Objective:To statistics and analyze the detection rate of 5 kinds of common respiratory virus specific IgM in children with respiratory tract infection to guide the clinical.Methods: 1 609 cases of children with respiratory tract infection in department of Pediatrics in our hospital were collected in 2012 from 1 to 12 months.Serum was collected to detect the specific IgM of respiratory syncytial virus (RSV),adenovirus (ADV),influenza A virus (IVA),influenza B virus (IVB) and parainfluenza virus ( PIV) by method of indirect immunofluorescence.Results:263 positive cases were detected from 1 609 cases ( 16.35%) , and the positive rates of IVB was the highest (8.45%) with a total of 136 cases,followed by the rest of IVA 63 cases(3.92%),PIV 62 cases (3.85%),ADV 44 cases(2.73%) and RSV 42 cases(2.61%).The positive detection rate was different in children of different age groups,and the difference was statistical significant (χ2=134.822,P<0.01).Positive detection rate was gradually increased along with the age growth,and the detection rate of school-age children was the highest (34.55%);The comparison differences of IVB ,PIV,IVA positive detection rate between different age stages were significant (χ2=102.660 ,86.145 ,39.791 ,P<0.05 ).The detection rate of IVB and IVA was the highest in school-age children,20.00%and 8.64%respectively.The detection rate of PIV was the highest (12.07%) in preschool children.The positive rates of different respiratory virus specific IgM had their own characteristics in different months .IVB appeared two positive peaks in March and October with the rate of 20.15%and 11.11%respectively;IVA appeared two positive peaks in March and May with the rate of 10.27% and 11.81% respectively;PIV and RSV , the detection rate of which was the highest ( 7.98%and 6.46 respectively ) in March increased gently from January to June ,and then declined steadily.The detection rate of ADV was the highest (6.19%) during three months of March ,April and May.Conclusion:The positive rate of 5 kinds of respiratory virus specific IgM was 16.35%,especially the detection rate of IVB was the highest.Positive detection rate of respiratory virus specific IgM was gradually increased along with the age growth;The detection rate of IVB and IVA was the highest in school-age children ,while PIV in preschool children.5 kinds of respiratory tract virus own respectively prevalent characteristics in 2012.

OBJECTIVETo analyze the association of micoRNA-related genes DROSHA single nucleotide polymorphisms (SNP) rs10719 and rs6877842, DICER1 rs3742330and GEMIN4 rs3744741 with prognosis of T-cell lymphoma.

METHODSPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genotypes of the above 4SNPs and their associations with complete remission (CR) rate and overall survival (OS) in 163 patients with TCL.

RESULTSPatients carrying the rs6877842 CG genotype had a significantly higher CR rate compared with those carrying the CC genotype (OR=0.07, 95% CI 0.01-0.72, P=0.026); the same for patients carrying the DICER1 rs3742330 GG genotype compared with those carrying the GA genotype (OR=0.15, 95% CI 0.02-0.97, P=0.047) or the AA genotype (OR=0.11, 95% CI 0.02-0.71, P=0.020). In addition, patients with the DICER1 rs3742330 GG genotype had a significantly improved OS compared with those carrying the GA (HR=9.02, 95% CI 1.22-66.92, P=0.031) or AA genotype (HR=8.77, 95% CI 1.19-64.67, P=0.033). The other two SNPs of rs10719 and rs3744741 had no significant association with CR or OS.

CONCLUSIONDROSHA rs6877842 and DICER1 rs3742330 were independent factors for TCL CR, and DICER1 rs3742330 was also an independent prognostic factor for TCL OS.

DEAD-box RNA Helicases ; genetics ; Genetic Predisposition to Disease ; Genotype ; Humans ; Lymphoma, T-Cell ; diagnosis ; genetics ; MicroRNAs ; genetics ; Minor Histocompatibility Antigens ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Prognosis ; Ribonuclease III ; genetics ; Ribonucleoproteins, Small Nuclear ; genetics