Objective To explore the effect of the health education with teaching -back method on senior citizens' hypertension related health literacy in nursing homes. Methods Six nursing homes, randomly selected using stratified cluster random sampling method, were randomly divided into the experiment group and the control group,151 cases in each group. The control group received peer education method, and the experiment group received the health education with teaching-back method on the basis of the control group. The hypertension related health literacy total scores and 4 dimension scores of the two groups were compared before and after the intervention. Results There was no significant difference in the total score and each dimension score of the hypertension related health literacy between the experiment group and the control one before intervention (P>0.05). There was significant difference in the total score and each dimension score of the hypertension related health literacy between the experiment group and the control one after intervention (P<0.001). The total score and each dimension score of the hypertension related health literacy of the experiment group were significantly higher than that of the control group. There was significant difference in the total score and each dimension score of the experiment group before and after intervention (P<0.001). The scores of the experiment group after intervention were significantly higher than before. There was significant difference in the total score, knowledge attainment score, faith accomplishment score and technical accomplishment score of the control group before and after intervention (P < 0.01). The score of the control group after intervention were significantly higher than before. But the behavior literacy before and after intervention was not statistically significant (P>0.05). Conclusion The health education with teaching-back method can improve the elderly hypertension related health literacy level and improve the quality of health education.

The study of the relationship between trace elements and drug resistance of tumor has been going on for a long time.It is clear that some trace elements are related to the tumor occurrence and its progress, such as selenium,zinc,copper.So they can be used as an sub index to diagnosis and to evaluate prognosis. Further study in recent years has shown that trace elements are related to the tumor drug risistance. Improveing nourishment of tumor cells may change its drug resistance and influence the curative effect of tumor. The significance of this study is to search for a simple and approach in reducing drug resistance in order to improve the chemotherapeutic effect and the patient′s prognosis.

Objective To discuss training of the nurses in outpatient blood collection room with the digital training modular of blood sample collection to improve the quality of blood sample collection. Methods Nurses were trained with the digital training modular by multimedia,group discussion to impmve the quality of blood sample collection continuously. Results The unqualified blood sample rate in the same season after training were statistically different compared with that before training. Conclu-sions Training the nurses in outpatient blood collection room with the digital traimg modular of blood sample collection have actual direction value to improve the quality of blood sample collection.

OBJECTIVETo screen the allergens in patients with allergic rhinitis (AR) in Yibin, Zhaotong and some areas of the Changjiang River, and to study the distribution characteristics of inhaled allergens. METHODSThe clinical data of 661 patients with allergic rhinitis who diagnosed by skin prick test from March 2013 to June 2015 in the First people's Hospital of Yibin City were studied.RESULTSIn 661 patients, the top 5 allergens and their positive rates of skin prick test were Dermatophagoides farinae (76.5%), house dust mites (72.01%), Roach (35.85%), cat hair (11.35%) and dog hair (8.62%) respectively. There were no statistical difference of the positive rates of allergens among different seasons (χ2=4.568, 0.206). With the increasing age of the patients, the positive rate of allergens was decreased. CONCLUSION The dominated allergens in patients with AR are dust mites, house dust mites and cockroaches in Yibin, Zhaotong and and some areas of the Changjiang River. The prevention and control of AR in children should be toke more attention.

Objective To describe the general characteristics of patients with acute chest pain in order to analyze factors associated with patients’utilization of emergency medical services (EMS).Methods A total of 747 eligible patients with acute chest pain admitted to emergency department of Qilu Hospital were consecutively enrolled from October 2014 to April 2015.Clinical data including demographic features, mode of arrival,past medical history,risk factors,symptoms and signs were collected prospectively by using standardized case report form.Univariate and multivariate analyses were carried out to investigate the association between the decision to use EMS and related factors including demographic features,past medical history,risk factors,symptoms and signs.Results Of the total 747 eligible patients,414 (55.4%)were male ,and the mean age was (57.2 ± 15.8)year;333 (44.6%)were female,and the mean age was (61.7 ±14.9)year.Of them,171 (22.9%)patients used EMS,and 143 chest pain patients with more than 75 years old were more inclined to use EMS (P <0.01),whereas 152 patients in 65 -75 years age group accounted for the lowest proportion of using EMS.Men were more inclined to use EMS than women (P <0.05),and 483 patients with typical chest pain used more EMS than patients with atypical chest pain (P <0.05);Of them,356 patients with a history of hypertension and 54 patients with a history of cerebral infarction were more inclined to use EMS (P <0.05 and P <0.01,respectively).Multivariate logistic regression analysis showed that male,older than 75 years,history of cerebral infarction were independent factors associated with EMS use (P <0.05).Conclusions This study indicated that only less than one-third of emergency department visits with acute chest pain decide to use EMS when symptoms occurred. Factors including male,older than 75 years,and a history of cerebral infarction were associated with more use of EMS.In order to promote patient asking for EMS timely,more work should be done.

Objective To observe the effects of aminoguanidine and Vitamin C on lipid of streptozotocin-induced didbetic rats.Methods Diabetic nephropathy rats were induced by intraperitoneal injection of streptozotocin.Fifty Sprague-Dawley(SD) rats were randomly divided into normal control group,model group,Vitamin C group,aminoguanidine group and Vitamin C associated with aminoguanidine group.All the rats were treated for 16 weeks.During and after the treatment,the general state of the rats was observed and the levels of blood sugar,triglyceride,total cholesterol,low density lipoprotein,high density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein in different groups were detected.Results The rats in the four groups suffered diabetes mellitus and renal function lesion.Aminoguanidine and Vitamin C could improve the general state of the rats,but had no effect on the blood sugar.Vitamin C could decrease the levels of triglyceride,serum cholesterol,low density lipoprotein,glycosylated hemoglobin,and glycated low density lipoprotein,and increase the level of high density lipoprotein.There was a synergistic effect with the combination of the two drugs. Conclusion Aminoguanidine and Vitamin C have no effect in the decrease of blood sugar level but have a certain effect on regulating blood lipid.There is a synergistic effect with the combination of the two drugs.

Objective To investigate the effect of Morroniside which was isolated from Cornus officinalis on the growth and the protection against H2O2-induced injury in SH-SY5Y neuroblastoma cells.Methods SH-SY5Y cells were incubated with Morroniside in different concentration.MTT metabolic rate was measured as the cell survival rate in order to determine the best effective concentration to promote the survival of neuron.MTT metabolic rate,axonal length,area of cell body and cell counting were used as the indicators to analyze the effects of Morroniside on the growth of SH-SY5Y on the best effective concentration.After SH-SY5Y cells were injured with 500 μmol/L H2O2,they were administrated with Morroniside in different concentration and vitamin E.The expression of neural growth factor(NGF)were determined with Western Blotting.Results Morroniside increased the cell survival rate in 10～100 μmol/L.Compared with control group,MTT metabolic rate(P<0.05),axonal length(P<0.001),area of cell body(P<0.01),cell counting(P<0.05)increased in morroniside-treated group after SH-SY5Y cells were incubated with Morroniside in 10 μmol/L.The expression of NGF in morroniside-treated group was higher than that of the control group after injury induced by H2O2.Conclusion Morroniside shows the neurotrophic effect and can protect SH-SY5Y cells from injury induced by H2O2.

OBJECTIVE: To investigate the antioxidant effect of different solvent extracts from persimmon leaves (PL) in diabetic mice induced by streptozotocin (STZ). METHODS: The total ethanol-extracted fraction of PL was further extracted with chloroform, ethyl acetate and n-butanol, in that order, the residues after ethanol extraction were water-extracted and alcohol-precipitated, and concentrated. The hypoglycemic effects of different solvents extracts from PL were evaluated in diabetic mice induced by STZ. The experimental mice were randomly divided into groups: control group, model group, glibenclamide group, low and high dosage groups of the various solvent extracts. The drugs were administrated to mice in every morning for 15 days. During this time period, the contents of malondialdehyde (MDA) and superoxide dismutase (SOD) were determined. RESULTS: The water-extracted and ethanol-precipitated fractions and the ethyl acetate-extracted fraction markedly reduced the content of MDA and increased the activity of SOD in the livers of STZ-induced diabetic mice (P<0.01 or P<0.05). The chloroform-extracted and n-butanol-extracted fraction did not markedly reduce the content of MDA nor increase the activity of SOD in liver of STZ-induced diabetic mice (P>0.05). CONCLUSION The ethyl acetate-extracted fraction, water-extracted and ethanol-precipitated fraction of persimmon leaves have potential value in the treatment of diabetes. The mechanism of action of the antioxidant is related to the hypoglycemic effects of extracts from persimmon leaves.
Animals ; Antioxidants ; metabolism ; Diabetes Mellitus, Experimental ; drug therapy ; metabolism ; Diospyros ; chemistry ; Female ; Hypoglycemic Agents ; therapeutic use ; Male ; Mice ; Plant Extracts ; therapeutic use ; Plant Leaves ; chemistry

OBJECTIVEThe purpose of this study was to investigate the regulatory role of tyrosine kinase 2 with immunoglobulin-like and epidermal growth factor homology domains (Tie2) on apoptosis and proliferation in the endothelial cells.

METHODSRNA interference (RNAi) technique was used to silence Tie2 gene expression by transfecting an expression vector containing short hairpin RNA(shRNA) for Tie2 into human umbilical vein endothelial cells (HUVECs). Real time quantitation reverse transcriptase polymerase chain reaction (QRT-PCR) and Western blot were used to monitor Tie2 mRNA, as well as protein expression. The proliferation of HUVECs was examined by methyl thiazolyl tetrazolium (MTT), and the apoptosis was detected under microscope. HUVECs transfected with pGenesil-hk was negative control, and HUVECs transfected with nothing was empty control.

RESULTSTie2 mRNA expression was down-regulated 24 h and 48 h after transfection, and Tie2 protein expression was significantly down-regulated at 24 h and 48 h (P< 0.05), especially 48 h after transfection. The apoptosis rate was conspicuously higher in experimental group than in negative control and empty control group after 48 h (P<0.05). The growth monitoring showed that proliferation was also markedly inhibited in experimental group (P<0.05) compared with two control groups.

CONCLUSIONDown-regulated expression of Tie2 by RNAi can promotes apoptosis of HUVECs and has an anti-proliferation activity effect on them.

Apoptosis ; Cell Line, Tumor ; Cell Proliferation ; Down-Regulation ; EGF Family of Proteins ; Human Umbilical Vein Endothelial Cells ; Humans ; Immunoglobulins ; RNA Interference ; RNA, Messenger ; RNA, Small Interfering ; TYK2 Kinase ; Transfection

Objective:To explore the clinical characteristics and early diagnosis of cerebral infarction in critically ill children.Methods:The clinical data of the children who were diagnosed with cerebral infarction in PICU at Shengjing Hospital of China Medical University from January 2013 to September 2019 were analyzed retrospectively.Results:There were 27 children in this study, including 15 males and 12 females.The age of onset ranged from 1 month to 13 years old, with age of 3.0(0.7, 8.0) years old.Among 27 cases, there were ten cases of infection(37.0%), six cases of trauma(22.2%), three cases of congenital heart disease (11.1%), two cases of cerebrovascular disease (7.4%), one case of diabetic ketoacidosis(3.7%) and two cases of autoimmune factors(7.4%). The cause was unknown in three cases(11.1%). The main clinical manifestations were convulsion in 11 cases(40.7%), status convulsion in five cases(18.6%), limb dysfunction in four cases(14.8%), disturbance of consciousness in fvie cases(18.5%) and facial paralysis in four cases(14.8%). Among 27 cases, mechanical ventilation was performed in 12 cases (44.4%). The levels of D-dimer in all children were increased in varying degrees.After improvement and discharge, two children developed secondary epileptic sequelae.Head CT examination was performed in 20 cases, of which 11 cases showed definite cerebral infarction, eight cases of temporal lobe lesions and three cases of basal ganglia lesions.MRI+ MRA examination of the head in 27 cases showed that there were cerebral infarction and the infarct area large.Conclusion:The high incidence age of cerebral infarction in critically ill children is infantile.The most common diseases are infection, trauma, congenital heart disease, and the first clinical manifestations are convulsion and coma.Imaging examination showed that a high proportion of children had large area infarction, and the diagnostic effect of MRI is better than that of CT.D-dimer may be helpful for early clinical diagnosis.