Mesenchymal stem cells (MSCs) are multipotent stem cells existing in multi-organs and can differentiate into several kinds of somatic cells. Recently it has been found that MSCs, under defined conditions in vivo or in vitro, can be induced to trans-differentiate into islet-like cells, which can secrete a small amount of insulin and lower blood glucose levels in diabetic mice. Thus, two major obstacles of islet transplantation, the insufficience of donor islets and immunologic rejection, might be solved simultaneously with this technique. This finding provides a new insight into treatment for diabetes.

Pancreatic cancer is one of the most lethal human malignant tumors, and its incidence is rising. Now, its treatment includes surgical resection,chemo - radiotherapy and supplementary treatment. The prognosis of pan-creatic cancer is still dismal. And its one-year survival is low. This article talks about the effect of clinical treatment on quality of life in patients with pancreatic cancer.

Objective　To investigate the biological significance of p185 and p16 in breast cancer. Methods　Immunohistochemical S-P method was used to detect the expression of p185 and p16 proteins in 59 breast carcinomas，and their relations to clinicopathologic features were analyzed. Results　The positive rates of p185 and p16 were 47% and 34% respectively. No significant association was evident between the two proteins. There was also no significant relation between p16 expression and clinicopathologic features. p185 positive rate was 64% in the group of lymph-node involvement that was higher than that being 32% lymph-node negative one (P<0.05). Conclusion　p185 and p16 may play important roles independently in the development of breast cancer through different pathways. p185 detection is of important value in breast cancer.

Objective To assess the efficacy and safety of paroxetine for epileptic patients following depression. Methods 156 epileptic patients were recruited. Patient Health Questionnaire 9-item Depression scale (PHQ-9) then the Hamilton Scale for Depression (HAMD)were the assessment tools. 31 patients, whose score of PHQ-9 more than 15 and HAMD more than 20 were selected. The monthly seizure frequency and depression severity were investigated before and 12 weeks after treatment with paroxetine, 20 mg/d. The side effects of paroxetine were also observed. Results 29 patients completed the observation. The total scores of the HAMD decreased 12 weeks after treatment (P<0.05). Dizziness was the most common adverse event during the first month of treatment. No seizure worsening was observed. Monthly seizure frequency did not change significantly. Conclusion Paroxetine is a safe and effective antidepressant for epileptic patients following depression.

Objective To study the effects of active and passive movement of the affected hand after ischemic stroke on brain activity patterns using blood oxygenation level-dependent functional magnetic resonance imaging (BOLD-fMRI) and to explore the central mechanism of movement treatment for hand disability.Methods Five pa-tients with subcortical cerebral infarction in Brunnstrom stages 1 to 3 (both upper limb and hand affected) were investigated using BOLD-fMRI during active and passive clenching and relaxing of the affected hand.Statistical parametric mapping software (SPM5) was used to integrate the activity data and display them in one standard brain map.The activated areas were then compared.Results The BOLD-fMRI signals aroused by both active and passive move-ment were enhanced in the contralateral sensorimotor cortex,the contralateral premotor cortex,bilaterally in the sup-plementary motor area and in the bilateral cerebellum.Both movements also activated the ipsilateral sensorimotor cor-tex and premotor cortex,which are not normally activated during such movements in healthy people.The areas were more extensive and the activation was stronger during passive movement.Moreover,the activated brain areas induced by active movement were mainly on the contralateral side,while passive movement induced activation distributed over both hemispheres almost evenly.Conclusions Both active and passive movement significantly activate the brain areas responsible for movement of the affected hand.Both are useful for boosting brain reorganization after stroke.

A GC method is described for measuring fentanyl in man plasma. Fentanyl was extracted at basic pH with cyclohexane-isobutyl alcohol (197:3) with Ro21-2212 added as internal standard and fentanyl derivative as earner. The drug was back-extracted in H2SO4, then the extract was made basic and recxtracted with ethyl ether-dichloromethane (9:1). The organic phase was evaporated at 40℃ under N2 and the residue was dissolved in ethanol. A. portion (2?l) was analyzed on a wide-bore capillary column (10 m ? 0.53 mm) of HP-1 (2.65 ?m), operated at 255℃ with N2 as carrier gas (8 ml/min) and N -P detector. The calibration graph was linear within the ranges of 0.25-100 ng/ml with a corelation coefficient of 0.9996 and the detected limit was 0.2 ng/ml. Mean recovery was 99.02% ? 6.81%. The coefficient of variation for the within- and between-run were all less than 8%. No interference was found from endogenous compounds, metabolites of parent drug or other commonly used drugs. The method was applied to therapeutic drug monitoring and pharmacokinetic studies.

Objectives:To investigate the differentiation of human hepatocellular carcinoma cell line Bel-7404 induced by Matrine in vitro,and discuss its mechanism.Methods:After dealing human hepatocellular carcinoma cell line Bel-7404 with different concentrations of Matrine,we drew cell growth curve by MTT colorimetry,and cell morphology was examined by light microscope.Then we evaluated the tumor cell biological characteristics changes through improved soft agarose clone forming test,cell adhesion assay,cell movement assay and cell invasion assay.Also we detemined cell cycle distributing by flow cytometry.Results:After 72 hours being treated with 0.5,0.75,1.0g/L,in the Matrine test groups,the cell proliferation was inhibited significantly,and cell shape transformed to normal.The amounts of clone were remarkably reduced.The cell adhesion ability increased in different level.At the same time cell ability of movement and invasion differently weakened.Analysis of cell cycle shown that along with rising of Matrine concentration,the cell amounts in G1 phase increased accordingly and cell amounts in G2 and S phase remarkbly decreased.Conclusion:During the appropriate concentration range,Matrine not only inhibited Bel-7404 growth and proliferation,but also induced tumor cell to differentiate.Its probably mechanism may related to cell cycle regulation.

Aim To study the effect of 5F on tubulin polymerization.Methed 5F was added into the tubulin polymerization-depolymerization system and the changes of Absorption values at 350 nm, 37 ℃ were recorded. Results 5F decreased the Absorption values of the reaction system.and markedly inhibited not only tubulin polymerization but also tubulin depolymerization. Conclusion 5F inhibits tubulin polymerization and depolymerization, and leads to cell cycle arrested, and possibly induces apoptosis.

Objective: To explore heart rate variability (HRV) and arrhythmia in patients with resistant hypertension.
Methods: Our work included 3 groups:Resistant hypertension (RH) group, n=39, Non-resistant hypertension (NRH) group, the hypertension could be controlled by 1-3 medications, n=42 and Control group, composed of 40 healthy subjects. The 24h ambulatory blood pressure and electrocardiogram were monitored, the time-domain of HRV and arrhythmia parameters were compared among 3 groups which including standard deviation of all normal RR intervals (SDNN), standard deviation of the average normal RR intervals for all 5-min segments (SDANN), the mean of standard deviation of all 5-minute RR intervals (SDNN Index), the root mean square of the differences between adjacent R-R intervals (rMSSD) and the percentage of adjacent R-R intervals varied by more than 50 ms (pNN50).
Results: Compared with Control group, both RH and NRH groups showed decreased time-domain HRV parameters, all P<0.05. Compared with NRH group, RH group had the lower time-domain HRV parameters, all P<0. 05, while the rMSSD was similar between 2 groups (t=-1.5048, P=0.1364). The rates of arrhythmia in both RH and NRH groups were higher than Control group. The incidences of premature atrial contraction and atrial tachycardia were higher in RH group than NRH group, P<0.05, while the premature ventricular contraction, ventricular tachycardia and A-V block were similar between 2 groups, all P>0.05.
Conclusion: RH patients had decreased HRV and increased arrhythmia, especially with the higher incidence of atrial arrhythmia.

ABSTRACT]OBJECTIVETo study the compliance to sublingual immunotherapy(SLIT) in patients with allergic rhinitis(AR) in Guangzhou city.METHODSFrom January 2014 to May 2014, 202 patients with AR received SLIT were followed up by telephone. According to age, the patients were divided into group A(age<14 years) and group B(age≥14 years). The compliance to SLIT was analyzed and the reasons of poor compliance were investigated.RESULTSAmong 202 patients, only 93 cases(46.04%) were successfully followed up by telephone, 109 cases(53.96%) were lost to visit. Among the 93 cases of successful follow-up, the good compliance rate was 29.03%(n=27), the poor compliance rate was 70.97%(n=66). compliance to SLIT was not affected by age and gender(P>0.05). Main reasons for poor compliance included poor efficacy (48.48%), insufficient education about SLIT (16.67%), inconvenience (15.15%), and adverse reactions(10.61%).CONCLUSION In Guangzhou city, lost follow-up rate in AR patients receiving SLIT is high. Compliance to SLIT is relatively low and improvements shall be made.