The effects of ranitidine (Ran) on cultured myocardial cells of neonatal rats were observed. Ran 100 ?mol?L-1 took no effect on spontaneous beating rates of cultured myocardial cells, but it decreased the beating rates increased by histamine 10 ?mol?L-1 and did not inhibit the beating rates increased by isoprenaline 2?mol?L-1. Histamine 10?mol?L-1 increased the amplitude, maximal rate of rise and pro-longed action potential duration at 50% (APD50) and 90% (APD90) repolarization. The effect was abolished by Ran 100?mol?L-1. The results suggested that Ran may possess antiarrhythmic action induced by histamine's increase.

Objective To investigate the comparability of test seven tumor marks results of two electrochemiluminescence immu-noassay analyzer between Roche Cobas E411 and Cobas E601 .Methods According to United States Clinical and Laboratory Stand-ards Institute (CLSI) EP9-A2 document ,E411 was acted as comparison method (X) and E601 was as the experimental method (Y ) .Alpha fetoprotein (AFP ) ,carcinoembryonic antigen (CEA ) ,carbohydrate antigen125 (CA125 ) ,carbohydrate antigen199 (CA199) ,carbohydrate antigen153(CA153) ,total prostate specific antigen(TPSA) and free prostate specific antigen(FPSA) levels of patient samples were tested and the results were analyzed .Results The correlation between the two systems was good and the bias was acceptable in the medical decision level .Conclusion Two systems have good concordance in detecting the seven tumor markers ,w hich can meet the clinical need .

Objective To investigate the therapeutic effect of Ganglioside(GM1) on acute cerebral infarction.Methods 60 cases with acute cerebral infarction were divided into two groups randomly.At the basic of routin treatment for the twe gyoups,GM1 group was treated with GM1 40 mg/d for 14 days and control group was treated with Citicoline 0.75 g/d for 14 days.The neurological deficit scores(NDS),activities of daily living(ADL) and adverse reactions were evaluated.Results After treatment,the scores of NDS and ADL were singnificantly decreased in twe groups than before treatment((P

Objective To compare the characteristics of digital subtraction angiography ( DSA) and compu-ted tomography angiography ( CTA) of moyamoya disease .Methods To analyze the characteristic of DSA and CTA in 29 cases with moyamoya disease ,including arterial occlusion ,arteriarctia,abnormal proliferation of vascular ,collat-eral circulation.Results There were no statistically significant differences between DSA and CTA in detecting arteri-al occlusion,arteriarctia(CTA 53 hemicerebrum,DSA 57 hemicerebrum)(χ2 =2.167,P>0.05),abnormal prolifera-tion of vascular (abnormal:CTA 41 hemicerebrum,DSA 45 hemicerebrum;normal:CTA 16 hemicerebrum,DSA 12 hemicerebrum)(χ2 =0.757,P>0.05).But there was statistically significant difference between DSA and CTA in detecting collateral circulation .Conclusion CTA is a good method to find out moyamoya disease .But DSA is better in discovery collateral circulation of moyamoya disease .

Aim To demonstrate the effects and mechanism of adenosine A1 receptor agonist R(-)-N6-(2-phenylisopropyl) adenosine(R-PIA) on high glucose(HG)-induced myocardial hypertrophy by in vitro cultured myocardial cells from neonatal rats.Methods The protein content was assayed by the method of Lowry. The expression of p-ERK1/2 and ERK1/2 was determined by Western blot.The [Ca~(2+)]I transient changes of cell loaded Fura-2/AM were measured by Till image system.Results 1 μmol·L~(-1) R-PIA and U0126 inhibited similarly HG-induced increase of the protein content and [Ca~(2+)]I transient along with the relative expression of p-ERK1/2.These responses were completely abolished by adenosine A1 receptor antagonist 8-cyclopentyl-1,3-dipropylxanthine(CPDPX).Conclusion Adenosine A1 receptor stimulation significantly inhibits HG-induced myocardial hypertrophy by mediating ERK1/2 pathway and Ca~(2+).

Objective To observe the effects of Coptidis Rhizoma on gastrointestinal motility functions, MTL and GAS in spleen-deficiency rats; To explore the dose-effect mechanism of “invigorating stomach” and “spoiling appetite”.Methods Fatigue and troublesome were used to establish exhausted spleen-deficiency models. 60 SD rats were randomly divided into blank control group, model group, positive medicine group, Coptidis Rhizoma high-, medium- and low-dose groups, with 10 rats in each group. Each medication group received relevant medicine for gavage, while the blank control group and model group received normal saline for gavage, once a day for 14 d. The general gastrointestinal motility functions, MTL and GAS of rats in each group were observed.Results Compared with blank control group, the gastric residual rate of model group was significantly higher; small intestinal propulsion rate, MTL, and GAS were significantly lower (P<0.05,P<0.01). Compared with model group, the gastric residual rate in Coptidis Rhizoma low-dose group was significantly lower; small intestinal propulsion rate, MTL and GAS increased significantly (P<0.05,P<0.01); small intestinal propulsion rate and MTL in Coptidis Rhizoma high-dose group were significantly lower (P<0.05).Conclusion High-doseCoptidis Rhizoma leads to “spoiling appetite”, and low-dose Coptidis Rhizoma leads to “invigorating stomach”, which may be related to adjusting gastrointestinal motility directly or adjusting the secretion of gastrointestinal hormones.

AIM : To explore the effects of DECB (deproteinized extract of calf blood, DECB) on hypoxia-reoxygenation-induced injury in cultured cardiac myocytes. METHODS : By using cultured myocardial cells as models, thenumber of death cells, beating frequency and the content of LDH were observed after hypoxia-reoxygenation-induced injury. RESULTS : 0.1 and 0.3 g?L -1 of DECB decreased the number of death cells and the content of lactate dehydrogenase (LDH), and restored the cellular beating frequency in the supernatant. CONCLUSION : DECB can protect the cultured myocardial cells against hypoxia-reoxygenation-induced injury.

Objective To evaluate the effect of various rewarming regimes on rectal temperature and hemodynamic parameters of dogs after open abdominal injury and seawater immersion. Method 30 healthy dogs were subjected to open abdominal injury and 2-hour seawater immersion. Then dogs were divided into three groups: group A (n=10) received no special warming treatment, in group B(n=10) electric blanket was used to warm up to 42℃, in group C (n=10) animals were warmed by 1/3 saline solution in 42℃ for 20 minutes and continuous perfusion of the abdominal cavity with 1/2 saline solution for 2 hours. The changes in body temperature, heart rate, MAP, CVP and CO were observed before and after rescue. Results The results of group A were not satisfactory. The rectal temperature, MAP, HR and CO were not recovered to normal after 6 hours of treatment. and the rectal temperature was 34.8℃ after 6 hours. The rectal temperature rose to 36.9℃ after being rewarmed with an electric blanket in group B. The result was better than that of group A. But there was tachycardia. The effects of rewarming from hypothermia in group C was satisfied. All hemodynamic parameters and rectal temperature recovered after 4 hours treatment. Conclusion Cold seawater immersion after open abdominal injury would result to hypothermia, and intra-corporeal rewarming should be the optional treatment.

Objective To evaluate the effect of seawater immersion on hemodynamics and pathological changes in dogs with perforating injury of the intestine. Methods Thirty healthy adult dogs were subjected to perforating injury of the intestine. Ten injured dogs were immersed in artificial seawater after the injury. An equal number of injured dogs were immersed in normal saline solution. Control group dogs (n=10) were not subjected to salt water immersion. Hemodynamical and pathological changes were observed at different time intervals after injury. Results Obvious hemodynamic disorder and serious pathological changes were found after seawater immersion. Normal saline solution group and control group dogs did not show obvious disorders in hemodynamics and pathology. Conclusion Seawater immersion is one of the main factors leading to the disturbance in hemodynamic and pathological changes after perforating injury of the intestine.

HERG/IKr plays an important role in repolarization of cardiac action potential. In pathological condition such as cardiac ischemia, congestive heart failure and myocardial hypertrophy, the density and kinetic properties of HERG/IKr will change. This review is about modulation of HERG/IKr in pathological condition and effect of traditional chinese medicine on HERG/IKr.