BACKGROUND: Vertebrobasilar transient ischemic vertigo (VBTIV) is the signal of cerebral apoplexy, which is mostly contributed to arteriosclerosis and can be the pre-symptom of cerebral infarction. 1/3 cases will suffer from cerebral infarction with its natural development in several years.Specially, it is the critical stage in 1-6 months after the 1st transient ischemic attack, for which, early treatment is especially important for it.OBJECTIVE: To observe the effects of time treatment on VBTIV and its regulation on blood lipid, lipoprotein and apolipoprotein (Apo).DESIGN: Complete randomized groups and controlled comparison.SETTING: Department of Traditional Chinese Medicine in Medical College of Qingdao University.METHODS:① Totally 66 inpatients of VBTIV were selected from Department of Traditional Chinese Medical of Songshan Hospital Affiliated to Medical College of Qingdao University from January 2003 to June 2004, of which, 47 cases were male and 19 cases female. All of the patients were in the known of the observation. Two groups were randomized, named timingment group,at certain time division(7:00 am-9:00 am),warming acupuncture was applied on Zusanli (ST 36), once everyday, needle retaining for 20 minutes each time. Besides, gegen tongluo tang was oral-administrated warmly in combination, one dose each day at the specific time division, 10 days made 1 course of treatment. In the control, except at the time division, the treatment was given at any time with the same method as the timing-treatment group. Before and after treatment, enzymatic method was used to assay total cholesterol (TC), triglyceride (TG), Apo-A1 and Apo-B100; the precipitation was applied to assay high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and very low density lipoprotein cholesterol (VLDL-C). The therapeutic effects were evaluated at the end of 3-course treatment.③ χ2 and t tests were applied for the difference comparison of counting and measurement data.MAIN OUTCOME MEASURES:① Change of blood lipid before and after treatment in two groups.② Comparison of therapeutic effects after treatment between two groups.RESULTS:Totally 66 cases of VBTIV were all in result analysis.① At the end of 3-course treatment, the rate of significant improvement in timing-treatment group was higher remarkably than the control [79% (30/38),43％ (12/28), P ＜ 0.01].② Compared with the results before treatment and those after treatment in the control, the results of TC, TG, LDL-C,VLDL-C, Apo-B100 were lower remarkably (P ＜ 0.05-0.01) and the results of HDL-C and Apo-A1 were higher remarkably (P ＜ 0.05-0.01) after treatment in timing-treatment group. In the control, compared with the results before treatment, the results of TC, TG, LDL-C and Apo-B100 were lower remarkably (P ＜ 0.05-0.01) and the result of HDL-C was higher remarkably:CONCLUSION: The therapeutic effects of timing acupuncture in combination with medication are advantageous significantly compared with routine treatment on VBTIV and timing treatment reduces blood lipid much more effectively.

Objective: To observe the therapeutic effect of treatment at selected time on acute ischemic apoplexy and changes of hemorheologic indexes. Methods: 58 cases of acute ischemic apoplexy were randomly divided into a group of treatment at selected time (observation group) and a group of treatment at any time (control group). The observation group were treated by moxibustion at Zusanli (ST 36)and Shenque (CV 8)at Chen hours (7-9 o'clock am)each day, 20min each session, in combination with Chinese drugs Tongluo Decoction, one dose daily, oral administration, once at Chen hours. The control group were treated by the same methods at any hours except Chen hours. The therapeutic effects were assessed after 3 treatment courses. Hemorheologic indexes were determined before and after treatment in both groups. Results: The cured rate and the markedly effective rate in the observation group were better than those in the control group (P

188Re-HEDP is a bisphosphonate which preferentially incorporates into the sites of bone metastases.It interacts with bone metabolism,suppresses the activity of osteolysis,and gathers in tumor bone metastases.Recently,researches show that 188Re-HEDP has some advantages for palliation of bone metastasisrelated pain because of its favorable physicochemical and biological characteristics,188Re-HEDP radioisotopes therapy will be an effective method for bone metastatic tumors.

[Summary] With an increasing higher failure rate from 50%to 70%after simple sutured surgery for rotator cuff tears , scholars have put forward the patch augmented biomechanics for healing massive tears .Research hotspots lie in the material structural and physiological properties .There are two types of rotator cuff patches , which are synthetic materials ( non-degradable and degradable type ) and biological rotator cuff patch ( autologous or allogeneic tissue , xenogeneic material ) .The synthetic materials rotator cuff patch is able to provide powerful biomechanics but has severe immune rejection , and the biomaterials patch provides excellent histological reconstruction in rotator cuff and accurate regulation of biological activity of metabolic degradation but lower biomechanical maximum tensile strength .This article reviewed the current common problems and progress in the application of the rotator cuff patches .

Objective To analyze quantitatively the effects of lung inflation on the relation of airway dimensions with pulmonary function in patients with COPD.Methods 73 patients with COPD were participated in this research.All subjects would undergo CT scan when in expiration and be evaluated for the lung emphysema index(or LAA%,the percent of total lung area occupied by low at-tenuation area)and airway dimensions,including mean wall thickness (WT),the ratio of mean wall thickness with airway dimensions (WT/AD)as well as the percent of wall area (WA%)in four subsegmental bronchi(right B1,right B10,left B1,left B10).Then,all participants were divided into two groups on the basis of LAA%=1 5%,which were the mild emphysema group (n=34)and the se-vere (n=39),to compare the differences on the correlation of forced expiratory volume in 1 second (FEV1 %)with WA%,WT/AD and WT in both groups.Results There was no correlation of FEV1 % with airway dimensions in the severe emphysema group and WT in the mild group in RB10 (P >0.05).The airway dimensions of the remaining subsegmental bronchi in the mild group had a negatively relationship with FEV1 % (r=-0.382 to -0.799,P <0.05),all of which were better than the severe group (r=-0.371 to -0.687,P <0.05).Except for RB10,the remaining three indexes of airway dimensions were negatively correlated with FEV1 %, especially in RB1.For all subsegmental bronchi,the correlation between airway dimensions and FEV1 % in mild emphysema group were better than the other.Conclusion The effects of degrees in lung inflation on airway dimensions and bronchi obstruction should be taken this interaction into account during CT scan in COPD.

OBJECTIVE:To study the curative effects of Heart nourishing Granula on viral myocarditis(VMC)model mice.METHODS:The mice were randomly divided into treatment group and control group after intraperitoneal injected(inoculation)with a diluted solution of Coxsackie virus B 3 ,the general state and the mortality of which were observed.The blood sample and cardiac tissues of the mice were taken at different time after inoculated with virus for enzyme labeled com?pound assay,virus isolation test,light microscope test and transmission electron microscope test.RESULTS:The survival rate of the treatment group was higher than that of the control group.There was significant difference(P

Hereditary diffuse gastric cancer (HDGC) is an autosomal dominant inherited disease,and may be related to the mutation of CDH1 or CTNNA1 genes.Microscopically,signet-ring cell carcinoma is suggested frequently in endoscopic biopsy or gastrectomy specimens.Some patients may have concomitant extra-stomach tumor (frequently breast cancer in females).Detection of CDH1 gene mutation should be performed in high-risk individuals,and diagnosis and treatment should be carried out by a multidisciplinary team.Prophylactic gastrectomy is recommended for those with pathogenic CDH1 mutation.Endoscopic surveillance is an option for those with CDH1 mutation of undetermined significance and those without germline CDH1 mutation.This review discussed the concept,genetic characteristics,clinicopathological features and genetic screening of HDGC for providing a reference for clinicians.

Cholangiocarcinoma is a type of malignant tumor with high destruction.Due to its low diagnostic rate and high fatality rate,the operation is the unique therapeutic methods for the radical cure.However,the diagnosis and treatment for the disease were always in the phase of progression,so currently,the radical therapeutic rate is quite low,while the recurrence rate of the operation is extremely high.If the correlated mechanism of perineural invasion of cholangiocarcinoma could be understood,then interrupted its perineural invasion in the early period,that could greatly enhance the prognosis of cholangiocarcinoma patients.This article systematically reviews the progress of cholangiocarcinoma neural invasion related molecules and possible mechanism.

Influenza A virus is an enveloped virus that belongs to the Orthomyxoviridae family. It has 8 negative RNA segments that encode 16 viral proteins. The viral polymerase consists of 3 proteins (PB 1, PB2 and PA) which plays an important role in the transcription and replication of the influenza A virus. Polymerase basic protein 1 (PB 1) is a critical member of viral polymerase complex. In order to further study the function of PB1, we need to prepare the PB1 antibody with good quality. Therefore, we amplified PB1 conserved region (nt1648-2265) by PCR and cloned it into pET-30a vector, and transformed into Escherichia coli BL2 1. The expression of His tagged PB 1 protein was induced by IPTG, and His-PB 1 proteins were purified by Ni-NTA resin. For preparation of PB 1 protein antiserum, rabbits were immunized with His-PB 1 fusion protein 3 times. Then the titer of PB 1 polyclonal antibody was measured by indirect ELISA. The antibody was purified by membrane affinity purification and subjected to immunoblotting analysis. Data showed that PB1 antibody can recognize PB 1 protein from WSN virus infected or pCMV FLAG-PB 1 transfected cells. Meanwhile, PB 1 antibody can also recognize specifically other subtype strains of influenza A virus such as H9N2 and H3N2. PB 1 polyclonal antibody we generated will be a useful tool to study the biological function of PB1.
Animals ; Antibodies, Viral ; biosynthesis ; Cloning, Molecular ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli ; metabolism ; Genetic Vectors ; Influenza A Virus, H3N2 Subtype ; Influenza A Virus, H9N2 Subtype ; Plasmids ; Rabbits ; Viral Proteins ; immunology

BACKGROUND:The hair fol icle stem cells are usual cultured in Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum. Recently, keratinocyte serum-free medium has been used to culture hair fol icle stem cells.
OBJECTIVE:To observe the effects of three different culture media on the proliferation and purity of hair fol icle stem cells.
METHODS:After sterling, the skin of the rat’s beard was cut off, and the vibrissa fol icles were dissected under a stereocroscope. The fol icles were digested by Dispase Ⅱ firstly and by the mixture of trypsin and EDTA secondly. cellsuspension was divided into three pieces based on the average number of cells and respectively cultured by keratinocyte serum-free medium, Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 containing 10%fetal bovine serum and keratinocyte serum-free medium containing 10%fetal bovine serum. Hair fol icle stem cells were selected by rapid adherence on col agen type Ⅳ, and passaged.
RESULTS AND CONCLUSION:Cultured hair fol icle stem cells spread to the third generation, and trypan blue assay showed there was no difference in cellsurvival rate among the three groups (P>0.05). cellCounting Kit 8 assay showed that the cells grew slowly in the three groups in the first 2 days. At day 4, the hair fol icle stem cells grew into the growth logarithmic phase, and the proliferative activity was ranked as fol ows:keratinocyte serum-free medium+10%fetal bovine serum>Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum>keratinocyte serum-free medium with a significant difference between the three groups (P<0.05). Flow cytometric examination showed the expression of CD34 in the keratinocyte serum-free medium+10%fetal bovine serum group was lower than that in the keratinocyte serum free medium group (P<0.05). The expressions of CD34, CK15 andβ1-integrin (CD29) in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum were lower than those in the other two groups (P<0.05). These findings indicate that we can obtain higher purity hair fol icle stem cells in the keratinocyte serum-free medium+10%fetal bovine serum than in the Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12+10%fetal bovine serum.