Physical diasnostic techenique is the core curriculum of clinical medicine speciality. It is the bridge curriculum between basic medicine and clinical medicine. To further improve the teaching level of clinical medicine speciality and to reform personnel training mode of clinical medicine speciality in higher vocational college, we investigate the relevant content according to the work process curriculum course developing route.

Objective Antiphospholipid antibody and factor Ⅻ deficiency are among the coagulation disorders that have been implicated in many thrombembolic events. The aim of this study was to investigate the prevalence of antiphospholipid antibodies and factor Ⅻ deficiency in patients with retinal vein occlusion (RVO). Methods The investigation was a case control study. The periphery blood was collected from a cohort of 33 consecutive patients with RVO and 30 age- and gender-matched normal subjects. Anticardiolipin antibody (ACA) was detected by ELISA as binding index (BI) =A value/standard A value. The lupus anticoagulant antibody was examined by APTT test and the activity of factor Ⅻ was detected. This study was approved by The Human Research Ethics Committee of this hospital, and written informed consent was obtained from all the subjects before initiation of any study protocol. Results The total positive rate of APA in RVO group was 24. 24% (8/33), showing a insignificant difference in comparison with control group (6. 67%, 2/30) (P = 0. 085). The positive rate of anticardiolipin antibody in RVO group was 18.18% (6/33), presenting an obvious enhance in control group (P = 0. 025) . Three patients in RVO group disclosed positive response for IgG-anticardiolipin antibody, one patient for IgM-anticardiolipin antibody, two patients for both isotypes IgG and IgM anticardiolipin antibodies, and two patients revealed positive reaction for lupus anticoagulant antibody. The presence of lupus anticoagulant antibody among the patients with ≤ 50 years and ＞ 50 years was similar to that in age-matched controls (P =0. 160, P =0. 206). Factor Ⅻ deficiency was found in 14 of 33 patients(42. 42%) and in 4 of 30 controls(13. 33%) (P = 0. 013). The prevalence of factor Ⅻ deficiency among the patients with ≤50 years and ＞ 50 years was similar to that in age-matched controls (P = 0. 206, P = 0. 052) . Conclusion Our results indicate that the prevalence of ACA and factor Ⅻ deficiency in RVO patients appears to be correlated.

Objective To investigate the adverse effect of chronic fluorosis on the testicular structure of rats testis and the antagonism of taurine-zinc. Methods 30 male Wistar rats were randomly divided into 5 groups (6 in each) and treated with fluoride and taurine-zinc through drinking water: control, low fluorine (LF, 100 mg NaF/L), high fluorine (HF, 200 mg NaF/L), low fluorine plus Zn(LF+Zn), high fluorine plus Zn (HF+Zn). After 5 months of fluoride treated, the rats in LF+Zn and HF+Zn groups were given taurine-zinc (0.34 g/L) by added in the drinking water for another month. Six months later, the testicle structure in all groups was examined under the microscope. Results In LF group, the loosened testicular gland and interstitial edema were seen. In HF group, primary spermatocyte became smaller and sperm head disappeared. However, in LF+Zn and HF+Zn groups there were no so significant damages, the changes were similar to normal testis. Conclusion Chronic fluorosis can cause testis damage and taurine-zinc presents a obvious antagonism in rats.

Objective To explore the influence of different lengths and contents of glass-fiber post on the fracture resistance of teeth, and to investigate the optimal length and content of glass-fiber post used for restorative treatment. Meth-ods Forty-two intact single canal mandibular premolars were collected from orthodontic patients and were divided into three experimental groups (A, B and C). All the teeth were endodontically treated after cut off the crowns from the 2 mm above cemento-enamel junction. The post lengths were10 mm in A group, 8 mm in B group, and 6 mm in C group.There were two subgroups for each experimental group (A1, A2, B1, B2, C1, C2 ), 7 teeth for each subgroup.The 42%glass fiber post was used in A1, B1 and C1 groups. The 75%glass fiber post was used in A2, B2 and C2 groups. All of groups were restored with resin cores and metal crowns. The fracture resistance was measured with universal testing machine and recorded the root frac-ture pressure data and the mode of all the teeth. Results The fracture resistance was significantly higher in group A than that of group B and group C (P<0.05), and the fracture resistance was significantly higher in group B than that of group C (P<0.05). The fracture resistance was significantly higher for 75%glass fiber post than that of 42%glass fiber post (P<0.05). Conclusion Within a certain range, the longer the length of glass fiber post, the greater the tooth fracture resistance. The fracture resistance of fiber content is better for 75%glass fiber post than that of 42%glass fiber post.

Objective To evaluate the clinical efficacy and safety of catheter ablation for persistent atrial fibrillation in patients with left ventricular dysfunction.Methods Thirty consecutive patients with symptomatic left ventricular dysfunction and left ventricular ejection fraction less than 45 percent underwent catheter ablation for persistent atrial fibrillation were enrolled.Sixty patients mathched according to age,sex,left atrial diameter and duration of atrial fibrillation but without left ventricular dysfunction were selected as the control.All patients underwent circumferential pulmonary vein isolation(CPVI) combined with ablation guided by complex fractionated atrial electrograms(CFAEs).Both parameters correlated with catheter ablation and complications in patients with left ventricular dysfunction were compared will the control.Left ventricular function and left atrial dimension at baseline and at 3 and 9 months after the ablation were evaluated by echocardiogram.Results The ablation procedurts were successful in all patients.The rate of pulmonary vein isolation,ablation time,X-ray exposure time and complications were not significantly different between the left ventricular dysfunction group and the control group(96.67% vs 98.33%,P=1.00;202.23?39.03 min vs 201.87?36.80 min,P=0.97;26.80?7.77 min vs 27.06?7.16 min,P=0.88;3.3% vs 3.4%,P=1.00).After a mean follow up of 11?1 months,73% of the patients with left ventricular dysfunction and 78% of the control remained in sinus rhythm(P=0.61).Forty percent of the left ventricular dysfurction group and 42% of the control received re-ablation respectively.Patients with left ventricular dysfunction showed significant improvement in left ventricular function(increase in ejection fraction by 7.87?4.72%,P

Objective To investigate the changes in heart rate(HR) after catheter ablation of atrial fibrillation(AF).Methods A total of 52 consecutive patients(mean age 64.65?10.18 years old) with symptomatic AF and no atrial tachycardia or AF recurrence during post-ablation follow-up were icluded in the study.Holter was performed to evaluate the changes in HR before the ablation,at one week and at one,three,six,and twelve months after the procedure.Results All the 52 patients received catheter ablation of AF successfully.The mean HR of all patients increased from 68.92?7.53 bpm at baseline to 75.58?6.48 bpm at one week after the procedure(P

As a special service,blood donation has special requirements for the ethics of blood donors,which is different from the professional ethics of ordinary employees or the social ethics of the general public.The present ethical status of blood donors calls for a new way of effectively cultivating the ethics of blood donors.

Objective To explore the total anti-oxidation capacity(T-AOC), superoxide dismutase(SOD), nitric oxide synthase(NOS) and nitric oxide(NO) in testis homogenate of the rats’ with chronic fluorosis induced by drinking NaF solutions with different concentration. Methods 24 male Wistar rats were divided into 3 groups (8 rats per group) including control group, low-fluoride(LF) group, high-fluoride(HF) group, which were exposed to fluoride by means of freely drinking tap water containing NaF with 0, 100 and 200 mg/L respectively for 20 weeks. T-AOC, NOS activities and SOD activity of the testis tissue were determined by colorimetry , xanthine oxidase method and nitrate reductase method respectively. Results Compared with the control group, the T-AOC of the testis tissue increased significantly in LF group and decreased significantly in HF group. SOD activity of the testis tissue showed on significant difference among the control group, LF group and HF group . Compared with the control group, the levels of NOS and NO decreased significantly in LF group and increased significantly in HF group. Conclusion The T-AOC of the testis tissue exposed to low level of fluoride presents a compensatory increase induced by the inhibited activity of NOS and synthesis of NO. Higher level of fluoride may increase reactive oxygen species(ROS), and cause abnormal expression of induce nitric oxide synthase(iNOS) and increased synthesis of NO in the testis tissue.

Objective To investigate the level of myocardical injury by measuring serum levels of cTnI, MYO, CK and CK-MB before and after catheter ablation in patients with atrial fibrillation. Methods Forty nine patients underwent catheter ablation for atrial fibrillation were involved in the study, cTnI, MYO, CK and CK-MB levels were measured before and 4 h after catheter ablation. Results Myocardical enzymes were increased in all patients (P0.05). Conclusion The serum myocardial enzymes levels increase after catheter ablation for atrial fibrillation. Of the above myocardical enzymes, cTnI is the most sensitive and specific parameters.

Objective To evaluate the effect of catheter ablation for atrial fibrillation on left ventricular function in patients with heart failure.Methods We retrospectedly studied 10 consecutive patients with congestive heart failure and a left ventricular ejection fraction of less than 45 percent who underwent catheter ablation for atrial fibrillation.The change in left ventricular function before and after catheter ablation was monitored during a short-term follow-up period.Results Nine patients underwent catheter ablation successfully.After a mean(SD) of 2.78?1.78 months,the patients showed improvement in left ventricular function through improvement in the left ventricular ejection fraction from 0.41?0.01 to 0.46?0.06,the left ventricular diastolic diameter decreased from 56.00?7.21 mm to 53.33?6.42 mm and the left atrial diameter decreased from 44.22?5.91mm to 42.11?4.65 mm,P