Objective To construct a cDNA expression library of Humulus pollen and provide the basis for screening the major allergenic components and producing recombinant allergen of Humulus pollen.Methods Verdure Humulus pollen were collected and preserved in liquid nitrogen after being sift out.Total RNA was extracted from the Humulus pollen with trizol reagent,and cDNA was synthesized by RT-PCR with purified total RNA.Then the cDNA was digested by SfiⅠ and the fragments smaller than 400bp were removed by chroma spin-400 column,and the fragments longer than 400bp were ligated with ?TripIEx2 Vector.The library was packaged in vitro and a small portion of packaged phage was used to infect E.coli XL1-Blue MRF′ for titration.The diversity of the library and the length of the inserted fragments were analyzed by PCR.Results The cDNA expression library contained 5?10~(5) recombinants and the percentage of recombination was 90%.The average length of inserted cDNA fragments was about 1.02kb.Conclusion The constructed cDNA expression library contains appropriate contents and sizes of cDNA fragments and is qualified for screening target cDNA clone.

Objective:To investigate the sexual concept and sex status of pregnant women so as to instruct pregnant health care and improve living quality.Methods:112 random sample candidates who have mature fetus for delivery in hospital were chosen to investigate and analyze by means of self-edited questionnaires and Eysenck protracted sexual latitude scale test.Results:Both sex frequency and sex continuing time of pregnant women reduced remarkably;their knowledge on sex related to pregnancy was generally poor;average value of Eysenck protracted sexual latitude scale test was 5.25,lying below the average points;professionals and individual professionals have statistics differences in occupation team;the single-mode of sexual intercourse in the sex of pregnant women has the highest percentage.Conclusion:Pregnant women lack the related health care knowledge on sex,and their concept of sex is comparatively conservative,so they can not enjoy full sex and need further improvement.

Objective:Under the background that the army hospitals need to employ civilian nurses in a large quantity,we try to grope a scientific,objective method to choose the nurse who matches post.Methods:Referring to related data and the ritual of talented person evaluation,we chose the evaluation methods including paper test,computer test,personal interview and data integration;arranged the steps of planning,assigning the weight,performing evaluation,integrating the test results,and tracking for feedback;in order to form the mode to test the employed nurses' occupational diathesis.Results:We fulfilled a series of scales for mood and hobby,weight score,quality structure,evaluation method comparison and nurse's professional quality evaluation,etc,and determined the evaluation methods and procedures.Conclusion:Evaluating more than 100 nurses who are newly employed using this new mode,the result demonstrates that this evaluation mode can be easily operated,and is a guide in employing quantities of professional nurses for a hospital.

Objective:To study the clinical significance of central electric-fetal monitoring system for ante-partum observation and measurement.Methods:The experimental group (253 pregnant women who delivered in our hospital from January,2001 to August,2003) was monitored with the central electric fetal monitoring system.The control group (250 pregnant women who delivered the children in our hospital from July,1998 to September,2000) was not monitered with this system.Then,the results of fetal monitoring and the outcome of prenatal fetuses of the two groups were compared and analyzed.Results:The rate of abnormal FHR was markedly higher in experiment group(30.8%) than in the control group(17.2%),and the rate of variable deceleration in abnormal graph was markedly higher in the experimental group (28.8%) than in the control group (12.4%).There was a significant difference is significant between the two groups.The incidence of asphyxia among newborns was lower in the experimental group (4%) than in the control group (8.4%),and the difference is significant between the two groups.However,there was no significant difference in the rate of cesarean delivery and Simpson forceps delivery between the two groups.Conclusion:Ante-partum monitoring assisted by central electric-fetal monitoring system can improve the prenatal prognosis and increase the rate of cesarean delivery.

Objective: To examine the effect of group mental intervention on implicit and explicit aggression of violent juvenile criminals. Methods: A survey investigation and IAT test on implicit aggression were employed to evaluate the aggression of violent juvenile criminals. And the effect of ten times of group intervention on aggression of juvenile criminals was analyzed. Results: After group mental intervention, the implicit aggression was not reduced significantly, but the intervention effect on explicit aggression was significant. Physical aggression and verbal aggression of explicit aggression reduced significantly, but there was no significant difference in rage and hostility. Conclusion: Group mental intervention is an effective method for reducing explicit aggression of violent juvenile criminals.

The striatum is involved in diverse behaviors which depend on intact dopaminergic innervation. Recent electrophysiological studies have revealed that dopamine alters both voltage dependent conductances and synaptic transmission, resulting in state dependent modulation of target cells. This review makes clear predictions about how dopamine should alter the responsiveness of striatal neurons to extrinsic excitatory synaptic activity.

Objective To construct a cDNA expression library of psilgramma menephorn and provide the basis for screening the major allergen and producing recombination allergen of psilgramma menephorn. Methods Total RNA was extracted from the whole body of psilgramma menephorn with TRIZOL mRNA purified with Oligo (dT) Spin-Column. ds cDNA was synthesized through reverse transcription. EcoRI adapters were ligated to the blunt ends and the ends were phosphorylated. The XhoI digestion released EcoRI adapter. The fragments smaller than 400 bp were removed by GHROMA SPIN-400 column and the fragments longer than 400 bp were ligated to the Uni-ZAP XR vector. The lambda library was packaged in vitro and is plated on the E.coli cell line XL1-Blue MRF. The content and recombination rate of cDNA library were tested. The length of the inserted fragments was analyzed by PCR. Results The cDNA expression library contained 5?10 5 recombinants and the recombination rate was 67%. The average length of inserted cDNA fragments was about 1.49 kb. Conclusion The constructed cDNA expression library contains appropriate contents and size of cDNA fragments for screening target cDNA to clone.

Objective To study the method and efficacy of repairing cup ear deformity.Methods The mild cup ears were corrected by V-Y advanced flaps and retroauricular flaps(seventeen ears),and the severe cup ears corrected by rotate composite flaps of concha auriculae and retroauricular flaps(six ears).The donor defects were sutured in place in seven cases and repaired by skin grafting in six cases.From March 2000 to November 2005,sixteen cases(twenty-three ears),eleven patients were male and five female,with age range from eight to thirty-six years,were treated by the procedures mentioned above.Among them,twelve cases were mild cupping(five bilateral ears) and four cases severe cupping(two bilateral ears).Results All flaps survived with primary healing after operation.Follow-up from six months to two years showed that all the patients had achieved satisfactory appearance of external ear and obvious improvement of contour of auricle and cavity of concha.Conclusion V-Y advanced flap and rotate composite flap are effective methods for the correction of cup ear deformity.The rational using of retroauricular flap and fixation of contour of auricle and cavity of concha are also the key points for the operation.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

OBJECTIVE:To observe the effects of Xingnaojing injection on the efficacy and safety of patients with acute cere-bral infarction merge blood stasis syndrome. METHODS:70 patients with acute cerebral infarction merge blood stasis syndrome were randomly divided into observation group(35 cases)and control group(35 cases). Control group received conventional treat-ment according to 2014 edition of Chinese guideline for diagnosis and management of acute ischemic stooke. Patients within throm-bolysis time window were given intravenaus infusion of rt-PA,and those out of thrombolysis time window orally received 0.3 g As-pirin tablet every evening,then 0.1 g after 1 week+40 mg Atorvastatin calcium tablet every evening,and if patients have arfficulty suallowing,gavage administiation was implemented. Observation group additionally received 30 ml Xingnaojing injection,adding in-to 250 ml 0.9% Sodium chloride injection,intravenous infusion,once a day. The treatment course for both groups was 14 d. Clini-cal efficacy,γ-interferon(IFN-γ),interleukin-13(IL-13),interleukin-4(IL-4),high-sensitivity C-reactive protein(hs-CRP)lev-els,the US National Institutes of Health Stroke Scale(NIHSS)rating,improved Barthel index(BI),infarction size before and af-ter treatment and the incidence of adverse reactions in 2 groups were observed. RESULTS:There was no significant difference in the total effective rate and incidence of adverse reactions in 2 groups(P>0.05). Before treatment,there were no significant differ-ences in IFN-γ,IL-13,IL-4,hs-CRP levels,the NIHSS rating,BI and infarction size in 2 groups(P>0.05). After treatment,the IFN-γ,hs-CRP levels,the NIHSS rating and infarction size in 2 groups were significantly lower than before,and observation group was lower than control group,IL-13,IL-4 and BI were significantly higher than before,and observation group was higher than control group,the differences were statistically significant (P<0.05 or P<0.01). CONCLUSIONS:Based on conventional treatment,Xingnaojing injection shows obvious efficacy the efficacy and safety of patients with acute cerebral infarction merge blood stasis syndrome,it can reduce the levels of inflammatory cytokines,improve neurological deficits,daily living skills and in-farct volume,with good safety.