To investigate the outcome of renal transplantation in the elderly patients,the sur-vival rate,postoperative complications,rejection incidences and causes of death after renal trans-plantation in the elderly patients were compared to those of the contemporary younger patientsduring a follow-up of 5 years.No significant difference was found in the patients/graft survivaljection incidence was only 16%6 months after renal transplantation.Infection was the most com-mon complication and the main cause of death.The findings suggest that the elderly patients aresuitable for the renal transplantation as younger ones.

Objective To study the mechanism of the Compound Qinbai Granule (Granules composed of Scutellaria root and Phellodendron bark) in the treatment of radioactive dermatitis in rats.Methods The rat models of radioactive dermatitis were established and randomized into three groups:Compound Qinbai Granule group (experimental group),dexamethasone ointment group (traditional group),and normal saline group (control group).The wound was treated with Compound Qinbai Granule spray,dexamethasone ointment external application,and normal saline spray respectively.By immunohistochemical staining and image analysis,the growth of granulation tissue in the wound area wound healing rate,and the expression of basic fibroblast growth factor (bFGF) in the wound tissues were determined on the second,sixth,tenth,eighteenth,and twenty-fourth day after injury.Results The average number of granulation tissue and wound healing rate in the experimental group on the sixth,tenth,eighteenth,and twenty-fourth day were all higher than those in the traditional group and control group with a significant difference (P

Objective To seek an approach to differentiate between tumors from parotid space and from parapharyngeal space on axial CT or MR imaging. Methods Forty-five patients with tumors originated at the parotid or parapharyngeal space were reviewed retrospectively. The diagnosis was confirmed pathologically in all of the cases. The correlation among neoplasms and the posterior belly of digastric muscle and its shifting situation were evaluated on axial CT or MRI scans by two senior radiologists. All of the estimations were compared with the results of operation. Results Twenty-seven tumors from parotid space were located in the superficial side to the posterior belly of digastric muscle and pushed it medially. On the contrary, 18 neoplasms from parapharyngeal space were situated at the profound side to the posterior belly and shifted it laterally. Conclusion Tumors superficial to the posterior belly of digastric muscle and pushing it medially indicate that they come from parotid space. While neoplasms profound to the posterior belly of digastric muscle and shifting it laterally mean that they come from parapharyngeal space.

Objective To probe into the role of apoptotic cells in the disease development of systemic lupus erythematosus.Methods Apoptotic thymocytes injected intraperitoneally into syngeneic normal C57 mice and lupus prone BXSB mice,and then the level of anti dsDNA and anti ssDNA antibodies of IgM and IgG classes,and the concentration of urine protein and immune complex of IgG class deposited in kidney were detected.Results The apoptotic thymocytes induced the production of anti dsDNA and anti ssDNA antibody of IgG class in C57 mice,and their levels were higher in female BXSB mice than in male ones.The concentration of urine protein of male BXSB mice injected with apoptotic thymocytes was noticeably higher than that of the control male BXSB mice.Conclusion Apoptotic cells have immunogenicity,and may be the major source of autoantigens in lupus BXSB mice.Lupus genetic factors and Yaa gene can potentiate the immunogenicity of apoptotic cells or accelerate their inducing the lupus nephritis.

Objective To diagnose Chinese autosomal dominant polycystic kidney disease (ADPKD) rapidly by detecting microsatellite SM7. Methods Microsatellite SM7 was amplified by modified PCR in two temperatures. Gene linkage analysis was performed. Rusults DNA analysis predicted 9 asymptomatic members as PKD1 gene carriers. Conclusion The presymptomatic gene diagnosis could be done rapidly by detecting microsatellite SM7 with modified PCR amplification in two temperatures.

Objective To select a safe and feasible way by comparing the two main styles of splitting liver transplantation classical splitting and modified splitting.Methods In order to compare the distribution of artery, vein and bile duct of all segments of liver, 58 formaldehyde fixed livers and 8 fresh livers were divided into two groups. One group was subjected to classical splitting and the other group to modified splitting. Results Among 66 livers, 66.7?% of the segment VIII and 100?% of the segment V (all or some) drained to middle hepatic vein. In classical splitting group, the vein drainage of 5th and 8th segments of right graft was destroyed.Conclusion Modified splitting is safer and more reliable than classical splitting, so the former is the first choice when the size of donor liver is suitable.

Objective To study the changes of system immune and intestinal immune in the progression of non-alcoholic fatty liver disease due to obesity. Methods Ninty male SD rats were divided into control, high-sucrose and high-fat diet groups. Non-alcoholic fatty liver disease models were established by feeding with high-sucrose diet or high-fat diet and were killed at the 4th,8th and 12th weeks with 10 each for each group. The extent of liver steatosis was observed with HE staining.Portal blood endotoxin level was assessed by limulus test. The percentage of CD4+ and CD8+ cells in peripheral blood mononuclear cells (PBMC) and lymphocytes in Peyer's patches (PP) were calculated by flowcytometry. Results In comparison with control group, the endotoxin level was not elevated from the 4th week to 12th week in high-sucrose diet group, (all P values＞0.05), but was increased in high-fat diet group at the 8th week (P＜0.05). CD4/CD8 ratio in PBMC was higher in high-sucrose and higt-fat diet groups than that in control group at the 4th week (P＜0. 05) ,but was lower than that in control group at the 8th and 12th weeks (P＜0. 05). Whereas the variation of CD4/CD8 ratio in PP was consisted with that in PBMC between the high-sucrose and high-fat diet groups at the 4 th and 8 th weeks, but there was no difference when compared with control group at the 12 th week (P＞0.05).Conclusion Obesity can inhibit systematic immune and intestinal immune. The intestinal immune may be regulated by the liver.

Objective: To observe the preventive effect of taurine on alcoholic liver damage in rat.Methods: The model was induced by filling stomach with alcohol, and the treatment groups were given two different concentrations of taurine, and the control group was given 0.9%NS, then we measured the blood triglycerides (TG), and the pathological changes of liver quantitatively.Results: Two different le-vels of taurine can significantly decrease the level of TG and ameliorate the liver damage of rats (P0.05), and the 5% taurine group can also decrease the mean arterial pressure (P

ObjectiveTo investigate the effects and mechanisms of IL-32γ-shRNA-mediated gene silencing on the proliferation and apoptosis of fibroblast-like synoviocytes in patients with rheumatoid arthritis (RA).MethodsA eukaryotic expression plasmid of shRNA targeting IL-32γ was transfected into fibroblastlike synoviocytes by liposome in patients with rheumatoid arthritis.RT-PCR was used to determine the expression level of IL-32γ.Western blotting was used to detect the levels of cyclin D1 and p-Akt.The proliferation of RA-FLS was examined by MTT.Cell cycles were analyzed by flow-cytometry.The apoptosis of cells were measured by TUNNEL.Comparisons between groups were tested by t test.Results ① The expression of IL-32γwas significantly inhibited by shRNA-IL-32γ-expressing plamid PGCsi 3.0 targeting sequence 1,2 and 3,and the inhibition rate had reached 75.6％,66.2％ and 64.1％,respectively.② The absorbance value of proliferation of RA-FLS in EASY-shRNA-IL-32γ group was significantly lower than that in the shRNA-control group and normal group at day 3 [(0.23±0.03) vs (0.35±0.03) and (0.36±0.04),P＜0.05] and 5 [(0.27±0.03) vs (0.52±0.05) and (0.53±0.04),P＜0.01 ] after transfection.③ The rate of RA-FLS at phase G1 in the EASY-shRNA-IL-32γ group was significantly higher than that in the shRNA-control group and normal group respectively [(88±6)％ vs (69±5)％ and (68±4)％,P＜0.05],while those at phase S+G2 in the EASY-shRNA-IL-32γgroup was significantly lower than that in the shRNA-control group and normal group [ ( 13.6±3.0)％ vs (30.2±4.1)％ and(32.1±4.3)％,P＜0.01].④The rate of RA-FLS apoptosis in the EASY-shRNA-IL-32γ group was significantly higher than that in the shRNA-control group and normal group[(20.50±3.21 )％ vs (9.20±0.32)％ and (8.60±0.22)％,P＜0.01].⑤ The expression of cyclin D1(0.36±0.04) and p-Akt(0.31±0.03) in the EASY-shRNA-IL-32γ group was significantly lower than that in the shRNA-control group [ (0.59±0.08) and (0.53±0.06)] and normal group [(0.61±0.07) and (0.52±0.06),P＜0.01].ConclusionEASYshRNA-IL-32γ can inhibit RA-FLS proliferation by down-regulating the expression of cyclin D1 and induce RA-FLS apoptosis by down-regulating the expression of p-Akt.

159 kidneys from infant and neonatal cadavers were dissected under stereoscope.The study showed that there were a lot of lymphatics(11.11?2.48) originating fromthe lymphatic network of renal sinus.The lymphatics of renal pedicle variedslightly in diameter (0.25-0.40 mm).The mean value of the diameter oflymphatics in the renal pedicle was 4mm.The mean value of the ratio ofdiameter of lymphatics to that of renal vein on the same side is 90.47%.Therewere positive correlation between them.The renal lymph nodes were scattered singlyin number in each kidney.