Through its high affinity with membrane receptors, growth factors promote tumor growth, metastasis and an-giogenesis, induce the change of tumor microenvironment and regulate immune response. It has been demonstrated that the serum protein levels of epidermal growth factor and its receptor, transforming growth factors and their receptors, insu-lin-like growth factors, and angiogenesis factors may have predictive value for metastasis and prognosis of breast cancer. Detecting serum levels of growth factors and their receptors which have synergistic effect or interaction with each other may be helpful for predicting the metastasis and progression of breast cancer.

Breast cancer is one of the most common cancers in women. Current clinical prognostic indicators and practice guide-lines show that almost 70% of node-negative and 30% of lymph node-positive breast cancer patients can survive 5-10 years without dis-tant metastasis after surgery. Patients also unnecessarily suffer the side effects of chemotherapy. Assays on multi-gene expression levels as prognosis prediction indicators have been successfully used to predict the prognosis of breast cancer patients because of the abnor-mal expression levels of a series of metastasis-related genes in cancer cells with high metastatic potential. In this review, we highlight the progress of research on predicting breast cancer prognosis based on multi-gene expression levels detected by microarray and qRT-PCR methods.

ObjectiveTo investigate effects of the arterial blood collection from radial artery,brachial artery,femoral artery and orsalis pedis artery for gas analysis of infants.Methods Three hundred and fifty two infants receiving blood collection were divided into four groups radial artery (n=92),brachial artery (n=94),femoral artery (n=73) and dorsal artery (n=53).Comparisons were done between them in terms of one-time success rate of puncturing and rate of complications from puncturing.Result The radial artery group had lowest rates of hematoma and mistaken puncturing into veins (P<0.05) and higher rate of one-time successful puncturing (P<0.001).Conclusion The radial artery is the first choice for infants undergoing arterial puncturing.

3 ?g/well.All BALB/c mice immunized with pJME by im.and gene gun injection,and JE inactivated vaccines by ip.injection survived for 21 days.BALB/c mice immunized with pJE by im.injection and gene gun injection partially survived for 21 days.Titres of neutralization antibody produced with pJME were higher than pJE.Protective immunity and titre of neutralization antibody produced by im injection was the same as gene gun injection(im/gene gun injection: 1∶320/1∶320) at day 21.The antibody from BALB/c mice sera after twice pJME immunization only reacts with JEV-E protein. Conclusions Expression efficacy of proteins encoded by pJME and pJE in transfected cells is different.Expression level of related proteins was dependant on recombinant amount for transfection in a certain degree.Immunity effect induced with pJME was higher than pJE.The efficacy of DNA immunization produced by gene gun injection was higher than im.injection.Titres of neutralization antibody induced by DNA immunization were correlated to efficacy of protective immunity.Neutralization antibody from BALB/c mice sera produced by pJME immunization contained anti-E antibody against JEV-E protein.

Objective To investigate the effects of intensive interaction on teaching of neurology for postgraduate for master. Methods The postgraduates of grade 2010 study under traditional teaching, and the postgraduates of grade 2012 study under intensive interaction teaching. Results The score of clinical knowledge test and specialized skill test were significantly better in the grade 2012 than in the grade 2010 (P<0.05). The students were satisfied with the intensive interaction teaching. Conclusion The intensive interaction teaching is good for teaching of neurology for postgraduate for master.

ObjectiveTo assess the efficacy of shoulder slings on rehabilitation of stroke patients with shoulder subluxation. Methods60 stroke patients with shoulder subluxation using shoulder sling or not were investigated retrospectively. ResultsAfter 3 months of rehabilitation training and nursing, both groups showed significant improvement in Fugl-Meyer Assessment of upper limbs and Barthel Index (P<0.05), but there is no significant difference between the two groups (P>0.05). ConclusionThere is no significant effect of should sling on upper limbs function and activity of daily living in stroke patients with shoulder subluxation.

Objective To investigate the genetic association between polymorphisms of Ser447Stop in lipoprotein lipase (LPL) gene and ischemic stroke. MethodsA case-control study was performed in 563 acute ischemic stroke patients and 320 controls. LPL Ser447Stop genotypes in the subjects were detected by polymerase chain reaction and restriction fragment length polymorphism assay. The frequencies of the alleles in each group were statistically analyzed with Student t-test. ResultsNo significant difference was found in the frequency of G allele of LPL Ser447Stop polymorphism between the case and the control. When subjects were divided into 2 subgroups of cerebral thrombosis infarction and lacunar infarction, the frequency of G allele was significantly higher in the cerebral thrombosis infarction group (9. 7％ ) than that in the control (6. 6％, χ2= 3. 99,P = 0. 045 ), and an increased risk for thrombosis infarction was suggested in the G allele ( OR = 1. 510, 95％ CI 1. 012--2. 261 ). ConclusionThe G allele in polyim Ser447Stop in LPL may be associated with increased risk of cerebral thrombosis infarction.

Objective To analyze the clinical characteristics of Candida parapsilosis septicemia of prematurity and provide a reference for both diagnosis and treatment.Methods The clinical data of 9 premature suffering with candida parapsilosis septicemia were retrospectively analyzed.Results All 9 cases (0.73 percent in the corresponding period) were premature infants with gestational age less than 37 weeks.The average weight of the patients was (1 621.00 ±372.22) g,among which 5 cases were of very low birth weight and 4 cases were of low birth weight.The average time of the fungi infection was (22.00 ± 7.58) d.Of the 9 cases,7 cases had been treated with broad spectrum antibiotics previously,9 cases were given nutrition intravenously,7 cases were treated with mechanical ventilation,7 cases adopted peripherally inserted central catheter(PICC) and 1 case received surgery.The symptoms included poor response,unstable serum oxygen saturation,jaundice,poor perfusion,abdominal distention,fever and no bodyweight increase.Laboratory tests results presented platelet decrease(6 cases),C-reaction protein increase (6 cases),high plasma (1-3)-β-D glucan level(9 cases) and white blood cells in the normal range(6 cases).All 9 infants recovered after receiving fluconazole and supporting treatment.Two cases failed to complete the hospital treatment but after discharge from hospital continued treatment by administering prescribed medicine,and through regular follow-up was done,the prognosis of all cases turned out to be good,and no case died.Conclusions Gestational age,birth weight,application of broad spectrum antibiotics,mechanical ventilation and peripherally inserted central catheter are common high risk factors of Candida parapsilosis septicemia in premature infants.The disease was featured with normal white blood cell,low platelet,high serum levels of C-reaction protein and (1-3)-β-D glucan.Anti-fungi therapy with fluconazole may be a good choice for it.

Objective To observe the effect of gene gun transduction of K-RAS Antisense gene on the expression of K-RAS P21 protein in human pancreatic carcinoma cells.Methods K-RAS Antisense gene was transduced into pancreatic carcinoma cells by gene gun transduction, the expression of K-RAS P21 protein on BxPC-3、AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells line were examined by western blot and immunocytochemistry staining. Results The expression of K-RAS P21 protein in AsPC-1 and MiaPaCa-2 pancreatic carcinoma cells was obviously lower after the transduction of specific K-RAS Antisense gene,which has little impact on BxPC-3 pancreatic carcinoma cells. Conclusion Gene gun transduction of K-RAS Antisense gene is a potential method for treatment of pancreatic carcinoma.

Objective To investigate the significance of haman epididymis protein 4(HE4)as a tumor marker combined with CA125 in diagnosis of epithelial ovarian cancer.Methods Serum levels of HE4 and CA125 were examined by ELISA and electrochemiluminescence method in 31 patients with ovarian cancer,68 patients with benign pelvic masses(36 benign ovarian tumor,23 ovarian chocolate cyst and 9 pelvic abscess were included) and 40 normal women as controls.Results Serum levels of HE4 and CA125 in ovarian carcinoma group were significantly higher than those in the benign pelvic masses group and healthy women group(P<0.01), and the difference of these levels between benign pelvic masses and normal ones have no statistical meaning(P>0.05).The sensitivity and the specificity of HFA were 74.2% and 95.0% respectively,of CA125,were 80.6% and 87.5%.respectively,and if the two markers combined,were 93.5% and 82.5%.Conclusions HFA is an ideal tumor marker and it has certain values in accessory diagnosis and differential diagnosis of ovarian cancer.The clinical value call be improved by combined detection of HE4 and CA125.