Objective To evaluate the performance of the MAKER IS1200 automated chemiluminescence analyzer .Methods A series of experiments were designed to evaluate the precision ,reagent open bottle stability ,linearity range and reference interval of the MAKER IS1200 automated chemiluminescence analyzer in 8 items of hepatitis B 5 indexes ,AIDS ,hepatitis C and syphilis ac‐cording to the requirements of CLSI documents ,the detection results were performed the methodological comparison with those de‐tected by Abbott i2000 automated chemiluminescence analyzer .Results Except the total precision of HBEAG low value was slight‐ly higher than 15% of the judgment standard ,other indexes conformed to the requirements ;the open bottle stability of various items of reagent was better ;the quantitative item HBSAB had good linearity within the detection range (r2 >0 .95);in the verification of reference interval ,no outlier was found in each item ;in the methodological comparison ,the other items had good correlation with those detected by the ABBOTT i2000 except anti‐HCV .Conclusion The MAKER IS1200 automated chemiluminescence analyzer has good detection performance and meets the requirements of immunoassay detection in laboratory work .

BACKGROUND:There are some poisonous and hazard materials released from decoration materials and office furniture that will deteriorate the air of office if the ventilation of air-condition system is not very good. OBJECTIVE:To investigate the effects of newly decorated air-condition office to human health. DESIGN:A retrospective observational comparative study taking the office staff in newly decorated air-conditioned environment as the subjects and the office staff in natural ventilation environment with same decoration as the controls. SETTINGS:Occupational medicine department of a disease control and prevention center of a city. PARTICIPANTS:This study was conducted in the Department of Occupational Medicine of Dalian Centre for Disease Control and Prevention from April to May 2001.Inclusive criteria:office staff in a newly decorated and airconditioned telecommunication building of either sex;Exclusive criteria:people with acute or chronic disease history.Twenty-three office staff working in the airconditioned office was chosen as the observational group,the ratio of male to female was 12 to 11,aged from 24 to 51 with the mean age of 33.1 years old,average length of service of 14.2 years and average working length in air-conditioned office of 6.8 months.Other 23 office staff was randomly selected from natural ventilation office in the same building with the same decoration condition,the ratio of male to female was 10 to 13,aged from 22 to 58 with mean age of 36 years old, average length of service of 16.7 years and working length in this office for 8.2 months. INTERVENTIONS:To monitor the volatile organic compounds,hazard substance and microclimate in the decorated office with air-conditioner and conduct investigation to the health status of staff. Subjective symptoms between staff in different offices. RESULTS:The concentration of formaldehyde and ammonia exceeded the national hygienic standards in both air-conditioner and naturally ventilation offices,in which formaldehyde exceeded 6.1 and 2.3 times respectively while ammonia exceeded 8.5 and 2.3 times respectively.The illumination,assessment index of positive and negative ions and total bacteria in office with air-conditioner were lower than those of natural ventilation office (P< 0.01).The symptoms manifested in staff working in air-conditioned office such as dizziness,headache,chest pain,dry eye,decreased vision and menoxenia were more distinct than control group(P< 0.01).Dry mouth and hair losing were also more popular in air-conditioned group(P< 0.05). CONCLUSION:More attention should be paid to choosing environmental friendly building and decoration materials and office furniture when decorating.It is very important to make sure the ventilation of air-conditioner is normal and there is enough air change flow.

Objective To investigate the effects of apoptosis, Fas/FasL and Bax/Bcl-2 in rat liver allograft immune response. MethodsDifferent rat allograft models were established:(1)Acute liver allograft rejection group: PVG liver to Lewis rats; (2) Liver allograft tolerance group: PVG liver→DA rats; (3) F1(P/D)→D group: F1(PVG-DA) liver to DA rats; (4)HLTx(P/2→D)group: PVG/2 liver→DA rats. Liver samples were collected on days 1, 3, 5, 7 postoperatively (each n=3). Apoptosis was monitored by TUNEL(terminal deoxynucleotidyl transferase(TdT) mediated dUTP nick-end-labeling). The expressions of Fas/FasL and Bcl-2/Bax mRNA were examined by means of immunohistochemistry and in situ-hybridization respectively. Results Compared with liver allograft tolerance group, in acute liver allograft rejection group, the apoptosis cell numbers increased in hepatocytes, FasL expression was high in infiltrated cells in portal tract areas and low in hepatocytes, and the expression of Fas and Bax mRNA increased. The expression of molecules detected in F1(P/D)→D group was similar with those in acute liver allograft rejection. The expression of molecules detected in HLTx(P/2→D)group was similar with those in liver allograft tolerance group. On the fifth day postoperatively, the apoptosis index in portal tract areas had a positive correlation with the expression of FasL in hepatocyte and a negative correlation with the expression of Fas and Bax. Conclusions The high expression of FasL in infiltrated cells of portal tract areas, Fas and Bax in hepatocytes may be involved in rejection response. The high expression of apoptosis in infiltrated cells of portal areas, FasL in hepatocytes may relate with the establishment of tolerance.

Objective To investigate the effects of Yanshu injection for the chemotherapy, toxicity- reducing and immunization in mice implanted tumor. Methods Bulid up the mice models with S180 solid tumor transfered by ascites tumor, and observe the effect on tumor weight, blood WBC and bone marrow nucleated cells when combined use Yanshu injection and cyclophospamide (Cy). Determine OD value of plague forming cell by spectrophotometry, measure T-lymphocyte transformation by 3H-TDR incorporation, and survey natural killer cell (NKC) activity by colorimetric method, all these can help to study the role of Yanshu injection on the immune effect in the mice models. Results Yanshu injection can strengthen antitumor activity of Cy, antagonize the inhibition of Cy to WBC and bone marrow nucleated cell, improve OD value of plague forming cell, promote T-lymphocyte transformation, also can enhance NKC activity and killing rate. Conclusion Yanshu injection can increase the chemotherapeutic effects, reduce toxin, and enhance immune function of mice implanted tumor.

Objective To study the effects of apoptosis associated factors Fas/Fas, Bcl-2/Bax, adhesion molecules including ICAM-1, VCAM-1, PECAM-1, P-selectin, L-selectin and E-selectin in rat heart allograft immune response at different time points. Methods Rat acute cardiac allograft rejection model (PVG heart to DA rats) were established. Heart samples were collected on the day 1, 3, 5, 7 postoperatively ( n =3 in each group). Apoptosis was monitored by TUNEL. Fas, FasL, ICAM-1 and Selectin was examined by means of immunohistochemistry. The expression of Bcl/Bax mRNA, VCAM-1 mRNA, PECAM-1 mRNA was detected by means of situ-hybridization. Results In acute cardiac allograft rejection group, the expression of apoptosis, Fas/FasL, Bax, ICAM-1, VCAM-1 and PECAM-1 was increased. Apoptosis, Fas/FasL and Bax were mostly detected in cardiomycotes and ICAM-1, VCAM-1 and PECAM-1 were mostly detected in vascular endothelium and infiltrated cells. There was no expression of apoptosis associated factors and adhesion molecules in normal rat heart.Conclusion The high expression of apoptosis, Fas/FasL, Bax, ICAM-1,VCAM-1, PECAM-1 may be related with cardiac allograft rejection.

Objective To compare the efficacy and adverse reactions of TA regimen (docetaxel +epirubicin) and immunization therapy of TA regimen combined with dendritic cell (DC)-cytokine induced killer cell (CIK) in treating the patients with metastatic breast cancer (MBC).Methods Clinical data of 43patients with MBC received treatment in our hospital from January 2011 to January.2013 were retrospectively analyzed.Patients included were divided into two groups according to the treatment,simple treatment group (TA regimen,control group,22 cases) and combined treatment group (TA regimen + DC-CIK cell,observation group,21 cases).The curative effect,improvement situation of clinical symptoms,adverse reactions and changes of T cell immune phenotype in peripheral blood of patients before and after treatment in the two groups were compared.Results The total efficiency of treatment in observation group (85.7％) was higher than that in control group (63.6％),and the difference was statistical significant (x2 =4.949,P =0.026).Among the incidences of all kinds of adverse reaction appeared during the treatment in two groups,the white blood cells count decreased was the highest in observation group (76.2％) and in control group (59.1％),the difference had no statistical significance (x2 =0.858,P =0.36).After treatment,the rates of fatigue,loss of appetite,insomnia and night sweats of patients in observation group were lower than those in control group (23.8％ vs.54.5％,x2 =4.246,P=0.04;19.0％ vs.54.5％,x2 =5.795,P=0.02;19.0％ vs.50.0％,x2 =4.532,P=0.03).TheCD3+(60.4±12.3vs.47.4±12.8,t=3.393,P＜0.01),CD3+/CD4+(41.7±9.6 vs.28.1±10.5,t=5.442,P＜0.01),CD4+/CD8+ (1.5 ±0.2 vs.1.0±0.2,t =8.195,P＜0.01),NK cells(27.35.9vs.15.3±6.1,t=6.643,P＜0.01),NKTcells(14.7±l.4vs.6.0±1.2,t=11.020,P ＜ 0.0l) after treatment in observation group were obviously higher than those in control group.Conclusion Comparing with the pure TA scheme,the curative effect of immunization therapy of TA regimen combined with DC-CIK cell in treating the patients with MBC is better.It significantly improves the immune functions of patients with MBC,and does not increase adverse reaction.

Objective To investigate the efficacy of dexmedetomidine as an adjunct to combined intravenous-inhalational anesthesia for removal of foreign body in the trachea in children.Methods Sixty ASA Ⅰ or Ⅱ children,aged 1-4 yr,weighing 8-23 kg,were randomly divided into 2 groups(n =30 each):combined intravenous-inhalational anesthesia group(group Ⅰ)and dexmedetomidine as an adjunct to combined intravenous-inhalational anesthesia group(group Ⅱ).In group Ⅱ,8％ sevoflurane was inhaled by mask to induce sleep after entering the operating room,and the concentration was reduced to 4％ after the children sere asleep.Anesthesia was maintained with iv infusion of dexmedetomidine 0.5 μg/kg,and then iv injection of propofo1 2 mg/kg,followed by iv infusion of propofol at 6 rag· kg-1· h-1 and remifentanil at 0-15 μg· kg-1 ·min-t.In group Ⅰ,dexmedetomidine was not used and the other procedures were the same as those in group Ⅱ.Sevoflurane inhalation was stopped 2 min later and the rigid bronchoscope was inserted.HR and SpO2 were monitored and recorded before insertion and at 1 and 5 min after insertion.Complications such as respiratory depression,laryngeal edema,and bradycardia were reconded.The amount of propofol and remifentanil consumed,satisfactory level of bronchoscopy,and emergence time were recorded after operation.Results Compared with group Ⅰ,HR was significantly decreased at 1 and 5 min after insertion,SpO2 was significantly increased at 1 min after insertion,the amount of propofol and remifentanil consumed was significantly reduced,the operation time was significantly shortened,the emergence time was significanlly prolonged,the satisfactory level of bronchoscopy was significandy increased,and the incidence of respiratory depression and laryngeal edema was significantly decreased in grmp Ⅱ(P ＜ 0.05).Conclnslon The efficacy of dexmedetomidine as an adjunct to combined intravenous-inhalational anesthesia is better than that of combined intravenous-inhalational anesthesia for removal of foreign body in the trachea in children,with fewer complications.

Objective To investigate the effect of estrogen on regression of vascular calcification in rats induced by vitamin D3 plus nicotine.Methods Ninety-six female SD rats were divided randomly into control group (n=24) and calcification group (n=72).Vascular calcification of 72 rats was induced by vitamin D3 and nicotine (VDN).On the day 1,the VDN group rats were injected with vitamin D3(300 000 U/kg,i.m),and were intragastric administrated with nicotine (25 mg/kg),after 9 hours,another dosage of nicotine was given again.After 4 weeks,the VDN group rats were subdivided randomly into 4 groups:VDN group(n=16),Sham operation group (n=16),ovariotomy group (n=16),estrogen group(after ovariotomy,17β-estrogen was subcutaneously injected,50 μg· kg-1· d-1,n=16).Results After 4 weeks,the VDN group showed obvious vascular calcification,and calcium content of the vessel wall was significantly higher than that of control group (P＜0.01).Extensive calcification was witnessed on the aortic tunica media of the VDN group.After 12 and 8 weeks,the calcium content of the vessel wall in each subdivided groups was significantly lower than that at 4 weeks point(P＜0.01),and the lowest calcinm content was in estrogen group,meanwhile the reduction of previously accumulated arterial calcium precipitate in each group was different.Conclusions It is a reversible process that vascular calcification induced by vitamin D plus nicotine in rats.Estrogen can promote the regression of vascular calcification.

0.05);the total costs of L 1 group and L 2 group were424.76yuan and270.90yuan respectively,the cost of L 1 group was58%,higher than that of L 2 group.CONCLUSION:Considering from the perspective of pharmacoeconomics,L 2 group was a better one.

BACKGROUND: Apoptosis secondary to ischemia and hypoxia is the main cause of spinal cord dysfunction. Because of the decrease in atmospheric pressure, patients living on the Qinghai-Tibet Plateau are in a hypoxic environment, which is very unfavorable for the recovery of spinal cord injury. Hyperbaric oxygen therapy can improve the postoperative function of patients with incomplete spinal cord injury, and its effect is better on the plateau than at normal altitudes.OBJECTIVE: To observe the effect of hyperbaric oxygen therapy on traumatic spinal cord injury in patients living on the Qinghai–Tibet Plateau.METHODS: This prospective, open-label, randomized controlled clinical trial was performed at the Department of Spine Surgery, Affiliated Hospital of Qinghai University, China. In total, 164 patients with incomplete traumatic spinal cord injury were equally and randomly assigned to a control group and a hyperbaric oxygen therapy group. Patients in the control group were treated with pedicle screw fixation and decompressive laminectomy. In addition to the surgical treatment performed in the control group, patients in the hyperbaric oxygen group underwent hyperbaric oxygen therapy at 0.2 MPa once a day for four treatment courses. Ten treatment sessions constituted one course, and each course was separated by a 5- to 7-day rest interval. The primary outcome was the modified Barthel index to assess activities of daily living. The secondary outcomes were the American Spinal Injury Association (ASIA) impairment scale grade, sensory score, and motor score. The study protocol was approved by the Ethics Committee of the Affiliated Hospital of Qinghai University, China (Approval number: QHC011K). Written informed consent was provided by a relative or legal representative of each patient after they had indicated that they fully understood the treatment plan. RESULTS AND CONCLUSION: The partial results demonstrated that after four treatment courses (55-61 days), the modified Barthel index and ASIA tactile, pain, and motor scores were higher in the hyperbaric oxygen group than in the control group. The ASIA grades were significantly different between the hyperbaric oxygen group and control group. The proportion of patients with ASIA grades D and E was higher in the hyperbaric oxygen group than in the control group. In this trial, we aim to determine the efficacy of hyperbaric oxygen therapy on the treatment of incomplete traumatic spinal cord injury in patients living on the plateau and to provide clinical evidence for treating incomplete traumatic spinal cord injury in these patients.