Objective:To study the role of basic fibroblast growth f ac tor (bFGF) on angiogenesis in oral squamous cell carcinoma(OSCC).Methods :Expression of bFGF and CD34 in 42 cases of OSCC(14 cases with lymph no de metastasis) and 10 cases of normal oral mucosa(NOM) was detected by immunohis tochemistry. Microvessel density(MVD) in OSCC was assessed with microscope. Results:bFGF was detected in 29(69.05%) cases of OSCC and in 3(33.3 3%) of NOM. MVD in OSCC and NOM was 53.50?9.59 and 23.30?7.33(P

Objective:To approach the role of lymphangiogenesis in oral squamous cell carcinoma(OSCC). Methods: Immunohistochemical double staining technique was utilized to distinguish lymphatic vessels from blood vessels. MLVD was determined by manual counting to analyze the relationship with lymph node metastasis. Results: MLVD was significantly higher in 40 OSCC than 14 normal mucosa(P<0.01). MLVD in OSCC showed a statistically significant relationship with regional lymph node metastasis, depth of invasion(P<0.01). Conclusion: Lymphangiogenesis might occur in growth, infiltration and metastasis of OSCC. Detection of MLVD in OSCC might be important to evaluate tumor metastasis and judge prognosis.

Objective: To study the application of CT and MRI fusion technique in the diagnosis and treatment of intraocular and orbital tumors. Methods: 2D-images of 13 patients with intraocular and orbital tumors were fused by special-point registration and Iterative Local Closest Point(ILCP) method; 3D-fusion images were reconstructed by Ray Tracing method. Results: A 3D-CT-MRI fusion images of intraocular and orbital tumors were reconstructed and displayed. The CT and MRI data of intraocular and orbital tumors were displayed on the same image as a comprehensive whole,which provided a stereogram of 3D-structure of the normal and abnormal orbital tissues. Anatomical structure of the orbit was clearly visualized by 3D-CT-MRI image. Conclusion: The multi-modality fusion technique can provide more accurate and comprehensive information for clinical diagnosis of intraocular and orbital tumors, which is helpful for doctors' planning of surgical operations,clinical education and doctor-patient communication.

OBJECTIVEThe objective of this paper was to study the expression of related protein and Twist transcription factor of epithelial-mesenchymal transition in oral squamous cell carcinoma (OSCC) tissue and the correlations of OSCC and oral squamous cell carcino-metastasis. The paper also investigated the clinical significance of expression on OSCC.

METHODSThe labels of epithelium materialization (E-cadherin and cytokeratin), stromal labels (N-cadherin), transcription factor Twist protein, and mRNA expression in 30 OSCC tissues were investigated via immunohistochemistry and in situ hybridization. The paper also conducted contrast analysis with clinicopathology.

RESULTSImmunization result showed that the expressions of Twist and N-cadherin in the OSCC group were more significant than those of the normal group (P<0.05). The expressions of E-cadherin and keratin in OSCC were significantly lower than those of the normal group (P<0.05). In the moderate- and low-differentiated group of OSCC, the expressions of Twist and N-cadherin were higher than those of the high-differentiated group (P<0.05). The expressions of E-cadherin and keratin were lower than those in the high-differentiated group (P<0.05). In the lymphatic metastasis group, the expressions of Twist and N-cadherin were higher than those of no-lymphatic metastasis group (P<0.05). The expressions of E-cadherin and keratin were lower than those of the no-lymphatic metastasis group (P< 0.05). Results of in situ hybridization showed that the expression of Twist mRNA in the moderate- and low-differentiated groups of OSCC, T3, and T4 groups as well as that of the lymphatic metastasis group were higher than those of the high-differentiated, T1 and T2 groups, and no-separate lymphatic metastasis group, and the differences were statistically significant (P<0.05).

CONCLUSIONEpithelium materialization exists in OSCC tissue. Twist can enhance the invasiveness of tumor cell and promote the infiltration and metastasis of OSCC. The combined detection of Twist, E-cadherin, and N-cadherin expressions can effectively predict and estimate OSCC metastasis.

Cadherins ; Carcinoma, Squamous Cell ; metabolism ; Epithelial Cells ; Epithelial-Mesenchymal Transition ; physiology ; Epithelium ; Humans ; Immunohistochemistry ; Lymphatic Metastasis ; Mouth Neoplasms ; metabolism ; RNA, Messenger ; Twist-Related Protein 1 ; metabolism

Objective To discuss the clinical value of retroperitoneal laparoscopic simple nephrectomy. Methods Retroperitoneal laparoscopic simple nephrectomy was performed in 6 patients, including 5 cases of severe hydronephrosis with non-functioning kidney resulted from ureteral calculi and 1 case of renal tuberculosis. The operation was conducted via retroperitoneal approach. After the upper ureter and the renal pedicle were exposed, the renal artery and vein were clipped and severed. The renal pedicle was occluded only with titanium clips before the removal of the kidney. Results All the operations were performed successfully without complications. The operation time was 130~220 min (mean, 150 min) and the intraoperative blood loss, 80~150 ml (mean, 120 ml). The postoperative hospital stay ranged 5~7 days. Follow-ups for 3 months in the 5 patients with ureterolithiasis revealed normal renal functions. The patient with renal tuberculosis was given anti-tuberculosis therapy and followed for 6 months. No recurrence was seen and the patient’s serum creatinine level was 120 ?mol/L, which was slightly higher than the normal. Conclusions Retroperitoneal laparoscopic simple nephrectomy has advantages of minimal invasion, short hospital stay and rapid recovery. The procedure should be regarded as the “golden standard” for simple nephrectomy.

Objective:To investigate the expression of tumor necrosis factor-?(TNF-?)in the tissues of oral squamous cell carcinoma(OSCC)and its clinical significance. Methods:The expression of TNF-? was assessed by immunohistochemical method of SABC in paraffin tissue sections of 41 cases of OSCC and 10 of normal oral epithelia.Results:The expression of TNF-? in OSCC was higher than that in oral normal mucous tissue(P

Objective:To investigate the expression of Hpa and its correlation with the microvessel density(MVD) in oral squamous-cell carcinoma(OSCC), thereby learning the function of Hpa in the tumor angiogenesis,invasion and lymph node metastasis.Methods:The expression of Hpa was studied in 48 patients with OSCCs, 15 patients with leukoplakia and 10 normal person with healthy oral mucosa by conventional immunohistochemistry and in situ hybridization. MVD was assessed with anti-CD31 antibody. Results: Positive expression ratios of Hpa and Hpa mRNA in leukoplakia were significantly higher than those in normal oral mucosa, while were significantly lower than in OSCC(P

Objective:To study the role of angiopoietin-2(Ang-2) in angiogenesis in oral squamous cell cacinoma(OSCC). Methods:Expression of angiopoietin-2 and CD_34 in 41 cases of OSCC(13 cases with lymph node metastasis)and 10 cases of normal oral mucosa was detected by immunohistochemical SABC method. Microvessel density(MVD) in the samples was counted under microscope. Results:Ang-2 was detected in 28 out of 41(68.29%) cases of OSCC and 2 out of 10(20%) of normal oral mucosa(P

Objective: To investigate the role of vascular cell adhesion molecule-1(VCAM-1)on macrophage infiltration and recruitment in oral squamous cell carcinoma(OSCC). Methods:After detecting the expression of VCAM-1 and macrophage infiltration in OSCC by immunohistochemistry, light microscope was used for counting macrophages. Results:The expression level of VCAM-1 (64.58%)and macrophage numbers(81.04?12.00) was significantly higher in OSCC than those in normal oral mucosa. The expression of VCAM-1 in OSCC was not only related to the tumor infiltration and metastasis in OSCC(P

Objective To identify the metabolites of loganin in rat urine through using ultra high performance liquid chromatography combined with triple TOF mass spectrometry (UPLC-Q-TOF/MS/MS) after oral administration of loganin;To summarize the metabolic pathway of loganin. Methods Rats were given a gavage with loganin. The urine samples were prepared by use of solid phase extraction (SPE). The chromatographic separation was performed on an UPLC C18 column with gradient elution program of acetonitrile and 0.1% formic acid aqueous as mobile phase. Dynamic background subtraction (DBS) technology was employed to trigger information dependent acquisition (IDA) to obtain the characteristic fragment ions of metabolites. Results MetabolitePilot software including several data processing methods were employed to speculate the structures of liganin metabolites and three metabolites were identified. Then, the metabolic pathway of loganin in rats was summarized. Conclusion The analytical method was simple, credible and highly-sensible, which is useful for screening and identifying trace constituents in vivo.