Objective To investigate and compare the clinical efficacy and safety of laparoscopic and traditional open surgery in the rectal cancer surgery.Methods From March 2007 to February 2013,86 patients with radical resection of rectal carcinoma in General Surgery Department of Pinggu District Hospital of Beijing were selected as research objects,according to random number table method,they were divided into laparoscopic group(n=43) and laparotomy group(n=43).The laparoscopic group was given laparoscopic radical resection of rectal cancer,the laparotomy group was given traditional open surgery.The baseline data,operative incision length,intraoperative blood loss,operative time,postoperative hospital stay,total number of lymph nodes cleaning,exhaust time,time to get out of bed,hospitalization expenses,VAS score and postoperative complications between the two groups were observed and compared.And the survival rates of the two groups were compared.Results.The operative incision length of laparoscopic group was shorter than laparotomy group((4.20±1.15) cm vs.(16.53±4.62) cm),the intraoperative blood loss was less than laparotomy group((167.43±16.24) ml vs.(220.74±22.53) ml),postoperative hospital stay,exhaust time,time to get out of bed were shorter than laparotomy group((8.26±2.09) d vs.(13.52±2.97) d,(3.24±1.42) d vs.(4.23±1.60) d,(3.07±0.84) d vs.(4.23±1.29) d),the hospitalization expenses was higher than laparotomy group(all P<0.05).The VAS score and postoperative complication rate of laparoscopic group were lower than laparotomy group((5.32±1.18) points vs.(8.16±1.25) points,2.33%(1/43) vs.20.93%(9/43),P<0.05).There were no significant differences inoperative time and total number of lymph nodes cleaning between the two groups(P>0.05).The 5 year overall survival rate of laparoscopic group was 52.6%,the laparotomy group was 47.4%,there was no significant difference between the two groups(χ2=0.465,P>0.05).Conclusion The clinical efficacy of laparoscopic and traditional open surgery in the rectal cancer surgery are the same,and laparoscopic radical surgery is safe and effective,it has the advantages of less trauma,less complications and quick recovery,is worth popularizing.

Objective To investigate the charge nurses' leadership style perceived by nurses and nurse's self-efficacy,and then analyze the relationship between them.Methods Totally 368 staff nurses from 3A hospitals in Sichuan were investigated with questionnaires.The results underwent analysis.Results The score of charge nurses' transformational leadership style was 58.06±12.10,from high to low in turn was intellectual stimulation (3.10±0.69),inspirational motivation (2.92±0.73),idealized influence (2.88±0.67) and individualized consideration (2.71±0.74).The score of charge nurses' transactional leadership style was 35.77±8.58,from high to low in turn was contingency reward (3.11±0.71),passive exemplary management (3.09±0.75) and active exemplary management (2.75±0.90).The score of nurses' self-efficacy was 83.51±11.62.The transformational leadership style had positively predictive impact on nurses' selfefficacy.Conclusions Nursing managers should apply transformational leadership to improve nurses selfefficacy,promote the physical and mental health of nurses and the long-term development of hospital.

Objective To investigate the calbindin D\|28K (CB) neurons that receive the visceral nociceptive information in the interstitial nucleus of the spinal trigeminal tract (INV) directly project to the nucleus of the solitary tract (NTS). Methods Triple\|labeled methods of fluorogold (FG) retrograde tracing combined with Fos and CB proteins immunofluorescence histochemistry after formalin stimulating upper alimentary tract. Results Most of FG\|retrograde labeled neurons distributed in the paratrigeminal nucleus (PaV) and the dorsal paramarginal nucleus (PaMd) of INV ipsilateral to the FG injection. About 71\^2% of FG\|retrograde labeled neurons contained CB and 31\^5% of FG/CB double\|labeled neurons exhibited positive Fos\|immunoreaction in INV.Conclusion The results suggested that a part of neurons containing CB in INV receive the visceral nociceptive information and directly project to NTS. CB neurons might play an important role in transmitting visceral nociceptive information from INV to NTS. [

Objective　To investigate the influence of pretr eatment with anti-B lymphocyte serum on the immunogenecity and endocrine functi on of islet preparations.Methods　Porcine ICCs were pretre ated with anti-B lymphocyte serum which was produced using the mixed adult porc i ne B lymphocyte as antigen.The immunogenecity of islet was measured by mixed isl et lymphocyte culture (MILC) and SLA-DR positive cell number within the islet and the islet function was assayed with insulin release test.Results 　The cpm value of MILC of the group pretreated with anti-B lymphocy te serum and the MHCⅠ antigen and SLA-DR antigen positive cell numbers was mar kedly decreased in comparison with that of control group (P＜0.001).There wa s no significant difference in insulin release of additional different groups.[ WT5”HZ Conclusion　The pretreatment of islet preparation with anti -B lymphocyte serum may decrease the immunogenecity of islet preparation,while there is no marked influence on its insulin release function.

Objective To study the feasibility of laparoscopic total mesorectal excision(TME) for low rectal cancer.Methods Laparoscopic total mesorectal excision was performed in 126 patients with low rectal cancer according to TME principle.Results The operation time was 95 - 180 min,with an average time of ( 117 ± 21 ) min,the amount of bleeding 50 - 200 ml,with an average amount of (90 ± 27 ) ml.2 - 3 days after surgery,gastrointestinal function was restored.Hospital stay was 6 - 14 days,with an average of (8 ± 2)days.Four cases converted to open surgery,the conversion rate for laparotomy was 3.2％.The proportion of sphincter-preserving operation was 95.24％ (120/126).No instant or delayed injury of ureters,large bleeding in front of sacrum and other operation-related severe complications happened intra-and after operation.Conclusion Laparoscopic surgery for low rectal cancer is safe and feasible.

Objective To analyze the trend of nursing information system(Nursing Information System,NIS) and contemporary situation,so as to provide a reference for the further development of NIS in China.Methods Based on China Academic Journal Network Publishing Database,Wanfang database and VIP Chinese academic periodical database,retrieval published NIS literature from 2009 to 2013 in China.Seven aspects were analyzed:time,areal distribution,cooperative rate,authors,institutions,periodicals and keywords.Results There were 438 articles from 128 published journals,47.9％ papers were in the statistical source journals.Research content mainly involved clinical nursing and nursing management.Conclusions NIS research has achieved great development in the past 5 years,but the researches should be strengthened in breadth and depth.

Objective To investigate the tissue structure of the human scalp hair follicles,the ability of hair follicle regeneration in vitro and the influence elements of growth and proliferation of follicle organ.Methods The paraffin sections of the 15 cases of human scalps and 150 cases of scalp hair follicle tissues were prepared,and morphological characteristics and organizational structures was observed by HE staining.The experience and growth pattern of the hair follicle that was cultured in Williams E medium in vitro were summarized.Results Human scalp contained a large number of hair follicles and sebaceous glands,sweat glands,and other skin accessories.Epithelial cells were rich in hair follicles and poorly differentiated in structure,and grew in the regular direction in scalp tissue.The Williams E was used to culture successfully human scalp hair follicles.Hair follicles grew with the fastest speed in the first 5 days.Compared with other media,the use of serum-free Williams E medium in the hair follicle in vitro experiments achieved better results.Conclusions Human scalp contained a large number of hair follicles and sebaceous glands,sweat glands,and other skin accessories.Williams E medium without serum medium was more conducive than other medium for hair follicle culture in vitro,so that regeneration of hair follicles in vitro with a better capacity created favorable conditions for the delay in hair transplant surgery.

Objective ABCG2(ATP-binding cassette superfamily G member 2)is a member of ABC transporter superfamily,and participates in the mechanisms of high resistance to chemotherapeutic agents in tumor treatment.The aim of present study is to investigate the expression and significance of ABCG2 in lung cancer tissues.Methods Lung cancer tissues were obtained from 83 patients(64 NSCLC,19 SCLC)by bio-autopsy or surgery.No patients had received chemotherapy or radiotherapy before bio-autopsy or surgery.83 lung cancer specimens were analyzed for ABCG2 protein expression by using immunohistochemistry.Negative controls had the primary antibody eliminated.Human placenta tissues were used as positive control.In addition,five specimens of normal lung tissues were included for comparative study.Results The present study confirmed the predominant localization of ABCG2 transporter in plasma membrane.Some of ABCG2-positive tumors showed mixed membranous and cytoplasmic staining.ABCG2 expression was found in 71.9%(46/64)of NSCLC and 10.5%(2/19)of SCLC.The level of ABCG2 expression was significantly higher in NSCLC than in SCLC(P

Objective To investigate the influence of pretreatment with anti-B lymphocyte serum on the immunogenecity and endocrine function of islet preparations.Methods Porcine ICCs were pretreated with anti-B lymphocyte serum which was produced using the mixed adult porcine B lymphocyte as antigen.The immunogenecity of islet was measured by mixed islet lymphocyte culture (MILC) and SLA-DR positive cell number within the islet and the islet function was assayed with insulin release test.Results The cpm value of MILC of the group pretreated with anti-B lymphocyte serum and the MHCⅠ antigen and SLA-DR antigen positive cell numbers was markedly decreased in comparison with that of control group ( P

0.05). The duration of normoglycemia in experimental group was 23～65 days (mean 48 days), while 3～6 days (mean 5 days) in control group, with the difference being very significant between the two groups (P