Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Female ; Humans ; Leukopenia ; therapy ; Male ; Middle Aged ; Young Adult

The embryo development technique in Zebrafish, Brachydanio rerio, is a toxicity testing method making use of the high sensitivity of fish embryo development in early stage to study and evaluate the specific effecting mechanism, the most sensitive effecting time, embryo toxicity and teratogenicity of chemicals through observing the development process of zebrafish embryo after chemical exposure to fertilized ova. This technique has been widely used to test toxicity of chemicals with the advantages of low cost, high sensitivity, simple to operate and simultaneous to detect multi-endpoints. The main methodology, technical characteristics and the status of world-wide application of this technique are reviewed in this paper. Based on the urgent environmental problems in China, the prospects to use this method for monitoring toxicity of mixed pollutants in wastewater are put forward.

OBJECTIVE:To prepare the inclusion complex capsules of actarit-HP-?-CD and investigate their dissolution rate.METHODS:The inclusion complex was prepared by the stirring method with its dissolution rate investigated.The inclusion complex capsules were prepared with fillers consisted of starch,microcrystalline cellulose,lactose and calcium sulphate.The dissolution rate of the capsules was investigated by basket-stirring method and compared with those of the pure material and the physical mixture.RESULTS:Compared with pure material and physical mixture,the inclusion complex had significant lower value of Td(P

Acupuncture Therapy ; history ; methods ; China ; History, Ancient ; Humans ; Medicine in Literature

Objective To study the clinical features of invasive pulmonary aspergillosis (IPA) with chronic obstructive pulmonary diseases (COPD),so as to provide evidence for early diagnosis and treatment.Methods A retrospective analysis was made upon clinical data,diagnosis,treatment and prognosis of 53 patients with IPA and COPD admitted between January 2005 and February 2011 collected in a respiratory unit of the Second Xiangya Hospital Affiliated Central South University.Results There were 53 cases of diagnosed as IPA with COPD,with history of using broad-spectrum antibiotics.And there were 43 cases using steroids more than 2 weeks,51 with obvious breathlessness,and 20 with fever.Early stage didn't present characteristical changes on CT scan.However,after disease progression,32 cases had maculas shadows and nonspecific consolidations in bilateral lung,14 with solitary or multiple nodules,4 with solitary or multiple air crescent sign,and 2 with halo sign.Four patients of COPD with IPA underwent bronchoscopy examination.In fungi pathogeny,sputum positive rate and galactomannan positive rate were 56.6％ and 52.8％,respectively.A total of 53 cases received antifungal treatment.Among 37 cases which underwent mechanical ventilation,24 received noninvasive ventilation and 13 received invasive ventilation.There were 33 cases which were improved and cured,and 20 cases which had no relief after half-a-month treatment or withdrew treatment.Among them,13 cases died because of multiple-organ failure (5/15) or acute renal failure (8/15).Conclusions Early suspected diagnosis,timely examination in allusion to IPA,actively searching for etiological and imaging evidences,early established diagnosis and antifungal treatment would improve prognosis of patients with COPD combined IPA who have history of high doses of cortieosteroids,obvious breathlessness and non-response to antibiotics.

Objective To evaluate the effect of repetitive transcranial magnetic stimulation (rTMS) on unilateral spatial neglect in stroke patients. Methods Published articles from the earliest date available to July, 2016 were recalled from PubMed, OVID, Embase, Co-chrane Library, CNKI, VIP and Wanfang Database. Two reviewers selected independently the randomized controlled trials (RCTs) about rT-MS for stroke patients with unilateral spatial neglect, and extracted data independently and analyzed with RevMan 5.3 and GRADE profiler 3.6. Results Twelve trials that represented 353 participants were evaluated. For improving Line Bisection Test, Star Cancellation Test, Albert Test, rTMS groups were significantly more effective than the controls. rTMS groups with different frequencies all showed significantly effec-tive:for low frequency rTMS, SMD=-1.21, 95%CI=-2.17 to-0.25;for high frequency rTMS, SMD=-2.56, 95%CI=-3.54 to-1.58;for continuous theta burst stimulation (cTBS), SMD=-2.51, 95%CI=-3.66 to-1.36. Conclusion rTMS is effective on unilateral spatial neglec-tin in stroke patients.

Objective To compare coagulation data measured with domestic produced and imported coagulation testing solutions on SD rat and human by testing prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time(TT), fibrinogen(FIB).Methods Blood samples were obtained from SPF SD rat and human .Domestic produced and imported coagulation testing solutions were applied to test PT , APTT, TT, and FIB.Results Compared to rat data measured with imported coagulation testing solution , data measured with domestic produced coagulation testing solution of PT, APTT, FIB was significantly higher (P<0.05), while, data of TT was statistically lower(P<0.05), and there was no obvious difference in human blood coagulation .Conclusion The data measured with different coagulation testing solution varies on SD rat , so the laboratories are required to establish reference data according to different products .

Objective To investigate the clinical value of serum Haptoglobin (Hp)levels in cervical cancer patients.Methods Serum Hp (with immunoturbidimetry method),SCC,CA125 (with electrochemiluminescence immunoassay method)level were detected in 45 patients with cervical cancer,37patients with cervical benign disease and 41 female healthy controls.Re-sults Serum Hp level of cervical cancer patients (mean:1.69 g/L)was strongly higher than the level of cervical benign dis-ease patients (mean:0.90g/L)and healthy subjects (mean:0.69 g/L)(t=3.26,4.42,P=0.001,0.000,respectively).Hp level rises highly in the early stage of cervical cancer.The area under receiver operating characteristic curve (AUCROC)of Hp was 0.853 which was higher than SCC (AUC=0.751)and CA125 (AUC=0.494).The sensitivity of Hp,SCC,and CA125 were 87%,49% and 29% respectively.The specificity of Hp,SCC and CA125 were 68%,88% and 83% respective-ly.Three tumor markers combined together could produce a positive detection rate of 93%.Conclusion The detection of Hp has clinic significance on diagnosis of cervical cancer,and it’s possible to become a serum tumor marker.

Objective To study the effects of injection of brain waking-liquid on unconsciouness rat as its outer brain injured. Methods To inject via abdomen the liquid to the testing rats with symptoms. Results The surviving rate and the consciousness regaining rate of every testing group is obviously higher than that of control groups. They have notable differences statistically.Conclusion The brain waking liquid is effective for curing the unconsciouness by outer brain injury.