Immune thrombocytopenic purpura is the common hemorrhagic disease in childhood.According to the international working group consensus,the article reviews its definition,classification criteria and the clinical features and diagnosis,in regarding the pathogenesis of immune function abnormalities,infection,platelets apoptosis and genetic background,espeially in children's treatment.

Sixty patients with dysphagia following the acute stroke were enrolled and divided into training group and control group with 30 cases in each group.Both groups received the routine medication,but patients in training group had additional swallowing rehabilitation training.After 4 weeks,the recovery rate of swallowing function in training group was higher than that in control group [ 93 ％ (28/30) vs.63 ％(19/30),P ＜ 0.05 ] ; the incidence rate of aspiration pneumonia in training group was lower than that in control group[ 3.3％ ( 1/30 ) vs.26.7％ ( 8/30 ),P ＜ 0.05 ].The results showed that swallowing training applied to acute stroke patients with dysphasia may promote the recovery of swallowing function,reduce the complications and improve their quality of life.

Objective To discuss the best way of gastric lavage in patients with drug intoxication.Methods Patients with drug intoxication(128 cases)were divided into the observation group(68 cases)and the control group(60 cases).The observation group adopted modified method,left lateral decubitus without pillow,plastic mouth-gap to assist intubation,the tube Was prolonged 10 to 15 cm after successful intubation,then repeated gastric lavage with little lavage liquid was carried out under low pressure.The control group was treated with routine method.One-time success rate of intubation,the incidence of adverse reaction and complications were observed and compared between the two groups.Results One-time success rate of intubation was higher,and the incidence of adverse reaction and complications were lower in the observation group compared with those of the control group.Conclusions The modified gastric lavage method,that is,left lateral decubitus without pillow,plastic mouth-gap to assist intubafion,the tube was,prolonged 10 to 15 cm after successful intubation,then repeated gastric lavage with little lavage liquid,is better than the routine method in the following aspects,higher one-time success rate of intubation,rapid gastric lavage,patency of drainage,little stimulation and injury of gastric mucosa,light adverse reaction and complication and better lavage effect.

Objective To observe the clinical curative effect of edaravone on treatment of acute lagre area cerebellar irdarction(ALACI). Methods 31 ALACI patients attacked within 72h were randomly assigned to therapygroup( n = 16) and control group( n = 15). Therapy group took the basic treatment as well as edaravone infused at a dose of 30mg,twice a day for 14 days. Control group took similar treatment to therapy group expect for edaravone.At 0th ,7th, 14th ,21th day after treatment, the C.SS and ability of daily living(ADL) were used to evaluate the recovery of neurological functions. Results Significant difference of CSS and ADL grading were detected between therapygoup and control group at 7th, 14th day( P < 0.05 ), with lower grading in therapy group ; there were significant differ-ence of CSS and ADL grading between therapy goup and control group at 21th day( P < 0.01 ), with lower grading in therapy group. No evident side effect was detected in edaravone therapy group. Conclusion Edaravone is a safe a-gent. It can effectively improve the neurological deficits and daily living ability of ALACI patients.

Objective To observe the efficacy and safety of BiPAP ventilator combined with nikethamide for the chronic obstructive pulmonary disease (COPD)complicated with type II respiratory failure.Methods 76 COPD patients complicated with type II respiratory failure were selected as the research subjects,they were randomly divided into two groups by single blind and randomized method,38 cases in each group.The control group was treated with nikethamide therapy,while the observation group used BiPAP combined with mechanical ventilation therapy.The clinical therapeutic effect and adverse reactions were analyzed and compared in the two groups.Results The total effective rate in the observation group was 92.11%,which was significantly higher than 68.42% in the control group (χ2 =10.54,P <0.05).After treatment,the HR and RR of the observation group were (94.28 ±7.62)/min, (13.27 ±4.41)/min,which were significantly decreased than before treatment (t =8.02,8.04,all P <0.05),and which were significantly lower than those in the control group (t =7.54,6.88,P <0.05).After treatment,the pH (7.39 ±0.06),PaO2 (96.31 ±5.44)mmHg,SaO2 (98.42 ±6.87)% in the observation group were significantly increased (t =7.56,7.76,7.45,all P <0.05),PaCO2 (57.36 ±4.98)mmHg was significantly decreased (t =7.54, P <0.05),which had significant differences with the control group (t =6.56,7.02,6.73,7.32,all P <0.05).There was no significant difference in the incidence of adverse reactions between the two groups (χ2 =2.14,P >0.05). Conclusion BiPAP ventilator combined with nikethamide meters has remarkable curative effect and high safety in treatment of COPD patients complicated with type II respiratory failure,which is worthy of clinical reference and popularization.

Objective To evaluate the efficacy and safety of calf pulmonary surfactant for injection on respiratory distress syndrome (RDS) in full term infant of cesarean section.Methods A total of 30 RDS in full term infant of cesarean section were divided into 2 groups according to family members intend.A group with 17 cases was given endotracheal drip into the calf pulmonary surfactant for injection and breath machine treatment according to the illness needs.B group with 13 cases was given the same treatment with A group except for calf pulmonary surfactant for injection.The changes of blood gas analysis before and after treatment,mechanical ventilation time,X-ray recovery time and hospital stays were compared between 2 groups.Results Arterial blood oxygen partial pressure after treatment in A,B group were (75.6±16.2),(54.8±12.4) mmHg (1 mmHg=0.133 kPa),and there was significant difference between 2 groups,t=3.84,P＜0.01.The incidence of cure,ventilation related lung injury and oxygen time needs,hospital stays were 16/17,1/17,(3.2±1.4) d,(7.2±1.8) d in A group,and 7/13,6/13,(5.6±2.1) d,(14.7±2.4) d in B group,and there were significant differences,P＜0.05 or ＜0.01.Conclusion Calf pulmonary surfactant for injection in treatment of RDS in full term infant of cesarean section,which is safe and effective and is worthy of promotion.

Objective To investigate the efficiency and safety of self-made Neiyixiao decoction combined with leuprorelin in patients with endometriosis (EMs). Methods A total of 82 patients with EMs were randomly recruited into a treatment group and a control group, with 41 patients in each group. The treatment group was treated with self-made Neiyixiao decoction combined with leuprorelin, and the control group was treated with leuprorelin only. The serum levels of CA125 and RANTES were detected with enzyme-linked immunosorbent assay. The symptoms and signs scores for pelvic endometriosis were evaluated before and after the treatment. Results The symptoms scores (0.4 ± 0.2 vs. 0.6 ± 0.3;t=3.552, P=0.001) and the signs scores (0.8 ± 0.4 vs. 1.1 ± 0.6;t=2.664, P=0.009) for pelvic endometriosis after the treatment in the treatment group were significantly lower than those in the control group. The serum levels of CA125 (26.4 ± 11.9 U/ml vs. 33.9 ± 12.8 U/ml; t=2.012, P=0.048) and RANTES (34.3 ± 14.1 ng/L vs. 43.7 ± 16.8 ng/L;t=2.744, P=0.008) after the treatment in the treatment group were significantly lower than those in the control group. The total effective rate in the treatment group was significantly higher than that in the control group (90.2%vs. 73.2%;χ2=3.998, P=0.046), and the rate of adverse reaction significantly lower (7.3%vs. 24.4%;χ2=4.479, P=0.034). The recurrence rate in the treatment group was significantly lower than that in the control group (16.2%vs. 46.7%;χ2=5.954, P=0.015) during 12 months of follow-up. Conclusion Self-made Neiyixiao decoction combined with leuprorelin can decrease serum levels of CA125 and RANTES, alleviate symptoms and signs, and its efficiency and safety are superior to leuprorelin alone in patients with endometriosis.

Neonatal candida colonization is the most important risk factor of neonatal invasive candidiasis.Knowing the characteristics is conducive to recognize the candida colonization early and evaluate the risks of IC.The article reviews the incidence,time,place,trains,transmission,risks of neonatal candida colonization and the relation between candida colonization and IC in neonatal wards,providing the evidence for neonatal IC prophylaxis.

Objective:To develop a real time fluorescent quantitative reverse transcriptase polymerase chain reaction(RT-PCR) system for determining the expression of Dicer mRNA in human hepatoma cell lines and 20 samples of primary hepatocellular carcinoma(PHC)tissues.Methods: The specific primers,designed according to the complete sequence of Dicer mRNA,and the fluorescence dye SYBR Green Ⅰwere used for RT-PCR amplification.The fluorescence was monitored in a real time manner.The expression levels of Dicer mRNA in samples were calculated according to the standard curve and the nonspecific amplifications were excluded by melting curve analysis.The mRNA levels of Dicer were presented as the ratios of Dicer mRNA to 18S rRNA.Results: The linear detection range of this system was from 5?10~(2)to 5?10~(9)copies/?l and the coefficient of variation values for intra-experimental and inter-experimental reproducibility ranged from 4.13% to 19.72% and from 6.25% to(18.76%,) respectively.The expression levels of Dicer mRNA in HBV positive hepatoma cell line HepG2.2.15 or in HBV negative hepatoma cell line HepG2 were significantly lower(P

Objective To observe the preventive effect of early rehabilitation intervention and intermittent pneumatic compression therapy device (IPC) combined with low molecular weight heparin for cerebral hemorrhage deep vein thrombosis (DVT) formation,thus to provide basis for clinical intervention.Methods 124 patients with cerebral hemorrhage were selected,and they were randomly divided into the observation group and control group,62 cases in each group.The control group received low molecular weight heparin and routine intervention,the observation group received early rehabilitation intervention and IPC treatment on the basis of control group.The venous blood hemorheological indicators were detected,and the incidence of DVT was recorded.Results The whole blood viscosity and D-dimer of the observation group after intervention were (4.17 ± 1.12)mPa/s and (214.84 ± 31.40)ng/mL,which were lower than (5.21 ± 1.08) mPa/s and (331.90 ± 35.38) ng/mL of the control group,the differences were statistically significant(t =8.52,8.90,all P ＜ 0.05).The incidence rate of DVT of the observation group was 1.64％ (1/62),which was lower than 17.74％ (11/62) of the control group,the difference was statistically significant(x2 =9.17,P ＜ 0.05).Conclusion Early rehabilitation intervention and IPC combined with low molecular weight heparin for preventing cerebral hemorrhage DVT formation can significantly increase the venous blood flow velocity,reduce blood viscosity,decrease the incidence of DVT.It has better clinical value for the prevention of DVT formation of cerebral hemorrhage.