BACKGROUND:The establishment of animal models of Parkinson’s disease plays an important role in clinical and basic experiments of Parkinson’s disease, and its stability directly affected study results. OBJECTIVE:To evaluate the behavior and pathological changes of rats with Parkinson’s disease established by unilateral two-point injection with 6-hydroxydopamine.
METHODS:A total of 62 Sprague-Dawley rats were randomly assigned into experimental group (n=50) and normal group (n=12). Using stereotatic technique, 6-hydroxydopamine was injected into the right substantia nigra
RESULTS AND CONCLUSION:At 2 weeks, after induction with apomorphine, left rotation speed>7 r/min was observed in only 22 rats of the experimental group. No significant difference in the number of rotation between 2 and 4 weeks was detected (P>0.05). Tyrosine hydroxylase and dopamine content significantly reduced in the model group. Results indicated that unilateral two-point injection of 6-hydroxydopamine could be used to
successful y establish rat models of Parkinson’s disease with similar behaviors and pathologies.

Objective To analyse the mental health status of doctors in the First Affiliated Hospital of Xinjiang Medical University. Methods The 673 doctors in different clinic character in the First Affiliated Hospital of Xinjiang Medical University were investigated by questionnaire and symptom checklist-90(SCL-90),and estimate the results through F-test and t-test . Results Doctors in SCL-90 in addition to interpersonal relationships(1.59±0.49, t=-1.02,P>0.05) and the rest of the factors:somatization (1.48 ± 0.55, t=2.32), force(1.75 ± 0.43, t=2.34),depress(1.67 ± 0.44,t=3.00), anxiety(1.55 ± 0.57, t=3,71), hostility(1.76±0.47,t=5.65),horrible(1.48±0.32,t=6.35),stubborn(1.65±0.39, t=4.04),insanity(1.43±0.46, t=3.39)are higher than the national norm, there is statistical difference (P<0.05). In different gender groups, in addition to the somatization and forced, female doctors other project factors were higher than male doctors, including interpersonal relationships(1.62 ± 0.37, 1.56 ± 0.41), hostility factor(1.79 ± 0.38, 1.73 ± 0.43) differences were statistically significant (t=2.15, t=2.07, P均<0.05). In different professional title ,Doctors with primary professional title in addition to the body of the rest are higher than the title doctor. Anxiety factor differences have statistical significance in different groups (F=2.73, P<0.05). In different department , Emergency department and pediatric clinicians in addition to depression, anxiety and other factors of points were higher than those of physicians and surgeons, the difference in interpersonal relationship factors have statistical significance in different groups (F=4.54, P<0.05). Conclusion The scores aboutdoctors, SCL-90 in the First Affiliated Hospital of Xinjiang Medical University in addition to interpersonal relationships, the rest of the factors are higher than the national norm ,showing that clinic doctors were in psy chological sub health state, especially in female doctors, primary professional title dortors and Emergency department and pediatric clinicians.

Objective:To investigate the influence of IL-2 on the apoptosis of activated and inactivated lymphocytes.Methods:Selected an appropriate system as the model of activated lymphocytes by detecting the expression of Fas and FasL in two systems of one way MLR using RT-PCR. Detected the apoptosis of activated and inactivated lymphocytes after the stimulation of IL-2 with the methods of FITC-Annexin-V/PI staining.The resultaint change of membrane Fas was evaluated by FACS,and the lymphocytes from doner and acceptor were distinguished by CFSE staining.Results:To the inactivated spleen lymphocytes, exogenous IL-2 inhibited their apoptosis and the expression of Fas; while to the spleen lymphocytes activated by mixed lymphocyte reaction, exogenous IL-2 promoted their apoptosis and upregulated the expression of Fas, which did the same both to the doner cells and the acceptor cells.Conclusion:IL-2 can inhibit the apoptosis of inactivated lymphocytes, and promote the apoptosis of activated lymphocytes.These effects have something to do with the change of expression of Fas.

Objective To investigate the tumor-inhibitory effect of cytokine-induced killer cells(CIK)co-cul-tured with dendritic cells (DC)pulsed by breast cancer stem cell antigen on the same tumor-bearing mice. Methods Breast cancer stem cells were isolated from the cell line of MCF-7/ADR and extract lyses antigen of the stem cell was saved. DC and CIK derived from peripheral blood mononuclear cells of healthy individuals were co-cultured and pulsed or un-pulsed by the above antigen lyses. This DC+CIK were injected to breast tumorbearing mice (BCSC-AP-DC+CIK group), and were used to compared with the common breast cancer cell antigen (rather than breast cancer stem cell antigen) pulsed DC+CIK group(AP-DC+CIK group), DC+CIK group, CIK CIK group and normal saline group(NS group). The tumor-inhibitory effect were evaluated and compared among all 5 groups through the tumor size, TdT-mediated dUTP nick end labeling test (TUNEL), examining expression level of bcl-2 and bax by immunohistochemistry. Results The tumor size in each group before and after therapy and the tumor size after therapy between each group was of significant difference(P＜0.05). The maximum size is NS group(3.625±0.093)cm3 and BCSC-AP-DC+CIK group is minimum,which is (1.234±0.131)cm3. BC-SC-AP-DC+CIK group is of highest expression of bax and apoptotic index value, lowest bcl-2 expression in all 5 groups. Conclusion The CIK co-cultured with DC pulsed breast cancer stem cell antigen was more effective to induce apoptosis of breast cancer cells than those of CIK cells co-cultured with DC pulsed breast cancer cell antigen,CIK cells co-cultured with DC and CIK cells.

Objective To observe differences between repeat and written response in speech recognition tests of short Mandarin monosyllable lists. Methods 12 normal-hearing subjects were tested by 6 short monosyllabic lists. The subjects should repeat and write down.the contents of the tests at the same time, the tester monitors au-rally and records the subjects' reactions. The speech recognition scoring error under different responses at different presentation levels could be obtained by comparing the records of the subjects with the tester. Results The speech recognition scoring of repeat and written response were highly correlated with each other (P<0.01). However, 19(26.4%) out of 72 pairs of test records were inconsistent. The error range of them was from -5% to 10%. When the presentation level was 5 dB HL, the difference among the speech recognition scoring was significant statistically, but not significant when the presentation level at 20, 15, 10 and 0 dB HL. Conclusion The test results of speech audiometry are impacted by different respond methods. In the open-set test, it's supposed that the subjects should make notes while repeating the contents of the tests, then compare with the aural monitoring records of the tester to keep the reliability of the test.

Objective To analyze the efficacy and prognosis of different antiviral agents on compensated hepatitis B cirrhosis.Methods Eight hundred and sixty-five cases of Hepatitis B patients with compensated cirrhosis were randomly divided into five groups:A group(n =143,subcutaneous injection interferon 3 million U every other day for 48 weeks),B group(n =171,orally treatment with lamivudine 100 mg/d for 48weeks),C group(n =137,orally treatment with adefovir 10 mg/d for 48 weeks),D group(n =83,oral treatment with entecavir O.5 mg/d,for 48 weeks) and E group(n =331,treated with general hepatoprotective drugs).Clinical symptoms and signs,changes in liver function were observed and prothrombin time activity degrees (PTA),quantitative HBV-DNA,HBeAg seroconversion and HBeAg/HBeAb conversion situation were detected.Results There were significant differences on HBeAg seroconversion between A group and other four groups after treated for48 weeks(31.5％ vs 13.5％,22.6％,18.1％,5.7％,x2 =58.56,P ＜0.01) and at the end of follow up (33.1％ vs 14.2％,23.3 ％,23.6％,6.4％,x2 =52.87,P ＜ 0.01).Patients' condition in A,B,C,D groups progressed slowly,and their incidence rates of decompensate cirrhosis were significantly lower than that in group E(x2 =122.41,P ＜ 0.01).The incidence rate of liver cancer in group A was significantly lower than that in B,C,D,E groups (x2 =19.61,P ＜ 0.01).Conclusion Antiviral therapy of interferon and nucleoside drugs can slow the progression of compensated Hepatitis B cirrhosis and reduce the incidence rates of decompensate cirrhosis.And interferon can significantly reduce the incidence rate of liver cancer.

Objective:To investigate the antioxidant activity of the total flavonoids of Cinnamomum septentrionale Hand.-Mzt. Methods:A ferricyanide reduction method was used to study the reduction ability. The scavenging activity of the total flavonoids on DPPH· free radical( DPPH·),hydroxyl radicals( ·OH)and superoxide anion free radical( O2-·)was investigated as well. Re-sults:Cinnamomum septentrionale Hand.-Mzt. had good reduction and scavenging activity on the free radicals in a concentration-de-pendant manner. When the concentration of total flavonoids of Cinnamomum septentrionale Hand.-Mzt reached 0. 15 mg·ml-1 ,86%of DPPH free radical( DPPH·),68% of hydroxyl radicals( ·OH)and 59% of ultra oxygen anion free radical( O2-·)were scav-enged. Conclusion:Cinnamomum septentrionale Hand.-Mzt. has good antioxidant activity,and as a natural antioxidant,it is worthy of further research and development.

Objective To evaluate Mandarin disyllables recognition scores in noise for normal hearing people , and to establish a model for teaohing .The second goal is to get the spatial separated advantage while the noise chan‐ging its direction .Methods Percentage of correct word recognition was measured for each list by testing 50 Manda‐rin-speaking people aged from 18 to 30 with normal aural/oral communicational abilities And .6 of them joined the pilot study aimed to identify a presentation level that would be used in the formal test .The other 44 subjects partici‐pated in the formal speech test .Results When the noise was at 0 and 90 ,the speech recognition changed along with the change of signal-to-noise ratio levels .Despite of the speech recognition effect ,there was a strong relation be‐tween the signal-to-noise ratio of 0° and 90° .Conclusion The direction of speech and noise may strongly influence the speech recognition scores .When the noise and signal is separated ,the score will be better .

Objective To establish a method for quality control of xinshuaining mixture. Methods The herbal contents including red ginseng,milk veteh,root of red rooted salvia were identified by thin layer chromatography ( TLC) and the content of ginsenoside Rb1 in xinshuaining mixture was determined by high performance liquid chromatography ( HPLC ) . Results The herbs can be overtly identified by TLC. Ginsenoside Rb1 had a linear relationship in the range of 1. 095-5. 475μg, and the average recovery was 97. 3%(RSD 1. 98%). Conclusion The method is simple and rapid,and can be used for the quality control of xinshuaining mixture.

Estrogen Receptor (ERalpha) is a member of superfamily of ligand-activated transcription factors which play critical roles in many biological processes. To screen novel modulators of ERalpha for drug development and biological function research, we developed a mammalian one-hybrid-based high-throughput screening model for ERalpha modulator. We cloned the ERalpha LBD gene from the total mRNA of fat tissue by RT-PCR and fused it with the GAL4 DNA binding domain of pBIND-GAL4 plasmid to construct a chimara expression plasmid pBIND-GAL4-Eralpha(LBD). The L02 cells was cotransfected with pBIND-GAL4-ERalpha(LBD) and a GAL4-responsive luciferase reporter plasmid pGL3-GAL4, and following treatment with test compounds for 24 h, the activities of luciferase were detected to evaluate the transactivities of ERalpha modulators. After manner optimizations of transfection conditions, Estradiol, an agonist control, induced the expression of luciferase in a dose-dependent with EC50 of 0.17 micromol/L, the maximum folds of induction was about 28.1. Tamoxifen, an antagonist control, efficiently suppressed the estradiol-mediated luciferase induction with EC50 of 0.10 micromol/L. Using this screening model, we discovered four ERalpha agonists from 2000 natural and synthetic compounds.
3T3-L1 Cells ; Animals ; Chimera ; metabolism ; DNA-Binding Proteins ; biosynthesis ; genetics ; Estrogen Receptor Modulators ; chemistry ; isolation & purification ; Estrogen Receptor alpha ; agonists ; Genes, Reporter ; genetics ; Genistein ; chemistry ; isolation & purification ; HeLa Cells ; Humans ; Luciferases ; genetics ; metabolism ; Mice ; Models, Chemical ; Saccharomyces cerevisiae Proteins ; biosynthesis ; genetics ; Transcription Factors ; biosynthesis ; genetics ; Transfection