The epidermal growth factor receptor ( EGFR)-small molecule tyrosine kinase inhibitor (TKI) has become the focus of clinical trials for first-line treatment of advanced non-small cell lung cancer ( NSCLC). Though TKI and concurrent chemotherapy show no advantage for survival, the use of TKI in combination with chemotherapy ( sequenced or maintenance) prolongs progression-free survival. In those patients with EGFR mutation, TKI as monotherapy in first-line setting has a significant effect on progression - free survival compared with chemotherapy. These data shows that EGFR mutation is effective predictor for TKI treatment.

Objective To observe the effect of local curettage combined with ointment in fistula applying in refractory apical fistula.Methods 56 cases of patients with refractory apical fistula one month after faultless root cannal therapy divided into group A and B by lottery,with 28 cases in each group.Local curettage combined with ointment in fistula applying in group A,and no processing applying in group B.One year fellow up was given to every patient to compare the effect between the two groups.Results All the 28 cases of patients with refractory apical fistu-la were recovering in three months.The improvement rate was 100%,recovery rate 100%,and the mean time of fistula disappearing was (0.5 ±0.1)months,time of shadow around root tip disappearing (2.5 ±0.7)months.Four cases in group B were improved in three months,three cases in six months and one case in nine months,with two cases recove-ring.The improvement rate was 28.57%,recovery rate was 7.14%,and the mean time of fistula disappearing was (5.0 ±0.7)months,time of shadow around root tip disappearing (9.4 ±1.0)months.The improvement rate in group A was higher than that in group B (χ2 =33.749,P<0.05),recovery rate in group A was higher than that in group B (χ2 =49.727,P<0.05).The mean time of fistula disappearing in group A was shorter than that in group B (t=3.081,P<0.05),mean time of shadow around root tip disappearing in group A was shorter than that in group B (t=5.042,P<0.05).Conclusion It was effective to apply local curettage combined with ointment in fistula applying in refractory apical fistula,and it is worthy of clinical popularization.

Circulating tumor cells (CTCs) play an important role in tumor metastasis.Detections of CTCs are contribute to tumor treatment,which can provide reliable basis for predicting the prognosis and efficacy.CTCs are related to the staging and distant metastasis of non-small cell lung cancer (NSCLC).The number changes of CTCs are associated with the chemotherapy and radiotherapy effects and prognosis in NSCLC.Almost the same phenomena have been discovered in small cell lung cancer.In the future,CTCs may be used to monitor the occurrence of drug resistant tumor cells and help individual therapy for lung cancer.

Humans ; Polymorphism, Genetic ; Pulmonary Fibrosis ; genetics ; physiopathology ; Transforming Growth Factor beta ; genetics ; physiology

AlM: To understand the prognostic factors of myopic rhegmatogenous retinal detachment ( RRD) surgery, and provide guidance for clinical treatment.
METHODS: This retrospective study included 288 myopic RRD eyes ( excluding trauma, aphakia, congenital diseases, tractional and exudative retinal detachment ) from April 2007 to April 2014 in our department. The surgical anatomic reduction situation was statistically recorded, the effects of hole's size, retinal detachment duration on sight restoration and surgery success rate were analyzed
RESULTS:The statistical results showed that 245 eyes (85. 1%) healed after their first operation, about 95. 1%cases finally healed after their second or third operation. The degree of myopia and aging factor influenced the RRD operation:the higher degree and the older age were, the lower success rates were (P<0. 05). Through surgery, postoperative visual acuity was significantly better than the preoperative ones ( P<0. 01 ). A higher degree of myopia RRD led to poor visual acuity ( P < 0. 01 ). Postoperative visual acuity of 152 eyes ( 52. 9%) were improved, of which 106 eyes' visual acuity was 0. 05 or more.
CONCLUSlON:Surgery is an effective means of treating myopic RRD. The older patients have lower success rates. A higher degree of myopia led lower reset rate after retinal detachment surgery and poor postoperative visual acuity. When treat myopic RRD, we should pay attention to the recovery of visual function.

Objective:To determine the effects of silencing glucose regulated protein ( GRP94 ) on the proliferation and apoptosis of breast carcinoma MCF7 cells. Methods:Chemically synthesized siRNA targeting GRP94 gene and transfected into MCF7 cells used by Liopfectamine RNAIMAX. The mRNA and protein expression levels of GRP94,cyclinD1,Bax and Bcl-2 were detected by Real-time PCR and Western blot. CCK8 assay was used to detect the effect of specific GRP94 siRNA on cell proliferation and the effect on cell cycle and apoptosis were analyzed by flow cytometry and Hoechst 33258 staining. Results:Compared with the siRNA-NC cells, the expression of GRP94 was significantly down-regulated in MCF7 cells. Knockdown of GRP94 in MCF7 cells decreased cell proliferation and promoted cell apoptosis. The expression of cyclinD1and Bcl-2levels were significantly reduced, and Bax level was increased in siRNA-GRP94 MCF7 cells. Conclusion: The siRNA-mediated GRP94 silence significantly inhibits MCF7 cell proliferation,promoted cell apoptosis by down-regulating cyclin D1,Bcl-2 expression and up-regulating the Bax expression in MCF7 cells.

Humans ; Platelet-Derived Growth Factor ; Pulmonary Fibrosis

Animals ; Humans ; Interleukin-1 ; physiology ; Mice ; Pulmonary Fibrosis ; physiopathology ; Rats ; Receptors, Interleukin-1 ; physiology

In this paper, water content, mineral element and active ingredient concentration on fifteen Inonotus obliquus which comes from all over the world were studied. In the aspect of water content, the free water content of Finnish birch was the highest, reaching 77.21%, significantly better than that of other strains. The free water content of JL04 is the lowest, only 54.6%. The bound water content of HLJ01 which from Heilongjiang is the highest, reaching 10. 74% , significant differences among other strains. The bound water content of Birch Russia was the lowest. In the aspect of mineral element, the calcium content of NBRC9788 was the high- est (3.49 mg · g(-1)), significantly better than other strains. The second was Finnish birch. The lowest was CX02. The phosphorus content of NBRC9788 was the highest (210.12 μg · g(-1)), significantly superior to other strains. The lowest was JL04. In the aspect of active ingredient concentration, the triterpenoids content of HLJ01 was highest (23.7 mg · g(-1)), significantly better than other strains. It was good strains for biological products research and development and then was Finnish birch. The betulin content of MAFF420165 and MAFF420308 was low, they were not suitable for production. The polysaccharide content of Finnish birch was the highest (9.7%), significantly better than the other strains, it is one of the most ideal and good strains to develop polysaccharide. The polysaccharide content from MAFF420308 and MAFF420256 was 1.2%, lower than other strains. We suggest that avoid using these strains in the study of polysaccharide product development.
Basidiomycota ; chemistry ; classification ; Calcium ; analysis ; Minerals ; analysis ; Phosphorus ; analysis ; Polysaccharides ; analysis ; Water ; analysis

Objective To study the efficacy and safety of intravenous and intra-arterial thrombolytic therapy in acute cerebral infarction(ACI).Methods 65 patients with ACI were randomly divided in two groups:intra-arterial thrombolytic group (n=21) were intra-arterially administed with 250 000-500 000 units urokinase through femoral artery and intravenous thrombolytic group (n=44) were intravenously administed with 1.5 million units urokinase.Before and after treating, their neurological deficit scales were evaluated. The thrombin time (TT), prothrombin time (PT) and fibrinogen were detected before and after thrombolysis 6 h,3 d and 7 d.The CT scan was rechecked after thrombolysis 24 h.Results (1)ESS scales were higher in the intra-arterial infusion group than those in the intravenous infusion group at 2h, 6h, 24h, and 3d (all P