Objective To observe the clinical effect of point-to-point acupuncture with electric stimulation of direct current induction for facial nerve paralysis. Methods A total of 65 patients with facial nerve paralysis were randomly divided into the observation group (n=33) and the control group (n=32). The control group received routine treatment, and observation group was received Dicang (ST4)-to-Jiache (ST6) and Yangbai (GB14)-to-Taiyang (EX-HN5) by acupuncture with electric stimulation of direct current induction combined with conventional western medicine. The treatment of both groups last 3 weeks. The House-Brackmann Scale and the BR indexe at baseline and after 3 weeks treatment were observed. Results After treatment, The clinical effect of observation group was 66.7%, which was significantly higher than control group 43.8% (U=2.175, P=0.013). After treatment, House-Brackmann facial nerve scale (Ⅰ level=15 cases, Ⅱ level=4 cases, Ⅲ level=3 cases) in the observation group was significantly better than that in the control group (Ⅰ level=5 cases, Ⅱ level=7 cases, Ⅲ level=2 cases; U=2.069, P=0.018). After treatment, R1 latency (12.28 ± 2.55 ms vs. 13.84 ± 3.07 ms, t=2.227) and R2 latency (32.28 ± 7.08 ms vs. 35.95 ± 5.11 ms, t=2.396) in the observation group were significantly shorter than those in the control group (P=0.030, P=0.020). Conclusions The point-to-point acupuncture with electric stimulation of direct current induction could reduce latency of R1 and R2 for facial nerve paralysis, and improve the clinical effect.

BACKGROUND:Numerous studies have demonstrated that mild hypothermia has a better protective effect on neurons after cerebral infarction.
OBJECTIVE:To investigate theeffect of mild hypothermia on nerve regeneration microenvironment of infarcted area in rat models of cerebral infarction and analyze its possible effects on neural functional recovery after cerebral infarction.
METHODS:Twenty out of 65 adult femaleSprague-Dawleyrats were randomly selected as the sham group. The remaining 45 rats were subjected to carotid artery ligation to establish rat models of cerebral infarction. Five rats were rejected because of modeling failureor death, the remaining 40 rats were randomly and evenly divided into cerebralinfarction and mild hypothermia groups. The head temperature of rats in the cerebral infarction group was downregulated to (37±1)℃ using a semiconductor refrigeration instrument. The rats were transferred to the room with the temperature of 25℃ after the operation. Brain hypothermia was also induced in rats from the mild hypothermia group. At 13.0-14.0 minutes after establishing rat models of cerebral ischemia, the head on the side of cerebral ischemia was tightly connected with the probe of the semiconductor refrigeration instrument. The refrigerator temperature was adjusted to 6-8℃, so as to make the temperature of brain tissue on the lesion side at 32.0-33.0℃ for 4 hours.
RESULTS AND CONCLUSION:Compared with the cerebral infarction group, the BBB scores of rats inthe mild hypothermia group were distinctly increased, and the volume of infarcted area decreased. At 1 day after modeling, the expression level of growth associated protein 43 mRNA in brain tissue of rats in the mild hypothermia group was close to that in the cerebral infarction group. At 2 weeks after modeling, the expression level of growthassociated protein 43 mRNAin brain tissue of rats in the mild hypothermia group was significantly increased compared with that in the cerebral infarction group. These results suggest that mild hypothermia therapy can protect nerve cels against injury caused by cerebral infarction and promote the recovery of neurological function. Its underlying mechanism may be related to the up-regulation of the expression of growth associated protein 43 in ischemic penumbra .

China has entered a new economic and social development,public hospitals have to face the further deepening of the medical system reform,public welfare and welfare,and to face the fierce market competition in the field of social capital into the medical field.It is necessary to build a public hospital management accounting system.The significance of the construction of public hospital management accounting system is expounded,the problems of the construction of public hospital management accounting system are found out,and the corresponding countermeasures are put forward.

In recent years,more and more of the enzyme proteins have been carried out using recombinant microorganism bioreactor for large scale production.For reasons of improved the catalytic capability and environmental suitability,or enhanced expression level of the protein,a variety of genetic engineering technology according to protein molecule modification have been applied extensively.Major strategies and achievements of molecular modification for microbial enzyme,such as site-directed mutagenesis,error-prone PCR,DNA shuffling and optimum codon design were reviewed.

Objective:To compare the assessments of the presence or absence and the changes of post-treatment periapical lesions using periapical radiographs versus cone-beam computed tomography (CBCT).Methods:Imaging records were selected from a sample of 233 radiographic examinations (in-cluding periapical radiographs and CBCT)of patients with chronic apical periododontitis who received treatment and review in Department of Cariology and Endodontology of Peking University School of Stoma-tology.After testing the inter-observer and intra-observer agreement,two observers determined the pre-sence or absence of a periapical lesion and classified the changes of lesions in 4 categories:absence,re-duction or enlargement of lesion,or uncertain.The differences between CBCT and periapical radiographs in detecting the presence or absence and the changes of periapical lesions were compared using McNemar test and Fisher’s exact test respectively.Results:In the study,233 teeth were assessed with both periapi-cal radiographs and CBCT images.Periapical lesions were detected in 178 teeth on CBCT scans and in 146 teeth on periapical radiographs.CBCT images revealed a significantly larger number of lesions than were revealed by periapical radiographs (P <0.01 ).There was disagreement on the changes of lesions between the two radiological technologies in 25% teeth,and the difference was statistically significant (P <0.01 ). Conclusion:Significant differences were observed between periapical radiographs and CBCT in detection of post-treatment periapical lesions and assessment of the changes of periapical lesions.

Objective To investigate the contents of dioxane in shampoo and bath lotion type of cosmetics. Methods Headspace gas_chromatography was applied to determine the concentration of dioxane in cosmetics. Results Significant differences were observed between 122 national products of shampoo with a detectable rate of 63.9% and a value of P95 of dioxane concentration of 173.4 ?g/g and 64 imported and joint_venture products of shampoo with a detectable rate of 56.2% and a value of P95 of dioxane concentrations of 34.68 ?g/g (P

Objective: To compare the incidences of root cracks after canal instrumentation with HyFlex CM system and the ProTaper Universal system .Methods:Sixty mandibular incisors were mounted in resin blocks with simulated periodontal ligaments , and the apex was exposed .The control group of 20 teeth was not prepared , and the other 40 teeth were divided into 2 experimental groups ( n=20 ) .The 40 root canals of the experimental groups were instrumented using HyFlex CM and ProTaper Universal to the major apical foramen ( AF).The horizontal sections 1 mm, 2 mm, and 3 mm from the apex were observed under an optical stereomicroscope at 25 ×magnification .The presence of cracks was noted . Results:No cracks were found in the control teeth .Cracks were found in 1 of 20 ( 5%) teeth in HyFlex CM group , and 17 of 20 ( 85%) teeth in ProTaper Universal group .The difference between the two experimental groups was statistically significant (P<0.01).Conclusion: The HyFlex CM files caused fewer root cracks than the ProTaper Universal files during the root canal instrumentation .

objective To observe the effects of co-culture of hTNFα-sercreting and human colon cancer cells LOVO on the proliferation of cancer cells. Methods The stable transfected hTNF-α/293 , mRNA of Hek-293 cell and protein expression were detected by RT-PCR and ELISA. The positive group was added hTNF-αfactor,and MTT assay was applied under the optical density 490 nm. Through human tumor cell proliferation inhibition experiment,the inhibitory effects on colon cancer cells ( LOVO) proliferation were observed. Results hTNF-α/293 cells and hTNF-α-positive group showed a significant lower A,which suggested that hTNF-α/293 cells and hTNF-α-positive group had significant inhibition on the proliferation of colon cancer cells. Conclusion The inhibition of hTNF-αsecreted by hTNF-α/293 cells on co-lon cancer cell proliferation shows significant dose-effect dependency,and hTNF-αexpresses a considerable inhibition on the colon cancer cell proliferation as positive drug.

Our studies tried to demonstrate Eha (Et haemolysin activator) could regulate the resistance of the bacterium against acidification to survive in the macrophage and explain its underlying molecular mechanism.When the bacteria infected the macrophages at time intervals,intracellular survival rate in bafilomycin-treated macrophages was higher than that with untreated cells,and the rate of wild type ET 13 was higher than that of its eha mutant,respectively (P＜0.05).The survival rate of the wild type was higher than that of the mutant under acid treatment (P＜0.05).To determine the conditions that induced the highest eha expression,we constructed a pMP220-Peha LacZ plasmid and determined the lacZ expression under different conditions.After exposure of pH6.3 medium for 2 h time,we performed the whole transcriptomic profiles of the wild type and mutant by RNA-sequencing.We identified 147 differentially-expressed genes ([log2 ratio| ≥1),113 and 34 of which were significantly up-and down-regulated,respectively in the mutant,comparing with the wild type.These findings were validated by qRT-PCR.GO functional analysis revealed that these genes were divided into 25 categories,including the bacterial catalysis,cellular composition,combination,localization,metabolism,processing,and transportation.Based on the KEGG database,these genes were distributed in 55 pathways,such as two-component system,ABC transporters,and microbial metabolism in diverse environments.Overall,Eha is an important regulator to affect all kinds of target genes and pathways for E.tarda to adapt to an acid environment.These results could be helpful for further investigations of the mechanisms by which E.tarda survives in macrophages.

Objective To explore the effect of psycho-cardiology treatment on major cardiac events (MACE) incidence in patients after percutaneous coronary intervention (PCI).Methods From November 2014 to November 2015,200 patients with coronary artery intervention were randomly divided into cardiology treatment group (100 cases) and psycho-cardiology group (100 cases).The psycho-cardiology treatment group was conducted to strengthen the psychological counseling and coronary heart disease and the intervention of common sense education and daily reasonable way of life education for 3 months.The scores of psychological assessment were compared between the two groups after 1 year.The patients of the two groups were followed up for 1 year at the same time.The clinical treatment of hypochondriasis rate and MACE incidence rate were observed in the two groups.Results 3 months after PCI,psycho-cardiology treatment group was treated with psychological counseling and coronary heart disease.The psychological assessment score was significantly lower than that in the control group.At 3 months,the HAMD scores of the control group and the psycho-cardiology group were (23.31 ± 5.91) points,(12.40 ± 3.95) points,there was significant difference between the two groups (t =6.235,P ＜ 0.05).At 3 months,the HAMA scores of the control group and the psycho-cardiology group were (22.29 ± 3.93) points,(12.44 ± 4.13) points,there was significant difference between the two groups (t =7.478,P ＜ 0.05),which were significantly decreased compared to admission.Postoperative follow up of 1 year,the clinical medical hypochondriasis rate of psycho-cardiology treatment group was significantly lower than that of cardiology treatment group.The incidence rate of MACE was significantly lower than that of the control group.Conclusion Mental intervention,coronary heart disease and PCI knowledge education can effectively reduce the negative emotion of patients with coronary heart disease after interventional therapy.The incidence of MACE after PCI operation was significantly lower.