Objective To develop a portable analyzer which can rapidly detect antigen of type B hepatitis,antibody of HIV and H5N1 in blood samples.Methods The micro-processor of STC89C52 was adopted as the controlling core in the system.Based on an embedded technology,the analyzer can rapidly detect the antigen of type B hepatitis,antibody of HIV and H5N1 by processing the synchronic frequency of quartz crystal micro-array and by sensitivity of quality on the surface of quartz crystal with special identification between antibody and antigen.Results The system has the advantages of high sensitivity,better sample-specific characteristics,convenient portability and multi-parameter simultaneous detection.Conclusion The piezoelectric analyzer exceeds the traditional reagent method and serves for institution of scientific research and hospital widely.

Objective To evaluate the imaging characteristics of the bone infarct . Methods 13 cases of the bone infarct proved by clinical manifestation and surgical pathology were collected,there were 5 males and 8 females. All patients had X-ray films, 4 were imagedwith CT scanning , 7 were imaged with MRI , and 2 underwent all three techniques . The bone infarct were classified into the early,themiddle and later stages.X-ray , CT and MRI findings of bone infarct ,were analysed characteristics . Results Partial osteoporosin and punctate calcifications could be seen on X-ray and CT , in the early and middle stages , which presented as iso-/low-signal on T_1WIand iso-/high-signal on T_2WI in the center of the bone infarct with a tortuous low-signal on T_1WI and high-signal on T_2WI zonearound.In the later stages,the bone infarct was characterize by irregular sclerosis and tortuous calcification on X-ray and CT,and by low-signal on both T_1WI and T_2WI . Conclusion MRI is the most effective way to find the bone infarct in the early stages , X-ray is helpful in diagnosing the bone infarct in the middle and later stages , CT is more sensitive than X-ray . X-ray ,CT and MRI should be combined in diagnosing the bone infarct .

Objective To explore the imaging and pathological characteristics of the telangiectatic osteosarcoma.Methods The clinical,imaging and pathological data of telangiectatic osteosareoma in 12 cases were analysed.Results The tumors often located at the diaphysialepiphysial of the os longum and most of them were occured at the lower limb,increasing of the alkaline phosphatase(AKP) was rare.X-ray and CT character istics were osteoclastic and expensive bony destruction;there were multiple small cystic formation and fluid-fluid level in the destructive region and soft masess;the cortex of the bone became thinner with multiple sieve type destruction;the periosteal reaction and radial bone spicule were often seen.Pathological characteristics were of typical tumor cell of the osteosarcoma,mutliple sinus of the blood and "color bland shape" component.Conclusion The telangiectatic osteosarcoma has certain specific clinical,imaging and histologyical characteristics.

Morphine has been reported to suppress human immune response. We aimed to observe the effects of morphine, fentanyl and tramadol on NF-kappa B and IL-2 from both laboratory and clinical perspective. Jurkat cells were incubated with ten times clinically relevant concentrations of morphine, fentanyl and tramadol before being stimulated with PMA. NF-kappa B binding activity and IL-2 levels were measured. In the clinical study, 150 consenting patients were randomized into 3 groups according to the analgesics used in them, namely, group morphine (M), group fentanyl (F) and group tramadol (T). IL-2 was measured preoperatively and 1, 3 and 24 h after operation. Consequently, NF-kappa B activation was suppressed by morphine and fentanyl but not by tramadol. IL-2 was significantly decreased by morphine and fentanyl but not by tramadol in vitro. In the PCA patients, IL-2 was decreased in group M and increased in group F postoperatively. Whereas in group T, IL-2 was unchanged 1 h after operation but was significantly elevated 3 and 24 h after operation. Our results showed that the inhibition of morphine on IL-2 was most probably related to its suppression on NF-kappa B. Fentanyl had different effects on human immune response in vitro and in vivo. Tramadol may have immune enhancing effect.

Objective To evaluate which b value is the best to distinguish the glioma recurrence and radiation-induced brain injury using DWI when b value were at 1 000 s/mm2、3 000 s/mm2 and 5 000 s/mm2 respectively.Methods Retrospective analysis the DWI of 21 patients who suffered from glioma recurrence and radiation-induced brain injury obtained on a 3T MRI scanner.Results (1)All recurrent glioma (100%)showed hyper-intense signal,while most radiation-induced brain injury patients (80%)showed hypo-in-tense signal when b=5 000 s/mm2 .The sensitivity and specificity were high (100% and 80% respectively)when hyper-intense sig-nal was taken as a diagnostic point of glioma recurrence and radiation-induced brain injury.(2)The receiver operating characteristic (ROC)curve analysis suggested that the minimum ADC resulted in a highest sensitivity to differentiate glioma recurrence from radi-ation-induced brain injury when b=5 000 s/mm.Conclusion High b-value is more accurate to reflect cell density,and the minimum ADC is better to differentiate the glioma recurrence and radiation-induced brain injury when b=5 000 s/mm2 on DWI.

Objective To study the imaging characteristics of localized myositis ossificans for improving its diagnosis and differen-tial diagnosis ability.Methods The Clinical and radiographic data of 45 cases with localized myositis ossificans proved by pathology or clinical follow up were analyzed retrospectively,summarize its imaging features.Results Of the 45 cases,1 7 cases occurred in the elbow joint,1 5 in the hip joint,13 in the other parts of the body.X-ray showed lining or lamellar high-density ossification in soft tis-sues in 34 cases,of those 12 cases with “shell”ossification.Compared with the X-ray,CT showed more clear ossification.MRI showed the soft tissue mass with peripheral edema in 18 cases;3 cases obvious ossification,no edema around.SPECT/CT showed abnormal imaging agent concentration in soft tissue within 12 cases,of those 5 cases concentration range greater than the range of ossification,4 cases concentration range less than the range of ossification.Conclusion Localized myositis ossificans have certain im-aging characteristics.Integrated application of a variety of imaging combined with the clinical can fully display the evolution of the disease,and improve its diagnosis rate.

Objective To study the imaging characteristics of osteosarcoma.Methods 35 patients with osteosarcoma proved pathologically were examined by radiography,of them 25 cases were examined with CT scan or MRI,and 8 cases undergone contrast-enhanced MRI.The imaging features of osteosarcoma were analysed comparatively with that of pathology.Results The lesions localized at distal part of femur in 17 cases and proximal part of tibia in 8 cases,that were most location of this tumor.The imaging characteristics of osteosarcoma included bone destruction,neoplastic bone,periosteal reaction and soft-tissue mass,in some cases,epiphyseal plate and epiphysis were involved,even the joint face and the opposite osseous of joint(skip lesions).On enhanced MR scanning,the margin of tumor was enhanced in early and the center of tumor was delayed enhancement.CT could showed "sieve sign"and small neoplastic bone.Conclusion Osteosarcoma has certain imaging characteristics;X-ray examination is the first choice in diagnosing osteosarcoma,CT and MR could provide much more comprehensive imaging information for clinic.

Objective To analyze X-ray ,CT and MRI findings of Charcot’s joint.Methods 24 cases of Charcot’s joint were collected,there were 11 males and 13 females.24 cases were examined with radiography,9 with CT ,5 with MRI,and 2 were examined with all three techniques.X-ray,CT and MRI imaging findings of Charcot’s joint were analysed.Results There were two types of Charcot’s joint,hypertrophic(12 cases) and atrophic(12 cases).X-ray and CT findings of hypertrophic type were hyperostotic osteosclerosis with osteophytes,periarticular soft tissue swelling and ossification.While the atrophic type appeared as extensive bone resorption and disappearance,periarticular debris and soft tissue swelling,articular dislocation.These imaging findings of Charcot’s joint also could be showed by MRI.The joint capsule thickened periarticular soft tissue markedly enhanced after Gd-DTPA was administrated with definite medial margin.Conclusion Charcot’s joint is of typical imaging appearances.

Objective To explore the diagnostic value of SPECT/CT on fracture healing.Methods A total of 28 patients (13 males,15 females; average age 45.3 years) with fracture were enrolled.All underwent metal fixation.SPECT/CT scans were performed at least 6 months after the metal fixation.X-ray examination before SPECT/CT showed equivocal fracture healing.The fracture delayed union or nonunion were judged by degree of uptake as well as uptake pattern around fracture site.Final diagnosis was confirmed by surgery or follow-up X-ray film.Results Among 28 patients,15 showed delayed union (confirmed by follow-up),and 13 showed nonunion (confirmed by surgery).There were 13 screws in 6 patients showed abnormal uptake,suggesting screw loose (confirmed by surgery).Conclusions SPECT/CT imaging could be of clinical significance in assessing fracture healing.

Objective To investigate the change in 5-hydroxytryptomine (5-HT) content in spinal dorsal horn in a rat model of tibial bone cancer pain (BCP). Methods Sixty female SD rats weighing 160-180 g were randomly divided into 3 groups ( n = 20 each): control group (group C), sham operation group (group S) and BCP group. BCP was induced by intra-tibial inoculation of 10 μl Walker 256 breast cancer cell suspension in group BCP, while group S received intra-tibial inoculation of 10 μl D-hank solution. Paw withdrawal threshold to mechanical stimulation with yon Frey filaments (MWT) was measured 1 d before (baseline) and at 3, 5, 7, 9, 11,14, 16, 18 and 21 d after breast cancer cell inoculation. At 1 d before and 7, 14 and 21 d after breast cancer cell inoculation, four animals in each group were sacrificed after measurement of MWT. Their lumber segments of the spinal cord were removed for assay of 5-HT content in spinal dorsal horn using HPLC with fluorescence detector.HE staining was used to detect the damage to the tibia. Correlation between the 5-HT content and MWT was analyzed. Results MWT was significantly decreased after breast cancer cell inoculation in group BCP ( P ＜ 0.05).Microscopic examination showed serious bone destruction of tibia at the injection site in group BCP, while no bone destruction was found in groups C and S. 5-HT content in spinal dorsal horn was significantly higher in group BCP than in groups C and S (P ＜ 0.05). There was strong negative linear correlation between 5-HT content in spinal dorsal horn and MWT ( r = - 0.973, P ＜ 0.05 ). Conclusion The 5- HT content in spinal dorsal horn is significantly increased in rats with tibial BCP and is involved in the development of BCP.