforms medical instrument data into HL7 ORU message. This research enables medical instruments to interoperate and exchange medical data with information systems in a standardized way, and is valuable for medical instrument integration, especially for traditional instruments.
Equipment and Supplies ; Health Level Seven ; Humans ; Information Systems ; Software

It is now widely accepted that platelet aggregation plays an important role in physiological hemostasis and pathological thrombosis associated with cardiovascular and cerebrovascular diseases. Anti-platelet aggregation drug research is also a hot spot of current research. The biggest challenge of antiplatelet therapy has been the molecular overlap of the hemostasis and thrombosis, leading to a serious risk of bleeding. Recent studies have emphasized the importance of shear stress generated from blood flow, which will primarily drive platelet activation and aggregation in thrombosis. So if we can take advantage of the differences between the physiological and pathological vascular blood flow environment, the development of selective anti-platelet therapy may be a safer treatment for cardiovascular and cerebrovascular diseases. In this review, we discuss the underlying mechanisms of shear-induced platelet activation. Later, we summarize the effects and mechanisms of compounds and traditional Chinese medicine on shear-induced platelet activation. The aim is to provide a reference for the study of biological pharmacology of traditional Chinese medicine for promoting blood circulation and removing blood stasis.

Objective To study the cyfluthrin resistance and potential mechanisms of Anopheles sinensis in Nanchang Chang-bei International Airport,Nanchang City,Jiangxi Province. Methods The resistance levels of the local An. sinensis were de-tected by WHO drug resistance bioassay. During the bioassay,the dying mosquitos were classed as sensitive mosquitos,and the survival ones were classed as resistant mosquitos. The P450 monooxygenase activity and glutathione S-transferase activity were detected and compared between the two groups. At the same time,the death time of each sensitive mosquito was recorded,and the correlations between the death time and the P450 monooxygenase activity and glutathione S-transferase activity were ana-lyzed,respectively. Results The bioassay mortality of the local An. sinensis was 59.5%. The differences of the P450 monooxy-genase activities among the resistant mosquitos,sensitive mosquitos and laboratory sensitive mosquitos had statistical signifi-cances(F=151.89,P<0.01),the resistant mosquitos>sensitive mosquitos>laboratory sensitive mosquitos. The differences of glutathione s-transferase activities among the three groups had no statistical significance(F=0.72,P=0.49). There existed positive correlation between the mosquito death time and the P450 monooxygenase activity,and the regression equation was y=79.479+1.512x with the correlation coefficient of 0.88,while there was no correlation between the mosquito death time and the glutathione S-transferaseactivity. Conclusion The An. sinensis in Nanchang Changbei International Airport has been resistant to cyfluthrin,and the promotion of P450 monooxygenase activity maybe one of the reasons for the resistance.

Adrenal Hyperplasia, Congenital ; diagnosis ; Female ; Humans ; Infant, Newborn ; Male ; Neonatology ; Pregnancy ; Prenatal Diagnosis