OBJECTIVE: To study the compositions and contents of Amino Acids in the Fruit of Eucalyptus globulus Labill. . METHODS: The contents of total Amino Acids and free Amino Acids in the Fruit of Eucalyptus globulus Labill. were determined with Amino Acid analyzer. RESULTS: The content of total Amino Acids in the Fruit of Eucalyptus globulus Labill. was 4. 76g, among which that of the Amino Acids essential for human body was 2. 11g ( accounting for 44. 3% ) ; the content of free Amino Acids was 25. 29mg, among which that of the Amino Acids essential for human body was 9. 3mg( accounting for 36. 7% ) . CONCLUSIONS: The Fruit of Eucalyptus globulus Labill. is rich in Amino Acids, and is of high nutritional value.

The quasispecies nature of hepatitis B and C virus (HBV, HCV) plays an important role in the pathogenesis, immune escape and drug resistance during chronic infection. Although there is still a lack of effective treatment for hepatitis C, a series of nucleoside analogs (NA) have been developed for the treatment of hepatitis B. NA resistant HBV mutants can accumulate during prolonged therapy and lead to the failure of anti-HBV therapy. Switching to other sensitive NAs can inhibit the emerged resistant mutants. Therefore, understanding the evolution of viral quasispecies under drug pressure is crucial for the establishment of antiviral strategy and the monitoring of antiviral process. Immune response and escape are complicated process, during which both host and virus factors may play their roles. Further understanding of the interaction and interrelationship between host and these viruses may lead to optimized prevention, diagnosis and treatment for chronic hepatitis.

s Objective:To study the expression of nucleolar orga ni zer region associated protein (Ag NORs) of T lymphocytes in the patients with tumor of salivary gland. Methods:The expression of Ag NORs of T lymphocytes in 60 normal adults, 56 patients with benign salivary gla nd tumor and 45 patients with malignant salivary gland tumor was analysed by Ag stainning and KL imaging system. Results: The ratio of Ag NORs positive area to nuclear area of T lymphocytes in normal adults,pati ents with benign tumor and those with malignant tumor were 7.88?0.10, 5.71?0.1 3 and 4.21?0.12 respectively ( P

Objective To construct the uItrasound microbubbIes co-carrying recombinant adenovirus containing stromal cell derived factor 1 (SDF-1α) and bone morphogenetic protein 2 (BMP2),and to study the maximum efficiency of carrying adenovirus and the optimum proportion of double gene combined with ultrasound contrast agents.Methods Microbubbles were combined separately with recombinant adenovirus co-expression of enhanced green fluorescent protein and SDF-1α(pAd-EGFP/SDF-1α) as well as red fluorescence protein and BMP2 (pAd-RFP/BMP2) via biotin-streptavidin method,and the maximum efficiency of carrying DNA in microbubbles was detected.Three microbubbles with binary vectors were prepared by blending the two above-mentioned pAd at different ratio (1 ∶1,1 ∶ 2,2 ∶ 1) into the microbubbles.The microbubbles with binary vectors were evaluated though physiochemical properties,fluorescence microscope and flow cytometry to test the carrying rate of DNA in microbubbles.Results There was no significant difference in PH,average diameter and concentrations between targeted microbubbles and control group (P ＞0.05).The carrying efficiency of DNA increased with virus loads in microbubbles,but lowered if further increasing virus amount after reaching saturation.When the proportion of binary vectors and microbubbles was 1 ∶ 1,its efficiency of carrying SDF-1α gene and BMP-2 was approximately equal,and flow cytometry demonstrated that the positive rate of microbubbles labeled by both fluorescein isothiocyanate(FITC) and rhodamine was (65.6 ± 0.5)％.However,it was (59.0 ± 2.3)％ when their proportion was the 2 ∶ 1,which was significantly lower than those when other two proportions (1 ∶ 1 and 1 ∶ 2).Under the fluorescence microscope,the targeted microbubbles were equally surrounded by bright green or red fluorescence.Conclusions Ultrasound microbubbles of double genes carrying EGFP/SDF-1a and RFP/BMP2 is made successfully via biotin-streptavidin method.The optimal proportion of combining microbubbles with double gene is 1 ∶1,which can reveal the optimum load rate and stable combination.

Objective To observe the influence of comprehensive rehabilitation on lung function and exercise tolerance in geriatric patients with chronic obstructive pulmonary disease (COPD).Methods Totally 51 patients with COPD were enrolled in this study and divided into two groups:treatment group and control group.For treatment group,each patient was given health education about COPD and the rehabilitation program with medication therapy.The program included aerobic exercises,breathing exercises and upper limb strength training.The control group was given medication therapy only.The treatment group was tested by 6 minute walking distance (6MWT) and both groups had lung function examination before the program started,and were retested 1 year later.Results In the treatment group,lung function including vital capacity (VC),forced expiratory volume in one second (FEV1),FEV1 ％,and 6MWT were significantly improved after 1 year of treatment as compared with those indices before treatment [(2.94±0.67)L vs.(2.62±0.65) L,(2.88±0.70)L vs.(2.58±0.65)L,(1.89±0.79)L vs.(1.60±0.67)L,(80.7±28.3)％ vs.(67.7 ±25.9)％,(426.40±71.37)m vs.(326.23±110.80)m,t=2.86,2.27,4.28,4.73,-3.639,respectively,P=0.006,0.027,0.000,0.000,0.001].There was no significant difference in lung function before versus 1 year after intervention in the control group.After 1 year of intervention,the only difference was in FEV1 ％ between the treatment and control groups [(80.7±28.3)％ vs.(71.90± 16.5) ％,t=9.88,P=0.000].Conclusions Comprehensive rehabilitation can improve the lung function and exercise tolerance in geriatric patients with COPD.

Objective To get knowledge of the indoor air pollution states in the newly renovated apartments in Xi’an city, and explore the relationship among the major pollutants and the relationship between the pollutants and the differences of seasons. Methods From January, 2006 to May, 2007, we randomly selected 138 newly renovated (3 months or less) apartments in the urban area of Xi’an city, tested their indoor (including bedrooms, living room, dining room, kitchen, bathrooms, study, a total of 597) content of formaldehyde, total volatile organic compounds (TVOCs), ammonia, benzene, toluene and xylene. Results Among which were detected in all of the pollutants, total volatile organic compounds (TVOCs) and formaldehyde own the highest exceeding rates, respectively 69.8% and 60.8%. They are followed by ammonia, whose exceeding rate is 13.2%, xylene which has the highest exceeding rate among the benzene compounds, 8.7%. We did comparative analysis of the concentration in different types of rooms for the pollutants which are among the top four in exceeding rate ranks, and found that only for formaldehyde, there is significant difference in statistics (P

Objective To observe effects of the folic acid deficient diet on plasma homocysteine(Hcy) level and lesions of aorta in rats. Methods Twenty male Wistar rats were divided into two groups, folic acid deficent group (FD) and control group (Ctl).The rats were fed with folic acid deficient diet and normal diet for 3 months,respectively. The levels of serum folic acid and plasma Hcy as well as the activities of superoxide dismutase(SOD) and glutathione peroxidase (GPX) in erythrocytes were measured.The histological changes of aorta were also examined by light microscope. Results After 3 months treated with folic acid deficient diet, serum folic acid level 〔(6 08?1 84)?g/L〕 decreased significantly in rats of FD group compared either with pre experimental level 〔(13 32?2 02)?g/L〕 or with control one 〔(12 17?1 67)?g/L〕. Meanwhile, plasma homocycteine level increased significantly in rats of FD group 〔(28 66?6 07)?mol/L〕 compared either with pre experimental level 〔(9 75?1 86)?mol/L〕 or with control one 〔(9 49?1 77)?mol/L〕. The activity of erythrocytic SOD increased but GPX decreased obviously. The morphology lesions were also observed in aortic tissue. Conclusions Folic acid deficient diet induced hyperhomocysteinemia and arterial lesions.The high oxidant stress induced by hyper homocysteinemia may be one of mechanisms of arterial lesion.

Objective To explore the effects of Gantaikang on porcine serum-induced liver fibrosis in rats. Methods Hepatic fibrosis was induced in rats by intraperitoneal injection of 0.5ml porcine serum twice a week. Gantaikang was simultaneously administered intragastrically to rats in the intervention group at dose of 0.27g/kg?bw for 7 weeks. Then blood was collected, and serum levels of hyaluronic acid(HA), laminin(LN) and precollagen III(PCIII) were measured. Liver tissues was removed, and the pathologic changes of liver were observed by routine, Masson and James staining. The semi-quantitive analysis of collagen and reticulin in the liver tissues were performed. Results Gantaikang could effectively counteract the increase of HA, LN and PCIII level in serum, and attenuate hepatic collagen and reticulin proliferation induced by porcine serum in rats. Conclusion The results suggested that Gantaikang could, to a certain extent, prevent and attenuate immunologic hepatic fibrosis induced by porcine serum in rats.

Objective To evaluate the performance of bispectral index (BIS),auditory evoked potential index (AEPI) and cardiovascular response to electrical tetanus stimulus (ETS) for monitoring depth of sedation induced by propofol , analgesia produced by fenanyl and depth of isoflurane anesthesia. Methods Twenty ASA Ⅰ or Ⅱ patients of both sexes (11 males,9 females) aged 18-56 yr, weighing 50-80 kg undergoing elective brain surgery were studied. The patients were unpremedicated. The baseline BIS, AEPI, BP(SP, DP) and HR were measured before anesthesia. Propofol was infused at 0.25 mg?kg-1?min-1 and the infusion was stopped when the total amount of propofol infused reached 5 mg?kg-1 at the end of 20 min infusion. Fentanyl 2 ?g?kg-1 was then given i.v. Three minutes later the patients were intubated and mechanically ventilated. Tracheal intubation was facilitated by vecuronium 0.1 mg?kg-1. Anesthesia was maintained with isoflurane. Depth of sedation was measured using OAA/S scale during propofol infusion. ETS was given at the following time points: when the amount of propofol infused reached 2.5, 3.0, 3.5, 4.0, 4.5, 5.0 mg?kg-1,2 min after fentanyl administration and at 0.7, 1.0, 1.3 MAC isoflurane (end-tidal isoflurane concentration). BIS and AEPI values before and after ETS and changes in BP and HR (ABP, AHR) after ETS and the time for BP and HR to return to the pre-ETS value in 50% and 90% of the patients (TBP50%,TBP90%,THR50%,THR90% ) were recorded. Results BIS and AEPI were positively correlated with sedation depth. There were no significant changes in BIS and AEPI after ETS, but SP, DP and HR increased significantly after ETS( P