Pulsed radiofrequency (PRF) which can form high voltage around the tissues by emitting pulsed electric current may affect activity of synapses and cytokine. It has many advantages, such as smaller trauma, easier manipulation, safer, better effect, and so on.

Animals ; Cell Culture Techniques ; Cell Differentiation ; Cell Proliferation ; Embryonic Stem Cells ; cytology ; metabolism ; Germ Cells ; cytology ; metabolism ; Germ Layers ; cytology ; metabolism ; Humans ; Induced Pluripotent Stem Cells ; cytology ; metabolism ; Mice ; Pluripotent Stem Cells ; cytology ; metabolism ; Reproductive Techniques, Assisted

Congenital cataract accounts for 10％ of childhood blindness worldwide and is the second leading cause of childhood blindness in China.30％-50％ of congenital cataract patients are caused by heritability,and majority of them is autosomal dominant traits.In addition,autosomal recessive and X-linked fashion also are found to lead to congenital cataract.Up to now,more than 20 disease-causing genes and 100 mutation locus have been mapped to different chromosomal locations in the human genome using genetic linkage methods.The cataract-associated genes were classified as four categories,including crystallin gene,membrane protein gene,transcription factor regulatory gene and others,and they induce different phenotypes of cataract by different molecular mechanisms.To understand the disease-causing genes and their mechanism is helpful for the genetic diagnosis and treatment of congenital cataract.The current researching progress in molecular genetics of cataract is summarized in this review.

[Objective] To study the availability of machine packaging Chinese yam.By comparing granules in different specifications of Chinese yam,select best granule diameter,replace the primary artificial package with machine for large batch production.[Method] Measure water soluble extract and humidity-absorption,pile concentration and dormant angle,investigate its fill and flow.[Result] The measurement of 10order≤d1≤24order is more in accordance with experimental requirement.[Conclusion] The granule of 10order≤d1≤24order has more availability for machine package.

In order to investigate the feasibility of serum creatine kinase (CK) and blood urea nitrogen (BUN) in monitoring pre-competition training of badminton athletes, the pre-competition training load of 20 badminton athletes was studied, and serum CK and BUN were determined before, immediate and next morning after training. The results showed that after intensive training for one week, serum CK levels were significantly increased by 57.53 mmol/L (P<0.05). After regulation of the training intensity, average serum CK levels were increased by 21.79 mmol/L (P<0.05). BUN contents were increased by 0.83 mmol/L on average with the difference being not significant (P>0.05). After intermittent training, there was significant difference in the average increased levels of serum CK in athletes (P<0.05). There was significant difference before and after regulation of training (P<0.05). The increased levels of BUN were 0.78 mmol/L without significant difference (P>0.05). It was concluded that serum CK was one of the biochemical indicators monitoring the training load sensitivity of badminton athletes, but BUN was of little value in monitoring the training load. Both serum CK and BUN recovered slowly after one-week intensive training and intermittent training, suggesting the metabolic mechanism of human body in training needs further study.

Objective To construct dendritic cell(DC) vaccine expressing hepatitis C virus(HCV) multi-cytotoxic T lymphocyte(CTL) epitopes,which can stimulate T-cell response in vitro,providing certain information for immunization experiments in vivo for the next step.Methods DCs were infected with recombinant defective adenoviruses expressing two HCV CTL epitopes with tagged green fluorescent protein(GFP).The rate of infection was detected by the fluorescence microscope or flow cytometry;the expression of multiple CTL epitopes in DC was proved by RT-PCR and Western Blot.The cell surface markers of DC such as CD80,CD83,CD86 and HLA-DR were identified by flow cytometry.T cell proliferation effect promoted by DC was observed by cell counting kit-8(CCK-8).IL-12p70 in DC culture supernatant and IFN-γ in T cell supernatant were detected by Enzyme-Linked Immuno Sorbent Assay(ELISA).HCV specific CTL activity was measured by LDH release assay.Results Recombinant multi-CTL epitopes and GFP were successfully expressed in DC.Adenovirus can promote DC maturation,the percentages of CD80,CD83,CD86 and HLA-DR were(71.19±3.29)%,(81.21±5.07)%,(91.23±4.24)%,(97.95±5.31)% respectively.Infected DC promotes homologous T cell proliferation and the stimulation index was 6.806±0.247 when DC:T was 1:10.The secretion of IL-12 and IFN-γ was also increased to(193.83±6.25) pg/ml and(111.14±2.09) pg/ml respectively.The CTL stimulated by infected DC could specifically kill Huh-7.5 cells transfected with FL-J6/JFH transcripts.The cytotoxicity was 35.99% when the rate of effector to target was 100:1.Conclusion The multi-CTL epitopes recombinant adenovirus can effectively infect DC in vitro,promoting the T cell immune response,laying the foundation for developing anti-HCV DC vaccine.

No abstract available.
Humans ; Quality of Life* ; Self Care*

Objective To research the treatment of cervical HR-HPV infection by vaginal douching with eight kinds of antiviral herbs such as Radix Arnebiae and patrinia.Methods 42 patients with 13 types of cervical HR-HPV infection were selected from Outpatient.27 patients were recruited into a treatment group and 15 patients were recruited into a control group voluntarily.The treatment group was applied with antiviral herbs and the control group was applied with policresulen vaginal suppositories for 3 courses (one course for 7 days per month).The clearance rate and loading dose of 13 types of cervical HR-HPV were observed.Results The negative conversion ratio of the treatment group and the control group was 88.5％ and 15.4％ respectively,and the total efficiency was 100％ and 46.2％ in the treatment group and the control group respectively.The total efficiency between the two groups were significantly different(x2=17.062,P＜0.01).Conclusion The therapeutic effect of antiviral herbs for cervical HR-HPV infection was definite and with no side reactions.

Objective To summarize the experience of nursing patients undergoing off-pump coronary artery bypass grafting. Methods Fifity patients undergoing off-pump coronary artery bypass grafting were nursed by preoperative measures including metal nursing, blood pressure management, oxygen therapy, training of respiratory function, nursing of prepared bloods and postoperative ones including monitoring of cardiovascular and respiratory systems, medication of vasoactive agents, nursing of drainage tubes, monitoring of blood sugar and sustaining of electrolyte balance.Results Among the 50 cases, 6 had infections of incisional wound and 5 had the complication of rapid atrial fibrillation, all improved after medication. All other patients were cured and discharged except two deaths. No nursing-related complications occurred.Conclusion The off-pump coronary artery bypass grafting is highly risky and perioperative delicate nursing is critical for the success of the operation.