1.Infection Characteristics and Treatments on Diabetic Urinary Tract Infections
Herald of Medicine 2016;35(6):555-558
Diabetes complicated with urinary tract infection is influenced by many factors, such as low immune function, high blood and urine glucose environment prone for bacterial growth, and nervous lesion.Diabetic urinary tract infections are characterized by insidious onset, high recurrence rate, correlation with high blood glucose and difficulty to control.The related bacterial pathogens are mainly gram-negative bacillus genus ( 58. 59%-76. 19%) , in which the detection rates of extended spectrum β lactamases( ESBLs) Escherichia coli and Klebsiella pneumonia were the highest, followed by Enterobacter cloacae. Gram-positive bacteria only accounted for 23%. Drug-resistant bacteria and fungi would increase under the condition of repeated infection and irrational usage of antibiotics.In recent years, the urine-derived sepsis caused by urinary tract infection had become a popular clinical research topic because of its concealed symptoms, acute onset, rapid development and high mortality. By reviewing domestic and foreign literatures and expert consensus, this article is intended to explore the characteristics and treatment of diabetic urinary tract infections to supply reference for early prevention, correct diagnosis and effective treatments.
2.Clinical Studies on Female Genital Condyloma Accuminatum with Bupleurum Injection External Application
Journal of Zhejiang Chinese Medical University 2013;(11):1297-1299
[Objective] To observe the clinical effect of Bupleurum injection external application applied to female genital condyloma accuminatum cases. [Methods] From January 2010 to June 2013,we studied 60 female genital condyloma accuminatum cases in the outpatient department of our hospital, these cases were randomly divided into two groups:group A(Bupleurum injection group) and group B(ShuRun suppository, namely a-2a interferon suppository group), the clinical curative effect of the two groups was observed. [Results] After treatment, group A,30 cases in al ,9 cases had complete recovery,12 cases had some effect,9 cases were invalid, the total effective rate was 70%; group B,the total of 30 cases,6 cases had complete recovery, 7 cases had some effect, 17 cases were invalid, the total effective rate was 43.3%, group A in the treatment of female genital condyloma acuminatum had better effect than the group B(P<0.05).After treatment,the total score of two groups al reduced effectively.The recurrence rate had no obvious difference between the two groups(P>0.05). [Conclusion] Bupleurum injection for treating female genital condyloma acuminatum has better clinical efficacy, can be considered for clinical use.
3.CLONING OF ZNRD1 AND EFFECT OF ITS ANTISENSE VECTOR ON ACCUMULATION OF ADRIAMYCIN IN DRUG-RESISTANT GASTRIC CANCER CELLS
Yumei ZHANG ; Yanqiu ZHAO ; Xiaohan JIN
Medical Journal of Chinese People's Liberation Army 1981;0(04):-
The aim of this study was to construct antisense of ZNRD1 encoding gene, and to transfect it into SGC7901/VCR cells, to seek measures to overcome multidrug resistance of gastric cancer cells. ZNRD1 cDNA amplified by PCR was confirmed by DNA sequencing, and then inserted into the multiple cloning site of the expressing vector pcDNA3 1 + with molecular cloning technique. The recombinant vector was identified by endonuclease digestion. Antisense recombinant vector was transfected into SGC7901/VCR cells using lipofectamine. Flow cytometric analysis was used to detect adriamycin (ADM) accumulation in SGC7901/VCR cells. The results showed that a fragment was obtained by PCR, and its sequence was consistent with ZNRD1 cDNA reported in the literature. After antisense recombinant vector having been transfected into SGC7901/VCR cell, ADM concentration in such cells was increased. The above results indicated that the antisense vector ZNRD1 could enhance the intracellular accumulation of ADM in SGC7901/VCR cells, which might be of potential treatment value.
4.Characteristics and Treatment of Fever in Patients with Severe Craniocerebral Injury
Chencai LI ; Yumei DONG ; Guiming JIN
Herald of Medicine 2017;36(2):122-126
Fever in patients with severe craniocerebral injury was a frequent occurrence.The rate of fever patients lead to plant man or death high up to 28%-72%.The univariate analysis found that there was significant association between fever and motality.One of the common cause of fever was non-infectious fever,which related with craniocerebral injury,including central fever,dehydration fever,clonus and rebleading fever.The major complication was central high fever after servere craniocerebral injury.The temperature high up to 39 ℃,which aggravated the original basic diseases,lead to respiratory and circulatory failure.Another cause of fever was infection fever which associated with nosocomial infection.Serious disease,long hospiltal stay,high proportion of invasive operation,coma were the high risk factors of patients with craniocerebral injury.The major type of nosocomial infection were pudmonary infection and surgical site infection.By reviewing domestic and foreign literatures and expert consensus,this article was intended to explore the characteristics and mechanism of fever in patients with craniocerebral injury.In order to reduce the secondary brain injury,decrease the disability rate,increase the sutrvival rate,diagnosis and treatment should be taken early.
5.Pathogenic Bacteria of Abdominal Infection:A Clinical Analysis
Guiming JIN ; Xiujuan LI ; Yumei DONG
Chinese Journal of Nosocomiology 2009;0(24):-
OBJECTIVE To investigate pathogenic bacteria and prognosis of abdominal infection and take measures to prevent and control it.METHODS We analyzed the clinical material and prognosis of 31 patients with abdominal infection retrospectively according to the culture results of puncture and drain liquid from abdominal cavity from Nov 2007 to Nov 2008.RESULTS In 31 patients,5 were complicated with pulmonary infectionwhich the infection rate was 16.13%,and 7(22.58%) died.Totally 48 pathogenic strains were isolated from ascitic fluid culture,20 strains(41.67%) were Escherichia coli,11 strains(22.92%) were Enterococus.Some of coagulase-negative Staphylococcus,Streptococcus,Candida,Klebsiella pneumoniae,and Staphylococcus aureus were also isolated.The resistance rates of E.coli to cephalosporins were above 50.00%,to quinolones were above 65.00%.The susceptibility rates of E.coli to some ?-lactamases inhibitors and carbapenem were very high.The resistance rate of Enterococus to rifampicin was 63.64%,to piperacillin/tazobactam and piperacillin was 81.82%,to erythromycin was 100.00%.The susceptibility rates of Enterococus to teicoplanin,vancomycin,and linezolid were 100.00%.CONCLUSIONS The predominant pathogenic bacteria of abdominal infection are E.coli and Enterococcus.Abdominal infection tends to combine with pulmonary infection.The patients who got into multiple organ dysfunction syndrome have high mortality.
6.Sepsis-3-the use of sepsis in children:we still have a lot of work to do
Linmei JIN ; Ji QI ; Yumei LI
Chinese Pediatric Emergency Medicine 2017;24(7):486-490
Since the definition of sepsis has been first proposed in 1991, the main content of the definition has been developed by infection + systemic inflammatory response syndrome as infection + organ dysfunction,and organ dysfunction is indicated by an increase in sequential organ failure assessment(SOFA) score.These are components of Sepsis-3 which was published in 2016.In Sepsis-3,the SOFA score in the identification and diagnosis of sepsis plays an important role,but Sepsis-3 was mainly used in adults,how to adjust it to children,some questions and details were proposed in this article.
7.Occupation-safety education for prevention of sharp object injury among student nurses:a Meta-analysis
Liya JIN ; Yumei CHEN ; Duomei CHEN
Chinese Journal of Practical Nursing 2013;29(25):76-78
Objective To evaluate the effectiveness of occupation-safety education for prevention of sharp object injury among Chinese student nurses.Methods We searched PubMed,Springer,OVID,CNKI,Wanfang,and VIP databases for studies comparing the effect of education versus non-education for nursing students to prevent sharp object injury.Results 13 eligible studies were included in this metaanalysis.The pooled data revealed that sharp object injury was statistically significantly reduced in the education group.Furthermore,a significant more proportion of students in the education group dealt with the injury correctly.Conclusions Occupation-safety education is effective for student nurses to prevent sharp object injury.
8.Effects of Intermittent Catheterization Methods on Detecting Urine Bacteria in Patients with Spinal Cord Injury
Meihong ZHU ; Xudong GU ; Yumei JIN ; Meifang SHI ; Mei JIN
Chinese Journal of Nosocomiology 2009;0(20):-
OBJECTIVE To investigate the influence of intermittent catheterization methods and indwelling catheterization methods on the urinary tract infection in patients with spinal cord injury.METHODS After eighty cases of spinal cord injury,the urine retention patients caused by bladder dysfunction were respectively used intermittent catheterization and indwelling catheterization.The urinary tract infection rates of regucally urine culture were compared.By use of intermittent catheterization,the bladder function was trained.Bacteria culture and identification of the urine from the patients were conducted after 15 or 30 days of intermittent catheterization and indwelling catheterization respectively.RESULTS The rates of urinary tract infection(colony count≥1?10 CFU/ml) after 15 or 30 days of intermittent catheterization were 32.6% and 31.5%,respectively.Which were significantly lower than those of indwelling catheterization(100%)(P
9.Drug resistance and short-term efficacy of second-line drugs in patients with multidrug-resistant pulmonary tuberculosis in Zhejiang Province
Yumei FAN ; Fujian LI ; Qiang XIA ; Yan ZHANG ; Lijuan JIN
Chinese Journal of Clinical Infectious Diseases 2015;8(1):26-30
Objective To investigate drug resistance and clinical efficacy of second-line anti-tuberculosis drugs in patients with multidrug-resistant pulmonary tuberculosis.Methods A total of 183 multi-drug resistant pulmonary tuberculosis (MDR-PTB) patients received standard anti-tuberculosis treatment in Zhejiang Provincial Center for Diagnosis and Treatment of Tuberculosis during March 2011 and March 2013.Patients were divided into four groups according to the results of first-line anti-tuberculosis drugs susceptibility test:group A (n =30) resistant to isoniazid (H) and rifamipicin (R) ; group B (n =28) resistant to HR and ethambutol (E) ; group C (n =53) resistant to HR and streptomycin (S) ; groups D (n =72) resistant to HRES.Drug susceptibility tests of second-line drugs kanamycin (Km),protionamide (Pto),paraaminosalicylic acid (PAS) and levofloxacin (Lfx) were performed.Negative conversion rates of mycobacterium tuberculosis in sputum culture were also observed and compared among different groups with x2 test.Results Among 183 MDR-PTB patients,49 cases (26.8%) were resistant to Lfx,which was significantly higher than that of Km (8.7%,n =16),Pto (13.1%,n =24) and PAS (6.6%,n=12) (x2 =37.983,P<0.05).The resistant rate to Lfx in group D was 45.8% (33/72),which was higher than that in group A (2/30,6.7%),group B (6/28,21.4%) and group C (8/53,15.1%) (x2 =14.413,5.047 and 13.087,P <0.05).The occurrence of pre-extensively drug resistance (Pre-XDR) in group D was 34.7% (25/72),which was higher than that in group A (3/30,10.0%) and group C (9/53,17.0%) (x2 =6.499 and 4.852,P < 0.05).Among 157 MDR-PTB patients who received standard anti-tuberculosis treatment for one year,the negative conversion rate of mycobacterium tuberculosis in sputum culture was 87.3% (137/157).The negative conversion rate in group D was lower than that in other groups,but the difference was not of statistical significance (x2 =1.899,P > 0.05).Conclusions The efficacies of second-line anti-tuberculosis drugs vary among MDR-TB patients resistant to different firstline anti-tuberculosis drugs.The sensitivity tests results of the first-line drugs may serve as reference for MDR chemotherapy regimen in lack of test results of second-line drugs.
10.Effect of total nutrient admixture on lipid metabolism in acute hepatic failure rats
Yumei QI ; Ming ZHANG ; Yajun CHEN ; Jin XU ; Hui YANG
Chinese Journal of Clinical Nutrition 2009;17(1):41-44,后插1
Objective To study the effect of total nutrient admixture (TNA) on lipid metabolism in rats with acute hepatic failure (AHF). Methods Fifty-six male Wistar rats were randomly divided into four groups: normal diet group, nitrogen-free diet group, fat-free nutrient admixture group, and TNA group. All rats were injected with D-galac- tosamine to induce AHF model Meanwhile, 6 healthy Wistar rats were used as control group. TNA provided energy 221.75 kJ (1 kcal=4.184 kJ), nitrogen 0.365 g, nitrogen to energy ratio 1:145, amino acids 2.28 g (including branched-chain amino acid 0.70 g), glucose 7.85 g, and fat 1.25 g on a daily basis. After 10 days of the operation of Jugular vein puncture tube, blood glucose, blood lipid, liver and renal function were determined. Results The blood glucose level was significantly higher in TNA group than that in fat-free nutrient admixture group (P < 0.05). The levels of serum triglyceride and cholesterol were highest in fat-free nutrition admixture group, and triglyceride level was signif- icantly higher than that in normal diet group and nitrogen-free diet group (P <0.05). The levels of serum triglyceride and cholesterol were significantly higher in TNA group than those in normal diet group (P <0.05). The levels of ala- nine aminotransferase, alkaline phosphatase, total bilirubin and blood urea nitrogen were lower in TNA group than those in fat-free nutrient admixture group (P < 0.05). Conclusion The nutritional proportion of TNA is suitable for the metabolism disorder of hepatic failure, and therefore TNA can reasonably promote the anabolism and reverse the deteri- oration of hepatic failure in rats.