Objective To analyze the correlation between 5-hydroxytryptamine transporter gene linked polymorphic region(5-HTTLPR) and twin children's behavioral problems and its interaction with environmental factors.Methods A total of 147 pairs of school-age twins aged 6-12 years were enrolled.The behavioral problems were assessed with the Child Behavior Checklist (CBCL).The genotype of 5-HTTLPR was detected by the method of PCR.The correlation of 5-HTTLPR and children's behavioral problems was performed using generalized estimating equation.Results (1) The overall incidence rate of behavioral problems in school-age twins was 24.15％ (27.94％ in the boys;20.89％ in the girls),in which the rate of thinking problems (15.31％) was the highest,and the rates of other problems were lower (3.40％-8.16％).(2) The results of correlation analysis showed that 5-HTTLPR was only related to the social problems (P＜ 0.05).The S allele of 5-HTTLPR maybe associated with the social problems.(3) The results of interaction of 5-HTTLPR with twin factors of themselves and family environmental factors showed that 5-HTTLPR was the main effect of the anxious/depressed problems,and S allele carriers were easier to occur anxious/depressed problems.Gender was the main effect of the social problems,and boys were easier to occur social problems.There was no significant interaction between 5-HTTLPR and twin factors of themselves and family environmental factors.Conclusion 5-HTTLPR genetype may have some relevance to the social and anxious/depressed problems,and S allele may be a susceptibility gene of social and anxious/depressed problems.

Objective To investigate the in vivo effect of silenced actin-associated protein Transgelin on the growth of human pancreatic carcinoma xenograft in nude mice.Methods Human pancreatic cancer cell line BxPC3 were transfected with small hairpin RNA (shRNA) eukaryotic expression vector targeting Transgelin gene.RT-PCR and Western blot were used to analyze Transgelin expression after transfection.24 animal models were randomly divided into three groups with 8 in each:Experimental group (transplanted BxPC3/Transgelin shRNA),negative control group (transplanted BxPC3/Neo) and untreated group (transplanted BxPC3).Tumor size was measured weekly.All mice were sacrificed after 28 days.Tumor volume was calculated,inhibitory effect was analyzed.Immunohistochemical staining of paraffin sections for Transgelin and proliferating cell nuclear antigen (PCNA) proteins were performed.Results Tumors varied in sizes among 3 groups (all P ＜ 0.05).On day 21 and 28 tumor was significantly smaller in experimental group than those in control groups.Tumor weighed(0.74 ±0.21) g in experimental group,lower than that in negative control group(1.42 ± 0.28) g and untreated group(1.59 ± 0.24) g (all P ＜ 0.05).The inhibitory effect was 53.5％ in experimental group.The PCNA index was significantly lower in experimental group than those in control groups (all P ＜ 0.05).Conclusions Deletion of Transgelin gene can significantly inhibit the proliferation and tumor growth of BxPC3 cells in nude mice.

Objective To explore characteristics and treatment of the clinical crises myasthenia sufferer.Methods Retrospective analysis 32 patients with clinical data of MG crises,intravenous immunoglobulin all.Results Treating group,recovered in 1 case,the basic recovery in 4 cases,markedly effective in 6 cases,improved in 3 cases,Ineffective in 2 cases;the total effective 31.25%.Conclusion Group,recovered in 0 case,the basic recovery in 1 cases,markedly effective in 2 cases,improved in 3 cases,Ineffective in 10 cases; efficient 37.50%.Two groups of efficient comparative greup(X2 = 4.54 ,P <0.05) conclusion:Improved the patients's clinical symptoms after intravenous injection of gamma globulin,and the titer of patients with blood AchRAb significantly lower than that before treatment.

Objective To explore the application effect of individualized traditional Chinese medicine nursing mode in the nursing of patients with subtotal gastrectomy. Methods A total of 60 eligible patients who met the standard were divided into the control group and the intervention group with 30 cases in each group according to the random number table method, the control group received only normal nursing while the intervention group was subjected to the individualized TCM nursing based on the normal nursing. The nursing effect was compared between two groups. Results Scores of negative mood of patients of the intervention group before surgery, electrogastrogram slow wave 3 h, the first day and the second day after the operation, first feeding time after operation, early ambulation time, average length of stay was (16.50 ± 3.20) points, 2.58 ± 0.46, 2.88 ± 0.08, 2.99 ± 0.54, (28.70 ± 4.43) h, (29.09 ± 4.55) h, (8.24 ± 1.38) d. The control group was(16.98 ± 2.61)points, 2.40 ± 0.53, 2.79 ± 0.14, 2.71 ± 0.30,(31.45 ± 3.80)h,(32.91±2.28)h,(10.40±1.99)d, there were significant differences between the two groups (t=2.53-25.19, P < 0.05). In the third day after operation, the electrogastrogram slow wave in the intervention group and the control group were 2.94 ± 0.53, 2.93 ± 0.12, there was no significant difference between the two groups (t=0.40, P>0.05). Conclusions Individualized TCM nursing can effectively alleviate the patients′ negative mood, shorten the recovery time of postoperative electrogastrogram slow wave, first feeding time and early ambulation time, to restore the gastrointestinal function of patients earlier, effectively promote the rehabilitation of patients;at the same time, shorten the hospitalization time.

OBJECTIVE:To establish a method for the content determination of saccharide in polysaccharides containing galact-uronic acid based on phenol-sulfuric acid method combined with correction factor method. METHODS:The determination condi-tion of phenol-sulfuric acid was optimized. A series of standard curves were drawn with glacturonic aid-glucose mixed control with different mass ratio. The content of saccharide in Codonopsis pilosula polysaccharides CPP1b samples containing galacturonic acid was determined. According to regression equation of galacturonic acid-glucose ratio of 0-100%,the correction factor was calculated by using C. pilosula polysaccharides CPP1b as the reference polysaccharide,and the results of content determination of saccharide were corrected. The rationality of this method was verified by using mixed monosaccharide control with same composition as C. pi-losula polysaccharides CPP1b. RESULTS:The correction factor of C. pilosula polysaccharide CPP1b to glucose was 3.33;in vali-dation test,the content of saccharide in mixed monosaccharide control with same composition as C. pilosula polysaccharides CPP1b was 103.47%. RSDs of precision and stability tests was <1%. The recoveries ranged 93.52%-107.35%(RSD=5.09%,n=6). CONCLUSIONS:The established method can accurately determine the content of saccharide in C. pilosula polysaccharides CPP1b containing galacturonic acid.

Objective: To uncover the underlying mechanisms of action of the Yinlai decoction on high-calorie diet-induced pneumonia through proteomics analysis. Methods: Based on the Gene Expression Omnibus (GEO) database, lung tissue samples from normal and high-fat diet (HFD) fed mice in the GSE16377 dataset were selected as test cohorts to identify differentially expressed genes and conduct bioinformatics analyses. In the animal experiments, mice were randomly divided into the control (N), high-calorie diet pneumonia (M), and Yinlai decoction treatment (Y) groups. Mice in the M group received high-calorie feed and a 0.5 mg/mL lipopolysaccharide solution spray for 30 min for 3 d. The mice in the Y group were intragastrically administered 2 mL/10 g Yinlai decoction twice daily for 3 d. Pathological evaluation of the lung tissue was performed. Differentially expressed proteins (DEPs) in the lung tissue were identified using quantitative proteomics and bioinformatics analyses. The drug-target relationships between Yinlai decoction and core DEPs in the lung tissue were verified using AutoDock Vina and Molecular Graphics Laboratory (MGL) Tools. DEPs were verified by western blot. Results: GEO data mining showed that an HFD altered oxidative phosphorylation in mouse lung tissue. The Yinlai decoction alleviated pathological damage to lung tissue and pneumonia in mice that were fed a high-calorie diet. A total of 47 DEPs were identified between the Y and M groups. Enrichment analysis revealed their association with energy metabolism pathways such as the tricarboxylic acid cycle (TCA) and oxidative phosphorylation. The protein-protein interaction network revealed that Atp5a1, Pdha1, and Sdha were the target proteins mediating the therapeutic effects of Yinlai decoction. Molecular docking results suggested that the mechanism of the therapeutic effect of Yinlai decoction involves the binding of brassinolide, praeruptorin B, chrysoeriol, and other components in Yinlai decoction to Atp5a1. Conclusion The Yinlai decoction alleviated lung tissue damage and pneumonia in mice that were fed a high-calorie diet by regulating the TCA and oxidative phosphorylation. Our study highlights the importance of a healthy diet for patients with pneumonia and provides a scientific basis for the prevention and treatment of pneumonia through dietary adjustments.

Objective:To explore the effect of reduction, readiness, response and recovery (4R) crisis management concept in sepsis nursing.Methods:From March 2018 to January 2021, 140 patients with sepsis admitted to the First Affiliated Hospital of Zhengzhou University were selected by convenience sampling. The patients were divided into the control group (69 cases) and the observation group (71 cases) according to the hospital odds and even numbers. The control group adopted the traditional nursing of sepsis, and the observation group adopted the 4R crisis management concept. The condition, the incidence of complications of the two groups of patients were compared.Results:After nursing, the Quick Sequential Organ Failure Assessment (qSOFA) scores of the two groups were lower than those before nursing, and the qSOFA score of the observation group was lower than that of the control group, with statistical differences ( P<0.01) . The incidence of complications in the observation group was lower than that in the control group, and the difference was statistically significant ( P<0.05) . Conclusions:The application of the 4R crisis management concept in sepsis nursing has a good effect, which can effectively relieve the patient's condition, reduce complications.

Objective? To explore the application effect of six-step standard communication process in nursing communication with elderly patients with lung cancer. Methods? A six-step standard communication process training was conducted for 38 nurses in the Thoracic Surgery Department of the First Affiliated Hospital of Zhengzhou University from January to June 2017 selected by purposive sampling. Nurses＇ Clinical Communication Competence Scale (NCCCS) was used to evaluate the changes of nurses＇ communication ability with patients before and after training. A total of 38 elderly patients with newly diagnosed lung cancer in Thoracic Surgery Department from January to December 2016 were enrolled as the control group, and during their in-hospital period of time, the nurses had not received the training and their communication was in the conventional way; a total of 35 elderly patients with newly diagnosed lung cancer in Thoracic Surgery Department from July 2017 to June 2018 were enrolled as the observation group, and during their in-hospital period of time, the nurses had already completed the training and their communication mode was based on the six-step standard communication process. The two groups were compared in terms of their scores in Profile of Mood States-Short Form(POMS) 24 hours after getting newly diagnosed (before intervention), 24 hours before operation(after intervention), and their nursing satisfaction degree upon discharge. Results? After training, nurses＇ communication ability in difficult situations, emotional perception ability, emotional support ability, basic language communication ability and basic non-verbal communication ability were significantly improved with statistical differences (P＜ 0.05). There was no statistical difference in each dimensional scores in POMS between the two groups before intervention(P＞0.05);after intervention, the observation group had lower scores in dimensions of"depression-dejection", "tension-anxiety","anger-hostility", "confusion-bewilderment", and "fatigue-inertia" than the control group with statistical differences(P＜ 0.05). The observation group had higher nursing satisfaction degree upon discharge than the control group with statistical differences(P＜0.05). Conclusions? For elderly patients with lung cancer in Thoracic Surgery Department, the application of six-step standard communication process can effectively improve the nursing communication efficacy,improve patient＇s bad mood and improve nursing satisfaction.

Objective:To explore the application value of chronic disease trajectory-based nursing in the management of COPD patients outside the hospital.Methods:From January 2018 to December 2019, convenience sampling was used to select 226 discharged COPD patients from the First Affiliated Hospital of Zhengzhou University. The patients were divided into the control group and the research group with the random number table method, each with 113 cases. The control group carried out routine out-of-hospital health management, and the research group carried out out-of-hospital chronic disease trajectory model-based nursing, and the interventions all lasted for 3 months. The Exercise of Self-care Agency Scale (ESCA) was used to evaluate the self-management ability of the two groups, and the Dyspnea, Degree of Airflow Obstruction, Smoking Status, the number of Exacerbation (DOSE) index was used to evaluate the disease control effect of the two groups.Results:After intervention, the ESCA score of the research group was (114.45±3.26) , which was higher than (99.92±6.56) of the control group, and the difference was statistically significant ( t=20.666, P<0.001) . After intervention, the DOSE index of the research group was (2.28±0.08) , which was lower than (2.72±0.09) of the control group, and the difference was statistically significant ( t=34.692, P<0.001) . Conclusions:The chronic disease trajectory model-based nursing can improve the self-management ability of COPD patients outside the hospital and control their condition.

Objective To evaluate intraoperative placement of bile duct decompression drainage tube in CBD primary suture after laparoscopic choledocholithotomy.Methods 152 patients undergoing laparoscopic choledocholithotomy were divided into group A (placement of bile duct decompression drainage tube during operation,82 cases) and group B (no decompression drainage tube placement,70 casas).Results There was no significant difference in operative time and intraoperative bleeding between the two groups (P ＞ 0.05).In group B patients the abdominal drainage tube indwelling time,abdominal drainage volume,peritoneal drainage fluid bilirubin value,postoperative hospitalization time were significantly longer than group A,the difference was statistically significant (P ＜ 0.05).No recurrence of choledocholithiasis and biliary stricture were found in either groups.Conclusions Placement of bile duct decompression drainage tube in laparoscopic choledocholithotomy and CBD primary suture after laparoscopic choledochotomy can significantly reduce the incidence of biliary leakage and shorten the postoperative hospital stay.