Objective Research of Enterococcus faecalis and Enterococcus faecium to our hospital in patients with severeurinary tract infection status and to compare the resistance ,to provide a basis for clinical rational use of antimicrobial agents .Methods From 2011 January -2014 December in our hospital with severe urinary tract infections in patients with isolated 172 strains of En‐terococcus were analyzed ,with the French bioMerieux ATB microorganism identification instrument identification of Enterococcus , using the disc diffusion method (K‐B) was used in drug sensitivity test ,by using WHONET 5 .6 software test data analysis and processing .Results A total of 172 strains of Enterococcus ,the separation of Enterococcus faecalis and Enterococcus faecium rates were 68 .0% (117 strains) and 32 .0% (55 strains);drug sensitive test showed that two kinds of Enterococcus to Linezolid、vanco‐mycin、Ti Koa Laing、tigecycline are more sensitive (resistance rate < 5% ) ,in addition to these 4 drugs ,for the other 12 kinds of antimicrobial resistance has significant difference (P< 0 .05);there were 5 strains of vancomycin resistant Enterococcus ,the 3 strains of Enterococcus faecalis ,2 strains of Enterococcus faecium ,Nailinai temozolomide of 3 strains of Enterococcus faecalis ,En‐terococcus faecium strain .Conclusion Multi drug resistant Enterococcus isolated from our hospital in patients with severe in urine samples of serious ,there are two kinds of resistance strain differences in Enterococcus ,we should strengthen the monitoring of clini‐cal bacteria ,Linezolid、vancomycin、Ti Koa Laing、tigecycline preferred treatment of Enterococcus infections .

Objective To investigate changes in plasma levels of D-dimer in patients with prostate hyperplasia(BPH)complicat-ed with acute urinary retention(AUR),and to explore its clinical significance.Methods 102 cases of patients with prostate hyper-plasia complicated with acute urinary retention,treated in this hospital from July 2013 to December 2014,were selected and divided into the simple BPH group(56 cases)and BPH complicated with AUR(BPH+AUR)group.The plasma levels of D-dimer of these patients were detected and comparatively analysed.Results Compared with the simple BPH group,the plasma levels of D-dimer of patients in the BPH+AUR group was increased,had statistically significant difference.(P <0.01).Conclusion The plasma levels of D-dimer in patients with BPH complicated with AUR increase significantly,which indicates that patients are with secondary in-creased fibrinolytic activity.Measures should be taken to relieve urinary retention as soon as possible,so as to protect the vascular endothelial function of patients and reduce the incidence of complications.

Objective To Identify B-cell epitopes on monkey B virus envelope protein gD.Methods Base on bioinformatics software, secondary structure, hydrophilicity, surface accessibility, antigenic index and flexibility of monkey B virus envelope protein gD was analysed and some potential peptide epitopes were forecasted.Then, the interactions between synthetic peptides and BV positive sera were detected by Enzyme-linked immunosorbent assay ( ELISA) .At last, The sensitivity and specificity of synthetic peptides was evaluated used 20 samples of Standard sera by ELISA.Result Seven epitopes were forecasted by Bioinformatics analysis. Four synthetic peptides, sequence as 46 LPPLEQKTD54 , 106 RGAPEATRSDA116 , 291 PELAPEERGTSRTPGD306 and 361 AVYLVRRRGR370 could be reacted with positive sera pool.The sensitivity of 4 synthetic peptides changed form 40% to 70% and the specificity were 100% for 4 synthetic peptides. Conclusion There are at least four linear epitope on B virus gD protein.

Objective To explore the correlation between illness conception, health seeking behavior and mental health status in recruits. Methods 865 recruits were evaluated by Symptom Checklist 90 (SCL-90), Self-Rating Anxiety Scale (SAS), Self-Rating Depression Scale (SDS) and Self-Rating Scale of Illness Conception and Health Seeking Behavior (SSICHSB) . Results (1) Except interpersonal sensitivity, depression and hostility, there were significantly differences in other factors of SCL-90 between recruit and the national norm. Somatization and anxiety scres were significantly higher ghan the military norm. The scores of SAS and SDS were significantly higher for recruits compared to national norm. (2) There was negative correlation between the total score, each factor score of SCL-90, the total score of SDS, the total score of SAS and SSICHSB; (3) Stepwise lines regression found three factors including anxiety, the total score of SAS and obsessiveness statistically significant, when the dependent variable was the total score of SSICHSB. Conclusion There are obvious somatization,anxiety and depression in recruits. The more obvious the symptoms are, the more passive the illness conception and health seeking behavior are.

Objective:To observe the inhibiting effects of manual acupuncture(MA)on bladder cell apoptosis in rats with diabetic neurogenic bladder(DNB)based on the protein and mRNA expression of B-cell lymphoma-leukemia(Bcl)-2,Bcl-2-associated X(Bax)protein,caspase-3,and the protein expression of α-smooth muscle actin(α-SMA),transforming growth factor(TGF)-β in the bladder tissue. Methods:A DNB rat model was established via intraperitoneal injection of streptozotocin(STZ).The rats were randomly divided into a control group,a model group,and an MA group,with 10 rats in each group.For the MA group,MA was applied after modeling.The body mass,fasting blood glucose(FBG),bladder wet weight,and bladder histomorphology were observed.Protein and mRNA expression levels of Bcl-2,Bax,and caspase-3 and the protein expression of α-SMA and TGF-β in the bladder tissue were determined.The apoptotic index of bladder cells was also evaluated. Results:After STZ injection,compared with the control group,the model group and the MA group both showed higher FBG from week 3 and lower body mass from week 9(P<0.05),and had a larger bladder wet weight(P<0.05).Compared with the model group,the MA group showed a smaller bladder wet weight(P<0.05).The histopathological evaluation indicated that MA improved muscle fiber alignment and detrusor cell compensatory hypertrophy in the bladder tissue.In addition,compared with the control group,the apoptotic index increased significantly in the model group and the MA group(P<0.05);the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-β in the bladder tissue in the model group and the MA group increased significantly(P<0.05),while the protein and mRNA expression levels of Bcl-2 and the protein expression level of α-SMA in the bladder tissue decreased significantly(P<0.05).Compared with the model group,the apoptotic index of the MA group decreased significantly(P<0.05);the protein and mRNA expression levels of Bax and caspase-3 and the protein expression level of TGF-β in the bladder tissue decreased significantly(P<0.05),while the protein and mRNA expression levels of Bcl-2 and the protein expression level of α-SMA in the bladder tissue increased significantly(P<0.05). Conclusion:MA can protect the bladder by inhibiting the excessive apoptosis of bladder cells,which may be related to the down-regulation of Bax and caspase-3 proteins and mRNAs and TGF-β protein expression,and the up-regulation of Bcl-2 protein and mRNA and α-SMA protein expression.

Objective To study the uncertainty and traceability of HBV DNA assays and discuss the comparability of results among different detection systems. Methods Different detecting systems were used to detect HBV DNA using the national standard substance as "quality control substance". The uncertainty of the results was evaluated referring "Guidelines for estimating and reporting measurement uncerTAinty of chemical test results" of NATA The results were traced back to the national standard substance. According to the CLSI document EP9-A2, the results were analyzed and subjected to bias estimation with the t(0.05sv) √u2b1+ u2b2 as the criterion clinically accepted to investigate the comparability of different detecting systems. Results The means (-y) measured by 3 HBV DNA assay systems were 6.15,5.88,and 6.31 lg(kIU/L) respectively. Except system A,both the biases of system B and C had statistical significance (all P ＜ 0. 05) and expanded uncertainty of three detection systems was varied, but the difference was within the maximum acceptable range (± 0. 5) of the external quality assessment by National Center for Clinical Laboratory. Being traceable to national standard substance, the results of HBV DNA of the three detecting systems were (5.45 ± 1.23), (5.55 ± 1.32) and (5.42 ± 1.25) lg(kIU/L), respectively.There was significant difference among three systems (F = 5.63, P ＜ 0. 05). Comparing system A and B,there was significant difference in statistic (q = 5. 12, P ＜ 0. 05) and the difference between system B and C also had statistically significant (q = 6. 85, P ＜ 0. 05), but the results between system A and C had no statistical difference (q = 1.85,P ＞ 0. 05). Among these three systems, the difference of any two detection systems had no statistical significance (all P ＞ 0. 05). It showed that system bias was acceptable in clinical application and the results between different systems were comparable. Conclusions It is necessary to estimate the uncertainty and traceability when comparing the HBV DNA assay among the different labs. It also needs to estimate the bias of different systems and evaluate the clinical acceptability to ensure the accuracy and comparability of the results.

Objective:To observe the effects of Zhulian stimulant type Ⅰ acupuncture on the expression of brain-derived neurotrophic factor (BDNF) and its receptor TrkB and tissue homogenate cyclic adenosine phosphate (cAMP) in rats with diabetic bladder (DCP); To explore the mechanism of Zhulian stimulant type Ⅰacupuncture on DCP.Methods:Totally 50 SD rats were divided into control group, model group, Western medicine group, ordinary acupuncture group, Zhulian stimulant type Ⅰ acupuncture treatment group (acupuncture treatment group) according to random number table method, with 10 rats in each group. DCP rat model was prepared by intraperitoneal injection of streptozotocin (STZ), except for the control group. The Western medicine group was given mecobalamine for gavage; acupoints of "Zhongji", "Sanyinjiao", "Liechou" and "Taichong" were selected. The ordinary acupuncture group was treated with ordinary acupuncture technique, and the acupuncture treatment group was treated with Zhulian stimulant type Ⅰ acupuncture, 1 time/d, 30 minutes/time. Samples were taken after 4 weeks of treatment. The maximum bladder volume, residual urine volume and wet weight of the bladder were detected. The morphology of rat bladder was observed by HE staining. The expression level of BDNF was detected by immunohistochemistry. The expression of cAMP was detected by Western blot. The level of TrkB was determined by ELISA. Real-time fluorescence quantitative PCR (RT-PCR) was used to detect the mRNA expressions of BDNF and cAMP.Results:Compared with model group, maximum bladder volume, residual urine volume and wet weight of bladder in Western medicine group, ordinary acupuncture group and acupuncture treatment group decreased ( P<0.01), and those in Western medicine group and acupuncture treatment group were lower than those in ordinary acupuncture group ( P<0.01). The expressions of BDNF mRNA and protein, cAMP mRNA and protein in Western medicine group, ordinary acupuncture group and acupuncture treatment group increased ( P<0.05), and the level of TrkB increased, and the Western medicine group and acupuncture treatment group were higher than that in ordinary acupuncture group ( P<0.05). Conclusions:Zhuliping stimulant type Ⅰ acupuncture has a protective effect on the bladder function of diabetic rats. The mechanism may be related to the up-regulation of BDNF and mRNA, TrkB, cAMP and mRNA expressions.

Ankylosing spondylitis is an autoimmune disease with sacroiliac arthritis and spinal arthritis as the main manifestations.The disease mainly occurs in young men,has a high disability rate,and is a serious threat to the life and health of patients.Biological agents are expensive,and many adverse reactions to hormones,non-steroidal anti-inflammatory drugs,and anti-rheumatic drugs have been recorded.Traditional Chinese medicine can regulate the immunity and anti-inflammatory effects of the disease,and has good clinical effects.To promote the further study of the pathogenesis of ankylosing spondylitis and the development and screening of therapeutic drugs and therapies,in this paper,we summarize the method and mechanisms of modeling of the existing animal model of ankylosing spondylitis and analyze the advantages and disadvantages of the model.To evaluate the agreements between Chinese and Western medicine clinical characteristics,we compare the characteristics of Chinese and Western medical syndromes of the animal model.Building an animal model of ankylosing spondylitis with a higher degree of consistency between traditional Chinese and Western medicine is the key to innovative research into traditional Chinese medicine method of treating ankylosing spondylitis.To lay the foundation for research into traditional Chinese medicines and acupuncture for ankylosing spondylitis,this paper analyzes the degree of concurrence between the Chinese and Western medicine clinical characteristics of animal models.

Objective To explore the acupoint selection rules in the treatment of perimenopausal syndrome(PMS)with acupuncture by using data mining technology.Methods The clinical research literature on acupuncture treatment of PMS was retrieved from CNKI,Wanfang Data,VIP,CBM,Cochrane Library,Embase,PubMed and ScienceDirect from the establishment of the databases to August 16,2023.Excel 2021 was used to establish a prescription database for acupuncture treatment of PMS,and frequency statistics and classification of acupoints were conducted.SPSS Modeler 18.0 and SPSS Statistics 26.0 software were used to conduct association rules,clustering analysis and decision tree analysis,to explore the characteristics and compatibility rules of acupoint selection in acupuncture and moxibustion treatment of PMS.Results Totally 114 articles were included,including 115 acupoint prescriptions,and 108 acupoints with a total frequency of 787.The high-frequency acupoints were Sanyinjiao,Guanyuan,Shenshu,etc.The commonly used meridians were the bladder meridian and Conception Vessel.The majority specific acupoints were intersection acupoints,wu-shu points and back-shu points.The involved acupoints were mostly located in the lower limbs,abdomen and back.Acupuncture techniques often used the method of flat reinforcement and reduction,with the most commonly used waveform of electroacupuncture being the dilatational wave and the duration of needle retention being 10-30 minutes.The decision tree selected three simplified selection paths based on the dependent variable of Guanyuan.The core acupoints group was analyzed,and 14 groups of association rules and 7 effective cluster groups were obtained.Conclusion Acupuncture treatment of PMS follows the therapeutic principles of tonifying the kidney and harmonizing yin and yang.The acupoint combinations and core acupoint groups obtained from data mining can provide reference for clinical and scientific research.

Objective To explore the clinical acupoint selection law of acupuncture in the treatment of chronic prostatitis/chronic pelvic pain syndrome(CP/CPPS)by data mining.Methods The clinical research literature on acupuncture treatment of CP/CPPS was retrieved from CNKI,VIP,Wanfang Data,China Biology Medicine Database,Embase,PubMed,Cochrane Library and Web of Science from the establishment of the databases to November 17,2023.The prescription database was established using Excel 2021 to perform frequency statistics of acupoints.SPSS Modeler 18.0 and SPSS Statistics 26.0 were used to conduct association rules,factor analysis and clustering analysis,to explore the characteristics and acupoint selection and compatibility law in acupuncture treatment of CP/CPPS.Results A total of 105 articles was included,and 105 acupoint prescriptions were extracted,involving a total of 75 acupoints,with a total frequency of 611 times.The high-frequency acupoints were Zhongji,Sanyinjiao,Guanyuan,etc.The commonly used meridians were Conception Vessel and the bladder meridian.Jiaohui acupoints,Mu acupoints and Wushu acupoints accounted for the vast majority.The involved acupoints were mostly located in the abdomen,lower limbs,and lumbosacral region.The core acupoints was analyzed,and 19 groups of association rules,8 factors and 8 effective cluster groups were obtained.Conclusion Acupuncture treatment of CP/CPPS follows the therapeutic principles of tonifying the kidney,removing dampness,soothing the liver and removing blood stasis.The core acupoints group is Guanyuan-Zhongji-Sanyinjiao,with differentiation of syndromes and compatibility with other acupoints.