Objective:To explore the executive function differences between internet addicts and Non-addicts. Methods:Internet addicts (n=26) and Non-addicts(n=26) were assessed using HANOI Tower and M-WCST tests。Results:There is a significant difference between internet addicts and non-addicts in neuropsychological tests. The scores of Logical Memory,Visual Memory,Stroop Test,Verbal Fluency in internet addicts were significantly poorer than that of non-addicts in the tests (P

Objective To investigate the clinical application and significance of nasotracheal intubation (NTI)on patients with respiratory failure.Methods 72 cases of post-operative and non-operative patients with respiratory failure were performed with urgent NTI or blind nasotracheal intubation(BNTI)and then mechanical venfilation.Results In all of the cases NTI were successful.32 cases received direct vision NTI;26 cases were BNTI:9 case8 were NTI through fibrobronchoscope and 5 cases were NTI under awake surface anesthesia.Operation were performed within 20 second to 11 minutes[(5.1±3.1)min].The remaining time of tracheal catheter were 2～21d [(9.3±5.9)d].Conclusion NTI was an important measure in rescuing the patients with respiratory failures.It is an essential rescue technique under the condition without fibrobronchoseope.

To investigate the changes of drug resistance of pseudomonas aeruginosa ( PA) to common antimicrobial agents in recent 3 years in Anhui province. The average drug resistance rate was 15. 3%, 17. 0% for piperacillin/tazobactam, amikacin respectively, while the average resistance rate was higher for the third generation cephalospo-rin cefotaxime, ceftriaxone and ceftizoxime, which was between 42. 0% and 55. 0%. The average drug resistance rate was 25. 2% for imipenem. The resistance rates for ceftazidime, cefepime, ceftriaxone and ceftizoxime were sig-nificantly increased( P<0. 05 ) . The resistance of PA to the third generation cephalosporin and ciprofloxacin in-creased gradually, close attention should be paid to it in clinical practice.

BACKGROUND:Sheng-Huangmixture includingChinese medicine Shengmai Decoction and total flavonoids of stems and leaves of radix has been shown to resist inflammation, regulate immune function, and protect ischemic myocardial tissues. However, its effect on the apoptosis of cardiac muscle cels after ischemia/reperfusion injury remains unclear.
OBJECTIVE:To investigate the effects ofSheng-Huangmixture on cardiocyte apoptosis and bax and bcl-2 mRNA expression in rats with ischemia-reperfusion injury.
METHODS:Thirty-six Sprague-Dawley rats weredivided randomly into six groups: low-dose, moderate-dose and high-doseSheng-Huangmixture, positive control, blank and model groups (n=6). After 7 days of administration, models of myocardial ischemia-reperfusion injury were established. TUNEL was usedto detect myocardial apoptosis. RT-PCR was utilized to measure bax and bcl-2 mRNA expression in the ischemic and reperfusion region.
RESULTS AND CONCLUSION:(1) The bcl-2 mRNA expression was significantly higher in the Sheng-Huangmixture group than in the model group (P< 0.05), but bax mRNA expression was significantly lower (P< 0.05). Thus, bcl-2/bax ratio increased. In addition, apoptosis index was more significantly decreased in theSheng-Huangmixture group(P< 0.05). (2) Results demonstrated that Sheng-Huangmixture can protect rat myocardium against ischemia/reperfusion injury, and effectively increase the bcl-2/bax ratio andinhibit the apoptosis of cardiomyocytes, and the underlying mechanism is mediated by up-regulating bcl-2 mRNA expression and down-regulating bax mRNA expression.

LL-37 is the only member of the cathelicidin family known to exist in human acting as an endogenous antibiotic.After removal of the highly conserved N-termini of precursor hCAP18,a 15 000 C-terminal fragment called LL-37 bearing all of the known biological acivity hitherto is liberated by proteinase.LL-37 is active against both Gram-positive and Gram-negtive bacteria.Its antibacterial activity depends on the formation of ?-helical conformation.The cytotoxic activity of LL-37 is inhibited by apolipoprotein A-Ⅰ(apoA-Ⅰ) in the plasma,thereby it can function as a scavenger of LL-37 and prevent host cell damage.In addition to its microbicidal activity,LL-37 has several other functions such as chemotactic,angiogenic action and the ability to bind and neutralize the endotoxin.

OBJECTIVE To investigate the trend of resistance to antimicrobial agents among clinical isolates of Escherichia coli and Klebsiella pneumoniae in 2006-2007.METHODS Agar diffusion test was used to analyze the changes in drug susceptibility of E.coli and K.pneumoniae from 30 hospitals in Anhui in 2006-2007.The resistant rate,intermediate rate and susceptibility rate of drugs were calculated according to the criteria in guidelines of CLSI(2007).RESULTS Carbapenems were the most active antimicrobial agents tested against E.coli and K.pneumoniae.Cefepime,ceftazidime,piperacillin/tazobactam and amikacin showed excellent activity against E.coli and K.pneumoniae.E.coli and K.pneumoniae were still resistant to cefuroxime,ceftriaxone,cefotaxime and aztreonam.ESBLs-producing E.coli strains accounted for 48.9% in the year of 2006 and for 47.2% in the year of 2007.ESBLs-producing K.pneumoniae strains accounted for 36.6% in the year of 2006 and for 40.0% in the year of 2007.CONCLUSIONS Imipenem remains highly active against E.coli and K.pneumoniae.

Objective To explore the effect of recombinant human endostatin combined with transcatheter arterial chemoembolization on vascular endothelial growth factor (VEGF), hypoxia inducible factor-1 alpha(HIF-1α), osteopontin (OPN) and connective tissue growth factor (CTGF) levels in serum of HCC patients.Methods 90 cases of primary liver cancer patients were randomly divided into control group and observation group, 45 cases in each group.The control group received the routine chemotherapy of hepatic artery embolization, and the observation group received recombinant human vascular endostatin combined with hepatic arterial chemoembolization.The serum levels of VEGF, HIF-1α, OPN, and CTGF were detected by enzyme-linked immunosorbent assay before treatment, 3 days and 5 days after operation, then the correlations were analyzed.Results The VEGF, HIF-1α, OPN, CTGF levels after 3 days and 5 days were higher than those before surgery in control group and observation group (P<0.05), the above indexes after 5 days were lower than those after 3 days in both groups (P<0.05), and VEGF, OPN and CTGF levels except for HIF-1αin observation group were significantly lower than those in control group after 5 days (P<0.05).There existed significant positive correlation between serum VEGF and HIF-1α, OPN, CTGF concentration levels(r=0.985, 0.995, 0.959,P<0.05) in 90 cases of liver cancer patients, highly positive correlation between HIF-1αand OPN, CTGF levels (r=0.842,0.874, P<0.05), and moderately positive correlation between OPN and CTGF concentrations (r=0.755,P<0.05).Conclusion The efficacy of recombinant human vascular endostatin combined with transcatheter arterial chemotherapy and embolization in the treatment of primary hepatocellular carcinoma is good.There exsits closely correlation of VEGF, HIF-1α, OPN and CTGF levels in serum, which could reflect clinical efficacy.

Objective To detect whether adipose-derived mesenchymal stem cells (ADMCSs)can differentiate into renal tubular epithelial cells and be used for treatment of acute kidney injury (AKI).Methods ADMSCs were separated and cultured in vitro,and were transfected with lentivirus carrying reporter genes of firefly luciferase and monomeric red fluorescent protein (fluc-mrfp).Transfected ADMCSs (2 × l06) were directly injected into renal parenchyma after establishment of AKI models induced by ischemic reperfusion injury.Distribution and differentiation of ADMSCs in rats were detected by bioluminescence imaging (BLI) and immunofluorescent staining,respectively.Results ADMSCs had positive expressions of CD29 and CD90,rather than expressions of CD34,CD45 and CD31.Lentivirus-transduced ADMSCs had stable expressions of mrfp and fluc reporter genes.BLI showed persistent fluorescence signal in vivo even at day 14 after ADMSCs transplantation.Immunofluorescence staining further indicated that the transplanted ADMSCs could differentiate into tubular epithelial cells.Conclusion ADMSCs in vivo can differentiate into renal tubular epithelial cells,which provides an experimental basis for more researches on ADMSCs transplantation for treatment of AKI.

0.05).The titer of HBV DNA is higher in YMDD mutation group than in no mutation group at twelve month or eighteen month with(8.14?0.94)vs(7.29?0.97) and(8.28?0.77) vs(7.17?0.91) respectively.(t=2.33,P10~8copies/ml).