Objective To explore the changes of serum IL‐6 ,IL‐10 ,PLA2 on intracranial infection and its prognosis .Methods Totallly 100 patients with intracranial infection during February 2011 to December 2013 were selected as the research object .In 100 cases of intracranial infection patients ,30 cases were cured (group A) and 52 cases improved (group B) ,18 cases of illness or death as poor prognosis group (group C) .Serum IL‐6 ,IL‐10 ,PLA2 content of the subjects in different time point were detected .Results One day After infection ,serum IL‐6 ,IL‐10 ,PLA2 levels in intracranial infection group were obviously higher than that of healthy control group (P< 0 .01) ;7 d after infection ,IL‐6 ,IL‐10 average level among 3 groups had obvious changed(P< 0 .05) ,7 d after in‐fection ,IL‐6 ,IL‐10 level of group B and group C was significantly higher than group A (P< 0 .05) ;and in group C ,IL‐6 ,IL‐10 was higher than group B (P< 0 .05) .7 d after infection ,the IL‐6 IL‐ 10 level of group A declined ,the IL‐6 ,IL‐ 10 levels of group B be‐gan decreasing 14 d after infection ,and the IL‐6 ,IL‐10 levels of group C had been in a rising trend .3 d after infection ,the PLA2 leves among 3 groups had obvious changed(P< 0 .05) ,7 d after infection ,the change rate increased ,7 d after infection ,PLA2 level of group B and group C was significantly higher than group A (P< 0 .05) ,and in group C ,PLA2 was higher than group B (P< 0 . 05) .7 d after infection ,the PLA2 level of group A declined ,in group B and group C ,PLA2 level began to decline significantly 14 d after infection .Conclusion IL‐6 ,IL‐10 ,PLA2 are closely related to the occurrence and prognosis of intracranial infection .

Objective To study expression of hypoxia inducible factor 1? (HIF 1?) in the lungs of hypoxic rats and to explore the role of HIF 1? in the pathogenesis of hypoxic pulmonary hypertension Methods Twenty Wistar rats were randomly divided into control group and hypoxia group The models of hypoxic pulmonary hypertension were established by exposure to hypoxia for 4 weeks according to our laboratory protocol Digoxin labelled cRNA probe for HIF 1? was prepared by in vitro transcription Northern blot was performed by using the HIF 1? cRNA probe and In situ hybridization was also conducted with rat lung tissue sections Results Northern blot hybridization showed minimally positive in the lung tissue of control group ,but strongly positive in hypoxia group In situ hybridization analysis with hypoxic rat lung tissue revealed that HIF 1? mRNA was expressed in bronchial epithelial cells (strongly positive reaction) and peribronchial proliferative lymphomatic tissue (weakly positive reaction),casually in alveolar wall cells (weakly positive reaction),but not in pulmonary arterial endothelium and smooth muscle cells Conclusions Our data suggested that chronic hypoxia could induce pulmonary hypertension characterized by pulmonary vascular remodeling HIF 1? mRNA expression was elevated in the lungs of rat under hypoxic condition HIF 1 may be involved in the pathophysiological changes in response to hypoxia and play an important role in the development of hypoxic pulmonary hypertension

ObjectiveTo study the anti-proliferative effect and the mechanism of arotinoid Ro40-8757 on human pancreatic carcinoma cell line JF-305.MethodsMTT assay was used to evaluate the growth inhibition effect of Ro40-8757 on human pancreatic carcinoma cell line JF-305. The expression of p27 protein was measured by Western blotting, and flow cytometry for cell cycle analysis.ResultsRo40-8757 significantly inhibited the growth of JF-305 cells with a dose-, time-dependent manner. After 72 hours treatment with 10 -5mol/L Ro40-8757, the proportion of cells in G 1 phase increased from 56% to 76% while the cells in S phase decreased from 25% to 7%. Western blot analysis showed that the expression of p27 protein in cells treated with Ro40-8757 increased markedly.Conclusion Ro40-8757 inhibits the growth of pancreatic carcinoma cell line JF-305 possibly by up-regulating p27 protein expression and arresting cell cycle.

Objective To discuss the surgical techniques and efficacy of a new method of breast treatment of hypermastia. Methods From January 2009 to February 2011, a series of 15 patients underwent a new technique for reduction mammaplasty. This technique involved a superomedial pedicle nipple-areolar complex (NAC). Results In our series, the resection weight per breast was (522. 9±218.3) g and the new nipple-to-sternal-notch distance was (21.7± 1.74) cm postoperatively. Mean nipple elevation was 8. 5cm (range, 4-16 cm). There were no hematoma and partial or complete NAC necroses occurred in our series. Minimal wound dehiscence occurred in one case ( 6.7 % ). Thirteen patients were followed up for 6 to 18 months. NAC sensibilities were comparable before and after operation, and good breast shapes were produced in all cases. Conclusion The superomedial pedicle technique provides good results with respect to NAC viability and sensibility, breast contour and last superomedial fullness.

Infantile spasm is the most common epileptic encephalopathy in infancy.Without early diagnosis and effective treatment, serious consequences and economic burden maybe caused.One of the main characteristics of infantile spasm is the hypsarrhythmia on electroencephalography (EEG). It is important to analyze EEG accurately and reliably by scoring EEG.In order to further improve the EEG scoring system and better play the role of EEG in the diagnosis, treatment and prognosis implications of infantile spasm, in this paper, the advantages and disadvantages of various EEG scoring systems were analyzed, so as to provide clues by summarizing current EEG scoring systems and their applications in clinical practices.

Objective:To evaluate and compare the identification of several DNA barcoding candidate sequences on Illicium difengpi and its fake I. jiadifengpi. Method:Samples from different origins of I. difengpi and I. jiadifengpi, were collect extraction of total DNA,nuclear gene ITS2 sequence,chloroplast rbcL,matK gene sequence were selected for PCR amplification,product purification and sequencing,and CondonCode Aligner V3.7.1 was used to proofread stitching. Result:PCR amplification and sequencing of rbcL sequences of I. difengpi and I. jiadifengpi were not satisfactory. It is assumed that their rbcL sequences were too long with slow evolution,which was unsuitable for interbreeding. The success rate of matK sequencing of I. difengpi and I. jiadifengpi was 0 and 76.8%,which may be because primer standards were different for matK sequences of different groups. The results of PCR amplification and sequencing of ITS2 on I. difengpi and I. jiadifengpi were successful,with the success rate of sequencing was 89.3% and 91.2%. Analysis sequencing results, the total length of ITS2 sequences was 268 bases,and there were 2 variation sites of I. difengpi. The total length of ITS2 sequences was 430 bases,and there were 4 or 3 variation sites of I. jiadifengpi. It shows that ITS2 sequences of I. difengpi and I. jiadifengpi were short and has obvious variability and can be amplify,that ITS2 sequence was better than rbcL and matK sequence in molecular identification of I. difengpi and I. jiadifengpi. Conclusion:DNA barcoding based on ITS2 sequence was a powerful and efficient tool for identification of I. difengpi and its fake I. jiadifengpi.

【Objective】 To investigate the practice and benefit of national standardized management of type 2 diabetes in Yulin City. 【Methods】 We recruited the adult type 2 diabetes patients who sought medical help at our hospital from May 2020 to October 2022 as subjects. We collected their basic information (sex and age); measured height, weight, waist and hip circumference, and blood pressure; calculated body mass index (BMI); and detected blood glucose, c-peptide, HbA1c, biomarkers, urinary microalbumin, sensory nerve conduction velocity of lower limbs, ABI, and subcutaneous and visceral fat at the time of MMC recruited and the end of six months. T test and Mann-Whitney U rank sum test were used for measurement data and χ² test or Fisher’s exact probability method for counting data to analyze the data. 【Results】 After 6 months, the levels of fasting blood glucose, postprandial blood glucose, HbA1c, and visceral and subcutaneous fat in all the patients decreased, but the level of fasting c-peptide increased compared with the baseline (all P<0.05). Secondly, compared with the baseline, the control rate of HbA1c (35.21% vs. 13.71% ) and the comprehensive control rate (13.97% vs. 7.26% ) were both significantly increased at six months (P<0.05). Thirdly, after 6 months, the levels of fasting blood glucose, postprandial blood glucose, HbA1c, TG, TC, and UA were decreased more, while the fasting c-peptide and postprandial c-peptide were increased more in the patients of the HbA1c standard group (HbA1c<7% ) than those of the non-standard group. 【Conclusion】 The multiple benefits of blood glucose, blood lipid, uric acid and islet function can be achieved by taking type 2 diabetes patients into MMC. Meanwhile, the rates of HbA1c control and comprehensively reaching the standard are significantly increased. Therefore, MMC can explore a new way for the management of type 2 diabetic patients in this area.

Objective To investigate the therapeutic effect of intravascular embolization of carotid-cavernous fistulation.Methods 5 cases were examined by DSA of brain to find out the sites of fistulation.The detachable balloon or GDC(Guglielmi Detachable Coil)were employed in embolotherapy.Results 1 cases were embolized successful with detachable balloon and kept free circulation of ICASs;The embolism of ICA involved side were performed in 2 cases for patient with pseudoaneurysm and 1 patients cavernous involved were embolized with GDC for small mouth of the fistula.Another case balloon raptured two times when it was detached to cavernous after one free balloon was dept in cavernous,the patient didn't want to embolized the ICA involved,the trentment was stoped,the patients symptoms was disappeated after one week of therapy.Conclusion The site and size of carotic-cavernous fistulation can be detected by DSA of brain intravascular embolization is the first-choise method of therapy for it.

Objective To assess the quality of randomized controlled trials (RCTs) of delirium prevention of elderly published in China.Methods The literatures from China National Knowledge Infrastructure(CNKI),WANFANG Data and VIP Database for Chinese Technical Periodicals were evaluated according to Cochrane collaboration's tool for assessing risk of bias and Jadad scale.Results A total of 53 RCTs were included,14 (26.4％,14/53) described radom number table used to generate the random allocation sequence,4 (7.5％,4/53) conducted experiments in a blinded manner,7 (13.2％,7/53) did not use intentionto-treat to analyse those who did not complete the study,9 (17.0％,9/53) had high risk of other bias,none described allocation concealment mechanism and blinding to participants and intercention implementers.Based on Jadad scales,the score was 1-4,average score was (2.3±0.8),19 (35.8％,19/53) were high-quality literatures.Conclusions The quality of present published literatures is not high,the further domestic studies should be designed high-quality to better improve clinical practice.

Objective To investigate the effect of atorvastatin on the expression and activity of matrix metalloproteinases (MMPs) in rabbit abdominal arterial atherosclerotic plaque. Methods Eighteen male New Zealand rabbits weighing 2 kg were randomized to normal control group (n=6) and hypercholesterolemia group (n=12). The latter was given hypercholesterol diet for 2 weeks, and then catheter-induced abdominal aortic wall injury was performed. Rabbits in hypercholesterolemia and aortic injury group were randomized to model group (n=6,4 weeks of hypercholesterol diet) and atorvastatin(5 mg?kg-1?d-1 for 4 weeks)group (n=6). Finally, the levels of MMP2 and MMP9 protein and mRNA in the abdominal aortic artery were measured by immunohistochemical analysis, reverse transcription-polymerase chain reaction (RT-PCR), sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) zymography. Results The intimal-medial thickness[(0. 49?0. 072) mm vs (0.66?0.079) mm, P