Circulating tumor cells (CTCs) play an important role in tumor metastasis.Detections of CTCs are contribute to tumor treatment,which can provide reliable basis for predicting the prognosis and efficacy.CTCs are related to the staging and distant metastasis of non-small cell lung cancer (NSCLC).The number changes of CTCs are associated with the chemotherapy and radiotherapy effects and prognosis in NSCLC.Almost the same phenomena have been discovered in small cell lung cancer.In the future,CTCs may be used to monitor the occurrence of drug resistant tumor cells and help individual therapy for lung cancer.

Objective To explore the effect and mechanism of aerobic exercise on islet β-cells in type 2 diabetic rats.Methods Thirty healthy SPF 8-week old Wistar rats were randomly divided into control group (C, n=10), diabetic control groups (DMC) and diabetic exercise (DME) groups, 10 rats in each group, among which 7 successful rat models were used in the experiment.The diabetic rat model was established by high fat and sugar diet and i.p.injection of streptozotocin in a dose of 50 mg/kg.The rats of group DME were forced to perform 20 m/min running for 30 min, once a day, 6 days in a week, for 8 weeks.Other rats were allowed free movement.At the end of experiment, serum glucose and insulin were measured and homeostasis model assessment (HOMA) was calculated, and pancreatic tissue samples were collected for histopathological examination.The morphology and structure of pancreatic islets were observed under a digital microscope, the perimeter and area of islets were analyzed by image analysis, and shape factor of islets was calculated.The insulin content, glucokinase and ultramicro-ATPase activity in the pancreatic homogenate were determined.Results In the DME group, the perimeter and area of islets were significantly higher than the DMC group (P< 0.05), but still lower than the control group.The shape factor was significantly increased, the cell hypertrophy, vacuolization and nuclear pyknosis were markedly alleviated than those in the DMC group, the insulin content, glucokinase and the trace total ATP activity in the DME group were significantly higher than those in the DMC group (P<0.05), and the SF and HOMA were significantly changed.Conclusions Aerobic exercise can reduce the blood glucose level, improve the morphology of islets and β-cells in the type 2 diabetic rats.It may be due to increase of the activity of glucose kinase and ATP-synthase, and increased insulin sensitivity in the pancreas.

Objective To investigate the relationship of mental health and the personality traits of higher vocational college students. Methods Using CCSMHS and NEO-FFI,679 higher vocational College Students from 4 Hunan Vocational College were investigated. Results ①A few people of higher vocational college students had obvious psychological symptoms,and more people were in a state of sub-health. ②The personality trait of female higher vocational college students is different from that of the male significantly. There is no significant difference between different grades and different disciplines. ③There was a significant relation between the mental health of higher vocational college students and a number of personality traits. Conclusion The personality traits are the main factors influencing mental health of higher vocational college students.

Ear Diseases ; congenital ; Ear, Middle ; abnormalities ; Humans

Objective:To detect the levels of TGF-β1 in hypertensive renal function impairment patients and its clinical value. Methods: Primary hypertension patients with no target organs damage were enrolled in control group, primary hypertension patients with renal function stageⅠand stageⅡwere enrolled in renal function stageⅠgroup and stageⅡgroup. Serum TGF-β1 levels and renal function indices of three groups were compared. Results:There were no significant difference of serum creatinine and blood urea nitrogen of three groups(F=23.523, P<0.05);endogenous creatinine clearance rate of renal functionⅠandⅡgroups were lower than that of control group, TGF-β1 levels of renal functionⅠandⅡ groups were higher than of control group; TGF-β1 levels were negatively correlated with creatinine clearance rate. Conclusion:TGF-β1 level is an ideal index to reflect renal function for its level significantly changes in renal damage stageⅠ,Ⅱand has good correlation with Cin.

Objective To investigate the correlation between the characteristics of the computed tomography (CT) perfusion parameters and the expression of D2-40 with lymphatic vessel density (LVD) in cervical carcinomas.Methods A total of 42 patients with cervical carcinoma was divided into two groups with and without lymph node metastasis.Patients were evaluated with CT perfusion scan before operation.Monoclonal antibody D2-40 was used for immunohistochemistry to detect the LVD in the carcinoma tissue specimen.CT perfusion parameters and LVD of two groups were compared,and their relationship was analyzed.Results CT perfusion parameters including blood flow (BF),peak enhancement image (PEI),and blood volume (BV) in the lymph node metastasis group were significantly higher than those in the no lymph node metastasis group (t =-2.206,-2.29,-2.336,P ＜ 0.05).The time to peak (TTP) was significantly lower in the lymph node metastasis group than the no node metastasis group (t =6.908,P ＜ 0.01).The LVD in the lymph node metastasis group was significantly higher than the no lymph node metastasis group (t =-5.092,P ＜ 0.01).The CT perfusion parameters (BF,PEI,BV) and LVD of cervical carcinomas had a significantly positive correlation (r =0.65,0.56,0.61,P ＜ 0.01).The TTP and LVD had a significantly negative correlation(r =-0.55,P ＜ 0.01).Conclusions CT perfusion imaging and higher LVD help to diagnose the lymph node metastasis of a cervical carcinoma,and have important guidance role in the surgical options for cervical cancers.

OBJECTIVE:To observe the clinical efficacy of Shenkang injection,a new compound preparation for chronic renal failure(CRF).METHODS:A total of 84 CRF patients were randomly divided into two groups:the treatment group(n=54)was given routine antihypertensive therapy and infused(iv gtt)with Shenkang injection 60～ 100mL(dissolved in 10% GS 300mL or NS 300mL)q.d for 30d;the control group(n=30)was infused(iv gtt)with Compound danshen injection 250mL q.d for 30d.RESULTS:As compared with the control group,serum levels of creatinine and urea nitrogen(UN)in treatment group had a greater reduction(P

0.05) between the two group. Conclusions:No significant difference, concerning efficacy and toxicity ,is shown between domestic made and imported vinorelbine plus cisplatin combination chemotherapy in treating non small cell lung cancer.

Objective: To prepare 2 composites using different proportions of hydroxyapatite and collagen and to assess their structural and biological properties, so as to pave a way for preparing tissue engineering chest wall scaffold. Methods: Two kinds of hydroxyapatite/collagen composites were prepared according to the weight ratios of 1:1 and 1:2; collagen sponge served as control. Then the structures of the 2 composites and the collagen sponge were observed under SEM. In vivo study was conducted to assess the biocompatibility and biodegradation of the composites by gross inspection and histological examination. Results: The collagen sponge had a 3-D network structure with fluey collagen fibers and poor mechanical strength, and its structure was damaged within 2 weeks after implantation and was completely absorbed 4 weeks later. The hydroxyapatite and collagen were well mixed in the composite with a hydroxyapatite to collagen ratio of 1:2; the composite had homogeneous 3-D porous structure (size of the pore being 100-400 m) and showed good biocompatibility: maintained its porous structure 4 weeks after implantation and was absorbed within 8 weeks. In composite with hydroxyapatite to collagen ratio of 1:1, the hydroxyapatite particles were separated from collagen fiber and conglomerated into masses, and the composite resulted in severe tissue reaction after implantation. Conclusion: When mixed with a reasonable proportion of hydroxyapatite, the collagen sponge has improved structure, biodegradable performance, and biocompatibility; the composite may be a novel scaffold for tissue engineering chest wall reconstruction.

Objective: To prepare a novel surgery mesh made of polydioxanone(PDO) and to study its in vivo degradation properties and the related tissue reaction, so as to provide reference for future clinical application. Methods: PDO mesh was plain-woven and was coated with collagen and chitosan, then they were implanted into the dorsal muscle of rabbits for 2, 4, 8, 12, 16, and 24 weeks. Rabbits implanted with pure PDO mesh served as control. The degradation process of implanted materials, tissue-material interface, and the biological influence of the coating on the mesh were observed; tissue reaction was observed and evaluated by histological means and TEM. Results: Implantation of pure PDO mesh resulted in mild inflammation and fibrous tissues infiltrated into the mesh; degradation occurred during the 8th to the 12th week and degraded completely within 24 weeks; there was no denaturalization, necrosis, and abnormal hyperplasia in the surrounding tissues. Collagen coating stimulated a fibroblastic proliferation and was reabsorbed within 4 weeks; and the naked mesh was infiltrated by fibrous tissues. The chitosan coating showed a strong inhibitory effect on the growth of the surrounding connective tissues. Conclusion: The biodegradable polydioxanone mesh has an excellent biocompatibility, a friendly tissue-material interface and a proper degradation rate matching to the tissue regeneration rate. The biological characteristics of the mesh can be modified by different coatings, making it a very promising degradable surgical mesh.