Dioxin,as a kind of strong carcinogenic substance,a kind of environment hormone with tremendous harm,can get into human body through food chain and accumulate in fat of animals and persons,forming serious threat to human health. Dioxin has been included in the list of monitoring objects in food part of the global environment monitoring program as a new environmental pollutant by World Health Organization. In this paper,the toxicity,the source of dioxin and its impact on human health and prevention and control measures are introduced.

OBJECTIVE:To investigate the effect of liposome formulation in promoting hepatocytes' uptake of tenofovir and cytotoxic effect. METHODS: The tenofovir cationic liposomes were prepared by tert-butyl lyophilization, and its encapsulation efficiency and physico-chemical property were determined. Using human hepatic carcinoma cells SMMC-7721 as a model to investigate the effect of liposome formulation in promoting hepatocytes' uptake of tenofovir. MTT assays were used to examine the toxicity of tenofovir cationic liposomes in different conditions. RESULTS: The prepared tenofovir cationic liposomes had an encapsulation efficiency of (88.3?1.6)%, a size distribution of (278.4?67.6)nm and a Zeta potential of (31?5)mV. The results showed that the liposome formulations containing galactose and PEG modifying lipids resulted in significantly higher and prolonged drug accumulation inside cells as compared with free drugs. The cell survival rates were above 80% when the tenofovir liposome was at a concentration of 7.5?g?mL-1 and lipids at a concentration of 30?g?mL-1, and little toxicity was noted. CONCLUSION: The prepared cationic liposome can greatly enhance hepatocytes' uptake and protection of tenofovir, which is expected to become a highly effective drug delivery system of antiviral drugs such as tenofovir etc.

Objective:To explore relationship amongpersonality,family interpersonal dynamics and college students'adaption.Methods:Adaption to college was assessed with the Symptom Checklist 90 (SCL-90)in internet when 1850 freshman selected conveniently from a university in Shanghai (Students with single factor score of SCL-90 equal or greater than 2.5 belonged to the positive group,while the other students with single factor score of SCL-90 all less than 2.5 belonged to the negative group).At the same time,they were evaluated with the Cattell's 16 Personality Factor(16PF)to evaluate their characteristics and Multi-Interpersonal Dynamics Questionnaire for Un-dergraduates (MIDQ-U)for undergraduate students to assess family interpersonal dynamics.Results:The scores of anxiety of 16PFwere higherin the positive groupwith 50 students than in the negative group[(7.2 ±1.4)vs.(4.6 ± 1.9),P <0.001 ],while the scores of extroversion,serenity and alertness of 16PF were lower in the positive groupthan inthe negativegroup.In the SCL-90 positive group,the MIDQ-Uscores of indifference and submissivenesswere higher[(17.3 ±6.0)vs.(14.9 ±4.2),(11.5 ±3.2)vs.(10.3 ±3.1);P <0.001]and the scores of indepen-dencewere lower than that in the positive group[(18.7 ±3.0)vs.(19.8 ±3.4),P =0.02].Path graphanalysiss-howed that anxiety and extroversionpartly predicted freshmen's adaption directly(β=0.57 --0.01 )and partly worked through indifference(β=0.031 --0.004),while decisivenesscompletely functioned through indifference with the coefficient of 0.008.Anxiety had part mediating effect between indifference and freshmen's adaption(β=0.008).In the other three factors,anxietyplayedcompletemediatingrole.Conclusion:Personalities and family inter-personal dynamics may affect each other,then mutually predictstudents'adaption to college.

Objective To explore family functioning and related factors in anxiety disorder(AD)adoles cents.Methods 84 adolescent patients with AD and 124 controls without mental disorder matched by gender,age,and residential township were assessed with Family Adaptability and Cohesion Scale(FACES-Ⅱ),Family Assessment Device.Chinese version(FAD-CV)and demographic questionnaires.Results There were statistical differences between case and control groups in family economic conditions(P<0.05).There was lower family cohesion in AD group(64.82±9.63)than that in the control group(69.72±8.91);the scores of communication,emotional involvement and behavioral control were higher in AD group((2.34±0.45),(2.52±0.44),(2.46±0.32))than that in controls((2.12±0.34),(2.36±0.42),(2.24±0.24)),all P<0.05.Logistic regression analysis showed that better family economic condition(OR=0.784;95%CI:1.459-3.396)and higher cohesion of family(OR=0.969;95%CI:0.942～0.998)were protective factors for AD,while over-strict behavioral control (OR=2.181;95%CI:0.993～2.441)was a risk factor for AD.Conclusion There are different family functioning characters between AD and controls,and the intimacy relationship and suitable behavior control in family may improve the mental health of adolescents.

Objective To evaluate the clinical efficacy and safety of injection azithromycin in the treatment of the acute bacteria pneumonia of infant. Methods A randomized controlled open trial was carried out. Erythromycin as a controll drug was compared the clinical efficacy and safety with azithromycin as a test drug. Results In azithro-mycin group,the clinical effective rate was 90.6% ;the fully recovered rate was 75.5%. While those of erythromycin group were 68.0% ,36.0% respectively. The incidence of adverse reaction was 9.3% and 30.4% respectively in two groups. Conclusion Injection azithromycin of new macrofides type is a kind of effective and safe anti-bacterial drug.

OBJECTIVE To know the bacterial distribution in the blood of septicemia patients and the characteristics of drug resistance.METHODS To train the blood in BD BACTEC 9050 full-automatic blood training system and to analyze it in the FOUTUNE IMS bacterial identification and hypersusceptibility analysis system of Fuxing Company.RESULTS The Gram-negative bacilli were in superiority with 64.9% of the total isolated bacteria.Then were the Gram-positive cocci,and Staphylococcus were the main.The Enterococcus and fungi were with a certain proportion.The drugs to which had relatively low resistance for the Gram-positive bacilli and the Gram-positive cocci were the carbapenems,the enzyme inhibitors and vancomycin.CONCLUSIONS The Gram-negative bacilli get priority in the blood bacteria in patients with septicemia during the last two years,the enterococci and fungi have a certain proportion.And the pathogenic bacteria have higher resistance clinically to antibacterial drugs.

Aim To explore the effects of apigenin on A?_ 25-35 induced injury in primary cultured hippocampal neurons.[WTHZ]Methods[WTBZ] The primary cultured hippocampal neurons were exposed to 10 ?mol?L-1 A?_ 25-35 for 24 h after pretreatment with the apigenin of concentrations of 5,10,20,40 ?mol?L-1 for 2 h;then the survival rate of hippocampal and SOD activity were examined;we also analysed the expression of apoptosis-related gene bcl-xl with Western blot.Result The survival rate of hippocampal neurons were decreased after pretreatment with 10 and 20 ?mol?L-1 apigenin for 2 h,the increases of Mn-SOD activity and bcl-xl protein expression were also observed.Conclusion Apigenin has the bioactivity of protecting the neuron from cytoxic induced by A?_ 25-35 in cultured hipocampal neurons as estrogen.The mechanism is likely related with activating the SOD and increasing bcl-xl protein expression,which plays a pivotal role in protecting cells from apoptosis.

Objective:To study effect of tripterygium glycosides(TG)on T-lymphocyte subsets in rats with idiopathic thrombocytopenic purpura(ITP),and further to discuss the possible mechanism of TG in treating ITP.Methods:ITP animal model was established by intraperitoneal injection of exogenous anti-platelet–serum(APS).Sixty model rats were randomly divided into five groups:normal group,saline group,the high and low dosage of TG groups,prednisone group.Every rat was giving liquid medicine by intragastric administration one time per day for ten days 36 hours after the first intraperitoneal injection of exogenous APS.The changes of peripheral hemogram,the amount of megakaryocyte of bone marrow and T-lymphocyte subsets were observed.Results:In high dosage of TG group,the number of platelet count increased obviously,the number of megakaryocyte in bone marrow,CD8+and CD4+decreased,and CD4+/CD8+increased.Conclusion:High dosage of TG had obviously therapeutic effect on IPT rat,such as increasing platelet count obviously,reducing number of megakaryocyte in bone marrow obviously and ameliorating T-lymphocyte subsets.The mechanism may be related to regulating the cell immune function.

Objective To investigate the effect of electro-acupuncture at Guanyuanshu, Xinshu, Neiguan points on cardiac function after acute myocardial ischemia in rabbits. Methods A total of 36 rabbits were randomly divided into a control group, a model group and a treatment group. Acute myocardial ischemia was induced with Jugular vein injection of pituitrin. At 10 min before modeling, 1 hour and 1 week after modeling, the levels of malondialdehyde (MDA) and creatine kinase(CK) were determined respectively, the cardiac function was assessed as the maximum rate of left ventricular pressure rise(dp/dtmax)and the peak left ventricular pressure (LVSP). Results After 1 week of treatment, the serum levels of CK and MDA in the treatment group reduced significantly(120.21±10.67 U/L, 21.35±2.04 mmol/L], compared to the model group (162.37±13.21 U/L, 30.13±2.75 mmol/L) (all P<0.05);the dp/dtmax and LVSP decreased percentage in the treatment group (20%±2.43%, 7.22%±0.97%) decrease significantly compared to the model group (31.5%± 4.14%, 12.19%±2.21%) (all P<0.05). Conclusion Electro-acupuncture of Guanyuanshu, Xinshu, Neiguan acupoints can significantly improve cardiac function after acute myocardial ischemia and protect against acute myocardial ischemia in rabbits.

Objective To discuss the clinical and X-ray representations and to improve the knowledge of primary alveolar sarcoma of the bone . Methods The manifestaion of 4 patients with primary alveolar sarcoma of the bone comfirmed by operation and pathology were analyzed retrospectively and relevant literature were reviewed.Results The tumors often located in the long tubular bone and most of them were occurred at the lower limb, The main X-ray features were osteolytic and expansive bone destruction, periosteal reaction was usually absent but soft tissue mass commonly occurred. Pathological characteristics were of typical tumor cell of the aggregating to form solid hest-like or alveolar structure. Conclusion The tumors is rare, this neoplasm is lack of characteristic X-ray features and the finial diagnosis depends on pathology.