ve To assess the protective effect of fentanyl on primary cultured myocardial cells against injury induced by anoxia-reoxygenation. Methods Ventricular myocardial cells enzymatically isolated and cultured in 1640 culture medium for 6 days were randomly divided into 5 groups: group A received no anoxia served as control; group B received 2 hours anoxia followed by half an hour reoxygenation; and group C, D and E received 10ng ? ml-1, 30 ng ? ml-1 and 50 ng? ml-1 fentanyl respectively before anoxia-reoxygenation. The cell viability, myocardial intracellular content of malondialdehyde (MDA) and the activities of lactic dehydrogenase (LDH) and creatine kinase(CK) were measured at the end of the experiment. Results Anoxia-reoxygenation caused dramatic decrease in cell viability, and increases in myocardial intracellular MDA content and the LDH and CK activities as compared with those in control group. Fentanyl 30 ng?ml-1 and 50 ng?ml-1 significantly attenuated the increases in LDH, CK activities and MDA content, and decrease in cell viability caused by anoxia-reoxygenation. Fentanyl 10ng?ml-1 did not produce any significant changes. Conclusions Fentanyl can produce protective effects on primary cultured cardiomyocytes against anoxia-reoxygenation injury.

Objective To develop a portable analyzer which can rapidly detect antigen of type B hepatitis,antibody of HIV and H5N1 in blood samples.Methods The micro-processor of STC89C52 was adopted as the controlling core in the system.Based on an embedded technology,the analyzer can rapidly detect the antigen of type B hepatitis,antibody of HIV and H5N1 by processing the synchronic frequency of quartz crystal micro-array and by sensitivity of quality on the surface of quartz crystal with special identification between antibody and antigen.Results The system has the advantages of high sensitivity,better sample-specific characteristics,convenient portability and multi-parameter simultaneous detection.Conclusion The piezoelectric analyzer exceeds the traditional reagent method and serves for institution of scientific research and hospital widely.

Objective:To enhance hospital equipment information management standards for the purpose of measurement instruments.Methods:Using solutions based on Web technology, database technology, database through the establishment of measurement devices, implement life cycle management method.Results: To solve the many hospital only attach importance to the management of medical equipment, ignore the metering device management, lead to measuring instruments record disorder, query the inconvenience problem.Conclusion: Medical device measurement equipment management system improves the ability of the hospital information management, strengthens the efficiency of communication and exchanges of various departments and produces good economic and social benefits.

Objective To determine the mimic epitope of N-methyl-D-aspartate receptor 2B subunit (NR2B). Methods The monoclonal antibody interacting with NR2B was used as target protein to screen the binding peptide from a 12-mer Ph. D random peptide library. After three rounds of affinity screening, twelve specific clones were selected randomly and identified by sandwich ELISA. The peptide sequences were analyzed by DNA sequencing. The clones containing a common sequence were named positive clone. The competitive inhibition of the native antigen bound to monoclonal antibody against the NR2B by the positive clone was assayed by cell ELISA. Results After 3 rounds of screening the phages specifically bound with mAb NR2B were selected and amplified. Nine of the 12 clones displayed a common aminoacid sequence: SHPPVMPWPTST. The inhibitory assay showed that the mimic epitope peptides displayed on the phage surface could effectively inhibit the combination of the monoclonal antibody with native antigen. The inhibitory rate of mimic epitope was (45?3)% .Conclusion The mimic epitope of NR2B was screened successfully from the 12-mer Ph D random peptide library. This peptide mimics an epitope of the native NR2B.

35%.The patients were premedicated with intramuscular phenobarbital 0.1g and atropine 0.5 mg. In group B and C 8-12 ml?kg-1 of blood was withdrawn from cubital vein in 10-15 min and equal volume of 6% hydroxyethyl was infused at the same speed simultaneously before anesthesia. In group C the blood shed during operation was collected and anticoagulated for reinfusion. Anesthesia was induced with fentanyl 4-6?g?kg-1 , propofol 2 mg?kg-1 and pipecuronium 0.1 mg?kg-1.The patients were mechanically ventilated after tracheal intubation and PETCO2 was maintained at 25-35 mm Hg. Anesthesia was maintained with 1%-3% isoflurane and intermittent iv boluses of fentanyl and pipecuronium. Lactated Ringer's solution was infused at 10-12 ml?kg-1.h-1 during operation in all patients. ECG, NIBP, SpO2, PETCO2 and CVP were monitored throughout anesthesia. Plasma protein, Hct and Hb were measured before and after ANH and after reinfusion of the shed blood. Neuromuscular function was assessed using a train-of-four stimulation (TOF). Onset time, peak effect time (when T1 decreased to 0), duration of action (from the end of pipecuronium injection to recovery of T1 to 25% of the control) of intubation dose and maintenance dose and recovery index (recovery of T1 from 25 % - 75%) were recorded.Results Demographic data including sex, age, body weight and height were not significantly different among the three groups. The vital signs were stable in the three groups. Plasma total protein and albumin concentrations, Hb and Hct decreased significantly after ANH in group B and C and were significantly lower than those in group A. After autologous transfusion there was no significant difference in plasma protein concentration and Hb between group A and B. Hb and Hct were significantly higher in group C than those in group A and B (P

Objective: To perform target-controlled infusion (TCI) in total intravenous anesthesia (TIVA) of propofol and fentanyl. Method: Using effect compartment modeling, computer controlled infusion(the computer software was developed by Coezee and Pina) was performed during induction and maintenance of anesthesia in two groups of adult patients. The target predicted concentration of theoretical effect-site compartment for propofol was 4?g/ml and for fentanyl was 2?g/ml. The plasma concentration (Cm) of propofol was determined by fluorospectrophotometry and Cm of fentanyl was measured with radioimmunoassay. Result: The mean Cm from 0 to 120 min showed that excessive dose of propofol was administered, MDAPE=25%,however the mean Cm of fentanyl was lower than the target level obviously,MDAPE=35.5% in first group. After an imitative calculation,another pharmacokinetic (PK) parameter sets of propofol and fentanyl were selected in the second group,MDAPE=15.5% for propofol and MDAPE=37. 75% for fentanyl. Conclusion: The concentrations of propofol and fentanyl in the effect site compartment can be achieved rapidly by using the effect compartment control algorithm. The PK parameter,described by different authors influences the accuracy of TCI administration.

This paper introduces such information of a LCD graphic display system based on SED1335 as its hardware link with the singlechip computer Atmega128 and the design of system display software. With a simple, compact and stable circuit, this system can be applied to portable medical signal monitoring and controlling equipments.

Objective To analyze X-ray ,CT and MRI findings of Charcot’s joint.Methods 24 cases of Charcot’s joint were collected,there were 11 males and 13 females.24 cases were examined with radiography,9 with CT ,5 with MRI,and 2 were examined with all three techniques.X-ray,CT and MRI imaging findings of Charcot’s joint were analysed.Results There were two types of Charcot’s joint,hypertrophic(12 cases) and atrophic(12 cases).X-ray and CT findings of hypertrophic type were hyperostotic osteosclerosis with osteophytes,periarticular soft tissue swelling and ossification.While the atrophic type appeared as extensive bone resorption and disappearance,periarticular debris and soft tissue swelling,articular dislocation.These imaging findings of Charcot’s joint also could be showed by MRI.The joint capsule thickened periarticular soft tissue markedly enhanced after Gd-DTPA was administrated with definite medial margin.Conclusion Charcot’s joint is of typical imaging appearances.

Objective To investigate the risk factors for postoperative nausea and vomiting (PONV) in spinal anesthesia patients.Methods A total of 841 patients received spinal anesthesia were visited after operation.Data were analyzed using univariate analysis and multivariate Logistic regression to identify risk factors related to PONV.Results PONV occurred in 94 patients (11.2％,94/841).Univariate analysis showed that PONV was unrelated with gender,age,ASA classification,anesthesia mode (P ＞ 0.05),related with operation department (P =0.026),body mass index (P =0.020),education level (P =0.000),history of previous surgery anesthesia (P =0.005),history of PONV (P =0.000),history of kinesia (P =0.002),smoke (P =0.019),intraoperative using of tramadol (P =0.018).Multivariate analysis showed that operation department (OR =4.039,95％ CI 1.331-12.259,P=0.048),education level (OR =3.504,95％ CI 1486-8.260,P=0.015),history of PONV (OR =5.113,95％ CI 1.790-14.606,P =0.002),intraoperative using of tramadol (OR =5.316,95％ CI 1.091-25.908,P =0.039) were identified as independent risk factors for PONV.Conclusions The independent factors associated with PONV following spinal anesthesia include operation department,education level,history of PONV,intraoperative using of tramadol.Identifying patients who are at high risk for PONV will enable the formation of more timely management project.

ObjectiveTo summarize and analyse the perioperative management especially theanesthesia of 20 kidney transplant recipients in non-transplant surgery. MethodThe anesthesia management of 20 kidney transplant recipients in non-transplant surgery was analyzed retrospectively. ResultsIn 20 cases, 1 case (5％) was performed under local anesthesia,4 cases (20％) were performed under intravertebral anesthesia and 15 cases (75％) were performed under general anesthesia. The operation time was 30-260 min, all cases were managed successfully. ConclusionIt is still a clinical challenge to deal with the surgical patients after kidney transplantation, and it needs fully understanding of the pathophysiological status of the patient and closely collaboration of transplant physicians, anesthesiologists and the surgeons.