This paper made an intensive study on existing problems of the current public hospital cost accounting system and proposed amendments by sorting out cost accounting′s content and related concepts.The application value of this paper is,on one hand,to provide a reference for the formulation of cost and expense standards in government accounting standards,and on the other hand,to better its cost accounting method,and to lay system foundation for increasing accuracy efficiency and timeliness of cost accounting.And corresponding proposals were offered to make public hospitals′cost accounting results better applied in hospital management,government pricing and social insurance payment.

Objective: To explore influence of dragon dancing sport on cardiac function and its mechanism. Methods: A total of 20 male students learning in physical education department of Physical Education College of Jishou University were enrolled, randomly and equally divided into dragon dancing sport group (dragon dancing group, received dragon dancing training) and control group (performed daily activity). Indexes of cardiac function were measured by color-code Doppler echocardiography before and 16 weeks after training. Results: Compared with control group after 16-week training, there was significant decrease in heart rate [(66.00±3.39) times/min vs. (60.50±6.02) times/min], significant increase in left ventricular early diastolic peak filling velocity（E）[(82.4±10.17)cm/s vs. (93.01±10.89)cm/s], rate of E/ A（left ventricular late diastolic peak filling velocity）[(1.89±0.24) vs. (2.29±0.47)], stroke volume [(65.5±10.61)ml vs. (75.59±13.35)ml], stroke index [(36.19±6.15)ml/m2 vs. (40.43±8.35) ml/m2], cardiac index [(2.39±0.47)L•min-1•m-2 vs. (2.46±0.65) L•min-1•m-2] in dragon dancing group, P<0.05 all. Conclusion: Dragon dancing sport can significantly improve cardiac function.

Inflammatory bowel disease (IBD) could lead to the complexity of intra-abdominal pathology and patients' malnutrition,which might greatly increase the risk of operation.Laparoscopic minimally invasive surgery is playing an important role in the treatment of IBD,with the advantages of faster postoperative recovery,shorter duration of hospital stay and lower incidence of postoperative complications.And some new techniques for IBD have been reported in the western countries,such as single-incision laparoscopic surgery,natural orifice transluminal endoscopic surgery,robot-assisted laparoscopic surgery and so on.However,the new techniques are not widely used in China,and it is necessary to carry out a series of clinical practices,which should be carefully selected for IBD patients undergoing minimally invasive surgery.

At present,the technology to predict the response to neoadjuvant therapy with biomakers has been widely used in clinical practice.The approaches of biomarkers detection are various,including immunohistochemistry,detection of serum biomarkers conventional blood tests,gene expression profile analysis,single nucleotide polymorphisms,miRNAs,proteomics analysis.With the development of biotechnology,the technology of biomarkers detection is expected to become effective means in assessment of adjuvant therapy,risk,prognosis and individualization in esophageal cancer treatment.

Acupuncture Therapy ; Adult ; Aged ; Female ; Humans ; Male ; Middle Aged ; Neuralgia ; therapy ; Young Adult

Objective To clone acidic mammalian chitinase (AMCase) gene and analyze the sequences of the nucleotides and encoded amino acids. Methods Total RNA was isolated from the stomach of BALB/c mouse and the mRNA was reversely transcribed into cDNA. AMCase gene was amplified by polymerase chain reaction and inserted into recombinant clone vector of pMD18-T. Sequence alignment was performed with DNAMAN 4.0 software. Isoelectric point, amino acid composition, signal peptide and transmembrane helices were analyzed with SignalP 3.0 Server and TMHMM Server v.2.0. Results The cloned gene encodes a protein of 473 amino acids with molecular weight of 51.93 kDa and isoelectric point 4.73. Extensive amino acid sequence homology (up to 99.57%) was found when compared with AMCase. Both protein encoded by this cloned gene and AMCase belonged to family 18 chitinase, which was composed of signal peptide (amino acid position 1-21), the catalytic domain (amino acid position 22-391), hinge region (amino acid position 392-425) and chitin binding domain (amino acid position 426-473). There were two mutations of amino acid in the catalytic domain and hinge region with this AMCase mutant. The amino acid of Ala(position 292)was mutated into amino acid of Pro, and the amino acid of Thr(position 404)was mutated into amino acid of Ser. Conclusion This sequence is a mutant of AMCase gene. Identification of the biological characteristics will play important role in the further study of its biological function by molecular biology technique.

Objective:To study the torsional property and rotary fatigue fracture of Smart K files and R reamers made of nickel-titanium alloy. Methods:The torsional property and rotary fatigue fracture behavior of Smart K files and R reamers were evaluated by means of specially-designed torsional device and simulated endodontic root canals system. The instrument surface and the rotary fatigue fractured surface were investigated by optical microscope and scanning electron microscope. Results:Compared with ANSI/ADA specification No.28, maximum torque values of Smart K and R endodontic instrument were higher. Tensile stresses on the outer surface of the endodontic instruments played a dominant role in fracture initiation during rotary fatigue fracture. The rotary numbers corresponding to rotary fatigue fracture decreased with the increase of the instrument size and curvature. Smart K and R endodontic instruments exhibited good ductility. Dimples were found on the fractured surface, and the fatigue fracture was the typical ductile fracture. Conclusion:Smartendodontic instruments have excellent torsional and rotary fatigue fracture resistance properties.

Objective To study the role of ERK signal pathway in the endochondral ossification of bone mesenchymal stem cells ,and to explore the mechanism of ERK signal pathway in persistent enhanced FGFR2 function on development of mice BMSCs by a knock‐in mouse model with the FGFR2S252W/+ .Methods Mice with neo‐FGFR2 gain‐of‐function mutation were mated with EII‐Cre mice .The genotype of generation mice were identified by PCR and divided into wild type group and mutant type group ac‐cording to their genotype .6 week‐old mice were sacrificed to receive bone mesenchymal stem cells .The western blot was used to compare the level of P‐ERK and ERK and the RT‐PCR was applied to detect the genes of Col2 ,Col10 ,OC ,OP in chondrogenic dif‐ferentiation medium of BMSCs .Then ,treatment of cultured BMSCs with PD98059 ,compare the changes of genes and utilize the in vitro culture of long bones detect the role of ERK signal pathway in the endochondral ossification by FGFR2 mutant .Results We successfully derive BMSCs from FGFR2S252W/+ mutant mice and found the activity of ERK signal pathway of FGFR2S252W/+ was en‐hanced .After been cultured in chondrogenic differentiation medium ,the expressions of the BMSCs mRNA of Col2 ,Col10 from mu‐tant group were decreased ,while the expressions of OC ,OP were increased .Those OC ,OP genes levels showed an increased treated by PD98059 .Using in vitro culture of long bones ,we found the retardation of total length growth of long bones has been rescued by PD98059 treatment ,suggesting that ERK signal pathways was responsible for the retarded long bone development in FGFRS252W/+mice .Conclusion The results indicate these effects are mediated by the ERK signal pathway .Furthermore ,the retardation of long bones has been recued by PD98059 treatment ,suggesting that ERK signal pathway is responsible for the retarded long bone devel‐opment in FGFR2S252W/+ mice .

Objective To evaluate the efficacy of supportive psychotherapy on patients with cardiac neurosis. Method 32 patients with cardiac neurosis were treated with a comprehensive measure consists of adequate drug therapy and supportive psychotherapy. Symptom Checklist 90 (SCL - 90) was rated. Result Among the 9 factors of SCL - 90, scores of somatization, obsessive - compulsive, interpersonal relationship, depression, anxiety and phobic anxiety factors were significanly higher than that of normal controls. After treatment, the scores of somatization, depression, anxiety and phobic anxiety were statistically reduced. Patient- reported improvement efficacy was 84. 38%.Conclusion Adequate drug therapy together with supportive psychotherapy has reliable efficacy on patients with cardiac neurosis.

Objective To assess the effect of fructose sodium diphosphate and alpha-lipoic acid on diabetic peripheral neuropathy. Methods Sixty-six patients with diabetic peripheral neuropathy were randomly divided into two groups, additional to the regular treatment to control blood glucose, 34 patients in the treatment grouptreated with fructse sodium diphosphate 10 g and alpha-lipoic acid 0.45 g daily through intravenous drip for two weeks (3 patients dropped out due to un-tolerance), 32 patients in the control group treated with alpha-lipoic acid 0.45 g dai-ly through intravenous drip for two weeks. All patients were examined on the improvement of symptoms, motor and sensory nerve conduction velocity of the median nerve and nervus peroneus communis before and after the treatment. Results After treatment for two weeks, the overall effect rate in the treatment group was 93.55 % (28/31), which was significantly higher than that in the control group(68.75%,22/32) (P < 0.05). In the treatment group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 47.7 (SD:3.8) m/s and 41.6 (SD: 3.3) m/s after treatment, which was significantly higher than that before treatment (42.5 [SD:2.5] and 36.0[SD:2.8] m/s,respectively) (P <0.05). In the control group, the motor nerve conduction velocity for the median nerve and nervus peroneus communis was 43.4 (SD: 2.0) m/s and 39.6 (SD: 3.3) m/s after treat-ment, which was significantly higher than that before treatment (41.9 [SD16.0] and 36.2 [SD: 1.9] m/s, respec-tively) (P < 0.05). In the treatment group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 40.3 (SD: 1.8) m/s and 34.1 (SD: 2.3) m/s after treatment, which was significantly higher than that before treatment (35.2 [SD:2.3] and 28.8 [SD:2.5] m/s,rospectively) (P <0.05). In the control group,the sensory nerve conduction velocity for the median nerve and nervus peroneus communis was 37.7 (SD:1.7) m/s and 30.8 (SD:2.6) m/s after treatment,which was significantly higher than that before treatment (34.8 [SD :3.1] and 27.8 [SD :2.4] m/s, respectively) (P < 0.05). Furthermore, the improvements in the treat-ment group was significantly higher than those of the control group (P < 0.01). Conclusions Fructse sodium si-phosphate combined with alpha-lipoic acid is more effective to treat diabetic peripheral neuropathy than alpha-lipoic acid only.