Objective: To apply the norms of the portable blood glucose meter in clinic and improve the quality and level of blood glucose detection. Methods:The management organization of the portable blood glucose meter was established. The organization understudied the working principles and the advantages and disadvantages of blood glucose meter. The model of the portable blood glucose meter was unified in a medical unit. The performance of the portable blood glucose meter and the biochemical analyzer was compared. The organization established standard operating procedures, strengthen personnel training. The clinical staff takes quality control regular. Results:The qualified rate of the use, the maintenance and the results detection was increased. Conclusion:The standardized management system guarantees the detection result of the portable blood glucose meter.

To master the performances of the cardiac defibrillator-monitor and enhance its reliability and effi-ciency through quality control. ESA612 electrical safety analyzer and Phase3 defibrillator analyzer were used to test the performances of 56 cardiac defibrillator-monitors, involving in basic items detection, electrical safety detection and performance detection. The qualified items included charging times, internal discharge, ECG monitoring and etc, while the unqualified ones included appearance, electrical safety as well as delay time under synchronous mode. The quality control can improve the safety of the cardiac defibrillator-monitor.

Objective:To discuss the quality control method of injection pump and analyze the factors that affect the quality control of injection pump.Methods: Flow error and blocking alarm error were tested by the INFUTEST 2000E dual-channel infusion pump analyzer according to the national calibration specifications.Results: The syringe specifications, injection speed and injection solvent could influence the test for accuracy of injection pump. The lower injection speed set, the bigger flow test error was, while the smaller the blocking alarm test error was. On the other hand, the higher the injection speed set, the smaller the flow test error was and the bigger the blocking alarm test error was. Besides, the higher qualified rate of flow test was, the lower the qualified rate of blocking alarm test was.Conclusion: The quality control of injection pump should abide by the principle of controllable risk, and should not pursuit too much accuracy.

Mesenchymal stem cells (MSCs) have a strong self-renewal capacity and multi-differentiation potential,which make them become ideal seed cells of tissue repair and target cells of gene therapy.However,the labeling and tracing method of MSCs transplanted in vivo is the hot spot and difficult problem of the current research.At present,the commonly used tracer methods of MSCs include fluorescence dye labeling,molecular marker,imaging marker technology.This paper summarized the advantages and disadvantages of these tracer methods.

Objective To investigate the epidemiological features of fractures caused by earthquake to elevate the quality of prevention and treatment and reduce the mortality and disability rate.Methods A retrospectire study was performed on the data of 1 262 patients with fractures caused by earthquake from May 12,2008 to Aug 31,2008.The following data was statistically analyzed:cause of injury,injured part,associated injuries and treatment.Resultsof the 1 262 cases,11 resulted in deaths;bruise and crush trauma was the major cause (93.98%)of casualties;fractures oflimbs were the main types(55.7%)of fracture;operation was the major treatment.Concluslon Correct treatment is important for fractures caused by earthquake to reduce mortality and disability rate.

Objective To investigate the maternal attitude and demands for Institute of Postpartum Care in order to provide targeted services.Methods 285 women were a investigated about maternal attitude and demands towards Institute of Postpartum Care with self-designed questionnaire.Results 97.9％ (279/285) women thought sitting the month (puerperal period) was very important,50.2％ (143/285)women were interested in Institute of Postpartum Care.They thought the main three advantages were energy saving,medical expertise and technology and avoidance of family conflicts;The three major issues were high price,lack of monitoring mechanism and unfamiliar environment and less family company;33 items of services demands over four points accounted for 78.6％,the three modules with highest scores were cleaning services scored 4.64±0.47,newborn services scored 4.45±0.41,consultation service scored 4.41:±0.53.Conclusions Professional postnatal care services have been a trend and social need,and a large proportion of women have great interest and demand on Institute of Postpartum Care which should make full use of its concentrate resources,and provide specific services according to maternal demands for services.It is urgent to build unified service standards and evaluation indicators to improve service quality.

Objective To investigate the molecular expression and clinical significance of Vimentin and E-cadherin during epithelial-mesenchymal transformation of hepatocellular carcinoma (HCC) tissues.Methods Hepatocellular carcinoma and paracancerous tissue were collected from 55 patients,while nonneoplastic nonnal liver tissue were collected from 20 patients without cancerous diseases as control group.Expressions of Vimentin and E-cadherin were examined by immunohistochemistry staining.Clinicopathological features of the relative expression of Vimentin and E-cadherin,and its influence on prognosis were discussed as well.Results Vimentin was positively expressed in 69.1％ cancer tissue,and was significantly higher than paracancerous tissues and normal liver tissues(P ＜ 0.05).E-cadherin was positively expressed in 27.3％ cancer tissue and was significantly lower compared with paracancerous tissues and normal liver tissues(P ＜0.05).The expression of Vimentin and E-cadherin was negatively correlated(r =-0.653,P ＜ 0.05).Vimentin expressions in subgroups of TNM stage Ⅲ-Ⅳ,poor differentiation,multiple tumor nodules,metastasis and recurrence were all significantly higher than the respective subgroups as TNM stage Ⅰ-Ⅱ] (x2 =7.267,P ＜ 0.05),moderate to well differentiation (x2 =4.045,P ＜ 0.05),single tumor node (x2=12.143,P ＜0.05),without metastasis or recurrence (x2 =7.267,P ＜ 0.05).On the contrary,Ecadherin showed higher expression in the subgroup of single tumor node compared with multiple tumor nodules(x2 =6.878,P ＜ 0.05).The follow-up of patients further demonstrated that patients with positive Vimentin expression had a significantly lower disease-free survival compared with the Vimentin negative group (P ＜0.05).Conclusions Vimentin and E-cadherin both participated in epithelial-mesenchymal transition process of hepatocellular carcinoma.High expression of Vimentin and low expression of E-cadherin were closely correlated with occurrence and development of HCC.Vimentin and E-cadherin may serve as impottant biomarkers in clinical diagnosis and prognosis prediction of HCC.

Objective To analyze the constituent ratio and clinical characteristics of spine trauma and spinal cord injury of the survivors in the Sichuan earthquake.Methods198 cases wounded in the Sichuan earthquake were divided into four groups according to age for collecting the type of fracture, nerve damage, spinal fractures combined injuries of data from multiple sites, analyzing their clinical characteristics.ResultsFracture types are most common compression fractures (49.3%), followed by the burst fractures (45.9%). 105 of them had spinal cord injury, ASIA grade: A grade: 20 cases, B Grade: 18 cases, C grade: 25 cases, D Grade: 42 cases. 41 cases had combined injury. T12, L1, and L2 vertebral fractures were common types.ConclusionCompression fractures and burst fractures as spinal injuries occurred commonly in Sichuan earthquake mainly in the 18～65 years old, and spinal cord injury accounted for 53.03% spinal injury.

Objective To explore the non-HLA gene polymorphisms that influence CMV infection after hematopoietic stem cell transplantation (HSCT).Method Non-HLA gene (ACE,CD14,MPO,MBL) single nucleotide polymorphisms were determined by using sequence-specific primer polymerase chain reaction (PCR-SSP) and sequencing in 64 pairs of donors and recipients before HSCT and the differences of non-HLA gene were analysed in CMV positive and negative patients Results The distribution of ACE gene single nucleotide polymorphism was DD (14/128,10.9％),ID (72/128,56.3％),and Ⅱ (42/128,38.8％).The distribution of CD14-159 allele gene single nucleotide polymorphism was CC (18/128,14.1％),CT (81/128,63.3％),and TT (29/128,22.7％).The distribution of MPO-463 allele gene single nucleotide polyrnorphism was G (100)/128,78.1％),A (2/128,1.6％),and GA (26/128,20.3％).The distribution of MBL gene single nucleotide polymorphism was H (28/128,21.9％),HL (73/128,57.0％),L (27/128,21.1％),Y (87/128,68.0％),YX (38/128,29.7％),X (3/128,2.3％),A (94/128,73.4％),AB (32/ 128,25.0％),and B (2/128,1.6％).The allele frequency of ACE,CD14 and MPO shoed no significant differcence between CMV positive and negative patients The gene frequency of MBL-HL was increased in CMV positive group.Conclusion MBL gene single nucleotide polymorphisrns may influence CMV infection after HSCT.

Objective To compare the theory of mind(TOM) abilities between autistic children's parents and the normal children' s,and to indirectly prove that TOM deficit in autism maybe related to heredity in cognitive neuropsychology.Methods This study examines TOM abilities in 32 autistic children' s parents and 32 normal children' s parents,using the computerized Yoni task and an extensive battery of neuropsychological tests.Results In contrast to findings in the control group,autistic children' s parents showed significantly lower scores in affective theory of mind( (23.06 ±5.12)vs(25.63 ±4.47),P＜0.01 ) and special emotion( ( 10.13 ±4.14)vs ( 14.87 ±2.21 ),P＜0.05),but not in cognitive theory of mind( (22.75 ±5.01 ) vs(22.56 ±5.63),P＞0.05).Conclusion The autistic children' s parents are impaired in affective theory of mind,and to indirectly prove that autistic TOM deficit maybe related to heredity in cognitive neuropsychology.