Neuropathic pain is the clinically common disease and does great harm to physical and mental health.Because its pathogenesis is still unclear and there aren′t effective therapeutic tools and the course of chronic pain lasts a long time,the study of neuropathic pain has become the hot spot and the focal point of pain research area.This article reviews various kinds of animal models of neuropathic pain,which can repeat neuropathic pain symptoms of mankind and may be effective ways to make research.By acting on P2X receptors,ATP is the signal of an important transmitting pain.By utilizing animal models of neuropathic pain,it shows that P2X receptors have an important role in the formation,transmission and regulation of pain and may be a new target of neuropathic pain therapy.

Oxymatrine is the main effective monomer of Radix Sophorae flavescentis( Kushen) , which has a variety of pharma-cological actions and valuable clinical applications. Recently, there are many reports about molecular mechanisms of oxymatrine pharmacological actions, giving highly attention to the anti-in-flammatory, anti-fibrosis and antineoplastic effects. These effects are achieved through resisting of oxidation and free radical, anti- ＜br> virus, and affecting the secretion of inflammatory factor and ap-optosis and so on. This article summarizes the reports on the mo-lecular mechanism of the protection on liver, cardiovascular sys-tem, endocrine system and nervous system, hoping to provide theoretical basis for the practical application.

Objective To discuss the application effect of TCM constitution recognition on TCM chronic disease refining health management for type 2 diabetes mellitus.Methods Totally 140 outpatients with type 2 diabetes mellitus from Chaoyangmen Community Health Center were selected and divided evenly into two groups after TCM constitution assessment. The study lasted for 2 years (1st August 2013–31st July 2015). The control group (70 cases) received diabetes community health management. In addition to routine community health management, TCM constitutional management was also administrated in the intervention group. The latter group received medicine, food, sport guidance according to the results of TCM constitution assessment. The blood glucose, blood lipid, BMI and waist-hip ratio of the two groups were monitored, and the self-management and life quality of patients were evaluated. Results After the intervention, significant improvement was found for fasting blood glucose, 2 h postprandial blood glucose, BMI, waist-hip ratio and blood lipid both in the control group and the intervention group, and the improvement in the intervention group was more obvious than the control group (P<0.05). The whole level of self-management and life quality was greatly increased in both groups, while improvement of the intervention group was much higher than the control group (P<0.05).Conclusion Integrated application of TCM constitutional theory in TCM chronic disease refining health management for type 2 diabetes mellitus will contribute to improving patients’ self-management, changing their clinical symptoms and finally improving their life quality.

Enterostomy are a common surgical procedure in the treatment of rectal cancer, has more than 100000 each year in our country requires enterostomy patients, whereas patients with postoperative rehabilitation and nursing leave the family caregivers, the study found that with baseline levels of care for people, and increased mortality 63% of caregivers within five years, attaches great importance to the caregivers of patients with life can avoid the occurrence of another patient, at the same time also can improve the quality of family care, reduce the demand for medical and waste. Therefore, this article will from the permanent patients underwent colostomy care burden of family caregivers, health education needs, psychological burden to understand colostomy caregivers are faced with the problem of our country, explore the mental and physical health of the caregivers related factors, provide the basis for effective follow disease intervention.

LL-37 is the only member of the cathelicidin family known to exist in human acting as an endogenous antibiotic.After removal of the highly conserved N-termini of precursor hCAP18,a 15 000 C-terminal fragment called LL-37 bearing all of the known biological acivity hitherto is liberated by proteinase.LL-37 is active against both Gram-positive and Gram-negtive bacteria.Its antibacterial activity depends on the formation of ?-helical conformation.The cytotoxic activity of LL-37 is inhibited by apolipoprotein A-Ⅰ(apoA-Ⅰ) in the plasma,thereby it can function as a scavenger of LL-37 and prevent host cell damage.In addition to its microbicidal activity,LL-37 has several other functions such as chemotactic,angiogenic action and the ability to bind and neutralize the endotoxin.

Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

Cytokine induced killer (CIK) cells are the main immune cells for tumor immnuotherapy.Relevant basic researches show that CIK cells have significant antitumor effects for various hematologic tumor and solid tumors.Clinical studies confirm that CIK cells-based therapy have exact therapeutic effect and mild adverse reaction for tumor patients.So CIK cells-based therapy is a promising and safe method and it is important to establish standards for cultivating and using CIK cells.

ObjectiveTo investigate the effects of alpha-lipoic acid on serum soluble intercellular adhesion molecule-1 (sICAM-1) and high sensitive-C reactive protein (hs-CRP) levels in patients with early diabetic nephropathy (DN).MethodsA total of 61 patients with early DN were randomized into treatment group (n =31 ) and control group ( n =30 ).The two groups were both treated with dietary control and oral hypoglycemic drugs or insulin.Meanwhile,the treatment group was additionally given alpha-lipoic acid 300 mg/d by intravenous infusion for 20 days.The levels of sICAM-1 and hs-CRP in each group were detected.Results The levels of sICAM-1 and hs-CRP were decreased significantly in alpha-lipoic acid treatment group after 20 days of therapy ( [ 198.03 ±23.67] μg/L vs [271.17 ±34.66] μg/L,[5.16 ±0.43] mg/Lvs [7.95 ±0.88]mg/L,P ＜0.01 ).Conclusion alpha-lipoic acid may decrease the expression of slCAM-1 and hs-CRP,and that may be one of the mechanisms to postpone the progress of early DN.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

Objective To explore the relationship of coping style and social support with hope in maintenance hemodialysis patients.Methods A total of 177 maintenance hemodialysis patients were recruited from 3 dialysis centers in Guangzhou and were investigated with the self-designed questionnaire,the Herth Hope Scale,Simplified Coping Scale and Social Support Scale.Structural equation model was used to establish the model of relationship of coping style and social support with hope level.Results The average score of hope in maintenance hemodialysis patients was (31.83±3.69) points.The total score and all the sub-scores of social support were (42.24±4.09) points,(23.72±2.36) points,(10.82±2.26) points,(7.70±1.36) points respectively.And the average scores of positive coping style and the negative coping style were (2.15±0.28) points,(1.56±0.33) points,respectively.Social support had direct positive effect on hope level,and the positive and neg-ative coping style had indirect positive and negative effect on hope level respectively.Conclusions Social support has a direct effects on hope level in maintenance hemodialysis patients.Positive and negative cop-ing styles have indirect effects on hope level through social support.