Objective To investigate the level of serum cancer antigen 125 (CA125) and lactic dehydrogenase(LDH) and clinical features of patients with non-Hodgkin lymphoma(NHL), and the correlation between the level of serum CA125 and LDH. Methods Forty patients with NHL were studied and the variances of the serum CA125, LDH level before treatment were analysed. Results A higher serum of CA125was associated with clinical phase,group,hydrothorax and ascites,intemational prognostic index (IPI), and had weaker positive correlation to LDH. Conclusion Measurement of serum CA125 had important clinical significance to the patients with NHL especially to the patients with hydrothorax and ascites.

Objective To investigate the killing effect of allitride on fungus inside Candida albicans biofilm (BF) .Methods The polyascchride components of BF was labeled by FITC-conA .The fluorescence microscopic was adopted to observe the effect of al-litride on the BF forming process of Candida albicans in morphology .The specimens were divided into 3 groups ,the control group (allicin concentration of 0) ,0 .25 mg/mL allicin group ,0 .5 mg/mL allicin group ,1 .0 mg/mL allicin group ,2 .0 mg/mL allicin group .The XTT reduction assay was performed to quantitatively detect the killing effect of allitride on fungus inside Candida albi-cans BF .Results After the treatment by different concentrations of allitride ,the BF structure was observed by fluorescence micros-copy .The fluorescence intensity of high concentration group was weakened compared with the low concentration group and the con-trol group .The ODA450 values of the XTT reduction assay after acting on Candida albicans BF for 5 h by different concentrations of allitride (0 .5 ,1 .0 ,2 .0 mg/mL) were 1 .87 ± 0 .31 ,1 .64 ± 0 .25 and 1 .30 ± 0 .29 ,respectively ,and the OD450 value of the control group was 2 .11 ± 0 .26 ,there was statistically significant difference between the allitride groups and control group (P<0 .05);after acting on Candida albicans BF by 0 .25 mg/mL allitride ,the OD450 value was 1 .93 ± 0 .31 ,the difference had no statistical signifi-cance compared with the control group(P>0 .05) .Conclusion Allitride can kill the fungus inside BF of Candida albicans and has the inhibiting effect on biofilm .Moreover the inhibiting effect on biofilm is increased with its concentration increase within certain concentration range .

Tunneling nanotubes(TNT) are cellular communication and transport mechanism discovered recently. TNTs play important role in immunological response and viral spread, also may involve embryonic development, regulation of gene expression and carcinogenesis. However, it was not easy to study TNT for clinical samples since intricate assay method for TNT. We suggested using histochemical assay PAS staining in cell drip to show TNT in leukemia and lymphoma samples.

Objective To observe the efficacy of CTLA-4Ig gene local transfection on the survival of renal allografts. Methods The kidneys of BN rats were transfected with CTLA-4Ig gene-recombined adenovirus. The transfected kidneys were transplanted to Lewis rats (transfection group). BN→Lewis kidney transplantation with non-transplanted kidneys served as controls. The allograft survival time and the allograft function between the two groups were compared. Results The allograft survival time of transfection group was longer than that of controls significantly (32? 8.0?days vs 8.5 ? 1.4?days , P

Objective To explore the influential factors of dangerous placenta previa pathogenesis,and to analyze their peri natal period outcomes.Methods The clinical data in 143 pregnant women and parturients of dangerous placenta previa(research group) and 286 pregnant women and parturients of non-dangerous placenta previa(control group) in our hospital from January 2011 to December 2015 were retrospectively analyzed.Results The average age of the research group was higher than that of the control group (t =2.92,P =0.004);the previous pregnancy frequency,previous parity,previous abortion frequency,previous cesarean delivery frequency and incidence of placenta previa in the research group were higher than those in the control group(P＜0.01);the incidence of placenta previa in the research group was higher than that in the control group(x2 =7.60,P=0.006);the prenatal gestational weeks of the research group were lower than those of the control group(t=-12.33,P＜0.01).The multi-factor analysis showed that the age,previous abortion frequency and previous cesarean delivery frequency were the influence factors of placenta previa occurrence.Among them,the previous cesarean delivery frequency was the main risk factor.The occurrence rates of antepartum hemorrhage,postpartum hemorrhage,uterine removal and premature infants in the research group were higher than those in the control group(P＜0.01);The intraoperative blood loss of the research group was higher than that of the control group(P＜0.05);the neonatal birth weight and the 1 min Apgar score of the research group were lower than those in the control group(P＜0.05).Conclusion The age,previous abortion frequency and previous cesarean section frequency are related to the occurrence of dangerous placenta previa.

OBJECTIVE:To observe therapeutic efficacy and safety of low-molecular-weight-heparin-sodium combined with dy-drogesterone in the treatment of threatened abortion. METHODS:Medical information of 72 patients with threatened abortion were analyzed retrospectively and divided into control group(36 cases)and observation group(36 cases). Control group was given Dy-drogesterone tablet with initial dose of 40 mg,one day later every 12 h 10 mg/time. Observation group was additionally given ab-dominal subcutaneous injection of Low-molecular-weight-heparin-sodium injection 5000 U,once a day,on the basis of control group. Both groups were treated for a week. Clinical efficacies of 2 groups were observed,and lumbar acid,vaginal bleeding,ab-dominal pain time,total treatment time,neonatal birth weight were also observed. The levels of hs-CRP,fibrinogen,D-dimer and platelet,the occurrence of ADR were observed before and after treatment. RESULTS:The total response rate of observation group was significantly higher than that of control group(91.7%vs. 80.6%),the backache,vaginal bleeding,abdominal pain time and to-tal treatment time of observation group were significantly shorter than those of control group,the neonatal birth weight and gesta-tional age of observation group were significantly more than those of control group,with statistical significance(P<0.05). Before treatment,there was no statistical significance in the levels of hs-CRP,fibrinogen,D-dimer and platelet between 2 group(P>0.05);after treatment,the levels of hs-CRP,fibrinogen,D-dimer and platelet in 2 groups were significantly lower than before,and the observation group was significantly lower than the control group,with statistical significance (P<0.05). There was no statistical significance in the incidence of ADR between 2 groups (P>0.05). CONCLUSIONS:Based on routine treatment,low-molecu-lar-weight-heparin-sodium combined with dydrogesterone shows good therapeutic efficacy and safety for threatened abortion.

Allee effect is a phenomenon in ecology characterized by a correlation between population size or density and the mean individual fitness of population or species.It is consistent with density dependent phenomenon in biomedical field.Recently,Allee effect has been studied by theoretical biologist in detail for oncology biological research.Leukemia cells,which reside in organic microenvironment,show an obvious Allee effect.The authors compared Allee effect with density dependent growth of leukemia cells based on their work experience and literature data.No principle distinction was found between these two terms.In this paper,Allee effect in leukemia cell culture and leukemia,especially in minimal residual disease,will be discussed in the view of ecology.The association between Allee effect and leukemogenesis and relapse dynamics will also be explored in the future research.

The disturbance of life rhythm exerts negative influence on modem life.The relationship between the disruption of circadian rhythms and tumor growth has attracted much attention.It has been studied in breast cancer and prostate cancer,and chronotherapeutics has been used for treatment of some cancers in clinic.Researchers have begun to investigate the association of circadian rhythms with leukemia/lymphoma outside China.The literatures were reviewed and the launch of research on this field was appealed.

Human herpesviruses (HHV) are widespread all over the world,and persist in human in latent infection.When reactivated,HHV plays an important role in the pathogenesis and development of tumor and other diseases.Much attention has been paid on EB virus which can cause different tumors and other diseases.Studies within this half century have elucidated the mechanism of oncogenesis of γ-HHV.Recently it was reported that other HHV may have oncomodulation functions.

Cell death as the partner of cell proliferation is one of the basic mechanism in cell biology. Recently, it became obscure since more and more patterns of cell death were reported. However, the classification of cell death, which was recommended by the Nomenclature Committee on Cell Death (NCCD) in 2009, clarified the criteria. The theme of the recommendation and recent data from the literature were discussed in this review. The significance of studies on cell death was discussed.